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Q86504

- NSP3_ROTRF

UniProt

Q86504 - NSP3_ROTRF

Protein

Non-structural protein 3

Gene
N/A
Organism
Rotavirus A (strain Cow/France/RF/1975 G6-P6[1]-I2-R2-C2-M2-A3-N2-T6-E2-H3) (RV-A)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 45 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Involved in translation of viral mRNAs and shutoff of host protein synthesis. It is unclear whether it is required for the translation of viral mRNAs as well. Functions similarly to, and competes with the cellular poly(A)-binding protein PABPC1. Binds the conserved sequence 'GACC' at the 3' end of viral mRNAs and allows circularization of viral mRNAs in translation. Interacts with ZC3H7B/RoXaN and with the eukaryotic translation initiation factor eIF4G, using the same region employed by PABP-C1. NSP3 thus displaces PABPC1 from eIF4G, inhibiting the translation of cellular poly(A) mRNAs. Also responsible for the nuclear relocalization of PABPC1 upon rotavirus infection.

    GO - Molecular functioni

    1. RNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. regulation of translation Source: UniProtKB-KW
    2. suppression by virus of host gene expression Source: UniProtKB-KW

    Keywords - Biological processi

    Eukaryotic host gene expression shutoff by virus, Eukaryotic host translation shutoff by virus, Host gene expression shutoff by virus, Host-virus interaction, Translation regulation

    Keywords - Ligandi

    RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Non-structural protein 3
    Short name:
    NSP3
    Alternative name(s):
    NCVP4
    Non-structural RNA-binding protein 34
    Short name:
    NS34
    OrganismiRotavirus A (strain Cow/France/RF/1975 G6-P6[1]-I2-R2-C2-M2-A3-N2-T6-E2-H3) (RV-A)
    Taxonomic identifieri10933 [NCBI]
    Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
    Virus hostiBos taurus (Bovine) [TaxID: 9913]
    ProteomesiUP000007179: Genome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 313313Non-structural protein 3PRO_0000367816Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer. Interacts (via the coiled-coil region) with host ZC3H7B (via LD motif). Interacts with host EIF4G1.2 Publications

    Protein-protein interaction databases

    DIPiDIP-1160N.

    Structurei

    3D structure databases

    ProteinModelPortaliQ86504.
    SMRiQ86504. Positions 7-163, 206-312.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni4 – 149146RNA-bindingBy similarityAdd
    BLAST
    Regioni150 – 20657DimerizationBy similarityAdd
    BLAST
    Regioni170 – 23465Interaction with ZC3H7BAdd
    BLAST
    Regioni208 – 313106Interaction with EIF4G1By similarityAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili166 – 23772Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the rotavirus A NSP3 family.Curated

    Keywords - Domaini

    Coiled coil

    Family and domain databases

    InterProiIPR002873. Rotavirus_NSP3.
    [Graphical view]
    PfamiPF01665. Rota_NSP3. 1 hit.
    [Graphical view]
    SUPFAMiSSF69903. SSF69903. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q86504-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLKMESTQQM ASSIINTSFE AAVVAATSTL ELMGIQYDYN EVYTRVKSKF    50
    DYVMDDSGVK NNLLGKAATI DQALNGKFGS AARNRNWMAD TRTTARLDED 100
    VNKLRMMLSS KGIDQKMRVL NACFNVKRVP GKSSSIIKCT RLMRDKIERG 150
    EVEVDDSFVE EKMEVDTIDW KSRYEQLEKR FESLKQRVNE KYTSWVQKAK 200
    KVNENMYSLQ NVISQQQSQI ADLQNYCNKL EVDLQNKISS LVSSVEWYLK 250
    SMELPDEIKT DIEQQLNSID VINPINAIDD FESLIRNIIL DYDRIFLMFK 300
    GLMRQCNYEY TYE 313
    Length:313
    Mass (Da):36,347
    Last modified:November 1, 1996 - v1
    Checksum:i2AD38815AAEB349B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z21639 mRNA. Translation: CAA79754.1.
    PIRiS49006.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z21639 mRNA. Translation: CAA79754.1 .
    PIRi S49006.

    3D structure databases

    ProteinModelPortali Q86504.
    SMRi Q86504. Positions 7-163, 206-312.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-1160N.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR002873. Rotavirus_NSP3.
    [Graphical view ]
    Pfami PF01665. Rota_NSP3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF69903. SSF69903. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Expression of two bovine rotavirus non-structural proteins (NSP2, NSP3) in the baculovirus system and production of monoclonal antibodies directed against the expressed proteins."
      Aponte C., Mattion N.M., Estes M.K., Charpilienne A., Cohen J.
      Arch. Virol. 133:85-95(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "RoXaN, a novel cellular protein containing TPR, LD, and zinc finger motifs, forms a ternary complex with eukaryotic initiation factor 4G and rotavirus NSP3."
      Vitour D., Lindenbaum P., Vende P., Becker M.M., Poncet D.
      J. Virol. 78:3851-3862(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH HUMAN ZC3H7B.
    3. "Nuclear localization of cytoplasmic poly(A)-binding protein upon rotavirus infection involves the interaction of NSP3 with eIF4G and RoXaN."
      Harb M., Becker M.M., Vitour D., Baron C.H., Vende P., Brown S.C., Bolte S., Arold S.T., Poncet D.
      J. Virol. 82:11283-11293(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH HUMAN ZC3H7B, INTERACTION WITH HUMAN EIF4G1.

    Entry informationi

    Entry nameiNSP3_ROTRF
    AccessioniPrimary (citable) accession number: Q86504
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 24, 2009
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 45 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3