Q864S8 (BRCA2_FELCA) Reviewed, UniProtKB/Swiss-Prot
Last modified April 16, 2014. Version 63. History...
Names and origin
|Protein names||Recommended name:|
Breast cancer type 2 susceptibility protein homolog
Fanconi anemia group D1 protein homolog
|Organism||Felis catus (Cat) (Felis silvestris catus) [Reference proteome]|
|Taxonomic identifier||9685 [NCBI]|
|Taxonomic lineage||Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Carnivora › Feliformia › Felidae › Felinae › Felis|
|Sequence length||3372 AA.|
|Protein existence||Evidence at transcript level|
General annotation (Comments)
Involved in double-strand break repair and/or homologous recombination. Binds RAD51 and potentiates recombinational DNA repair by promoting assembly of RAD51 onto single-stranded DNA (ssDNA). Acts by targeting RAD51 to ssDNA over double-stranded DNA, enabling RAD51 to displace replication protein-A (RPA) from ssDNA and stabilizing RAD51-ssDNA filaments by blocking ATP hydrolysis. Part of a PALB2-scaffolded HR complex containing RAD51C and which is thought to play a role in DNA repair by HR. May participate in S phase checkpoint activation. Binds selectively to ssDNA, and to ssDNA in tailed duplexes and replication fork structures. May play a role in the extension step after strand invasion at replication-dependent DNA double-strand breaks; together with PALB2 is involved in both POLH localization at collapsed replication forks and DNA polymerization activity. In concert with NPM1, regulates centrosome duplication By similarity.
Monomer and dimer. Interacts with RAD51; regulates RAD51 recruitment and function at sites of DNA repair. Interacts with DSS1, WDR16, USP11, DMC1, ROCK2 and NPM1. Interacts with both nonubiquitinated and monoubiquitinated FANCD2; this complex also includes XRCC3 and phosphorylated FANCG. Part of a BRCA complex containing BRCA1, BRCA2 and PALB2. Interacts directly with PALB2 which may serve as a scaffold for a HR complex containing PALB2, BRCA2, RAD51C, RAD51 and XRCC3. Interacts with BRCA1 only in the presence of PALB2 which serves as the bridging protein. Interacts with POLH; the interaction is direct By similarity.
Phosphorylation by CHEK1 and CHEK2 regulates interaction with RAD51. Phosphorylation at Ser-3236 by CDK1 and CDK2 is low in S phase when recombination is active, but increases as cells progress towards mitosis; this phosphorylation prevents homologous recombination-dependent repair during S phase and G2 by inhibiting RAD51 binding By similarity.
Ubiquitinated in the absence of DNA damage; this does not lead to proteasomal degradation. In contrast, ubiquitination in response to DNA damage leads to proteasomal degradation By similarity.
Contains 7 BRCA2 repeats.
Sequence annotation (Features)
|Feature key||Position(s)||Length||Description||Graphical view||Feature identifier|
|Chain||1 – 3372||3372||Breast cancer type 2 susceptibility protein homolog||PRO_0000227014|
|Repeat||994 – 1028||35||BRCA2 1|
|Repeat||1208 – 1242||35||BRCA2 2|
|Repeat||1417 – 1451||35||BRCA2 3|
|Repeat||1518 – 1552||35||BRCA2 4|
|Repeat||1663 – 1697||35||BRCA2 5|
|Repeat||1925 – 1959||35||BRCA2 6|
|Repeat||1995 – 2029||35||BRCA2 7|
|Region||1 – 40||40||Interaction with PALB2 By similarity|
|Region||630 – 992||363||Interaction with NPM1 By similarity|
Amino acid modifications
|Modified residue||3236||1||Phosphoserine; by CDK1 and CDK2 By similarity|
|Modified residue||3331||1||Phosphothreonine; by CHEK1 and CHEK2 By similarity|
|AB107955 mRNA. Translation: BAC75821.2.|
|RefSeq||NP_001009858.1. NM_001009858.1. |
3D structure databases
|SMR||Q864S8. Positions 1520-1552, 2423-3128. |
Protein-protein interaction databases
|IntAct||Q864S8. 1 interaction.|
Protocols and materials databases
Genome annotation databases
Family and domain databases
|Gene3D||18.104.22.168. 4 hits. |
|InterPro||IPR015525. BRCA2. |
|PANTHER||PTHR11289. PTHR11289. 1 hit. |
|Pfam||PF09169. BRCA-2_helical. 1 hit. |
PF09103. BRCA-2_OB1. 1 hit.
PF09104. BRCA-2_OB3. 1 hit.
PF00634. BRCA2. 7 hits.
PF09121. Tower. 1 hit.
|PIRSF||PIRSF002397. BRCA2. 1 hit. |
|SUPFAM||SSF50249. SSF50249. 4 hits. |
SSF81872. SSF81872. 1 hit.
|PROSITE||PS50138. BRCA2_REPEAT. 7 hits. |
|Accession||Primary (citable) accession number: Q864S8|
|Entry status||Reviewed (UniProtKB/Swiss-Prot)|
|Annotation program||Chordata Protein Annotation Program|
Index of protein domains and families