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Q864S8

- BRCA2_FELCA

UniProt

Q864S8 - BRCA2_FELCA

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Protein

Breast cancer type 2 susceptibility protein homolog

Gene
BRCA2, FANCD1
Organism
Felis catus (Cat) (Felis silvestris catus)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Involved in double-strand break repair and/or homologous recombination. Binds RAD51 and potentiates recombinational DNA repair by promoting assembly of RAD51 onto single-stranded DNA (ssDNA). Acts by targeting RAD51 to ssDNA over double-stranded DNA, enabling RAD51 to displace replication protein-A (RPA) from ssDNA and stabilizing RAD51-ssDNA filaments by blocking ATP hydrolysis. Part of a PALB2-scaffolded HR complex containing RAD51C and which is thought to play a role in DNA repair by HR. May participate in S phase checkpoint activation. Binds selectively to ssDNA, and to ssDNA in tailed duplexes and replication fork structures. May play a role in the extension step after strand invasion at replication-dependent DNA double-strand breaks; together with PALB2 is involved in both POLH localization at collapsed replication forks and DNA polymerization activity. In concert with NPM1, regulates centrosome duplication By similarity.

GO - Molecular functioni

  1. gamma-tubulin binding Source: UniProtKB
  2. single-stranded DNA binding Source: InterPro

GO - Biological processi

  1. centrosome duplication Source: UniProtKB
  2. cytokinesis Source: UniProtKB
  3. double-strand break repair Source: UniProtKB
  4. double-strand break repair via homologous recombination Source: InterPro
  5. negative regulation of mammary gland epithelial cell proliferation Source: UniProtKB
  6. nucleotide-excision repair Source: UniProtKB
  7. positive regulation of transcription, DNA-templated Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Breast cancer type 2 susceptibility protein homolog
Alternative name(s):
Fanconi anemia group D1 protein homolog
Gene namesi
Name:BRCA2
Synonyms:FANCD1
OrganismiFelis catus (Cat) (Felis silvestris catus)
Taxonomic identifieri9685 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraFeliformiaFelidaeFelinaeFelis
ProteomesiUP000011712: Unplaced

Subcellular locationi

Nucleus By similarity. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity

GO - Cellular componenti

  1. BRCA2-MAGE-D1 complex Source: UniProtKB
  2. centrosome Source: UniProtKB
  3. nucleus Source: UniProtKB
  4. protein complex Source: UniProtKB
  5. secretory granule Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

Pathology & Biotechi

Keywords - Diseasei

Tumor suppressor

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 33723372Breast cancer type 2 susceptibility protein homologPRO_0000227014Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei3236 – 32361Phosphoserine; by CDK1 and CDK2 By similarity
Modified residuei3331 – 33311Phosphothreonine; by CHEK1 and CHEK2 By similarity

Post-translational modificationi

Phosphorylation by CHEK1 and CHEK2 regulates interaction with RAD51. Phosphorylation at Ser-3236 by CDK1 and CDK2 is low in S phase when recombination is active, but increases as cells progress towards mitosis; this phosphorylation prevents homologous recombination-dependent repair during S phase and G2 by inhibiting RAD51 binding By similarity.
Ubiquitinated in the absence of DNA damage; this does not lead to proteasomal degradation. In contrast, ubiquitination in response to DNA damage leads to proteasomal degradation By similarity.

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Interactioni

Subunit structurei

Monomer and dimer. Interacts with RAD51; regulates RAD51 recruitment and function at sites of DNA repair. Interacts with DSS1, WDR16, USP11, DMC1, ROCK2 and NPM1. Interacts with both nonubiquitinated and monoubiquitinated FANCD2; this complex also includes XRCC3 and phosphorylated FANCG. Part of a BRCA complex containing BRCA1, BRCA2 and PALB2. Interacts directly with PALB2 which may serve as a scaffold for a HR complex containing PALB2, BRCA2, RAD51C, RAD51 and XRCC3. Interacts with BRCA1 only in the presence of PALB2 which serves as the bridging protein. Interacts with POLH; the interaction is direct By similarity.

Protein-protein interaction databases

IntActiQ864S8. 1 interaction.
MINTiMINT-8176579.

Structurei

3D structure databases

ProteinModelPortaliQ864S8.
SMRiQ864S8. Positions 1520-1552, 2423-3128.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati994 – 102835BRCA2 1Add
BLAST
Repeati1208 – 124235BRCA2 2Add
BLAST
Repeati1417 – 145135BRCA2 3Add
BLAST
Repeati1518 – 155235BRCA2 4Add
BLAST
Repeati1663 – 169735BRCA2 5Add
BLAST
Repeati1925 – 195935BRCA2 6Add
BLAST
Repeati1995 – 202935BRCA2 7Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 4040Interaction with PALB2 By similarityAdd
BLAST
Regioni630 – 992363Interaction with NPM1 By similarityAdd
BLAST

Sequence similaritiesi

Contains 7 BRCA2 repeats.

Keywords - Domaini

Repeat

Phylogenomic databases

HOVERGENiHBG050731.
KOiK08775.

Family and domain databases

Gene3Di2.40.50.140. 4 hits.
InterProiIPR015525. BRCA2.
IPR015252. BRCA2_hlx.
IPR015187. BRCA2_OB_1.
IPR015188. BRCA2_OB_3.
IPR002093. BRCA2_repeat.
IPR012340. NA-bd_OB-fold.
IPR015205. Tower.
[Graphical view]
PANTHERiPTHR11289. PTHR11289. 1 hit.
PfamiPF09169. BRCA-2_helical. 1 hit.
PF09103. BRCA-2_OB1. 1 hit.
PF09104. BRCA-2_OB3. 1 hit.
PF00634. BRCA2. 7 hits.
PF09121. Tower. 1 hit.
[Graphical view]
PIRSFiPIRSF002397. BRCA2. 1 hit.
SUPFAMiSSF50249. SSF50249. 4 hits.
SSF81872. SSF81872. 1 hit.
PROSITEiPS50138. BRCA2_REPEAT. 7 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q864S8-1 [UniParc]FASTAAdd to Basket

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MPIGCKERPT FFEIFRTRCN KADLGPISLN WFEELCLEAP PYNSEPTEES     50
GYKISYEPNL FKTPQRKPCH QLASTPIIFK EQGLIPPIYQ QSPLKELGKD 100
ITNSKHRSCC TMKSKMDQTN DVTSPPLNSC LSESPLLRST HVTPQREKSV 150
VCGSLFHTPK LTKGQTPKRI SESLGAEVDP DMSWSSSLAT PPTLSSTVLI 200
VRDEEASAAV FPNDTTAIFK SYFCNHDESL KKNDRFIPSG PDSENKSQRE 250
AKSQGLGKMV GNSCDKVNSC KDPFGNSTLN VLEDGVRERV ADVSEEDSFP 300
LCVPKCKTRN LQKIKTSKTR KNIFNETTDE CKEAKKQMKE NKHSFVSEME 350
ANASDPLDSN VTNQKPFGNG SDKISKEVVL SSASESCHLT LSGLNGTHME 400
KLPLLCISSC DQNNSEKDLI TTEKECTNFI ILEDSLPQIS GVPKCTEKIL 450
NEEIVVNKID EGQCLESHED SILAVKQAVF ETSLIASPLQ GIRKSIFRIR 500
ESPEETFSAV FSNNITDPNF KEEHEASESV LEKHSICSQK EDSLSTSSLD 550
NGSWPATIKH TSVALKNSGL ISTLKKKTKK FIYVVNDETS YQGLKTQKDQ 600
QSGLMNYSAQ FEANVLEGPL TFANADSGLL HSSVKKTCLQ NDSKEPILSL 650
TNSFGTLLRK VSNKGSSSPN NKIISQDLDY KEAKIKKEKL QSFISTETNC 700
LSSLQEKHCE DDTKSQRVAD RKEEILPAVS QPSVPYSEVE DSGIHFQTLK 750
SFSSDPDKSS QLTPHPRDPP SNPVGLSRGR ESYEVSETLK CKNHEAGFEL 800
TKTMENSQEI HVLNEHAKKA KLLSTEKYVT EASPSMKVPF NQNAHLTIIQ 850
KDQKETTLIS KITMNPNSEE LFPDGDNFVF KITKERNVPV LGSIKELQDS 900
DLCCVKEPVL ENSTMVVYTD MDDKQAAKVS ITKGFDSSNI DDLTEKDRNS 950
IKQQLRMTLD QDSKSDITLD SDMKSNGNND YMDNWARLSD PILNHNFGNG 1000
FRTASNKEIK LSEHNIKKSK MLFKDIEERY PTNLACIEIV NTPLESQEKL 1050
SKPHILDPQS INTVSGCVQS SAYVSDSENR HTTPPTLSLK RDFDSNHNLT 1100
PSQKAEITEL STILEESGSQ FEFTQFRKPS HLKQKNPCEM PEKHLTISNT 1150
TPEEQKDGHL RLTINALSIS QGDSSKKFEG IIGGKQKLAC LSKTSCNKSA 1200
SGHLTGKNEV EFRGFYSARG TKLNVCSEAL QKAKKLFSDL ENISEETSVE 1250
VDRSFSSSKC NGSVSMFKKE NCNNEKKLNE KNNKYRLILQ NNIEMTTGIF 1300
VAEDTEGYKR NIENKANKYT DASRNVYNFR EADGSDSSKN DTVYIHKEEN 1350
GLPYIDQHDI DLKLSSQFIK EGNTQIKEGL SDLTCLEVVK AEETLHVNTS 1400
NKEHLTANTM GRITKDFDIF DVSFQTASGK NIRVSRASLN KVTNLLDQKC 1450
TEEELNNFAD SLNSELLSGI DINKADISHH GEMEILKKRQ MKESDLTGTE 1500
NKSLTLQQRP EYEIKKIKEP TILGFHTASG KRIEIAKESL DKVKNLFDEQ 1550
EQDKSEMTNF SHRGTKMSKG REECEGGLRL ACKTIEITPA SKEEEMQKPL 1600
EKNLVSNEIV VVPRLLSDNL YKQTENLKIP NRASLKVKVH ENTGKETAKK 1650
PTTCTNQSTY SATENSALSF YTGHGRKISV SQSSILEVKK WLRGGELDDQ 1700
PEKTVYNISE YLPKSKVDNS GIEPVVRNVG ERENTSVSEI MFTVREADTD 1750
PQSVNEDICV QRLVTNFSCK KENTAIKVTV SDSNNFDSTQ KLNSDSNDAV 1800
PVYTTASSER VLVAHETKVA EGFTENCSMA IKQTTKSKPG KIVAGYRKAP 1850
DDSEDTICPN SLDGAECSSP SHKDFAETQS EQTPQLNQSI SGFKKRSEIP 1900
PHQINLKTSD ICKLSTGKRL QSISYTNACG IFSTASGKCV QVSDAALQKA 1950
RQVFSKVEDS AKQPFSKVSF KHNEDHSDKF TREENTMIHT PQNLLSSAFS 2000
GFSTASGKQV PVSESALCKV KGILEEFDVM RTECGPQRSP TSRQDVSKMP 2050
PPSCVENKTP KHSVNSKLEK AYNKEFKLSS NSKIENGSSE NHSVQVSPYP 2100
SQFKQDKQLI QGNKASLVEN IHLLEKEQAL PKNIKWKLET EAFPNLPLKT 2150
DTAIHSTDSK DPENYFETET VEIAKAFMED GELTDADLLS HARHFLPTCQ 2200
HSEETLVSNS RRGKRRGVLV SVGEPPIKRN LLNEFDRIIK NQEKSLKASK 2250
STPDGIIKDR SLFMHHISLE PVTCGPFSTT KKRQEIQNPN FTAPGQKFLS 2300
KSHFYEHLAL EKSSSNVSIS GQPFCTVPAT RSEKRGHSIT PSKPVKVFVP 2350
PFKTKSRFLQ DEQHISKNTH VEENKQKPNN IDEHSSGDSK NNINNSEIHQ 2400
LNKNNSSQAA TMVFTKCEKE PLDLIASLQN ARDIQDMRIR EKRKQHIFPQ 2450
PGSLFLAKTS TVPRISLRVA VEGRVPSACS HKQLYMYGVS KHCVKINSKN 2500
AESFQFHTQD YFGKEVQWAK EGIQLADGGW LIPSNDGKAG KEEFYRALCD 2550
TPGVDPNLIS RIWVYNHYRW IIWKLAAMEF AFPKEFANRC LSPERVLLQL 2600
KYRYDMEIDR SKRSAIKKIM ERDDTAAKTL VLCISETISS STDLSETSGS 2650
KTSGVGTKNV GIVELTDGWY AIKAQLDPPL LALVKKGRLT VGHKIIIHGA 2700
ELAGSPDACT PLEAPESLIL KISANSTRPA CWYAKLGFFP DPRPFPLPLS 2750
SLFSDGGNVG CVDVVIQRTY PIQWMEKTPS GLCIFRNERE EEREATKYAE 2800
AQQKKLEVLF NKIQAEFEKH DENITKRCVP LRALTRQQVC ALQDGAELYE 2850
AVKNAPDPAS LEAYFSEEQI RALNNHRQML NDKKQAQIQL EFRKAMESAE 2900
QGEQMLPRDV TTVWKMRIIS YGKKEKDSVT LSIWRPSSDL YSLLTEGKRY 2950
RIYHLATSQS KSKSERAHIQ LTATKKTQYQ QLPASDELLF QVYQPREPLY 3000
FNKLLDPDFQ PPCSEVDLIG FVVSVVKKIG FAPLVYLSDE CHNLLAIKVW 3050
TDLNEDIVKP HTLIAASNLQ WRPESKSGIP TLFAGDFSRF SASPKEGHFQ 3100
ETFHKMKNTI ENVETFCNDA ENKLVHILNA NSPKVSTPMK DYASEPHTIQ 3150
TVLGLGNKLS MSSPNSEMNY QSPLSLCKPK AKSVPTPGSA QMTSKSCYKG 3200
ERELDDPKTC KKRKALDFLS RLPLPPPVSP ICTFVSPAAQ KAFQPPRSCG 3250
TKYETPIKKR ELNSPQMTPL KFNDTSLVES DSIADEELAL INTQALLSGL 3300
AGEDQLMSLN DSPRTAPTSS KDYVRPKSYP TAPGIRDCEN PQASTEGGEP 3350
DVQDTDTVKR SSMRLQRRQQ QT 3372
Length:3,372
Mass (Da):377,345
Last modified:January 4, 2005 - v2
Checksum:i37F23DA23CA94665
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB107955 mRNA. Translation: BAC75821.2.
RefSeqiNP_001009858.1. NM_001009858.1.

