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Q86197

- NSP2_ROTGA

UniProt

Q86197 - NSP2_ROTGA

Protein

Non-structural protein 2

Gene
N/A
Organism
Rotavirus B (isolate Human/China/ADRV/1982) (RV-B) (Rotavirus B (isolate adult diarrhea rotavirus))
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 39 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Involved in genome replication and packaging. Plays a crucial role, together with NSP5, in the formation of virus factories (viroplasms) which are large inclusions in the cytoplasm where replication intermediates are assembled and RNA replication takes place. Displays ssRNA binding, NTPase, RNA triphosphatase (RTPase) and ATP-independent helix-unwinding activity activities. The unwiding activity may prepare and organize plus-strand RNAs for packaging and replication by removing interfering secondary structures. Unlike typical helicases, NSP2 requires neither a divalent cation nor a nucleotide energy source for helix destabilization. The RTPase activity may account for the absence of the 5'-terminal gamma-phosphate on the minus-strands of dsRNA genome segments By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei233 – 2331For NTPase activityBy similarity

    GO - Molecular functioni

    1. hydrolase activity Source: UniProtKB-KW
    2. nucleotide binding Source: UniProtKB-KW
    3. RNA binding Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Nucleotide-binding, RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Non-structural protein 2 (EC:3.6.4.-)
    Short name:
    NSP2
    OrganismiRotavirus B (isolate Human/China/ADRV/1982) (RV-B) (Rotavirus B (isolate adult diarrhea rotavirus))
    Taxonomic identifieri10942 [NCBI]
    Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirus
    Virus hostiHomo sapiens (Human) [TaxID: 9606]

    Subcellular locationi

    Host cytoplasm Curated
    Note: Found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging.By similarity

    GO - Cellular componenti

    1. host cell cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 279279Non-structural protein 2PRO_0000369851Add
    BLAST

    Interactioni

    Subunit structurei

    Homooctamer. Interacts with NSP5; this interaction leads to formation of virus factories.By similarity

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni214 – 24936RNA-bindingSequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the rotavirus NSP2 family.Curated

    Sequencei

    Sequence statusi: Complete.

    Q86197-1 [UniParc]FASTAAdd to Basket

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    MTQSVSLSDF IVKTEDGYMP SDRECIALDR YLSKEQKELR ETFKDGKNDR    50
    AALRIKMFLC PSPSRRFTQH GVVPMREIKT NTDMPSTLWT LVTDWLLNLL 100
    QDEENQEMFE DFISSKFPDV LASADKLARF AQRLEDRKDV LRKNFGKAMN 150
    AFGACFWAIK PTFATEGKCN VVRASDDSII LEFQPVPEYF RCGKSKATFY 200
    KLYPLSDEQP VNGMLALKAV AGNQFFMYHG HGHIRTVPYH ELLTLSNHSL 250
    VKIKKRSKTF LNHHSQLNVV VNFSICSME 279
    Length:279
    Mass (Da):32,110
    Last modified:November 1, 1996 - v1
    Checksum:i9C48F27D0943BCA2
    GO

    Sequence cautioni

    The sequence CAI30287.1 differs from that shown. Reason: Frameshift at position 243.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti60 – 601C → S in CAI30287. 1 PublicationCurated
    Sequence conflicti63 – 631P → L in CAI30287. 1 PublicationCurated
    Sequence conflicti84 – 841M → I in CAI30287. 1 PublicationCurated
    Sequence conflicti105 – 1051N → S in CAI30287. 1 PublicationCurated
    Sequence conflicti142 – 1421R → H in CAI30287. 1 PublicationCurated
    Sequence conflicti146 – 1461G → S in CAI30287. 1 PublicationCurated
    Sequence conflicti175 – 1751S → T in CAI30287. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M91437 mRNA. Translation: AAA47328.1.
    AJ867609 Genomic RNA. Translation: CAI30287.1. Frameshift.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M91437 mRNA. Translation: AAA47328.1 .
    AJ867609 Genomic RNA. Translation: CAI30287.1 . Frameshift.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    ProtoNeti Search...

    Publicationsi

    1. "The evolution of human group B rotaviruses: correction and an update."
      Jiang B., Wang Y., Glass R.I., Fang Z.-Y.
      J. Clin. Virol. 34:158-159(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    2. "Primary identification of the eighth RNA segment of the group B rotavirus ADRV."
      Mackow E.R., Chen G., Werner R., Fay M.E., Tao H.
      Submitted (SEP-1992) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].

    Entry informationi

    Entry nameiNSP2_ROTGA
    AccessioniPrimary (citable) accession number: Q86197
    Secondary accession number(s): Q5K038
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 14, 2009
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 39 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3