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Q86197

- NSP2_ROTGA

UniProt

Q86197 - NSP2_ROTGA

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Protein

Non-structural protein 2

Gene
N/A
Organism
Rotavirus B (isolate Human/China/ADRV/1982) (RV-B) (Rotavirus B (isolate adult diarrhea rotavirus))
Status
Reviewed - Annotation score: 2 out of 5 - Experimental evidence at transcript leveli

Functioni

Involved in genome replication and packaging. Plays a crucial role, together with NSP5, in the formation of virus factories (viroplasms) which are large inclusions in the cytoplasm where replication intermediates are assembled and RNA replication takes place. Displays ssRNA binding, NTPase, RNA triphosphatase (RTPase) and ATP-independent helix-unwinding activity activities. The unwiding activity may prepare and organize plus-strand RNAs for packaging and replication by removing interfering secondary structures. Unlike typical helicases, NSP2 requires neither a divalent cation nor a nucleotide energy source for helix destabilization. The RTPase activity may account for the absence of the 5'-terminal gamma-phosphate on the minus-strands of dsRNA genome segments By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei233 – 2331For NTPase activity By similarity

GO - Molecular functioni

  1. hydrolase activity Source: UniProtKB-KW
  2. nucleotide binding Source: UniProtKB-KW
  3. RNA binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Non-structural protein 2 (EC:3.6.4.-)
Short name:
NSP2
OrganismiRotavirus B (isolate Human/China/ADRV/1982) (RV-B) (Rotavirus B (isolate adult diarrhea rotavirus))
Taxonomic identifieri10942 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]

Subcellular locationi

Host cytoplasm Reviewed prediction
Note: Found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging By similarity.

GO - Cellular componenti

  1. host cell cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 279279Non-structural protein 2PRO_0000369851Add
BLAST

Interactioni

Subunit structurei

Homooctamer By similarity. Interacts with NSP5; this interaction leads to formation of virus factories By similarity.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni214 – 24936RNA-binding Reviewed predictionAdd
BLAST

Sequence similaritiesi

Belongs to the rotavirus NSP2 family.

Sequencei

Sequence statusi: Complete.

Q86197-1 [UniParc]FASTAAdd to Basket

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MTQSVSLSDF IVKTEDGYMP SDRECIALDR YLSKEQKELR ETFKDGKNDR    50
AALRIKMFLC PSPSRRFTQH GVVPMREIKT NTDMPSTLWT LVTDWLLNLL 100
QDEENQEMFE DFISSKFPDV LASADKLARF AQRLEDRKDV LRKNFGKAMN 150
AFGACFWAIK PTFATEGKCN VVRASDDSII LEFQPVPEYF RCGKSKATFY 200
KLYPLSDEQP VNGMLALKAV AGNQFFMYHG HGHIRTVPYH ELLTLSNHSL 250
VKIKKRSKTF LNHHSQLNVV VNFSICSME 279
Length:279
Mass (Da):32,110
Last modified:November 1, 1996 - v1
Checksum:i9C48F27D0943BCA2
GO

Sequence cautioni

The sequence CAI30287.1 differs from that shown. Reason: Frameshift at position 243.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti60 – 601C → S in CAI30287. 1 Publication
Sequence conflicti63 – 631P → L in CAI30287. 1 Publication
Sequence conflicti84 – 841M → I in CAI30287. 1 Publication
Sequence conflicti105 – 1051N → S in CAI30287. 1 Publication
Sequence conflicti142 – 1421R → H in CAI30287. 1 Publication
Sequence conflicti146 – 1461G → S in CAI30287. 1 Publication
Sequence conflicti175 – 1751S → T in CAI30287. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M91437 mRNA. Translation: AAA47328.1.
AJ867609 Genomic RNA. Translation: CAI30287.1. Frameshift.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M91437 mRNA. Translation: AAA47328.1 .
AJ867609 Genomic RNA. Translation: CAI30287.1 . Frameshift.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

ProtoNeti Search...

Publicationsi

  1. "The evolution of human group B rotaviruses: correction and an update."
    Jiang B., Wang Y., Glass R.I., Fang Z.-Y.
    J. Clin. Virol. 34:158-159(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Primary identification of the eighth RNA segment of the group B rotavirus ADRV."
    Mackow E.R., Chen G., Werner R., Fay M.E., Tao H.
    Submitted (SEP-1992) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiNSP2_ROTGA
AccessioniPrimary (citable) accession number: Q86197
Secondary accession number(s): Q5K038
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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