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Q86504

- NSP3_ROTRF

UniProt

Q86504 - NSP3_ROTRF

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Protein

Non-structural protein 3

Gene
N/A
Organism
Rotavirus A (strain Cow/France/RF/1975 G6-P6[1]-I2-R2-C2-M2-A3-N2-T6-E2-H3) (RV-A)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in translation of viral mRNAs and shutoff of host protein synthesis. It is unclear whether it is required for the translation of viral mRNAs as well. Functions similarly to, and competes with the cellular poly(A)-binding protein PABPC1. Binds the conserved sequence 'GACC' at the 3' end of viral mRNAs and allows circularization of viral mRNAs in translation. Interacts with ZC3H7B/RoXaN and with the eukaryotic translation initiation factor eIF4G, using the same region employed by PABP-C1. NSP3 thus displaces PABPC1 from eIF4G, inhibiting the translation of cellular poly(A) mRNAs. Also responsible for the nuclear relocalization of PABPC1 upon rotavirus infection.

GO - Molecular functioni

  1. RNA binding Source: UniProtKB-KW

GO - Biological processi

  1. suppression by virus of host translation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Eukaryotic host gene expression shutoff by virus, Eukaryotic host translation shutoff by virus, Host gene expression shutoff by virus, Host-virus interaction, Translation regulation

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Non-structural protein 3
Short name:
NSP3
Alternative name(s):
NCVP4
Non-structural RNA-binding protein 34
Short name:
NS34
OrganismiRotavirus A (strain Cow/France/RF/1975 G6-P6[1]-I2-R2-C2-M2-A3-N2-T6-E2-H3) (RV-A)
Taxonomic identifieri10933 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostiBos taurus (Bovine) [TaxID: 9913]
ProteomesiUP000007179: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 313313Non-structural protein 3PRO_0000367816Add
BLAST

Interactioni

Subunit structurei

Homodimer. Interacts (via the coiled-coil region) with host ZC3H7B (via LD motif). Interacts with host EIF4G1.2 Publications

Protein-protein interaction databases

DIPiDIP-1160N.

Structurei

3D structure databases

ProteinModelPortaliQ86504.
SMRiQ86504. Positions 7-163, 206-312.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni4 – 149146RNA-binding By similarityAdd
BLAST
Regioni150 – 20657Dimerization By similarityAdd
BLAST
Regioni170 – 23465Interaction with ZC3H7BAdd
BLAST
Regioni208 – 313106Interaction with EIF4G1 By similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili166 – 23772 Reviewed predictionAdd
BLAST

Sequence similaritiesi

Belongs to the rotavirus A NSP3 family.

Keywords - Domaini

Coiled coil

Family and domain databases

InterProiIPR002873. Rotavirus_NSP3.
[Graphical view]
PfamiPF01665. Rota_NSP3. 1 hit.
[Graphical view]
SUPFAMiSSF69903. SSF69903. 1 hit.

Sequencei

Sequence statusi: Complete.

Q86504-1 [UniParc]FASTAAdd to Basket

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MLKMESTQQM ASSIINTSFE AAVVAATSTL ELMGIQYDYN EVYTRVKSKF    50
DYVMDDSGVK NNLLGKAATI DQALNGKFGS AARNRNWMAD TRTTARLDED 100
VNKLRMMLSS KGIDQKMRVL NACFNVKRVP GKSSSIIKCT RLMRDKIERG 150
EVEVDDSFVE EKMEVDTIDW KSRYEQLEKR FESLKQRVNE KYTSWVQKAK 200
KVNENMYSLQ NVISQQQSQI ADLQNYCNKL EVDLQNKISS LVSSVEWYLK 250
SMELPDEIKT DIEQQLNSID VINPINAIDD FESLIRNIIL DYDRIFLMFK 300
GLMRQCNYEY TYE 313
Length:313
Mass (Da):36,347
Last modified:November 1, 1996 - v1
Checksum:i2AD38815AAEB349B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z21639 mRNA. Translation: CAA79754.1.
PIRiS49006.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z21639 mRNA. Translation: CAA79754.1 .
PIRi S49006.

3D structure databases

ProteinModelPortali Q86504.
SMRi Q86504. Positions 7-163, 206-312.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-1160N.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR002873. Rotavirus_NSP3.
[Graphical view ]
Pfami PF01665. Rota_NSP3. 1 hit.
[Graphical view ]
SUPFAMi SSF69903. SSF69903. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Expression of two bovine rotavirus non-structural proteins (NSP2, NSP3) in the baculovirus system and production of monoclonal antibodies directed against the expressed proteins."
    Aponte C., Mattion N.M., Estes M.K., Charpilienne A., Cohen J.
    Arch. Virol. 133:85-95(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "RoXaN, a novel cellular protein containing TPR, LD, and zinc finger motifs, forms a ternary complex with eukaryotic initiation factor 4G and rotavirus NSP3."
    Vitour D., Lindenbaum P., Vende P., Becker M.M., Poncet D.
    J. Virol. 78:3851-3862(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH HUMAN ZC3H7B.
  3. "Nuclear localization of cytoplasmic poly(A)-binding protein upon rotavirus infection involves the interaction of NSP3 with eIF4G and RoXaN."
    Harb M., Becker M.M., Vitour D., Baron C.H., Vende P., Brown S.C., Bolte S., Arold S.T., Poncet D.
    J. Virol. 82:11283-11293(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH HUMAN ZC3H7B, INTERACTION WITH HUMAN EIF4G1.

Entry informationi

Entry nameiNSP3_ROTRF
AccessioniPrimary (citable) accession number: Q86504
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: November 1, 1996
Last modified: July 9, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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