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Q864Z3

- S22A6_BOVIN

UniProt

Q864Z3 - S22A6_BOVIN

Protein

Solute carrier family 22 member 6

Gene

SLC22A6

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 84 (01 Oct 2014)
      Sequence version 2 (18 Mar 2008)
      Previous versions | rss
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    Functioni

    Involved in the renal elimination of endogenous and exogenous organic anions. Functions as organic anion exchanger when the uptake of one molecule of organic anion is coupled with an efflux of one molecule of endogenous dicarboxylic acid (glutarate, ketoglutarate, etc). Mediates the sodium-independent uptake of p-aminohippurate (PAH), 2,3-dimercapto-1-propanesulfonic acid (DMPS), cidofovir, adefovir, 9-(2-phosphonylmethoxyethyl) guanine (PMEG), 9-(2-phosphonylmethoxyethyl) diaminopurine (PMEDAP), ochratoxin (OTA), acyclovir (ACV), 3'-azido-3-'deoxythymidine (AZT), cimetidine (CMD), 2,4-dichloro-phenoxyacetate (2,4-D), hippurate (HA), indoleacetate (IA), indoxyl sulfate (IS) and 3-carboxy-4-methyl-5-propyl-2-furanpropionate (CMPF) and edaravone sulfate. PAH uptake is inhibited by p-chloromercuribenzenesulphonate (PCMBS), diethyl pyrocarbonate (DEPC), indomethacin, sulindac, diclofenac, carprofen, okadaic acid, benzothiazolylcysteine (BTC), S-chlorotrifluoroethylcysteine (CTFC), cysteine S-conjugates S-dichlorovinylcysteine (DCVC), furosemide, steviol, phorbol 12-myristate 13-acetate (PMA), calcium ionophore A23187, benzylpenicillin, bumetamide, losartan, probenecid, phenol red, urate, glutarate and alpha-ketoglutarate By similarity.By similarity

    GO - Molecular functioni

    1. inorganic anion exchanger activity Source: UniProtKB
    2. organic anion transmembrane transporter activity Source: UniProtKB

    GO - Biological processi

    1. alpha-ketoglutarate transport Source: UniProtKB
    2. organic anion transport Source: UniProtKB
    3. renal tubular secretion Source: UniProtKB

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Solute carrier family 22 member 6
    Alternative name(s):
    Organic anion transporter 1
    Renal organic anion transporter 1
    Short name:
    ROAT1
    Gene namesi
    Name:SLC22A6
    Synonyms:OAT1
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Unplaced

    Subcellular locationi

    GO - Cellular componenti

    1. basolateral plasma membrane Source: UniProtKB
    2. integral component of plasma membrane Source: UniProtKB

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 549549Solute carrier family 22 member 6PRO_0000324165Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi56 – 561N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi92 – 921N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi113 – 1131N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    Glycosylated. Glycosylation is necessary for proper targeting of the transporter to the plasma membrane By similarity.By similarity

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PRIDEiQ864Z3.

    Structurei

    3D structure databases

    ProteinModelPortaliQ864Z3.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 2323CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini45 – 13591ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini157 – 1648CytoplasmicSequence Analysis
    Topological domaini186 – 19510ExtracellularSequence Analysis
    Topological domaini217 – 2248CytoplasmicSequence Analysis
    Topological domaini246 – 2483ExtracellularSequence Analysis
    Topological domaini270 – 33768CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini359 – 36810ExtracellularSequence Analysis
    Topological domaini390 – 3956CytoplasmicSequence Analysis
    Topological domaini417 – 4259ExtracellularSequence Analysis
    Topological domaini447 – 45610CytoplasmicSequence Analysis
    Topological domaini478 – 4847ExtracellularSequence Analysis
    Topological domaini506 – 54944CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei24 – 4421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei136 – 15621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei165 – 18521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei196 – 21621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei225 – 24521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei249 – 26921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei338 – 35821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei369 – 38921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei396 – 41621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei426 – 44621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei457 – 47721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei485 – 50521HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domaini

    Multiple cysteine residues are necessary for proper targeting to the plasma membrane.By similarity

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0477.
    HOGENOMiHOG000234569.
    HOVERGENiHBG108433.
    InParanoidiQ864Z3.
    KOiK08203.

