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Q864Z3 (S22A6_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Solute carrier family 22 member 6
Alternative name(s):
Organic anion transporter 1
Renal organic anion transporter 1
Short name=ROAT1
Gene names
Name:SLC22A6
Synonyms:OAT1
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length549 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Involved in the renal elimination of endogenous and exogenous organic anions. Functions as organic anion exchanger when the uptake of one molecule of organic anion is coupled with an efflux of one molecule of endogenous dicarboxylic acid (glutarate, ketoglutarate, etc). Mediates the sodium-independent uptake of p-aminohippurate (PAH), 2,3-dimercapto-1-propanesulfonic acid (DMPS), cidofovir, adefovir, 9-(2-phosphonylmethoxyethyl) guanine (PMEG), 9-(2-phosphonylmethoxyethyl) diaminopurine (PMEDAP), ochratoxin (OTA), acyclovir (ACV), 3'-azido-3-'deoxythymidine (AZT), cimetidine (CMD), 2,4-dichloro-phenoxyacetate (2,4-D), hippurate (HA), indoleacetate (IA), indoxyl sulfate (IS) and 3-carboxy-4-methyl-5-propyl-2-furanpropionate (CMPF) and edaravone sulfate. PAH uptake is inhibited by p-chloromercuribenzenesulphonate (PCMBS), diethyl pyrocarbonate (DEPC), indomethacin, sulindac, diclofenac, carprofen, okadaic acid, benzothiazolylcysteine (BTC), S-chlorotrifluoroethylcysteine (CTFC), cysteine S-conjugates S-dichlorovinylcysteine (DCVC), furosemide, steviol, phorbol 12-myristate 13-acetate (PMA), calcium ionophore A23187, benzylpenicillin, bumetamide, losartan, probenecid, phenol red, urate, glutarate and alpha-ketoglutarate By similarity.

Subcellular location

Cell membrane; Multi-pass membrane protein Potential.

Domain

Multiple cysteine residues are necessary for proper targeting to the plasma membrane By similarity.

Post-translational modification

Glycosylated. Glycosylation is necessary for proper targeting of the transporter to the plasma membrane By similarity.

Sequence similarities

Belongs to the major facilitator (TC 2.A.1) superfamily. Organic cation transporter (TC 2.A.1.19) family. [View classification]

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q864Z3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q864Z3-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-130: MAFNDLLLQL...VTEWDLVCSH → MGRGGPSPAS...TAPSLPPSSL
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 549549Solute carrier family 22 member 6
PRO_0000324165

Regions

Topological domain1 – 2323Cytoplasmic Potential
Transmembrane24 – 4421Helical; Potential
Topological domain45 – 13591Extracellular Potential
Transmembrane136 – 15621Helical; Potential
Topological domain157 – 1648Cytoplasmic Potential
Transmembrane165 – 18521Helical; Potential
Topological domain186 – 19510Extracellular Potential
Transmembrane196 – 21621Helical; Potential
Topological domain217 – 2248Cytoplasmic Potential
Transmembrane225 – 24521Helical; Potential
Topological domain246 – 2483Extracellular Potential
Transmembrane249 – 26921Helical; Potential
Topological domain270 – 33768Cytoplasmic Potential
Transmembrane338 – 35821Helical; Potential
Topological domain359 – 36810Extracellular Potential
Transmembrane369 – 38921Helical; Potential
Topological domain390 – 3956Cytoplasmic Potential
Transmembrane396 – 41621Helical; Potential
Topological domain417 – 4259Extracellular Potential
Transmembrane426 – 44621Helical; Potential
Topological domain447 – 45610Cytoplasmic Potential
Transmembrane457 – 47721Helical; Potential
Topological domain478 – 4847Extracellular Potential
Transmembrane485 – 50521Helical; Potential
Topological domain506 – 54944Cytoplasmic Potential

