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Q864S8

- BRCA2_FELCA

UniProt

Q864S8 - BRCA2_FELCA

Protein

Breast cancer type 2 susceptibility protein homolog

Gene

BRCA2

Organism
Felis catus (Cat) (Felis silvestris catus)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 65 (01 Oct 2014)
      Sequence version 2 (04 Jan 2005)
      Previous versions | rss
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    Functioni

    Involved in double-strand break repair and/or homologous recombination. Binds RAD51 and potentiates recombinational DNA repair by promoting assembly of RAD51 onto single-stranded DNA (ssDNA). Acts by targeting RAD51 to ssDNA over double-stranded DNA, enabling RAD51 to displace replication protein-A (RPA) from ssDNA and stabilizing RAD51-ssDNA filaments by blocking ATP hydrolysis. Part of a PALB2-scaffolded HR complex containing RAD51C and which is thought to play a role in DNA repair by HR. May participate in S phase checkpoint activation. Binds selectively to ssDNA, and to ssDNA in tailed duplexes and replication fork structures. May play a role in the extension step after strand invasion at replication-dependent DNA double-strand breaks; together with PALB2 is involved in both POLH localization at collapsed replication forks and DNA polymerization activity. In concert with NPM1, regulates centrosome duplication By similarity.By similarity

    GO - Molecular functioni

    1. gamma-tubulin binding Source: UniProtKB
    2. single-stranded DNA binding Source: InterPro

    GO - Biological processi

    1. centrosome duplication Source: UniProtKB
    2. cytokinesis Source: UniProtKB
    3. double-strand break repair Source: UniProtKB
    4. double-strand break repair via homologous recombination Source: InterPro
    5. negative regulation of mammary gland epithelial cell proliferation Source: UniProtKB
    6. nucleotide-excision repair Source: UniProtKB
    7. positive regulation of transcription, DNA-templated Source: UniProtKB

    Keywords - Biological processi

    DNA damage, DNA recombination, DNA repair

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Breast cancer type 2 susceptibility protein homolog
    Alternative name(s):
    Fanconi anemia group D1 protein homolog
    Gene namesi
    Name:BRCA2
    Synonyms:FANCD1
    OrganismiFelis catus (Cat) (Felis silvestris catus)
    Taxonomic identifieri9685 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraFeliformiaFelidaeFelinaeFelis
    ProteomesiUP000011712: Unplaced

    Subcellular locationi

    Nucleus By similarity. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity

    GO - Cellular componenti

    1. BRCA2-MAGE-D1 complex Source: UniProtKB
    2. centrosome Source: UniProtKB
    3. nucleus Source: UniProtKB
    4. protein complex Source: UniProtKB
    5. secretory granule Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton, Nucleus

    Pathology & Biotechi

    Keywords - Diseasei

    Tumor suppressor

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 33723372Breast cancer type 2 susceptibility protein homologPRO_0000227014Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei3236 – 32361Phosphoserine; by CDK1 and CDK2By similarity
    Modified residuei3331 – 33311Phosphothreonine; by CHEK1 and CHEK2By similarity

    Post-translational modificationi

    Phosphorylation by CHEK1 and CHEK2 regulates interaction with RAD51. Phosphorylation at Ser-3236 by CDK1 and CDK2 is low in S phase when recombination is active, but increases as cells progress towards mitosis; this phosphorylation prevents homologous recombination-dependent repair during S phase and G2 by inhibiting RAD51 binding By similarity.By similarity
    Ubiquitinated in the absence of DNA damage; this does not lead to proteasomal degradation. In contrast, ubiquitination in response to DNA damage leads to proteasomal degradation By similarity.By similarity

    Keywords - PTMi

    Phosphoprotein, Ubl conjugation

    Interactioni

    Subunit structurei

    Monomer and dimer. Interacts with RAD51; regulates RAD51 recruitment and function at sites of DNA repair. Interacts with DSS1, WDR16, USP11, DMC1, ROCK2 and NPM1. Interacts with both nonubiquitinated and monoubiquitinated FANCD2; this complex also includes XRCC3 and phosphorylated FANCG. Part of a BRCA complex containing BRCA1, BRCA2 and PALB2. Interacts directly with PALB2 which may serve as a scaffold for a HR complex containing PALB2, BRCA2, RAD51C, RAD51 and XRCC3. Interacts with BRCA1 only in the presence of PALB2 which serves as the bridging protein. Interacts with POLH; the interaction is direct By similarity.By similarity

    Protein-protein interaction databases

    IntActiQ864S8. 1 interaction.
    MINTiMINT-8176579.

