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Q864S8

- BRCA2_FELCA

UniProt

Q864S8 - BRCA2_FELCA

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Protein

Breast cancer type 2 susceptibility protein homolog

Gene

BRCA2

Organism
Felis catus (Cat) (Felis silvestris catus)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Involved in double-strand break repair and/or homologous recombination. Binds RAD51 and potentiates recombinational DNA repair by promoting assembly of RAD51 onto single-stranded DNA (ssDNA). Acts by targeting RAD51 to ssDNA over double-stranded DNA, enabling RAD51 to displace replication protein-A (RPA) from ssDNA and stabilizing RAD51-ssDNA filaments by blocking ATP hydrolysis. Part of a PALB2-scaffolded HR complex containing RAD51C and which is thought to play a role in DNA repair by HR. May participate in S phase checkpoint activation. Binds selectively to ssDNA, and to ssDNA in tailed duplexes and replication fork structures. May play a role in the extension step after strand invasion at replication-dependent DNA double-strand breaks; together with PALB2 is involved in both POLH localization at collapsed replication forks and DNA polymerization activity. In concert with NPM1, regulates centrosome duplication. Interacts with the TREX-2 complex (transcription and export complex 2) subunits PCID2 and DSS1, and is required to prevent R-loop-associated DNA damage and thus transcription-associated genomic instability, independently of its known role in homologous recombination (By similarity).By similarity

GO - Molecular functioni

  1. gamma-tubulin binding Source: UniProtKB
  2. single-stranded DNA binding Source: InterPro

GO - Biological processi

  1. centrosome duplication Source: UniProtKB
  2. cytokinesis Source: UniProtKB
  3. double-strand break repair Source: UniProtKB
  4. double-strand break repair via homologous recombination Source: InterPro
  5. negative regulation of mammary gland epithelial cell proliferation Source: UniProtKB
  6. nucleotide-excision repair Source: UniProtKB
  7. positive regulation of transcription, DNA-templated Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Breast cancer type 2 susceptibility protein homolog
Alternative name(s):
Fanconi anemia group D1 protein homolog
Gene namesi
Name:BRCA2
Synonyms:FANCD1
OrganismiFelis catus (Cat) (Felis silvestris catus)
Taxonomic identifieri9685 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraFeliformiaFelidaeFelinaeFelis
ProteomesiUP000011712: Unplaced

Subcellular locationi

Nucleus By similarity. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity

GO - Cellular componenti

  1. BRCA2-MAGE-D1 complex Source: UniProtKB
  2. centrosome Source: UniProtKB
  3. nucleus Source: UniProtKB
  4. protein complex Source: UniProtKB
  5. secretory granule Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

Pathology & Biotechi

Keywords - Diseasei

Tumor suppressor

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 33723372Breast cancer type 2 susceptibility protein homologPRO_0000227014Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei3236 – 32361Phosphoserine; by CDK1 and CDK2By similarity
Modified residuei3331 – 33311Phosphothreonine; by CHEK1 and CHEK2By similarity

Post-translational modificationi

Phosphorylation by CHEK1 and CHEK2 regulates interaction with RAD51. Phosphorylation at Ser-3236 by CDK1 and CDK2 is low in S phase when recombination is active, but increases as cells progress towards mitosis; this phosphorylation prevents homologous recombination-dependent repair during S phase and G2 by inhibiting RAD51 binding (By similarity).By similarity
Ubiquitinated in the absence of DNA damage; this does not lead to proteasomal degradation. In contrast, ubiquitination in response to DNA damage leads to proteasomal degradation (By similarity).By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Interactioni

Subunit structurei

Monomer and dimer. Interacts with RAD51; regulates RAD51 recruitment and function at sites of DNA repair. Interacts with DSS1, WDR16, USP11, DMC1, ROCK2 and NPM1. Interacts with both nonubiquitinated and monoubiquitinated FANCD2; this complex also includes XRCC3 and phosphorylated FANCG. Part of a BRCA complex containing BRCA1, BRCA2 and PALB2. Interacts directly with PALB2 which may serve as a scaffold for a HR complex containing PALB2, BRCA2, RAD51C, RAD51 and XRCC3. Interacts with BRCA1 only in the presence of PALB2 which serves as the bridging protein. Interacts with POLH; the interaction is direct (By similarity). Interacts with the TREX-2 complex subunits PCID2 and DSS1 (By similarity).By similarity

Protein-protein interaction databases

IntActiQ864S8. 1 interaction.
MINTiMINT-8176579.

