SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q864Z3

- S22A6_BOVIN

UniProt

Q864Z3 - S22A6_BOVIN

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Solute carrier family 22 member 6
Gene
SLC22A6, OAT1
Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Involved in the renal elimination of endogenous and exogenous organic anions. Functions as organic anion exchanger when the uptake of one molecule of organic anion is coupled with an efflux of one molecule of endogenous dicarboxylic acid (glutarate, ketoglutarate, etc). Mediates the sodium-independent uptake of p-aminohippurate (PAH), 2,3-dimercapto-1-propanesulfonic acid (DMPS), cidofovir, adefovir, 9-(2-phosphonylmethoxyethyl) guanine (PMEG), 9-(2-phosphonylmethoxyethyl) diaminopurine (PMEDAP), ochratoxin (OTA), acyclovir (ACV), 3'-azido-3-'deoxythymidine (AZT), cimetidine (CMD), 2,4-dichloro-phenoxyacetate (2,4-D), hippurate (HA), indoleacetate (IA), indoxyl sulfate (IS) and 3-carboxy-4-methyl-5-propyl-2-furanpropionate (CMPF) and edaravone sulfate. PAH uptake is inhibited by p-chloromercuribenzenesulphonate (PCMBS), diethyl pyrocarbonate (DEPC), indomethacin, sulindac, diclofenac, carprofen, okadaic acid, benzothiazolylcysteine (BTC), S-chlorotrifluoroethylcysteine (CTFC), cysteine S-conjugates S-dichlorovinylcysteine (DCVC), furosemide, steviol, phorbol 12-myristate 13-acetate (PMA), calcium ionophore A23187, benzylpenicillin, bumetamide, losartan, probenecid, phenol red, urate, glutarate and alpha-ketoglutarate By similarity.

GO - Molecular functioni

  1. inorganic anion exchanger activity Source: UniProtKB
  2. organic anion transmembrane transporter activity Source: UniProtKB

GO - Biological processi

  1. alpha-ketoglutarate transport Source: UniProtKB
  2. organic anion transport Source: UniProtKB
  3. renal tubular secretion Source: UniProtKB
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Solute carrier family 22 member 6
Alternative name(s):
Organic anion transporter 1
Renal organic anion transporter 1
Short name:
ROAT1
Gene namesi
Name:SLC22A6
Synonyms:OAT1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Unplaced

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2323Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei24 – 4421Helical; Reviewed prediction
Add
BLAST
Topological domaini45 – 13591Extracellular Reviewed prediction
Add
BLAST
Transmembranei136 – 15621Helical; Reviewed prediction
Add
BLAST
Topological domaini157 – 1648Cytoplasmic Reviewed prediction
Transmembranei165 – 18521Helical; Reviewed prediction
Add
BLAST
Topological domaini186 – 19510Extracellular Reviewed prediction
Transmembranei196 – 21621Helical; Reviewed prediction
Add
BLAST
Topological domaini217 – 2248Cytoplasmic Reviewed prediction
Transmembranei225 – 24521Helical; Reviewed prediction
Add
BLAST
Topological domaini246 – 2483Extracellular Reviewed prediction
Transmembranei249 – 26921Helical; Reviewed prediction
Add
BLAST
Topological domaini270 – 33768Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei338 – 35821Helical; Reviewed prediction
Add
BLAST
Topological domaini359 – 36810Extracellular Reviewed prediction
Transmembranei369 – 38921Helical; Reviewed prediction
Add
BLAST
Topological domaini390 – 3956Cytoplasmic Reviewed prediction
Transmembranei396 – 41621Helical; Reviewed prediction
Add
BLAST
Topological domaini417 – 4259Extracellular Reviewed prediction
Transmembranei426 – 44621Helical; Reviewed prediction
Add
BLAST
Topological domaini447 – 45610Cytoplasmic Reviewed prediction
Transmembranei457 – 47721Helical; Reviewed prediction
Add
BLAST
Topological domaini478 – 4847Extracellular Reviewed prediction
Transmembranei485 – 50521Helical; Reviewed prediction
Add
BLAST
Topological domaini506 – 54944Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. basolateral plasma membrane Source: UniProtKB
  2. integral component of plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 549549Solute carrier family 22 member 6
PRO_0000324165Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi56 – 561N-linked (GlcNAc...) Reviewed prediction
Glycosylationi92 – 921N-linked (GlcNAc...) Reviewed prediction
Glycosylationi113 – 1131N-linked (GlcNAc...) Reviewed prediction

Post-translational modificationi

Glycosylated. Glycosylation is necessary for proper targeting of the transporter to the plasma membrane By similarity.

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiQ864Z3.

Structurei

3D structure databases

ProteinModelPortaliQ864Z3.

