Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Tumor necrosis factor ligand superfamily member 6

Gene

FASLG

Organism
Felis catus (Cat) (Felis silvestris catus)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells. Involved in cytotoxic T-cell-mediated apoptosis, natural killer cell-mediated apoptosis and in T-cell development. Initiates fratricidal/suicidal activation-induced cell death (AICD) in antigen-activated T-cells contributing to the termination of immune responses. TNFRSF6/FAS-mediated apoptosis has also a role in the induction of peripheral tolerance. Binds to TNFRSF6B/DcR3, a decoy receptor that blocks apoptosis.By similarity
Tumor necrosis factor ligand superfamily member 6, soluble form: Induces FAS-mediated activation of NF-kappa-B, initiating non-apoptotic signaling pathways. Can induce apoptosis but does not appear to be essential for this process.By similarity
FasL intracellular domain: Cytoplasmic form induces gene transcription inhibition.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionCytokine, Repressor
Biological processApoptosis, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Tumor necrosis factor ligand superfamily member 6
Alternative name(s):
Fas antigen ligand
Short name:
Fas ligand
Short name:
FasL
CD_antigen: CD178
Cleaved into the following 4 chains:
Alternative name(s):
Receptor-binding FasL ectodomain
Soluble Fas ligand
Short name:
sFasL
FasL intracellular domain
Short name:
FasL ICD
Alternative name(s):
SPPL2A-processed FasL form
Short name:
SPA
Gene namesi
Name:FASLG
Synonyms:FASL, TNFSF6
OrganismiFelis catus (Cat) (Felis silvestris catus)
Taxonomic identifieri9685 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraFeliformiaFelidaeFelinaeFelis
Proteomesi
  • UP000011712 Componenti: Chromosome F1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 80CytoplasmicSequence analysisAdd BLAST80
Transmembranei81 – 101Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini102 – 280ExtracellularSequence analysisAdd BLAST179

Keywords - Cellular componenti

Cell membrane, Cytoplasmic vesicle, Lysosome, Membrane, Nucleus, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002270111 – 280Tumor necrosis factor ligand superfamily member 6, membrane formAdd BLAST280
ChainiPRO_00004171501 – 128ADAM10-processed FasL formBy similarityAdd BLAST128
ChainiPRO_00004171511 – 81FasL intracellular domainBy similarityAdd BLAST81
ChainiPRO_0000227012129 – 280Tumor necrosis factor ligand superfamily member 6, soluble formBy similarityAdd BLAST152

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi183N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi201 ↔ 232By similarity
Glycosylationi249N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi259N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

The soluble form derives from the membrane form by proteolytic processing. The membrane-bound form undergoes two successive intramembrane proteolytic cleavages. The first one is processed by ADAM10 producing an N-terminal fragment, which lacks the receptor-binding extracellular domain. This ADAM10-processed FasL (FasL APL) remnant form is still membrane anchored and further processed by SPPL2A that liberates the FasL intracellular domain (FasL ICD). FasL shedding by ADAM10 is a prerequisite for subsequent intramembrane cleavage by SPPL2A in T-cells.By similarity
Phosphorylated by FGR on tyrosine residues; this is required for ubiquitination and subsequent internalization.By similarity
N-glycosylated. Glycosylation enhances apoptotic activity.By similarity
Monoubiquitinated.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei80 – 81Cleavage; by SPPL2ABy similarity2
Sitei128 – 129Cleavage; by ADAM10By similarity2

Keywords - PTMi

Disulfide bond, Glycoprotein, Ubl conjugation

Expressioni

Gene expression databases

BgeeiENSFCAG00000005849.

Interactioni

Subunit structurei

Homotrimer. Interacts with ARHGAP9, BAIAP2L1, BTK, CACNB3, CACNB4, CRK, DLG2, DNMBP, DOCK4, EPS8L3, FGR, FYB1, FYN, HCK, ITK, ITSN2, KALRN, LYN, MACC1, MIA, MPP4, MYO15A, NCF1, NCK1, NCK2, NCKIPSD, OSTF1, PIK3R1, PSTPIP1, RIMBP3C, SAMSN1, SH3GL3, SH3PXD2B, SH3PXD2A, SH3RF2, SKAP2, SNX33, SNX9, SORBS3, SPTA1, SRC, SRGAP1, SRGAP2, SRGAP3, TEC, TJP3 and YES1.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9685.ENSFCAP00000005431.

Structurei

3D structure databases

ProteinModelPortaliQ861W5.
SMRiQ861W5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi4 – 69Pro-richAdd BLAST66
Compositional biasi45 – 64Poly-ProAdd BLAST20

Sequence similaritiesi

Belongs to the tumor necrosis factor family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IHZF. Eukaryota.
ENOG4112D9R. LUCA.
GeneTreeiENSGT00530000062992.
HOVERGENiHBG055128.
InParanoidiQ861W5.
KOiK04389.
OMAiWEDTYGI.
OrthoDBiEOG091G0MFC.

Family and domain databases

Gene3Di2.60.120.40. 1 hit.
InterProiView protein in InterPro
IPR028326. FASL.
IPR006053. TNF.
IPR021184. TNF_CS.
IPR006052. TNF_dom.
IPR008983. Tumour_necrosis_fac-like_dom.
PfamiView protein in Pfam
PF00229. TNF. 1 hit.
PRINTSiPR01681. FASLIGAND.
PR01234. TNECROSISFCT.
SMARTiView protein in SMART
SM00207. TNF. 1 hit.
SUPFAMiSSF49842. SSF49842. 1 hit.
PROSITEiView protein in PROSITE
PS00251. TNF_1. 1 hit.
PS50049. TNF_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q861W5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQQPLNYPYP QIYWVDSRAS SPWGPPGSVL PCPSSVPGRP GQRRPPPPPP
60 70 80 90 100
PTLPPPPPPP PLPPLPLPPL KTRRDHNTGL CLLVMFFMVL VALVGLGLGM
110 120 130 140 150
FQLFHLQKEL AELRESTSQK HVASSLEKQI GQLNPPSEKR ELRKVAHLTG
160 170 180 190 200
KPNSRSIPLE WEDTYGIALV SGVKYKKGGL VINDTGMYFV YSKVNFRGQS
210 220 230 240 250
CNNQPLNHKV YMRNSKYPQD LVLMEGKMMN YCTTGQMWAR SSYLGAVFNL
260 270 280
TSADHLYVNV SELSLVSFEE SKTFFGLYKL
Length:280
Mass (Da):31,361
Last modified:June 1, 2003 - v1
Checksum:i6AA7E2DE1F1A6B5C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB009280 mRNA. Translation: BAC76426.1.
RefSeqiNP_001009352.1. NM_001009352.1.

Genome annotation databases

EnsembliENSFCAT00000005851; ENSFCAP00000005431; ENSFCAG00000005849.
GeneIDi493945.
KEGGifca:493945.

Similar proteinsi

Entry informationi

Entry nameiTNFL6_FELCA
AccessioniPrimary (citable) accession number: Q861W5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: June 1, 2003
Last modified: September 27, 2017
This is version 87 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families