Structurei

3D structure databases

ProteinModelPortaliQ864S8.
SMRiQ864S8. Positions 1520-1552, 2423-3128.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati994 – 102835BRCA2 1Add
BLAST
Repeati1208 – 124235BRCA2 2Add
BLAST
Repeati1417 – 145135BRCA2 3Add
BLAST
Repeati1518 – 155235BRCA2 4Add
BLAST
Repeati1663 – 169735BRCA2 5Add
BLAST
Repeati1925 – 195935BRCA2 6Add
BLAST
Repeati1995 – 202935BRCA2 7Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 4040Interaction with PALB2By similarityAdd
BLAST
Regioni630 – 992363Interaction with NPM1By similarityAdd
BLAST

Sequence similaritiesi

Contains 7 BRCA2 repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

HOVERGENiHBG050731.
InParanoidiQ864S8.
KOiK08775.

Family and domain databases

Gene3Di2.40.50.140. 4 hits.
InterProiIPR015525. BRCA2.
IPR015252. BRCA2_hlx.
IPR015187. BRCA2_OB_1.
IPR015188. BRCA2_OB_3.
IPR002093. BRCA2_repeat.
IPR012340. NA-bd_OB-fold.
IPR015205. Tower.
[Graphical view]
PANTHERiPTHR11289. PTHR11289. 1 hit.
PfamiPF09169. BRCA-2_helical. 1 hit.
PF09103. BRCA-2_OB1. 1 hit.
PF09104. BRCA-2_OB3. 1 hit.
PF00634. BRCA2. 7 hits.
PF09121. Tower. 1 hit.
[Graphical view]
PIRSFiPIRSF002397. BRCA2. 1 hit.
SUPFAMiSSF50249. SSF50249. 4 hits.
SSF81872. SSF81872. 1 hit.
PROSITEiPS50138. BRCA2_REPEAT. 7 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q864S8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPIGCKERPT FFEIFRTRCN KADLGPISLN WFEELCLEAP PYNSEPTEES
60 70 80 90 100
GYKISYEPNL FKTPQRKPCH QLASTPIIFK EQGLIPPIYQ QSPLKELGKD
110 120 130 140 150
ITNSKHRSCC TMKSKMDQTN DVTSPPLNSC LSESPLLRST HVTPQREKSV
160 170 180 190 200
VCGSLFHTPK LTKGQTPKRI SESLGAEVDP DMSWSSSLAT PPTLSSTVLI
210 220 230 240 250
VRDEEASAAV FPNDTTAIFK SYFCNHDESL KKNDRFIPSG PDSENKSQRE
260 270 280 290 300
AKSQGLGKMV GNSCDKVNSC KDPFGNSTLN VLEDGVRERV ADVSEEDSFP
310 320 330 340 350
LCVPKCKTRN LQKIKTSKTR KNIFNETTDE CKEAKKQMKE NKHSFVSEME
360 370 380 390 400
ANASDPLDSN VTNQKPFGNG SDKISKEVVL SSASESCHLT LSGLNGTHME
410 420 430 440 450
KLPLLCISSC DQNNSEKDLI TTEKECTNFI ILEDSLPQIS GVPKCTEKIL
460 470 480 490 500
NEEIVVNKID EGQCLESHED SILAVKQAVF ETSLIASPLQ GIRKSIFRIR
510 520 530 540 550
ESPEETFSAV FSNNITDPNF KEEHEASESV LEKHSICSQK EDSLSTSSLD
560 570 580 590 600
NGSWPATIKH TSVALKNSGL ISTLKKKTKK FIYVVNDETS YQGLKTQKDQ
610 620 630 640 650
QSGLMNYSAQ FEANVLEGPL TFANADSGLL HSSVKKTCLQ NDSKEPILSL
660 670 680 690 700
TNSFGTLLRK VSNKGSSSPN NKIISQDLDY KEAKIKKEKL QSFISTETNC
710 720 730 740 750
LSSLQEKHCE DDTKSQRVAD RKEEILPAVS QPSVPYSEVE DSGIHFQTLK
760 770 780 790 800
SFSSDPDKSS QLTPHPRDPP SNPVGLSRGR ESYEVSETLK CKNHEAGFEL
810 820 830 840 850
TKTMENSQEI HVLNEHAKKA KLLSTEKYVT EASPSMKVPF NQNAHLTIIQ
860 870 880 890 900
KDQKETTLIS KITMNPNSEE LFPDGDNFVF KITKERNVPV LGSIKELQDS
910 920 930 940 950
DLCCVKEPVL ENSTMVVYTD MDDKQAAKVS ITKGFDSSNI DDLTEKDRNS
960 970 980 990 1000
IKQQLRMTLD QDSKSDITLD SDMKSNGNND YMDNWARLSD PILNHNFGNG
1010 1020 1030 1040 1050
FRTASNKEIK LSEHNIKKSK MLFKDIEERY PTNLACIEIV NTPLESQEKL
1060 1070 1080 1090 1100
SKPHILDPQS INTVSGCVQS SAYVSDSENR HTTPPTLSLK RDFDSNHNLT
1110 1120 1130 1140 1150
PSQKAEITEL STILEESGSQ FEFTQFRKPS HLKQKNPCEM PEKHLTISNT
1160 1170 1180 1190 1200
TPEEQKDGHL RLTINALSIS QGDSSKKFEG IIGGKQKLAC LSKTSCNKSA
1210 1220 1230 1240 1250
SGHLTGKNEV EFRGFYSARG TKLNVCSEAL QKAKKLFSDL ENISEETSVE
1260 1270 1280 1290 1300
VDRSFSSSKC NGSVSMFKKE NCNNEKKLNE KNNKYRLILQ NNIEMTTGIF
1310 1320 1330 1340 1350
VAEDTEGYKR NIENKANKYT DASRNVYNFR EADGSDSSKN DTVYIHKEEN
1360 1370 1380 1390 1400
