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Q86218 (VP2_ROTSR) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 38. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein attributes

Sequence length882 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Inner capsid protein that self assembles to form an icosahedral capsid with a T=2 symmetry, which consists of 120 copies of VP2, with channels at each of its five-fold vertices. This capsid constitutes the innermost concentric layer of the viral mature particle. It encapsidates the polymerase VP1, the capping enzyme VP3 and the genomic dsRNA, thereby defining the core. The innermost VP2 capsid and the intermediate VP6 capsid remain intact following cell entry to protect the dsRNA from degradation and to prevent unfavorable antiviral responses in the host cell during all the replication cycle of the virus. Nacent transcripts are transcribed within the structural confines of this double-layered particle (DLP) and are extruded through the channels at the five-fold axes. VP2 is required for the replicase activity of VP1 polymerase. It probably plays a role in the coordination of packaging and genome replication by controlling the initiation of minus-strand synthesis. Binding to the polymerase VP1 presumably activates the autoinhibited VP1-RNA complex which will start the synthesis of the complementary minus-strand By similarity.

Subunit structure

Dimer Potential. Interacts with VP1; this interaction presumably activates VP1. Interacts with VP3. Interacts with VP6. Interacts with NSP5 By similarity.

Subcellular location

Virion Potential. Note: Inner capsid protein. Also found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging.

Domain

The N-terminus is involved in VP1 binding By similarity.

Sequence similarities

Belongs to the rotavirus VP2 family.

Ontologies

Keywords
   Cellular componentCapsid protein
Inner capsid protein
Virion
   DomainRepeat
   LigandRNA-binding
Gene Ontology (GO)
   Cellular_componentviral inner capsid

Inferred from electronic annotation. Source: UniProtKB-KW

viral nucleocapsid

Inferred from electronic annotation. Source: InterPro

   Molecular_functionRNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 882882Inner capsid protein VP2
PRO_0000368065

Regions

Region1 – 9494Interaction with VP1 and VP3 By similarity
Region396 – 41621Hydrophobic By similarity
Region424 – 44421Hydrophobic By similarity
Region538 – 55922Leucine-zipper 1 Potential
Region667 – 68822Leucine-zipper 2 Potential

Sequences

Sequence LengthMass (Da)Tools
Q86218 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 8221C2211B35681D

FASTA882102,669
        10         20         30         40         50         60 
MAYRKRGARR ETNLKQDERM QEKEDSKNIN NDSPKSQLSE KVLSKKEEII TDNQEEVKIS 

        70         80         90        100        110        120 
DEVKKSNKEE SKQLLEVLKT KEEHQKEVQY EILQKTIPTF EPKESILKKL EDIKPEQAKK 

       130        140        150        160        170        180 
QTKLFRIFEP KQLPIYRANG ERELRNRWYW KLKRDTLPDG DYDVREYFLN LYDQVLMEMP 

       190        200        210        220        230        240 
DYLLLKDMAV ENKNSRDAGK VVDSETAAIC DAIFQDEETE GAVRRFIAEM RQRVQADRNV 

       250        260        270        280        290        300 
VNYPSILHPI DHAFNGYFLQ HQLVEPLNND IIFNYIPERI RNDVNYILNM DRNLPSTARY 

       310        320        330        340        350        360 
IRPNLLQDRL NLHDNFESLW DTITTSNYIL ARSVVPDLKE LVSTEAQIQK MSQDLQLEAL 

       370        380        390        400        410        420 
TIQSETQFLT GINSQAANDC FKTLIAAMLS QRTMSLDFVT TNYMSLISGM WLLTVIPNDM 

       430        440        450        460        470        480 
FIRESLVACQ LAIINTIVYP AFGMQRMHYR NGDPQTPFQI AEQQIQNFQV ANWLHFVNYN 

       490        500        510        520        530        540 
QFRQVVIDGV LNQVLNDNIR NGHVVNQLME ALMQLSRQQF PTMPVDYKRS IQRGILLLSN 

       550        560        570        580        590        600 
RLGQLVDLTR LLSYNYETLM ACITMNMQHV QTLTTEKLQL TSVTSLCMLI GNATVIPSPQ 

       610        620        630        640        650        660 
TLFHYYNVNV NFHSNYNERI NDAVAIITAA NRLNLYQKKM KSIVEDFLKR LQIFDVARVP 

       670        680        690        700        710        720 
DDQMYRLRDR LRLLPVEIRR LDIFNLIAMN MEQIERASDK IAQGVIIAYR DMQLERDEMY 

       730        740        750        760        770        780 
GYVNIARNLD GFQQINLEEL MRSGDYAQIT NMLLNNQPVA LVGALPFITD SSVISLIAKL 

       790        800        810        820        830        840 
DATVFAQIVK LRKVDTLKPI LYKINSDSND FYLVANYDWI PTSTTKVYKQ VPQQFDFRAS 

       850        860        870        880 
MHMLTSNLTF TVYSDLLAFV SADTVEPINA VAFDNMRIMN EL 

« Hide

References

[1]"Temperature-sensitive lesions in the capsid proteins of the rotavirus mutants tsF and tsG that affect virion assembly."
Mansell E.A., Ramig R.F., Patton J.T.
Virology 204:69-81(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L33364 Genomic RNA. Translation: AAA47349.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR007779. Rotavirus_VP2.
[Graphical view]
PfamPF05087. Rota_VP2. 1 hit.
[Graphical view]
ProDomPD008866. Rota_VP2. 1 hit.
[Graphical view] [Entries sharing at least one domain]
ProtoNetSearch...

Entry information

Entry nameVP2_ROTSR
AccessionPrimary (citable) accession number: Q86218
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: November 1, 1996
Last modified: October 16, 2013
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families