Reviewed, UniProtKB/Swiss-Prot Q864U6 (B3GL1_PIG)

Names and origin

Protein names

Recommended name:
    UDP-GalNAc:beta-1,3-N-acetylgalactosaminyltransferase 1
      Short name=Beta-1,3-GalNAc-T1
    EC=2.4.1.79
Alternative name(s):
    Beta-1,3-galactosyltransferase 3
      Short name=Beta-1,3-GalTase 3
      Short name=Beta3Gal-T3
      Short name=Beta3GalT3
      Short name=b3Gal-T3
    Beta-3-Gx-T3
    Galactosylgalactosylglucosylceramide beta-D-acetyl-galactosaminyltransferase
    UDP-N-acetylgalactosamine:globotriaosylceramide beta-1,3-N-acetylgalactosaminyltransferase
    Globoside synthase

Gene names

Name:	B3GALNT1
Synonyms:	B3GALT3

Organism

Sus scrofa (Pig)

Taxonomic identifier

9823 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus

Protein attributes

Sequence length	331 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Transfers N-acetylgalactosamine onto globotriaosylceramide By similarity.
Catalytic activity	UDP-N-acetyl-D-galactosamine + alpha-D-galactosyl-(1->4)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide = UDP + N-acetyl-beta-D-galactosaminyl-(1->3)-alpha-D-galactosyl-(1->4)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide.
Cofactor	Magnesium By similarity.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein By similarity.
Sequence similarities	Belongs to the glycosyltransferase 31 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Ligand	Magnesium
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein Phosphoprotein
Gene Ontology (GO)
Biological process	protein amino acid glycosylation Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	galactosylgalactosylglucosylceramide beta-D-acetylgalactosaminyltransferase activity Inferred from electronic annotation. Source: EC galactosyltransferase activity Inferred from electronic annotation. Source: InterPro magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 331	331	UDP-GalNAc:beta-1,3-N-acetylgalactosaminyltransferase 1		PRO_0000219156
Regions
	Topological domain	1 – 20	20	Cytoplasmic Potential
	Transmembrane	21 – 43	23	Signal-anchor for type II membrane protein Potential
	Topological domain	44 – 331	288	Lumenal Potential
Amino acid modifications
	Modified residue	140	1	Phosphotyrosine By similarity
	Modified residue	152	1	Phosphotyrosine By similarity
	Glycosylation	72	1	N-linked (GlcNAc...) Potential
	Glycosylation	154	1	N-linked (GlcNAc...) Potential
	Glycosylation	198	1	N-linked (GlcNAc...) Potential
	Glycosylation	212	1	N-linked (GlcNAc...) Potential
	Glycosylation	326	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q864U6-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 3686FB95DA581CE3	FASTA	331	39,437
	10 20 30 40 50 60 MAPALPITLP SKMSLRSLKW SLLLLSLLSF LVMWYLSLPH YNVIERVNWM YFYEYEPIYR 70 80 90 100 110 120 QDFHFTLREH SNCSHQNPFL VILVTSHPAD VKARQAIRVT WGEKKSWWGY EVLTFFLLGQ 130 140 150 160 170 180 QAEREDKVLA LSLEDEHLLY GDIIRQDFLD TYNNLTLKTI MAFRWVTEFC PNARYIMKTD 190 200 210 220 230 240 TDVFINTGNL VKYLLNLNHS EKFFTGYPLI DNYSYRGFYQ KTHISYQEYP FKVFPPYCSG 250 260 270 280 290 300 LGYIMSRDLV PRIYEMMSHV KPIKFEDVYV GICLNLLKVD IHIPEDTNLF FLYRIHLDVC 310 320 330 QLRRVIAAHG FSSKEIITFW QVMLRNTTCH Y

References

[1]	Python P., Joerg H., Neuenschwander S., Stranzinger G., Voegeli P. Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Small intestine.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY253340 mRNA. Translation: AAO92025.1.
RefSeq	NP_999516.1.
UniGene	Ssc.13482
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT31. Glycosyltransferase Family 31.
Genome annotation databases
GeneID	397634.
KEGG	ssc:397634.
Organism-specific databases
CTD	397634.
Phylogenomic databases
HOVERGEN	Q864U6.
Enzyme and pathway databases
BRENDA	2.4.1.79. 249.
Family and domain databases
InterPro	IPR002659. Glyco_trans_31. [Graphical view]
PANTHER	PTHR11214. Glyco_trans_31. 1 hit.
Pfam	PF01762. Galactosyl_T. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B3GL1_PIG

Accession

Primary (citable) accession number: Q864U6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 5, 2005
Last sequence update:	June 1, 2003
Last modified:	February 9, 2010
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)