GLPYIDQHDI DLKLSSQFIK EGNTQIKEGL SDLTCLEVVK AEETLHVNTS
1410 1420 1430 1440 1450
NKEHLTANTM GRITKDFDIF DVSFQTASGK NIRVSRASLN KVTNLLDQKC
1460 1470 1480 1490 1500
TEEELNNFAD SLNSELLSGI DINKADISHH GEMEILKKRQ MKESDLTGTE
1510 1520 1530 1540 1550
NKSLTLQQRP EYEIKKIKEP TILGFHTASG KRIEIAKESL DKVKNLFDEQ
1560 1570 1580 1590 1600
EQDKSEMTNF SHRGTKMSKG REECEGGLRL ACKTIEITPA SKEEEMQKPL
1610 1620 1630 1640 1650
EKNLVSNEIV VVPRLLSDNL YKQTENLKIP NRASLKVKVH ENTGKETAKK
1660 1670 1680 1690 1700
PTTCTNQSTY SATENSALSF YTGHGRKISV SQSSILEVKK WLRGGELDDQ
1710 1720 1730 1740 1750
PEKTVYNISE YLPKSKVDNS GIEPVVRNVG ERENTSVSEI MFTVREADTD
1760 1770 1780 1790 1800
PQSVNEDICV QRLVTNFSCK KENTAIKVTV SDSNNFDSTQ KLNSDSNDAV
1810 1820 1830 1840 1850
PVYTTASSER VLVAHETKVA EGFTENCSMA IKQTTKSKPG KIVAGYRKAP
1860 1870 1880 1890 1900
DDSEDTICPN SLDGAECSSP SHKDFAETQS EQTPQLNQSI SGFKKRSEIP
1910 1920 1930 1940 1950
PHQINLKTSD ICKLSTGKRL QSISYTNACG IFSTASGKCV QVSDAALQKA
1960 1970 1980 1990 2000
RQVFSKVEDS AKQPFSKVSF KHNEDHSDKF TREENTMIHT PQNLLSSAFS
2010 2020 2030 2040 2050
GFSTASGKQV PVSESALCKV KGILEEFDVM RTECGPQRSP TSRQDVSKMP
2060 2070 2080 2090 2100
PPSCVENKTP KHSVNSKLEK AYNKEFKLSS NSKIENGSSE NHSVQVSPYP
2110 2120 2130 2140 2150
SQFKQDKQLI QGNKASLVEN IHLLEKEQAL PKNIKWKLET EAFPNLPLKT
2160 2170 2180 2190 2200
DTAIHSTDSK DPENYFETET VEIAKAFMED GELTDADLLS HARHFLPTCQ
2210 2220 2230 2240 2250
HSEETLVSNS RRGKRRGVLV SVGEPPIKRN LLNEFDRIIK NQEKSLKASK
2260 2270 2280 2290 2300
STPDGIIKDR SLFMHHISLE PVTCGPFSTT KKRQEIQNPN FTAPGQKFLS
2310 2320 2330 2340 2350
KSHFYEHLAL EKSSSNVSIS GQPFCTVPAT RSEKRGHSIT PSKPVKVFVP
2360 2370 2380 2390 2400
PFKTKSRFLQ DEQHISKNTH VEENKQKPNN IDEHSSGDSK NNINNSEIHQ
2410 2420 2430 2440 2450
LNKNNSSQAA TMVFTKCEKE PLDLIASLQN ARDIQDMRIR EKRKQHIFPQ
2460 2470 2480 2490 2500
PGSLFLAKTS TVPRISLRVA VEGRVPSACS HKQLYMYGVS KHCVKINSKN
2510 2520 2530 2540 2550
AESFQFHTQD YFGKEVQWAK EGIQLADGGW LIPSNDGKAG KEEFYRALCD
2560 2570 2580 2590 2600
TPGVDPNLIS RIWVYNHYRW IIWKLAAMEF AFPKEFANRC LSPERVLLQL
2610 2620 2630 2640 2650
KYRYDMEIDR SKRSAIKKIM ERDDTAAKTL VLCISETISS STDLSETSGS
2660 2670 2680 2690 2700
KTSGVGTKNV GIVELTDGWY AIKAQLDPPL LALVKKGRLT VGHKIIIHGA
2710 2720 2730 2740 2750
ELAGSPDACT PLEAPESLIL KISANSTRPA CWYAKLGFFP DPRPFPLPLS
2760 2770 2780 2790 2800
SLFSDGGNVG CVDVVIQRTY PIQWMEKTPS GLCIFRNERE EEREATKYAE
2810 2820 2830 2840 2850
AQQKKLEVLF NKIQAEFEKH DENITKRCVP LRALTRQQVC ALQDGAELYE
2860 2870 2880 2890 2900
AVKNAPDPAS LEAYFSEEQI RALNNHRQML NDKKQAQIQL EFRKAMESAE
2910 2920 2930 2940 2950
QGEQMLPRDV TTVWKMRIIS YGKKEKDSVT LSIWRPSSDL YSLLTEGKRY
2960 2970 2980 2990 3000
RIYHLATSQS KSKSERAHIQ LTATKKTQYQ QLPASDELLF QVYQPREPLY
3010 3020 3030 3040 3050
FNKLLDPDFQ PPCSEVDLIG FVVSVVKKIG FAPLVYLSDE CHNLLAIKVW
3060 3070 3080 3090 3100
TDLNEDIVKP HTLIAASNLQ WRPESKSGIP TLFAGDFSRF SASPKEGHFQ
3110 3120 3130 3140 3150
ETFHKMKNTI ENVETFCNDA ENKLVHILNA NSPKVSTPMK DYASEPHTIQ
3160 3170 3180 3190 3200
TVLGLGNKLS MSSPNSEMNY QSPLSLCKPK AKSVPTPGSA QMTSKSCYKG
3210 3220 3230 3240 3250
ERELDDPKTC KKRKALDFLS RLPLPPPVSP ICTFVSPAAQ KAFQPPRSCG
3260 3270 3280 3290 3300
TKYETPIKKR ELNSPQMTPL KFNDTSLVES DSIADEELAL INTQALLSGL
3310 3320 3330 3340 3350
AGEDQLMSLN DSPRTAPTSS KDYVRPKSYP TAPGIRDCEN PQASTEGGEP
3360 3370
DVQDTDTVKR SSMRLQRRQQ QT
Length:3,372
Mass (Da):377,345
Last modified:January 4, 2005 - v2
Checksum:i37F23DA23CA94665
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB107955 mRNA. Translation: BAC75821.2.
RefSeqiNP_001009858.1. NM_001009858.1.