    Structurei

    3D structure databases

    ProteinModelPortaliQ864S8.
    SMRiQ864S8. Positions 1520-1552, 2423-3128.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati994 – 102835BRCA2 1Add
    BLAST
    Repeati1208 – 124235BRCA2 2Add
    BLAST
    Repeati1417 – 145135BRCA2 3Add
    BLAST
    Repeati1518 – 155235BRCA2 4Add
    BLAST
    Repeati1663 – 169735BRCA2 5Add
    BLAST
    Repeati1925 – 195935BRCA2 6Add
    BLAST
    Repeati1995 – 202935BRCA2 7Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 4040Interaction with PALB2By similarityAdd
    BLAST
    Regioni630 – 992363Interaction with NPM1By similarityAdd
    BLAST

    Sequence similaritiesi

    Contains 7 BRCA2 repeats.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    HOVERGENiHBG050731.
    KOiK08775.

    Family and domain databases

    Gene3Di2.40.50.140. 4 hits.
    InterProiIPR015525. BRCA2.
    IPR015252. BRCA2_hlx.
    IPR015187. BRCA2_OB_1.
    IPR015188. BRCA2_OB_3.
    IPR002093. BRCA2_repeat.
    IPR012340. NA-bd_OB-fold.
    IPR015205. Tower.
    [Graphical view]
    PANTHERiPTHR11289. PTHR11289. 1 hit.
    PfamiPF09169. BRCA-2_helical. 1 hit.
    PF09103. BRCA-2_OB1. 1 hit.
    PF09104. BRCA-2_OB3. 1 hit.
    PF00634. BRCA2. 7 hits.
    PF09121. Tower. 1 hit.
    [Graphical view]
    PIRSFiPIRSF002397. BRCA2. 1 hit.
    SUPFAMiSSF50249. SSF50249. 4 hits.
    SSF81872. SSF81872. 1 hit.
    PROSITEiPS50138. BRCA2_REPEAT. 7 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q864S8-1 [UniParc]FASTAAdd to Basket

