Q85WB7 (GSA_BRANA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate-1-semialdehyde 2,1-aminomutase, chloroplastic
Short name=GSA
EC=5.4.3.8

Alternative name(s):
Glutamate-1-semialdehyde aminotransferase
Short name=GSA-AT

Gene names

Name:

GSA

Organism

Brassica napus (Rape)

Taxonomic identifier

3708 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Brassiceae › Brassica

Protein attributes

Sequence length	473 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	(S)-4-amino-5-oxopentanoate = 5-aminolevulinate.
Cofactor	Pyridoxal phosphate.
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from L-glutamyl-tRNA(Glu): step 2/2. Porphyrin biosynthesis; chlorophyll biosynthesis.
Subunit structure	Homodimer By similarity.
Subcellular location	Plastid › chloroplast.
Sequence similarities	Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily.

Ontologies

Keywords
Biological process	Chlorophyll biosynthesis Porphyrin biosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	Pyridoxal phosphate
Molecular function	Isomerase
Gene Ontology (GO)
Biological_process	chlorophyll biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway protoporphyrinogen IX biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	glutamate-1-semialdehyde 2,1-aminomutase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro transaminase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 37	37	Chloroplast By similarity
	Chain	38 – 473	436	Glutamate-1-semialdehyde 2,1-aminomutase, chloroplastic		PRO_0000041970
Amino acid modifications
	Modified residue	313	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q85WB7 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: C9B978159C951DCB	FASTA	473	50,252
	10 20 30 40 50 60 MSATLTGSGT ALGFSCSSKI SKRVSSSPST RCSIKMSVSV DEKKKSFTLQ KSEEAFNAAK 70 80 90 100 110 120 NLMPGGVNSP VRAFKSVGGQ PVLIDSVKGS KMWDIDGNEY IDYVGSWGPA IIGHADDEVL 130 140 150 160 170 180 AALAETMKKG TSFGAPCLLE NVLAEMVISA VPSIEMVRFV NSGTEACMGV LRLARAFTNK 190 200 210 220 230 240 EKFIKFEGCY HGHANAFLVK AGSGVATLGL PDSPGVPKAA TSDTLTAPYN DIEAVAKLFE 250 260 270 280 290 300 AHKGEISAVI LEPVVGNSGF ITPTPEFING LRQLTKDNGA LLIFDEVMTG FRLAYGGAQE 310 320 330 340 350 360 YFGITPDLTT LGKIIGGGLP VGAYGGRRDI MEMVAPAGPM YQAGTLSGNP LAMTAGIHTL 370 380 390 400 410 420 KRLKQPGTYE YLDKITKELT NGILEAGKKT GHPMCGGYIS GMFGFFFAEG PVYNFADAKK 430 440 450 460 470 SDTEKFGKFF RGMLEEGVYF APSQFEAGFT SLAHTSEDIQ FTISAAERVL GRI

References

[1]

"Chlorophyll reduction in the seed of Brassica napus with a glutamate 1-semialdehyde aminotransferase antisense gene." Tsang E.W.T., Yang J., Chang Q., Nowak G., Kolenovsky A., McGregor D.I., Keller W.A. Plant Mol. Biol. 51:191-201(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF364595 mRNA. Translation: AAO63782.1.
3D structure databases
ProteinModelPortal	Q85WB7.
SMR	Q85WB7. Positions 47-470.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00251; UER00317. UPA00668.
Family and domain databases
Gene3D	3.40.640.10. 1 hit. 3.90.1150.10. 2 hits.
InterPro	IPR004639. 4pyrrol_synth_GluAld_NH2Trfase. IPR005814. Aminotrans_3. IPR015424. PyrdxlP-dep_Trfase. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view]
PANTHER	PTHR11986. PTHR11986. 1 hit. PTHR11986:SF5. PTHR11986:SF5. 1 hit.
Pfam	PF00202. Aminotran_3. 1 hit. [Graphical view]
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
TIGRFAMs	TIGR00713. hemL. 1 hit.
PROSITE	PS00600. AA_TRANSFER_CLASS_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSA_BRANA

Accession

Primary (citable) accession number: Q85WB7

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2005
Last sequence update:	June 1, 2003
Last modified:	April 3, 2013
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program