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Protein

Non-structural protein 2

Gene
N/A
Organism
Rotavirus B (isolate Human/China/ADRV/1982) (RV-B) (Rotavirus B (isolate adult diarrhea rotavirus))
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in genome replication and packaging. Plays a crucial role, together with NSP5, in the formation of virus factories (viroplasms) which are large inclusions in the cytoplasm where replication intermediates are assembled and RNA replication takes place. Displays ssRNA binding, NTPase, RNA triphosphatase (RTPase) and ATP-independent helix-unwinding activity activities. The unwiding activity may prepare and organize plus-strand RNAs for packaging and replication by removing interfering secondary structures. Unlike typical helicases, NSP2 requires neither a divalent cation nor a nucleotide energy source for helix destabilization. The RTPase activity may account for the absence of the 5'-terminal gamma-phosphate on the minus-strands of dsRNA genome segments (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei233 – 2331For NTPase activityBy similarity

GO - Molecular functioni

  1. hydrolase activity Source: UniProtKB-KW
  2. nucleotide binding Source: UniProtKB-KW
  3. RNA binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Non-structural protein 2 (EC:3.6.4.-)
Short name:
NSP2
OrganismiRotavirus B (isolate Human/China/ADRV/1982) (RV-B) (Rotavirus B (isolate adult diarrhea rotavirus))
Taxonomic identifieri10942 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]

Subcellular locationi

  1. Host cytoplasm Curated

  2. Note: Found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging.By similarity

GO - Cellular componenti

  1. host cell cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 279279Non-structural protein 2PRO_0000369851Add
BLAST

Interactioni

Subunit structurei

Homooctamer. Interacts with NSP5; this interaction leads to formation of virus factories.By similarity

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni214 – 24936RNA-bindingSequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the rotavirus NSP2 family.Curated

Sequencei

Sequence statusi: Complete.

Q86197-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTQSVSLSDF IVKTEDGYMP SDRECIALDR YLSKEQKELR ETFKDGKNDR
60 70 80 90 100
AALRIKMFLC PSPSRRFTQH GVVPMREIKT NTDMPSTLWT LVTDWLLNLL
110 120 130 140 150
QDEENQEMFE DFISSKFPDV LASADKLARF AQRLEDRKDV LRKNFGKAMN
160 170 180 190 200
AFGACFWAIK PTFATEGKCN VVRASDDSII LEFQPVPEYF RCGKSKATFY
210 220 230 240 250
KLYPLSDEQP VNGMLALKAV AGNQFFMYHG HGHIRTVPYH ELLTLSNHSL
260 270
VKIKKRSKTF LNHHSQLNVV VNFSICSME
Length:279
Mass (Da):32,110
Last modified:November 1, 1996 - v1
Checksum:i9C48F27D0943BCA2
GO

Sequence cautioni

The sequence CAI30287.1 differs from that shown. Reason: Frameshift at position 243. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti60 – 601C → S in CAI30287 (Ref. 2) Curated
Sequence conflicti63 – 631P → L in CAI30287 (Ref. 2) Curated
Sequence conflicti84 – 841M → I in CAI30287 (Ref. 2) Curated
Sequence conflicti105 – 1051N → S in CAI30287 (Ref. 2) Curated
Sequence conflicti142 – 1421R → H in CAI30287 (Ref. 2) Curated
Sequence conflicti146 – 1461G → S in CAI30287 (Ref. 2) Curated
Sequence conflicti175 – 1751S → T in CAI30287 (Ref. 2) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M91437 mRNA. Translation: AAA47328.1.
AJ867609 Genomic RNA. Translation: CAI30287.1. Frameshift.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M91437 mRNA. Translation: AAA47328.1.
AJ867609 Genomic RNA. Translation: CAI30287.1. Frameshift.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

ProtoNetiSearch...

Publicationsi

  1. "The evolution of human group B rotaviruses: correction and an update."
    Jiang B., Wang Y., Glass R.I., Fang Z.-Y.
    J. Clin. Virol. 34:158-159(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Primary identification of the eighth RNA segment of the group B rotavirus ADRV."
    Mackow E.R., Chen G., Werner R., Fay M.E., Tao H.
    Submitted (SEP-1992) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiNSP2_ROTGA
AccessioniPrimary (citable) accession number: Q86197
Secondary accession number(s): Q5K038
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: November 1, 1996
Last modified: January 7, 2015
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.