Amino acid modifications

Glycosylation561N-linked (GlcNAc...) Potential
Glycosylation921N-linked (GlcNAc...) Potential
Glycosylation1131N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence1 – 130130MAFND…LVCSH → MGRGGPSPASSSPPPSGDRP FPMAQRPTAQGPQSPVPTAG STTTAPSLPPSSL in isoform 2.
VSP_032167

Experimental info

Sequence conflict1621R → Q in CAD71145. Ref.1
Sequence conflict1621R → Q in AAX46607. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 18, 2008. Version 2.
Checksum: 8D4793CC3C408B69

FASTA54960,184
        10         20         30         40         50         60 
MAFNDLLLQL GGVGRFQKIQ VTLVILPLIL LASHNTLQNF TAAIPTHHCR PPADTNLSED 

        70         80         90        100        110        120 
GDLEAWLPRD GQGRPESCLL FTSPQRGPPF PNGTETNGTG ATEPCPHGWI YDNSTFPSTI 

       130        140        150        160        170        180 
VTEWDLVCSH RALRQLAQSL YMMGVLLGAM TFGCLADRLG RRKVLIFNYL QTAVSGTCAA 

       190        200        210        220        230        240 
FAPNFPAYCA FRFLSGMSTA GVVLNCMTLN VEWMPIHTRA YVGTLTGYVY SLGQFLLAGM 

       250        260        270        280        290        300 
AYAVPHWRYL QLLVSAPFFA FFIYSWFFIE SARWYASSGR LDLTLRNLQR VAWINGKQEE 

       310        320        330        340        350        360 
GANLSMEALQ ASLKKELTTG KSQASALELI RCPALRRLFL CLSMLWFATS FAYYGLVMDL 

       370        380        390        400        410        420 
QGFGVSIYLI QVIFGAVDLP AKLVSFLVIN NVGRRPAQMA SLLLAGICIL INGVVPKDKS 

       430        440        450        460        470        480 
IVRTSLAVLG KGCLASSFNC IFLYTGEVYP TMIRQTGLGM GSTLARVGSI VSPLVSMTAE 

       490        500        510        520        530        540 
LYPSVPLFIY GAVPVAASAA IALLPETLGQ PLPDTVQDVE NRRRGKTRKQ QEELQKQMVP 


LQASAQVKN 

« Hide

Isoform 2 [UniParc].

Checksum: BD43DF87C6BD8EE0
Show »

FASTA47251,133

References

« Hide 'large scale' references
[1]"Cloning of the organic anion transporter 1 from bovine kidney."
Geyer J., Petzinger E.
Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Kidney.
[2]"Characterization of 954 bovine full-CDS cDNA sequences."
Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L.
BMC Genomics 6:166-166(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[3]NIH - Mammalian Gene Collection (MGC) project
Submitted (AUG-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: Hereford.
Tissue: Kidney.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ549816 mRNA. Translation: CAD71145.1.
BT021760 mRNA. Translation: AAX46607.1.
BC151704 mRNA. Translation: AAI51705.1.
RefSeqNP_001001143.1. NM_001001143.2.
UniGeneBt.28372.

3D structure databases

ProteinModelPortalQ864Z3.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ864Z3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID407180.
KEGGbta:407180.

Organism-specific databases

CTD9356.

Phylogenomic databases

eggNOGCOG0477.
HOGENOMHOG000234569.
HOVERGENHBG108433.
InParanoidQ864Z3.
KOK08203.

Family and domain databases

InterProIPR020846. MFS_dom.
IPR016196. MFS_dom_general_subst_transpt.
IPR004749. Orgcat_transp.
IPR005828. Sub_transporter.
[Graphical view]
PfamPF00083. Sugar_tr. 1 hit.
[Graphical view]
SUPFAMSSF103473. SSF103473. 1 hit.
TIGRFAMsTIGR00898. 2A0119. 1 hit.
PROSITEPS50850. MFS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20818436.

Entry information

Entry nameS22A6_BOVIN
AccessionPrimary (citable) accession number: Q864Z3
Secondary accession number(s): A7E342
Entry history
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: March 18, 2008
Last modified: April 16, 2014
This is version 83 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families