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Q864Z3

- S22A6_BOVIN

UniProt

Q864Z3 - S22A6_BOVIN

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Protein

Solute carrier family 22 member 6

Gene

SLC22A6

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Involved in the renal elimination of endogenous and exogenous organic anions. Functions as organic anion exchanger when the uptake of one molecule of organic anion is coupled with an efflux of one molecule of endogenous dicarboxylic acid (glutarate, ketoglutarate, etc). Mediates the sodium-independent uptake of p-aminohippurate (PAH), 2,3-dimercapto-1-propanesulfonic acid (DMPS), cidofovir, adefovir, 9-(2-phosphonylmethoxyethyl) guanine (PMEG), 9-(2-phosphonylmethoxyethyl) diaminopurine (PMEDAP), ochratoxin (OTA), acyclovir (ACV), 3'-azido-3-'deoxythymidine (AZT), cimetidine (CMD), 2,4-dichloro-phenoxyacetate (2,4-D), hippurate (HA), indoleacetate (IA), indoxyl sulfate (IS) and 3-carboxy-4-methyl-5-propyl-2-furanpropionate (CMPF) and edaravone sulfate. PAH uptake is inhibited by p-chloromercuribenzenesulphonate (PCMBS), diethyl pyrocarbonate (DEPC), indomethacin, sulindac, diclofenac, carprofen, okadaic acid, benzothiazolylcysteine (BTC), S-chlorotrifluoroethylcysteine (CTFC), cysteine S-conjugates S-dichlorovinylcysteine (DCVC), furosemide, steviol, phorbol 12-myristate 13-acetate (PMA), calcium ionophore A23187, benzylpenicillin, bumetamide, losartan, probenecid, phenol red, urate, glutarate and alpha-ketoglutarate (By similarity).By similarity

GO - Molecular functioni

  1. inorganic anion exchanger activity Source: UniProtKB
  2. organic anion transmembrane transporter activity Source: UniProtKB

GO - Biological processi

  1. alpha-ketoglutarate transport Source: UniProtKB
  2. organic anion transport Source: UniProtKB
  3. renal tubular secretion Source: UniProtKB
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Solute carrier family 22 member 6
Alternative name(s):
Organic anion transporter 1
Renal organic anion transporter 1
Short name:
ROAT1
Gene namesi
Name:SLC22A6
Synonyms:OAT1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Unplaced

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2323CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei24 – 4421HelicalSequence AnalysisAdd
BLAST
Topological domaini45 – 13591ExtracellularSequence AnalysisAdd
BLAST
Transmembranei136 – 15621HelicalSequence AnalysisAdd
BLAST
Topological domaini157 – 1648CytoplasmicSequence Analysis
Transmembranei165 – 18521HelicalSequence AnalysisAdd
BLAST
Topological domaini186 – 19510ExtracellularSequence Analysis
Transmembranei196 – 21621HelicalSequence AnalysisAdd
BLAST
Topological domaini217 – 2248CytoplasmicSequence Analysis
Transmembranei225 – 24521HelicalSequence AnalysisAdd
BLAST
Topological domaini246 – 2483ExtracellularSequence Analysis
Transmembranei249 – 26921HelicalSequence AnalysisAdd
BLAST
Topological domaini270 – 33768CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei338 – 35821HelicalSequence AnalysisAdd
BLAST
Topological domaini359 – 36810ExtracellularSequence Analysis
Transmembranei369 – 38921HelicalSequence AnalysisAdd
BLAST
Topological domaini390 – 3956CytoplasmicSequence Analysis
Transmembranei396 – 41621HelicalSequence AnalysisAdd
BLAST
Topological domaini417 – 4259ExtracellularSequence Analysis
Transmembranei426 – 44621HelicalSequence AnalysisAdd
BLAST
Topological domaini447 – 45610CytoplasmicSequence Analysis
Transmembranei457 – 47721HelicalSequence AnalysisAdd
BLAST
Topological domaini478 – 4847ExtracellularSequence Analysis
Transmembranei485 – 50521HelicalSequence AnalysisAdd
BLAST
Topological domaini506 – 54944CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. basolateral plasma membrane Source: UniProtKB
  2. integral component of plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 549549Solute carrier family 22 member 6PRO_0000324165Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi56 – 561N-linked (GlcNAc...)Sequence Analysis
Glycosylationi92 – 921N-linked (GlcNAc...)Sequence Analysis
Glycosylationi113 – 1131N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

Glycosylated. Glycosylation is necessary for proper targeting of the transporter to the plasma membrane (By similarity).By similarity

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiQ864Z3.

Structurei

3D structure databases

ProteinModelPortaliQ864Z3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

Multiple cysteine residues are necessary for proper targeting to the plasma membrane.By similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0477.
HOGENOMiHOG000234569.
HOVERGENiHBG108433.
InParanoidiQ864Z3.
KOiK08203.

Family and domain databases

InterProiIPR020846. MFS_dom.
IPR016196. MFS_dom_general_subst_transpt.
IPR004749. Orgcat_transp.
IPR005828. Sub_transporter.
[Graphical view]
PfamiPF00083. Sugar_tr. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
TIGRFAMsiTIGR00898. 2A0119. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q864Z3-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAFNDLLLQL GGVGRFQKIQ VTLVILPLIL LASHNTLQNF TAAIPTHHCR
60 70 80 90 100
PPADTNLSED GDLEAWLPRD GQGRPESCLL FTSPQRGPPF PNGTETNGTG
110 120 130 140 150
ATEPCPHGWI YDNSTFPSTI VTEWDLVCSH RALRQLAQSL YMMGVLLGAM
160 170 180 190 200
TFGCLADRLG RRKVLIFNYL QTAVSGTCAA FAPNFPAYCA FRFLSGMSTA
210 220 230 240 250
GVVLNCMTLN VEWMPIHTRA YVGTLTGYVY SLGQFLLAGM AYAVPHWRYL
260 270 280 290 300
QLLVSAPFFA FFIYSWFFIE SARWYASSGR LDLTLRNLQR VAWINGKQEE
310 320 330 340 350
GANLSMEALQ ASLKKELTTG KSQASALELI RCPALRRLFL CLSMLWFATS
360 370 380 390 400
FAYYGLVMDL QGFGVSIYLI QVIFGAVDLP AKLVSFLVIN NVGRRPAQMA
410 420 430 440 450
SLLLAGICIL INGVVPKDKS IVRTSLAVLG KGCLASSFNC IFLYTGEVYP
460 470 480 490 500
TMIRQTGLGM GSTLARVGSI VSPLVSMTAE LYPSVPLFIY GAVPVAASAA
510 520 530 540
IALLPETLGQ PLPDTVQDVE NRRRGKTRKQ QEELQKQMVP LQASAQVKN
Length:549
Mass (Da):60,184
Last modified:March 18, 2008 - v2
Checksum:i8D4793CC3C408B69
GO
Isoform 2 (identifier: Q864Z3-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-130: MAFNDLLLQL...VTEWDLVCSH → MGRGGPSPAS...TAPSLPPSSL

Note: No experimental confirmation available.

Show »
Length:472
Mass (Da):51,133
Checksum:iBD43DF87C6BD8EE0
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti162 – 1621R → Q in CAD71145. 1 PublicationCurated
Sequence conflicti162 – 1621R → Q in AAX46607. (PubMed:16305752)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 130130MAFND…LVCSH → MGRGGPSPASSSPPPSGDRP FPMAQRPTAQGPQSPVPTAG STTTAPSLPPSSL in isoform 2. 1 PublicationVSP_032167Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ549816 mRNA. Translation: CAD71145.1.
BT021760 mRNA. Translation: AAX46607.1.
BC151704 mRNA. Translation: AAI51705.1.
RefSeqiNP_001001143.1. NM_001001143.2.
UniGeneiBt.28372.

Genome annotation databases

GeneIDi407180.
KEGGibta:407180.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ549816 mRNA. Translation: CAD71145.1 .
BT021760 mRNA. Translation: AAX46607.1 .
BC151704 mRNA. Translation: AAI51705.1 .
RefSeqi NP_001001143.1. NM_001001143.2.
UniGenei Bt.28372.

3D structure databases

ProteinModelPortali Q864Z3.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi Q864Z3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 407180.
KEGGi bta:407180.

Organism-specific databases

CTDi 9356.

Phylogenomic databases

eggNOGi COG0477.
HOGENOMi HOG000234569.
HOVERGENi HBG108433.
InParanoidi Q864Z3.
KOi K08203.

Miscellaneous databases

NextBioi 20818436.

Family and domain databases

InterProi IPR020846. MFS_dom.
IPR016196. MFS_dom_general_subst_transpt.
IPR004749. Orgcat_transp.
IPR005828. Sub_transporter.
[Graphical view ]
Pfami PF00083. Sugar_tr. 1 hit.
[Graphical view ]
SUPFAMi SSF103473. SSF103473. 1 hit.
TIGRFAMsi TIGR00898. 2A0119. 1 hit.
PROSITEi PS50850. MFS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of the organic anion transporter 1 from bovine kidney."
    Geyer J., Petzinger E.
    Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Kidney.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  3. NIH - Mammalian Gene Collection (MGC) project
    Submitted (AUG-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: Hereford.
    Tissue: Kidney.

Entry informationi

Entry nameiS22A6_BOVIN
AccessioniPrimary (citable) accession number: Q864Z3
Secondary accession number(s): A7E342
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: March 18, 2008
Last modified: October 1, 2014
This is version 84 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3