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Protein

Inner capsid protein VP2

Gene
N/A
Organism
Rotavirus B (isolate Rat/United States/IDIR/1984) (RV-B) (Rotavirus B (isolate infectious diarrhea of infant rats))
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Inner capsid protein that self assembles to form an icosahedral capsid with a T=2 symmetry, which consists of 120 copies of VP2, with channels at each of its five-fold vertices. This capsid constitutes the innermost concentric layer of the viral mature particle. It encapsidates the polymerase VP1, the capping enzyme VP3 and the genomic dsRNA, thereby defining the core. The innermost VP2 capsid and the intermediate VP6 capsid remain intact following cell entry to protect the dsRNA from degradation and to prevent unfavorable antiviral responses in the host cell during all the replication cycle of the virus. Nacent transcripts are transcribed within the structural confines of this double-layered particle (DLP) and are extruded through the channels at the five-fold axes. VP2 is required for the replicase activity of VP1 polymerase. It probably plays a role in the coordination of packaging and genome replication by controlling the initiation of minus-strand synthesis. Binding to the polymerase VP1 presumably activates the autoinhibited VP1-RNA complex which will start the synthesis of the complementary minus-strand (By similarity).By similarity

GO - Molecular functioni

  1. RNA binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Inner capsid protein VP2
OrganismiRotavirus B (isolate Rat/United States/IDIR/1984) (RV-B) (Rotavirus B (isolate infectious diarrhea of infant rats))
Taxonomic identifieri28877 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Rattus norvegicus (Rat) [TaxID: 10116]

Subcellular locationi

  1. Virion Curated

  2. Note: Inner capsid protein. Also found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging (By similarity).By similarity

GO - Cellular componenti

  1. viral inner capsid Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Inner capsid protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 934934Inner capsid protein VP2PRO_0000369833Add
BLAST

Interactioni

Subunit structurei

Dimer (Potential). Interacts with VP1. Interacts with VP3. Interacts with NSP5 (By similarity).By similarityCurated

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni407 – 42721HydrophobicAdd
BLAST

Sequence similaritiesi

Belongs to the rotavirus VP2 family.Curated

Sequencei

Sequence statusi: Complete.

Q86108-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDFPNLVLSA KNSIQNVDSK DEKQKIIDQL ISDIRGQNDG VIPDEVLSEI
60 70 80 90 100
QQVAEINGLS FNYEKPVKQE LLEQPDPTSV LSQEVFQIRT ILSKTLFVDV
110 120 130 140 150
EAEDYSVYIP DETSNLSPVQ IDARPIQTYQ PKALMYKDTA ILPSNRDEVS
160 170 180 190 200
DQYGADEILF DSHMFNDISQ AQIRDFETYV LDKAMQIQSS LPNLDFVSSL
210 220 230 240 250
DKEVNPFNVH NTLCLNFGQR EYYNIIADRT NQSFQQRRQS VQFDNVVVDG
260 270 280 290 300
VARRARVSLR LHPFDSQLLD IIRFNTIQDQ PLADTMAEYQ LVAADGFVAT
310 320 330 340 350
PRFRTDRDAR LIADVRSRVM ARLCELSPYF HRTRILSSMT DFNSLWKVNV
360 370 380 390 400
FSSSIDNAKD AIYRMAEISF TVADATTSAL SSVNIASAQQ TLLVLLNMSL
410 420 430 440 450
FRFEIEPVGS QSNFGAAVSA ALMLVVFPTD EASMSNVTFD NLCNLVFNEL
460 470 480 490 500
IAWTVDRPTF VKRTGMTNAF EANVNIGGGN MTRDITAYMR FVLLRRPWAV
510 520 530 540 550
FQRTYDDQYV ADIMVPNIDE ANVNDQSYMA INNLFSGLIQ AAQRNPNPGR
560 570 580 590 600
QIAATSFRKL LKSMKDSCCN RIMPLIRLMK YNVERIARIY RFFPYTADLV
610 620 630 640 650
QRIPAFRDER IRIKVPVSGM LSIALGINKS PDVFDWYNLL RFADVIRTKN
660 670 680 690 700
FAERQSLESI MVQALIRNDI NPARSRKEYI QQNIKPATNV VASITKVPSA
710 720 730 740 750
TFTTILSDRM LNNEIRRTQS YIVVNRIRDA VRAAFEHVPT AEHGIAKGAL
760 770 780 790 800
LLPYPQNFQR SSVYVRKDNI LYNAPVGVDR FSLDDLLNGR FYQGMVNRIQ
810 820 830 840 850
NMSPFVIAGP LQVRASNASA IDSVTSAYLT MSSPYDACVK PEDLRHNRIV
860 870 880 890 900
QPPIVDFFSD SSITRPNTQF EQLMSKTSVF VIDAPKLIVQ SDATVYNFDY
910 920 930
RDIQLTTSVV DKLEFTSVKT PDVTLFNGML VFED
Length:934
Mass (Da):106,015
Last modified:November 1, 1996 - v1
Checksum:iF61A623E0B4CA090
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00673 Genomic RNA. Translation: AAA17401.1.
PIRiA49808.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00673 Genomic RNA. Translation: AAA17401.1.
PIRiA49808.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

ProtoNetiSearch...

Publicationsi

  1. "Group B rotavirus VP2: sequence analysis, expression, and gene coding assignment."
    Lindsay D.A., Waggie K.S., Eiden J.J.
    Virology 199:141-150(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiVP2_ROTGI
AccessioniPrimary (citable) accession number: Q86108
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: November 1, 1996
Last modified: October 1, 2014
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.