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Q86195 (VP2_ROTGA) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 33. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Inner capsid protein VP2
OrganismRotavirus B (isolate Human/China/ADRV/1982) (RV-B) (Rotavirus B (isolate adult diarrhea rotavirus))
Taxonomic identifier10942 [NCBI]
Taxonomic lineageVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirus
Virus hostHomo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length933 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Inner capsid protein that self assembles to form an icosahedral capsid with a T=2 symmetry, which consists of 120 copies of VP2, with channels at each of its five-fold vertices. This capsid constitutes the innermost concentric layer of the viral mature particle. It encapsidates the polymerase VP1, the capping enzyme VP3 and the genomic dsRNA, thereby defining the core. The innermost VP2 capsid and the intermediate VP6 capsid remain intact following cell entry to protect the dsRNA from degradation and to prevent unfavorable antiviral responses in the host cell during all the replication cycle of the virus. Nacent transcripts are transcribed within the structural confines of this double-layered particle (DLP) and are extruded through the channels at the five-fold axes. VP2 is required for the replicase activity of VP1 polymerase. It probably plays a role in the coordination of packaging and genome replication by controlling the initiation of minus-strand synthesis. Binding to the polymerase VP1 presumably activates the autoinhibited VP1-RNA complex which will start the synthesis of the complementary minus-strand By similarity.

Subunit structure

Dimer Potential. Interacts with VP1. Interacts with VP3. Interacts with NSP5 By similarity.

Subcellular location

Virion Potential. Note: Inner capsid protein. Also found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging By similarity.

Sequence similarities

Belongs to the rotavirus VP2 family.

Ontologies

Keywords
   Cellular componentCapsid protein
Inner capsid protein
Virion
   LigandRNA-binding
Gene Ontology (GO)
   Cellular_componentviral inner capsid

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionRNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 933933Inner capsid protein VP2
PRO_0000369832

Regions

Region407 – 42721Hydrophobic

Sequences

Sequence LengthMass (Da)Tools
Q86195 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 3FCF4CE129F14341

FASTA933105,635
        10         20         30         40         50         60 
MDSTVLVESA KTNIHGVDSK AEKQTIFDQL ISDIKSQTDG QIPDEVLPDL QQLAEINGLT 

        70         80         90        100        110        120 
FEYKPKEKLS IMDHPDPTSV LSQDVFQIRT ILSKTLFVDV ENEDYSVYIP NDTAKLTPVL 

       130        140        150        160        170        180 
IDARPIQTYQ PKALMHKDTA ILPSHRDEIS DQYGTDEILF DSHMFNDISQ AQIRDFDTYI 

       190        200        210        220        230        240 
LDKSIQIQNT LPNLEFISSL EKEVNPFNIH NTLCLNFGQK EYYNIIADRT NLSFQQRRQS 

       250        260        270        280        290        300 
VQFDNVIVDG VARTARVSLR LHPFDSQLLD IVRFNVIQDQ PLADTLMEYQ LVAADGFVAT 

       310        320        330        340        350        360 
PKFRVDRDAR LIADVRSPVM ARLCELSPFF HRTRILSSMT DFTPLWKVNV FSSSIDNAKD 

       370        380        390        400        410        420 
AIYRMAEISF TVADATTSAL ASVNVASAQQ TLLTLLNLSL FRFEIDPTGS QSNFGSAVSA 

       430        440        450        460        470        480 
ALMLIVFPTD EQSMSNITFD NLCNLVFNEL IAWTVDRPTF VKRTGMTNAF EANVNIGGGN 

       490        500        510        520        530        540 
MTRDIIAYMR FVLLRRPWAV FQRTYDDRYV SDIMVPNIDE ANVNDQCYVA INNLFNGLIQ 

       550        560        570        580        590        600 
AAQRNPNPGR QIAATSFRKL LKSMKDSCCN RIIPLIRLLK YNIERIARVY RFFPYTADLV 

       610        620        630        640        650        660 
HVIPAFRDER LRVKVPVSGM LSIALGINKA PDSFDWYNLL KFADVVRTKN FADQSLESIM 

       670        680        690        700        710        720 
VHALIRNDIN PARSKKDYIQ QNIKPATNVV ASLSKLPSAT FTTILADRML NNEIRRTQSY 

       730        740        750        760        770        780 
VVTNRIRDAV RAAFEHVPTA EHGIAKGALL LPIPQNFQRS SVYVRKDNIL YDPPVGVDRF 

       790        800        810        820        830        840 
NLSDLLDGRF YQGLINRVQN MAPFVISGPL QVKPSDASAI ESVTSAYLTM SSPYDACVRP 

       850        860        870        880        890        900 
EDLRHNRVVH PPTVDYFSDA SITRPNTQFE QLMSKTSVFV IDAPRLIVQN DATVYTFDYK 

       910        920        930 
DIQLTTSVVD KLEFTSVKTP DVTLFNGMLV YED 

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References

[1]"Cloning sequencing and expression of the gene encoding the VP2 protein of the human group B rotavirus, ADRV."
Mackow E.R., Fay M.E., Shaw R., Tao H., Chen G.
Virology 201:162-168(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M91433 Genomic RNA. Translation: AAA47350.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

ProtoNetSearch...

Entry information

Entry nameVP2_ROTGA
AccessionPrimary (citable) accession number: Q86195
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: November 1, 1996
Last modified: October 16, 2013
This is version 33 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families