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Protein

Solute carrier family 22 member 6

Gene

SLC22A6

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in the renal elimination of endogenous and exogenous organic anions. Functions as organic anion exchanger when the uptake of one molecule of organic anion is coupled with an efflux of one molecule of endogenous dicarboxylic acid (glutarate, ketoglutarate, etc). Mediates the sodium-independent uptake of p-aminohippurate (PAH), 2,3-dimercapto-1-propanesulfonic acid (DMPS), cidofovir, adefovir, 9-(2-phosphonylmethoxyethyl) guanine (PMEG), 9-(2-phosphonylmethoxyethyl) diaminopurine (PMEDAP), ochratoxin (OTA), acyclovir (ACV), 3'-azido-3-'deoxythymidine (AZT), cimetidine (CMD), 2,4-dichloro-phenoxyacetate (2,4-D), hippurate (HA), indoleacetate (IA), indoxyl sulfate (IS) and 3-carboxy-4-methyl-5-propyl-2-furanpropionate (CMPF) and edaravone sulfate. PAH uptake is inhibited by p-chloromercuribenzenesulphonate (PCMBS), diethyl pyrocarbonate (DEPC), indomethacin, sulindac, diclofenac, carprofen, okadaic acid, benzothiazolylcysteine (BTC), S-chlorotrifluoroethylcysteine (CTFC), cysteine S-conjugates S-dichlorovinylcysteine (DCVC), furosemide, steviol, phorbol 12-myristate 13-acetate (PMA), calcium ionophore A23187, benzylpenicillin, bumetamide, losartan, probenecid, phenol red, urate, glutarate and alpha-ketoglutarate (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Solute carrier family 22 member 6
Alternative name(s):
Organic anion transporter 1
Renal organic anion transporter 1
Short name:
ROAT1
Gene namesi
Name:SLC22A6
Synonyms:OAT1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 23CytoplasmicSequence analysisAdd BLAST23
Transmembranei24 – 44HelicalSequence analysisAdd BLAST21
Topological domaini45 – 135ExtracellularSequence analysisAdd BLAST91
Transmembranei136 – 156HelicalSequence analysisAdd BLAST21
Topological domaini157 – 164CytoplasmicSequence analysis8
Transmembranei165 – 185HelicalSequence analysisAdd BLAST21
Topological domaini186 – 195ExtracellularSequence analysis10
Transmembranei196 – 216HelicalSequence analysisAdd BLAST21
Topological domaini217 – 224CytoplasmicSequence analysis8
Transmembranei225 – 245HelicalSequence analysisAdd BLAST21
Topological domaini246 – 248ExtracellularSequence analysis3
Transmembranei249 – 269HelicalSequence analysisAdd BLAST21
Topological domaini270 – 337CytoplasmicSequence analysisAdd BLAST68
Transmembranei338 – 358HelicalSequence analysisAdd BLAST21
Topological domaini359 – 368ExtracellularSequence analysis10
Transmembranei369 – 389HelicalSequence analysisAdd BLAST21
Topological domaini390 – 395CytoplasmicSequence analysis6
Transmembranei396 – 416HelicalSequence analysisAdd BLAST21
Topological domaini417 – 425ExtracellularSequence analysis9
Transmembranei426 – 446HelicalSequence analysisAdd BLAST21
Topological domaini447 – 456CytoplasmicSequence analysis10
Transmembranei457 – 477HelicalSequence analysisAdd BLAST21
Topological domaini478 – 484ExtracellularSequence analysis7
Transmembranei485 – 505HelicalSequence analysisAdd BLAST21
Topological domaini506 – 549CytoplasmicSequence analysisAdd BLAST44

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003241651 – 549Solute carrier family 22 member 6Add BLAST549

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi56N-linked (GlcNAc...)Sequence analysis1
Glycosylationi92N-linked (GlcNAc...)Sequence analysis1
Glycosylationi113N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

Glycosylated. Glycosylation is necessary for proper targeting of the transporter to the plasma membrane (By similarity).By similarity

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ864Z3.
PRIDEiQ864Z3.

Interactioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000018132.