Genome annotation databases

GeneIDi493878.
KEGGifca:493878.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB107955 mRNA. Translation: BAC75821.2 .
RefSeqi NP_001009858.1. NM_001009858.1.

3D structure databases

ProteinModelPortali Q864S8.
SMRi Q864S8. Positions 1520-1552, 2423-3128.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q864S8. 1 interaction.
MINTi MINT-8176579.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 493878.
KEGGi fca:493878.

Organism-specific databases

CTDi 675.

Phylogenomic databases

HOVERGENi HBG050731.
InParanoidi Q864S8.
KOi K08775.

Family and domain databases

Gene3Di 2.40.50.140. 4 hits.
InterProi IPR015525. BRCA2.
IPR015252. BRCA2_hlx.
IPR015187. BRCA2_OB_1.
IPR015188. BRCA2_OB_3.
IPR002093. BRCA2_repeat.
IPR012340. NA-bd_OB-fold.
IPR015205. Tower.
[Graphical view ]
PANTHERi PTHR11289. PTHR11289. 1 hit.
Pfami PF09169. BRCA-2_helical. 1 hit.
PF09103. BRCA-2_OB1. 1 hit.
PF09104. BRCA-2_OB3. 1 hit.
PF00634. BRCA2. 7 hits.
PF09121. Tower. 1 hit.
[Graphical view ]
PIRSFi PIRSF002397. BRCA2. 1 hit.
SUPFAMi SSF50249. SSF50249. 4 hits.
SSF81872. SSF81872. 1 hit.
PROSITEi PS50138. BRCA2_REPEAT. 7 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Properties of the tumor suppressor gene brca2 in the cat."
    Oonuma T., Morimatsu M., Ochiai K., Syuto B.
    J. Vet. Med. Sci. 65:1123-1126(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiBRCA2_FELCA
AccessioniPrimary (citable) accession number: Q864S8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: January 4, 2005
Last modified: October 29, 2014
This is version 66 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3