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    MPIGCKERPT FFEIFRTRCN KADLGPISLN WFEELCLEAP PYNSEPTEES     50
    GYKISYEPNL FKTPQRKPCH QLASTPIIFK EQGLIPPIYQ QSPLKELGKD 100
    ITNSKHRSCC TMKSKMDQTN DVTSPPLNSC LSESPLLRST HVTPQREKSV 150
    VCGSLFHTPK LTKGQTPKRI SESLGAEVDP DMSWSSSLAT PPTLSSTVLI 200
    VRDEEASAAV FPNDTTAIFK SYFCNHDESL KKNDRFIPSG PDSENKSQRE 250
    AKSQGLGKMV GNSCDKVNSC KDPFGNSTLN VLEDGVRERV ADVSEEDSFP 300
    LCVPKCKTRN LQKIKTSKTR KNIFNETTDE CKEAKKQMKE NKHSFVSEME 350
    ANASDPLDSN VTNQKPFGNG SDKISKEVVL SSASESCHLT LSGLNGTHME 400
    KLPLLCISSC DQNNSEKDLI TTEKECTNFI ILEDSLPQIS GVPKCTEKIL 450
    NEEIVVNKID EGQCLESHED SILAVKQAVF ETSLIASPLQ GIRKSIFRIR 500
    ESPEETFSAV FSNNITDPNF KEEHEASESV LEKHSICSQK EDSLSTSSLD 550
    NGSWPATIKH TSVALKNSGL ISTLKKKTKK FIYVVNDETS YQGLKTQKDQ 600
    QSGLMNYSAQ FEANVLEGPL TFANADSGLL HSSVKKTCLQ NDSKEPILSL 650
    TNSFGTLLRK VSNKGSSSPN NKIISQDLDY KEAKIKKEKL QSFISTETNC 700
    LSSLQEKHCE DDTKSQRVAD RKEEILPAVS QPSVPYSEVE DSGIHFQTLK 750
    SFSSDPDKSS QLTPHPRDPP SNPVGLSRGR ESYEVSETLK CKNHEAGFEL 800
    TKTMENSQEI HVLNEHAKKA KLLSTEKYVT EASPSMKVPF NQNAHLTIIQ 850
    KDQKETTLIS KITMNPNSEE LFPDGDNFVF KITKERNVPV LGSIKELQDS 900
    DLCCVKEPVL ENSTMVVYTD MDDKQAAKVS ITKGFDSSNI DDLTEKDRNS 950
    IKQQLRMTLD QDSKSDITLD SDMKSNGNND YMDNWARLSD PILNHNFGNG 1000
    FRTASNKEIK LSEHNIKKSK MLFKDIEERY PTNLACIEIV NTPLESQEKL 1050
    SKPHILDPQS INTVSGCVQS SAYVSDSENR HTTPPTLSLK RDFDSNHNLT 1100
    PSQKAEITEL STILEESGSQ FEFTQFRKPS HLKQKNPCEM PEKHLTISNT 1150
    TPEEQKDGHL RLTINALSIS QGDSSKKFEG IIGGKQKLAC LSKTSCNKSA 1200
    SGHLTGKNEV EFRGFYSARG TKLNVCSEAL QKAKKLFSDL ENISEETSVE 1250
    VDRSFSSSKC NGSVSMFKKE NCNNEKKLNE KNNKYRLILQ NNIEMTTGIF 1300
    VAEDTEGYKR NIENKANKYT DASRNVYNFR EADGSDSSKN DTVYIHKEEN 1350
    GLPYIDQHDI DLKLSSQFIK EGNTQIKEGL SDLTCLEVVK AEETLHVNTS 1400
    NKEHLTANTM GRITKDFDIF DVSFQTASGK NIRVSRASLN KVTNLLDQKC 1450
    TEEELNNFAD SLNSELLSGI DINKADISHH GEMEILKKRQ MKESDLTGTE 1500
    NKSLTLQQRP EYEIKKIKEP TILGFHTASG KRIEIAKESL DKVKNLFDEQ 1550
    EQDKSEMTNF SHRGTKMSKG REECEGGLRL ACKTIEITPA SKEEEMQKPL 1600
    EKNLVSNEIV VVPRLLSDNL YKQTENLKIP NRASLKVKVH ENTGKETAKK 1650
    PTTCTNQSTY SATENSALSF YTGHGRKISV SQSSILEVKK WLRGGELDDQ 1700
    PEKTVYNISE YLPKSKVDNS GIEPVVRNVG ERENTSVSEI MFTVREADTD 1750
    PQSVNEDICV QRLVTNFSCK KENTAIKVTV SDSNNFDSTQ KLNSDSNDAV 1800
    PVYTTASSER VLVAHETKVA EGFTENCSMA IKQTTKSKPG KIVAGYRKAP 1850
    DDSEDTICPN SLDGAECSSP SHKDFAETQS EQTPQLNQSI SGFKKRSEIP 1900
    PHQINLKTSD ICKLSTGKRL QSISYTNACG IFSTASGKCV QVSDAALQKA 1950
    RQVFSKVEDS AKQPFSKVSF KHNEDHSDKF TREENTMIHT PQNLLSSAFS 2000
    GFSTASGKQV PVSESALCKV KGILEEFDVM RTECGPQRSP TSRQDVSKMP 2050
    PPSCVENKTP KHSVNSKLEK AYNKEFKLSS NSKIENGSSE NHSVQVSPYP 2100
    SQFKQDKQLI QGNKASLVEN IHLLEKEQAL PKNIKWKLET EAFPNLPLKT 2150
    DTAIHSTDSK DPENYFETET VEIAKAFMED GELTDADLLS HARHFLPTCQ 2200
    HSEETLVSNS RRGKRRGVLV SVGEPPIKRN LLNEFDRIIK NQEKSLKASK 2250
    STPDGIIKDR SLFMHHISLE PVTCGPFSTT KKRQEIQNPN FTAPGQKFLS 2300
    KSHFYEHLAL EKSSSNVSIS GQPFCTVPAT RSEKRGHSIT PSKPVKVFVP 2350
    PFKTKSRFLQ DEQHISKNTH VEENKQKPNN IDEHSSGDSK NNINNSEIHQ 2400
    LNKNNSSQAA TMVFTKCEKE PLDLIASLQN ARDIQDMRIR EKRKQHIFPQ 2450
    PGSLFLAKTS TVPRISLRVA VEGRVPSACS HKQLYMYGVS KHCVKINSKN 2500
    AESFQFHTQD YFGKEVQWAK EGIQLADGGW LIPSNDGKAG KEEFYRALCD 2550
    TPGVDPNLIS RIWVYNHYRW IIWKLAAMEF AFPKEFANRC LSPERVLLQL 2600
    KYRYDMEIDR SKRSAIKKIM ERDDTAAKTL VLCISETISS STDLSETSGS 2650
    KTSGVGTKNV GIVELTDGWY AIKAQLDPPL LALVKKGRLT VGHKIIIHGA 2700
    ELAGSPDACT PLEAPESLIL KISANSTRPA CWYAKLGFFP DPRPFPLPLS 2750
    SLFSDGGNVG CVDVVIQRTY PIQWMEKTPS GLCIFRNERE EEREATKYAE 2800
    AQQKKLEVLF NKIQAEFEKH DENITKRCVP LRALTRQQVC ALQDGAELYE 2850
    AVKNAPDPAS LEAYFSEEQI RALNNHRQML NDKKQAQIQL EFRKAMESAE 2900
    QGEQMLPRDV TTVWKMRIIS YGKKEKDSVT LSIWRPSSDL YSLLTEGKRY 2950
    RIYHLATSQS KSKSERAHIQ LTATKKTQYQ QLPASDELLF QVYQPREPLY 3000
    FNKLLDPDFQ PPCSEVDLIG FVVSVVKKIG FAPLVYLSDE CHNLLAIKVW 3050
    TDLNEDIVKP HTLIAASNLQ WRPESKSGIP TLFAGDFSRF SASPKEGHFQ 3100
    ETFHKMKNTI ENVETFCNDA ENKLVHILNA NSPKVSTPMK DYASEPHTIQ 3150
    TVLGLGNKLS MSSPNSEMNY QSPLSLCKPK AKSVPTPGSA QMTSKSCYKG 3200
    ERELDDPKTC KKRKALDFLS RLPLPPPVSP ICTFVSPAAQ KAFQPPRSCG 3250
    TKYETPIKKR ELNSPQMTPL KFNDTSLVES DSIADEELAL INTQALLSGL 3300
    AGEDQLMSLN DSPRTAPTSS KDYVRPKSYP TAPGIRDCEN PQASTEGGEP 3350
    DVQDTDTVKR SSMRLQRRQQ QT 3372
    Length:3,372
    Mass (Da):377,345
    Last modified:January 4, 2005 - v2
    Checksum:i37F23DA23CA94665
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB107955 mRNA. Translation: BAC75821.2.
    RefSeqiNP_001009858.1. NM_001009858.1.

