Q86197 (NSP2_ROTGA) Reviewed, UniProtKB/Swiss-Prot
Last modified July 13, 2010. Version 36. History...
Names and origin
|Protein names||Recommended name:|
Non-structural protein 2
|Organism||Rotavirus B (isolate Human/China/ADRV/1982) (RV-B) (Rotavirus B (isolate adult diarrhea rotavirus))|
|Taxonomic identifier||10942 [NCBI]|
|Taxonomic lineage||Viruses › dsRNA viruses › Reoviridae › Sedoreovirinae › Rotavirus ›|
|Virus host||Homo sapiens (Human) [TaxID: 9606]|
|Sequence length||279 AA.|
|Protein existence||Evidence at transcript level|
General annotation (Comments)
Involved in genome replication and packaging. Plays a crucial role, together with NSP5, in the formation of virus factories (viroplasms) which are large inclusions in the cytoplasm where replication intermediates are assembled and RNA replication takes place. Displays ssRNA binding, NTPase, RNA triphosphatase (RTPase) and ATP-independent helix-unwinding activity activities. The unwiding activity may prepare and organize plus-strand RNAs for packaging and replication by removing interfering secondary structures. Unlike typical helicases, NSP2 requires neither a divalent cation nor a nucleotide energy source for helix destabilization. The RTPase activity may account for the absence of the 5'-terminal gamma-phosphate on the minus-strands of dsRNA genome segments By similarity.
Homooctamer By similarity. Interacts with NSP5; this interaction leads to formation of virus factories By similarity.
Host cytoplasm Potential. Note: Found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging By similarity.
Belongs to the rotavirus NSP2 family.
|Cellular component||Host cytoplasm|
|Gene Ontology (GO)|
|Cellular_component||host cell cytoplasm|
Inferred from electronic annotation. Source: UniProtKB-SubCell
Inferred from electronic annotation. Source: UniProtKB-KWhydrolase activity
Inferred from electronic annotation. Source: UniProtKB-KWnucleotide binding
Inferred from electronic annotation. Source: UniProtKB-KW
|Complete GO annotation...|
Sequence annotation (Features)
|Feature key||Position(s)||Length||Description||Graphical view||Feature identifier|
|Chain||1 – 279||279||Non-structural protein 2||PRO_0000369851|
|Region||214 – 249||36||RNA-binding Potential|
|Active site||233||1||For NTPase activity By similarity|
|Sequence conflict||60||1||C → S in CAI30287. Ref.2|
|Sequence conflict||63||1||P → L in CAI30287. Ref.2|
|Sequence conflict||84||1||M → I in CAI30287. Ref.2|
|Sequence conflict||105||1||N → S in CAI30287. Ref.2|
|Sequence conflict||142||1||R → H in CAI30287. Ref.2|
|Sequence conflict||146||1||G → S in CAI30287. Ref.2|
|Sequence conflict||175||1||S → T in CAI30287. Ref.2|
|||"The evolution of human group B rotaviruses: correction and an update."|
Jiang B., Wang Y., Glass R.I., Fang Z.-Y.
J. Clin. Virol. 34:158-159(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
|||"Primary identification of the eighth RNA segment of the group B rotavirus ADRV."|
Mackow E.R., Chen G., Werner R., Fay M.E., Tao H.
Submitted (SEP-1992) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
|Accession||Primary (citable) accession number: Q86197|
Secondary accession number(s): Q5K038
|Entry status||Reviewed (UniProtKB/Swiss-Prot)|
|Annotation program||Viral Protein Annotation Program|
Index of protein domains and families