Family & Domainsi

Domaini

Multiple cysteine residues are necessary for proper targeting to the plasma membrane By similarity.

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0477.
HOGENOMiHOG000234569.
HOVERGENiHBG108433.
InParanoidiQ864Z3.
KOiK08203.

Family and domain databases

InterProiIPR020846. MFS_dom.
IPR016196. MFS_dom_general_subst_transpt.
IPR004749. Orgcat_transp.
IPR005828. Sub_transporter.
[Graphical view]
PfamiPF00083. Sugar_tr. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
TIGRFAMsiTIGR00898. 2A0119. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q864Z3-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MAFNDLLLQL GGVGRFQKIQ VTLVILPLIL LASHNTLQNF TAAIPTHHCR    50
PPADTNLSED GDLEAWLPRD GQGRPESCLL FTSPQRGPPF PNGTETNGTG 100
ATEPCPHGWI YDNSTFPSTI VTEWDLVCSH RALRQLAQSL YMMGVLLGAM 150
TFGCLADRLG RRKVLIFNYL QTAVSGTCAA FAPNFPAYCA FRFLSGMSTA 200
GVVLNCMTLN VEWMPIHTRA YVGTLTGYVY SLGQFLLAGM AYAVPHWRYL 250
QLLVSAPFFA FFIYSWFFIE SARWYASSGR LDLTLRNLQR VAWINGKQEE 300
GANLSMEALQ ASLKKELTTG KSQASALELI RCPALRRLFL CLSMLWFATS 350
FAYYGLVMDL QGFGVSIYLI QVIFGAVDLP AKLVSFLVIN NVGRRPAQMA 400
SLLLAGICIL INGVVPKDKS IVRTSLAVLG KGCLASSFNC IFLYTGEVYP 450
TMIRQTGLGM GSTLARVGSI VSPLVSMTAE LYPSVPLFIY GAVPVAASAA 500
IALLPETLGQ PLPDTVQDVE NRRRGKTRKQ QEELQKQMVP LQASAQVKN 549
Length:549
Mass (Da):60,184
Last modified:March 18, 2008 - v2
Checksum:i8D4793CC3C408B69
GO
Isoform 2 (identifier: Q864Z3-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-130: MAFNDLLLQL...VTEWDLVCSH → MGRGGPSPAS...TAPSLPPSSL

Note: No experimental confirmation available.

Show »
Length:472
Mass (Da):51,133
Checksum:iBD43DF87C6BD8EE0
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 130130MAFND…LVCSH → MGRGGPSPASSSPPPSGDRP FPMAQRPTAQGPQSPVPTAG STTTAPSLPPSSL in isoform 2.
VSP_032167Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti162 – 1621R → Q in CAD71145. 1 Publication
Sequence conflicti162 – 1621R → Q in AAX46607. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ549816 mRNA. Translation: CAD71145.1.
BT021760 mRNA. Translation: AAX46607.1.
BC151704 mRNA. Translation: AAI51705.1.
RefSeqiNP_001001143.1. NM_001001143.2.
UniGeneiBt.28372.

Genome annotation databases

GeneIDi407180.
KEGGibta:407180.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ549816 mRNA. Translation: CAD71145.1 .
BT021760 mRNA. Translation: AAX46607.1 .
BC151704 mRNA. Translation: AAI51705.1 .
RefSeqi NP_001001143.1. NM_001001143.2.
UniGenei Bt.28372.

3D structure databases

ProteinModelPortali Q864Z3.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi Q864Z3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 407180.
KEGGi bta:407180.

Organism-specific databases

CTDi 9356.

Phylogenomic databases

eggNOGi COG0477.
HOGENOMi HOG000234569.
HOVERGENi HBG108433.
InParanoidi Q864Z3.
KOi K08203.

Miscellaneous databases

NextBioi 20818436.

Family and domain databases

InterProi IPR020846. MFS_dom.
IPR016196. MFS_dom_general_subst_transpt.
IPR004749. Orgcat_transp.
IPR005828. Sub_transporter.
[Graphical view ]
Pfami PF00083. Sugar_tr. 1 hit.
[Graphical view ]
SUPFAMi SSF103473. SSF103473. 1 hit.
TIGRFAMsi TIGR00898. 2A0119. 1 hit.
PROSITEi PS50850. MFS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of the organic anion transporter 1 from bovine kidney."
    Geyer J., Petzinger E.
    Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Kidney.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  3. NIH - Mammalian Gene Collection (MGC) project
    Submitted (AUG-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: Hereford.
    Tissue: Kidney.

Entry informationi

Entry nameiS22A6_BOVIN
AccessioniPrimary (citable) accession number: Q864Z3
Secondary accession number(s): A7E342
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: March 18, 2008
Last modified: April 16, 2014
This is version 83 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi