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Q86197 (NSP2_ROTGA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 38. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Non-structural protein 2

Short name=NSP2
EC=3.6.4.-
OrganismRotavirus B (isolate Human/China/ADRV/1982) (RV-B) (Rotavirus B (isolate adult diarrhea rotavirus))
Taxonomic identifier10942 [NCBI]
Taxonomic lineageVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirus
Virus hostHomo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length279 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Involved in genome replication and packaging. Plays a crucial role, together with NSP5, in the formation of virus factories (viroplasms) which are large inclusions in the cytoplasm where replication intermediates are assembled and RNA replication takes place. Displays ssRNA binding, NTPase, RNA triphosphatase (RTPase) and ATP-independent helix-unwinding activity activities. The unwiding activity may prepare and organize plus-strand RNAs for packaging and replication by removing interfering secondary structures. Unlike typical helicases, NSP2 requires neither a divalent cation nor a nucleotide energy source for helix destabilization. The RTPase activity may account for the absence of the 5'-terminal gamma-phosphate on the minus-strands of dsRNA genome segments By similarity.

Subunit structure

Homooctamer By similarity. Interacts with NSP5; this interaction leads to formation of virus factories By similarity.

Subcellular location

Host cytoplasm Potential. Note: Found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging By similarity.

Sequence similarities

Belongs to the rotavirus NSP2 family.

Sequence caution

The sequence CAI30287.1 differs from that shown. Reason: Frameshift at position 243.

Ontologies

Keywords
   Cellular componentHost cytoplasm
   LigandNucleotide-binding
RNA-binding
   Molecular functionHydrolase
Gene Ontology (GO)
   Cellular_componenthost cell cytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionRNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

hydrolase activity

Inferred from electronic annotation. Source: UniProtKB-KW

nucleotide binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 279279Non-structural protein 2
PRO_0000369851

Regions

Region214 – 24936RNA-binding Potential

Sites

Active site2331For NTPase activity By similarity

Experimental info

Sequence conflict601C → S in CAI30287. Ref.2
Sequence conflict631P → L in CAI30287. Ref.2
Sequence conflict841M → I in CAI30287. Ref.2
Sequence conflict1051N → S in CAI30287. Ref.2
Sequence conflict1421R → H in CAI30287. Ref.2
Sequence conflict1461G → S in CAI30287. Ref.2
Sequence conflict1751S → T in CAI30287. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q86197 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 9C48F27D0943BCA2

FASTA27932,110
        10         20         30         40         50         60 
MTQSVSLSDF IVKTEDGYMP SDRECIALDR YLSKEQKELR ETFKDGKNDR AALRIKMFLC 

        70         80         90        100        110        120 
PSPSRRFTQH GVVPMREIKT NTDMPSTLWT LVTDWLLNLL QDEENQEMFE DFISSKFPDV 

       130        140        150        160        170        180 
LASADKLARF AQRLEDRKDV LRKNFGKAMN AFGACFWAIK PTFATEGKCN VVRASDDSII 

       190        200        210        220        230        240 
LEFQPVPEYF RCGKSKATFY KLYPLSDEQP VNGMLALKAV AGNQFFMYHG HGHIRTVPYH 

       250        260        270 
ELLTLSNHSL VKIKKRSKTF LNHHSQLNVV VNFSICSME 

« Hide

References

[1]"The evolution of human group B rotaviruses: correction and an update."
Jiang B., Wang Y., Glass R.I., Fang Z.-Y.
J. Clin. Virol. 34:158-159(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]"Primary identification of the eighth RNA segment of the group B rotavirus ADRV."
Mackow E.R., Chen G., Werner R., Fay M.E., Tao H.
Submitted (SEP-1992) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M91437 mRNA. Translation: AAA47328.1.
AJ867609 Genomic RNA. Translation: CAI30287.1. Frameshift.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

ProtoNetSearch...

Entry information

Entry nameNSP2_ROTGA
AccessionPrimary (citable) accession number: Q86197
Secondary accession number(s): Q5K038
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families