    Genome annotation databases

    GeneIDi493878.
    KEGGifca:493878.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB107955 mRNA. Translation: BAC75821.2 .
    RefSeqi NP_001009858.1. NM_001009858.1.

    3D structure databases

    ProteinModelPortali Q864S8.
    SMRi Q864S8. Positions 1520-1552, 2423-3128.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q864S8. 1 interaction.
    MINTi MINT-8176579.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 493878.
    KEGGi fca:493878.

    Organism-specific databases

    CTDi 675.

    Phylogenomic databases

    HOVERGENi HBG050731.
    KOi K08775.

    Family and domain databases

    Gene3Di 2.40.50.140. 4 hits.
    InterProi IPR015525. BRCA2.
    IPR015252. BRCA2_hlx.
    IPR015187. BRCA2_OB_1.
    IPR015188. BRCA2_OB_3.
    IPR002093. BRCA2_repeat.
    IPR012340. NA-bd_OB-fold.
    IPR015205. Tower.
    [Graphical view ]
    PANTHERi PTHR11289. PTHR11289. 1 hit.
    Pfami PF09169. BRCA-2_helical. 1 hit.
    PF09103. BRCA-2_OB1. 1 hit.
    PF09104. BRCA-2_OB3. 1 hit.
    PF00634. BRCA2. 7 hits.
    PF09121. Tower. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF002397. BRCA2. 1 hit.
    SUPFAMi SSF50249. SSF50249. 4 hits.
    SSF81872. SSF81872. 1 hit.
    PROSITEi PS50138. BRCA2_REPEAT. 7 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Properties of the tumor suppressor gene brca2 in the cat."
      Oonuma T., Morimatsu M., Ochiai K., Syuto B.
      J. Vet. Med. Sci. 65:1123-1126(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].

    Entry informationi

    Entry nameiBRCA2_FELCA
    AccessioniPrimary (citable) accession number: Q864S8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 7, 2006
    Last sequence update: January 4, 2005
    Last modified: October 1, 2014
    This is version 65 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3