Structurei

3D structure databases

ProteinModelPortaliQ864Z3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

Multiple cysteine residues are necessary for proper targeting to the plasma membrane.By similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0255. Eukaryota.
COG0477. LUCA.
HOGENOMiHOG000234569.
HOVERGENiHBG108433.
InParanoidiQ864Z3.
KOiK08203.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR020846. MFS_dom.
IPR005828. MFS_sugar_transport-like.
IPR004749. Orgcat_transp/SVOP.
[Graphical view]
PfamiPF00083. Sugar_tr. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
TIGRFAMsiTIGR00898. 2A0119. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q864Z3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAFNDLLLQL GGVGRFQKIQ VTLVILPLIL LASHNTLQNF TAAIPTHHCR
60 70 80 90 100
PPADTNLSED GDLEAWLPRD GQGRPESCLL FTSPQRGPPF PNGTETNGTG
110 120 130 140 150
ATEPCPHGWI YDNSTFPSTI VTEWDLVCSH RALRQLAQSL YMMGVLLGAM
160 170 180 190 200
TFGCLADRLG RRKVLIFNYL QTAVSGTCAA FAPNFPAYCA FRFLSGMSTA
210 220 230 240 250
GVVLNCMTLN VEWMPIHTRA YVGTLTGYVY SLGQFLLAGM AYAVPHWRYL
260 270 280 290 300
QLLVSAPFFA FFIYSWFFIE SARWYASSGR LDLTLRNLQR VAWINGKQEE
310 320 330 340 350
GANLSMEALQ ASLKKELTTG KSQASALELI RCPALRRLFL CLSMLWFATS
360 370 380 390 400
FAYYGLVMDL QGFGVSIYLI QVIFGAVDLP AKLVSFLVIN NVGRRPAQMA
410 420 430 440 450
SLLLAGICIL INGVVPKDKS IVRTSLAVLG KGCLASSFNC IFLYTGEVYP
460 470 480 490 500
TMIRQTGLGM GSTLARVGSI VSPLVSMTAE LYPSVPLFIY GAVPVAASAA
510 520 530 540
IALLPETLGQ PLPDTVQDVE NRRRGKTRKQ QEELQKQMVP LQASAQVKN
Length:549
Mass (Da):60,184
Last modified:March 18, 2008 - v2
Checksum:i8D4793CC3C408B69
GO
Isoform 2 (identifier: Q864Z3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-130: MAFNDLLLQL...VTEWDLVCSH → MGRGGPSPAS...TAPSLPPSSL

Note: No experimental confirmation available.
Show »
Length:472
Mass (Da):51,133
Checksum:iBD43DF87C6BD8EE0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti162R → Q in CAD71145 (Ref. 1) Curated1
Sequence conflicti162R → Q in AAX46607 (PubMed:16305752).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0321671 – 130MAFND…LVCSH → MGRGGPSPASSSPPPSGDRP FPMAQRPTAQGPQSPVPTAG STTTAPSLPPSSL in isoform 2. 1 PublicationAdd BLAST130

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ549816 mRNA. Translation: CAD71145.1.
BT021760 mRNA. Translation: AAX46607.1.
BC151704 mRNA. Translation: AAI51705.1.
RefSeqiNP_001001143.1. NM_001001143.2.
UniGeneiBt.28372.

Genome annotation databases

GeneIDi407180.
KEGGibta:407180.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ549816 mRNA. Translation: CAD71145.1.
BT021760 mRNA. Translation: AAX46607.1.
BC151704 mRNA. Translation: AAI51705.1.
RefSeqiNP_001001143.1. NM_001001143.2.
UniGeneiBt.28372.

3D structure databases

ProteinModelPortaliQ864Z3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000018132.

Proteomic databases

PaxDbiQ864Z3.
PRIDEiQ864Z3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi407180.
KEGGibta:407180.

Organism-specific databases

CTDi9356.

Phylogenomic databases

eggNOGiKOG0255. Eukaryota.
COG0477. LUCA.
HOGENOMiHOG000234569.
HOVERGENiHBG108433.
InParanoidiQ864Z3.
KOiK08203.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR020846. MFS_dom.
IPR005828. MFS_sugar_transport-like.
IPR004749. Orgcat_transp/SVOP.
[Graphical view]
PfamiPF00083. Sugar_tr. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
TIGRFAMsiTIGR00898. 2A0119. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiS22A6_BOVIN
AccessioniPrimary (citable) accession number: Q864Z3
Secondary accession number(s): A7E342
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: March 18, 2008
Last modified: November 30, 2016
This is version 96 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.