    Family and domain databases

    InterProiIPR020846. MFS_dom.
    IPR016196. MFS_dom_general_subst_transpt.
    IPR004749. Orgcat_transp.
    IPR005828. Sub_transporter.
    [Graphical view]
    PfamiPF00083. Sugar_tr. 1 hit.
    [Graphical view]
    SUPFAMiSSF103473. SSF103473. 1 hit.
    TIGRFAMsiTIGR00898. 2A0119. 1 hit.
    PROSITEiPS50850. MFS. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q864Z3-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAFNDLLLQL GGVGRFQKIQ VTLVILPLIL LASHNTLQNF TAAIPTHHCR    50
    PPADTNLSED GDLEAWLPRD GQGRPESCLL FTSPQRGPPF PNGTETNGTG 100
    ATEPCPHGWI YDNSTFPSTI VTEWDLVCSH RALRQLAQSL YMMGVLLGAM 150
    TFGCLADRLG RRKVLIFNYL QTAVSGTCAA FAPNFPAYCA FRFLSGMSTA 200
    GVVLNCMTLN VEWMPIHTRA YVGTLTGYVY SLGQFLLAGM AYAVPHWRYL 250
    QLLVSAPFFA FFIYSWFFIE SARWYASSGR LDLTLRNLQR VAWINGKQEE 300
    GANLSMEALQ ASLKKELTTG KSQASALELI RCPALRRLFL CLSMLWFATS 350
    FAYYGLVMDL QGFGVSIYLI QVIFGAVDLP AKLVSFLVIN NVGRRPAQMA 400
    SLLLAGICIL INGVVPKDKS IVRTSLAVLG KGCLASSFNC IFLYTGEVYP 450
    TMIRQTGLGM GSTLARVGSI VSPLVSMTAE LYPSVPLFIY GAVPVAASAA 500
    IALLPETLGQ PLPDTVQDVE NRRRGKTRKQ QEELQKQMVP LQASAQVKN 549
    Length:549
    Mass (Da):60,184
    Last modified:March 18, 2008 - v2
    Checksum:i8D4793CC3C408B69
    GO
    Isoform 2 (identifier: Q864Z3-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-130: MAFNDLLLQL...VTEWDLVCSH → MGRGGPSPAS...TAPSLPPSSL

    Note: No experimental confirmation available.

    Show »
    Length:472
    Mass (Da):51,133
    Checksum:iBD43DF87C6BD8EE0
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti162 – 1621R → Q in CAD71145. 1 PublicationCurated
    Sequence conflicti162 – 1621R → Q in AAX46607. (PubMed:16305752)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 130130MAFND…LVCSH → MGRGGPSPASSSPPPSGDRP FPMAQRPTAQGPQSPVPTAG STTTAPSLPPSSL in isoform 2. 1 PublicationVSP_032167Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ549816 mRNA. Translation: CAD71145.1.
    BT021760 mRNA. Translation: AAX46607.1.
    BC151704 mRNA. Translation: AAI51705.1.
    RefSeqiNP_001001143.1. NM_001001143.2.
    UniGeneiBt.28372.

    Genome annotation databases

    GeneIDi407180.
    KEGGibta:407180.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ549816 mRNA. Translation: CAD71145.1 .
    BT021760 mRNA. Translation: AAX46607.1 .
    BC151704 mRNA. Translation: AAI51705.1 .
    RefSeqi NP_001001143.1. NM_001001143.2.
    UniGenei Bt.28372.

    3D structure databases

    ProteinModelPortali Q864Z3.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi Q864Z3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 407180.
    KEGGi bta:407180.

    Organism-specific databases

    CTDi 9356.

    Phylogenomic databases

    eggNOGi COG0477.
    HOGENOMi HOG000234569.
    HOVERGENi HBG108433.
    InParanoidi Q864Z3.
    KOi K08203.

    Miscellaneous databases

    NextBioi 20818436.

    Family and domain databases

    InterProi IPR020846. MFS_dom.
    IPR016196. MFS_dom_general_subst_transpt.
    IPR004749. Orgcat_transp.
    IPR005828. Sub_transporter.
    [Graphical view ]
    Pfami PF00083. Sugar_tr. 1 hit.
    [Graphical view ]
    SUPFAMi SSF103473. SSF103473. 1 hit.
    TIGRFAMsi TIGR00898. 2A0119. 1 hit.
    PROSITEi PS50850. MFS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning of the organic anion transporter 1 from bovine kidney."
      Geyer J., Petzinger E.
      Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Tissue: Kidney.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    3. NIH - Mammalian Gene Collection (MGC) project
      Submitted (AUG-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Strain: Hereford.
      Tissue: Kidney.

    Entry informationi

    Entry nameiS22A6_BOVIN
    AccessioniPrimary (citable) accession number: Q864Z3
    Secondary accession number(s): A7E342
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 18, 2008
    Last sequence update: March 18, 2008
    Last modified: October 1, 2014
    This is version 84 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3