Genome annotation databases

GeneIDi493878.
KEGGifca:493878.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB107955 mRNA. Translation: BAC75821.2 .
RefSeqi NP_001009858.1. NM_001009858.1.

3D structure databases

ProteinModelPortali Q864S8.
SMRi Q864S8. Positions 1520-1552, 2423-3128.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q864S8. 1 interaction.
MINTi MINT-8176579.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 493878.
KEGGi fca:493878.

Organism-specific databases

CTDi 675.

Phylogenomic databases

HOVERGENi HBG050731.
KOi K08775.

Family and domain databases

Gene3Di 2.40.50.140. 4 hits.
InterProi IPR015525. BRCA2.
IPR015252. BRCA2_hlx.
IPR015187. BRCA2_OB_1.
IPR015188. BRCA2_OB_3.
IPR002093. BRCA2_repeat.
IPR012340. NA-bd_OB-fold.
IPR015205. Tower.
[Graphical view ]
PANTHERi PTHR11289. PTHR11289. 1 hit.
Pfami PF09169. BRCA-2_helical. 1 hit.
PF09103. BRCA-2_OB1. 1 hit.
PF09104. BRCA-2_OB3. 1 hit.
PF00634. BRCA2. 7 hits.
PF09121. Tower. 1 hit.
[Graphical view ]
PIRSFi PIRSF002397. BRCA2. 1 hit.
SUPFAMi SSF50249. SSF50249. 4 hits.
SSF81872. SSF81872. 1 hit.
PROSITEi PS50138. BRCA2_REPEAT. 7 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Properties of the tumor suppressor gene brca2 in the cat."
    Oonuma T., Morimatsu M., Ochiai K., Syuto B.
    J. Vet. Med. Sci. 65:1123-1126(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiBRCA2_FELCA
AccessioniPrimary (citable) accession number: Q864S8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: January 4, 2005
Last modified: May 14, 2014
This is version 64 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi