Reviewed, UniProtKB/Swiss-Prot Q85FX6 (ACSF_CYAME)

Names and origin

Protein names

Recommended name:
    Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase
      Short name=Mg-protoporphyrin IX monomethyl ester oxidative cyclase
    EC=1.14.13.81

Gene names

Name:	acsF
Synonyms:	ycf59

Encoded on

Plastid; Chloroplast

Organism

Cyanidioschyzon merolae (Red alga)

Taxonomic identifier

45157 [NCBI]

Taxonomic lineage

Eukaryota › Rhodophyta › Bangiophyceae › Cyanidiales › Cyanidiaceae › Cyanidioschyzon

Protein attributes

Sequence length	335 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the formation of the isocyclic ring in chlorophyll biosynthesis. Mediates the cyclase reaction, which results in the formation of divinylprotochlorophyllide (Pchlide) characteristic of all chlorophylls from magnesium-protoporphyrin IX 13-monomethyl ester (MgPMME) By similarity.
Catalytic activity	Magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O(2) = 13(1)-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADP(+) + H(2)O. 13(1)-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O(2) = 13(1)-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADP(+) + 2 H(2)O. 13(1)-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O(2) = divinylprotochlorophyllide + NADP(+) + 2 H(2)O.
Cofactor	Iron By similarity.
Pathway	Porphyrin biosynthesis; chlorophyll biosynthesis (light-independent).
Subcellular location	Plastid › chloroplast.
Sequence similarities	Belongs to the acsF family.

Ontologies

Keywords
Biological process	Chlorophyll biosynthesis Photosynthesis
Cellular component	Chloroplast Plastid
Ligand	Iron Metal-binding NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	chlorophyll biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW photosynthesis Inferred from electronic annotation. Source: HAMAP
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	iron ion binding Inferred from electronic annotation. Source: HAMAP magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 335	335	Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase		PRO_0000217542

Sequences

	Sequence		Length	Mass (Da)
	Q85FX6-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: BE3A1610AABF2915	FASTA	335	40,024
	10 20 30 40 50 60 MQTAVQTQTT VQTLLTPRFY TTDFDQMAKY DISKNRAEIE AIVNEFRKDY NQTHFIRDDE 70 80 90 100 110 120 FDQVWTQMPE SKRQWFKEFL ERSCTAEFSG FLLYKELSRR LKDTNPLLAE GFGYMSRDEA 130 140 150 160 170 180 RHAGFLNKAM ADFGVSLDLG FLTKHRQYTF FAPKFIFYAT YLSEKIGYWR YITIYRHLEA 190 200 210 220 230 240 HPKWSVYPIF KFFEKWCQDE NRHGDFFAAI LKAQPKWITG WASRLWCRFF LLSVFVTMYL 250 260 270 280 290 300 NDLQRKDFYQ LIGLDARAFD WHVIRKTNES AARLFPVVLD VDHVDFVRLM DECACAYAHW 310 320 330 RETQSVLSLA KIFACLFKLY VIKPINTQAL WNTIK

References

[1]

"Complete sequence and analysis of the plastid genome of the unicellular red alga Cyanidioschyzon merolae." Ohta N., Matsuzaki M., Misumi O., Miyagishima S.-Y., Nozaki H., Tanaka K., Shin-i T., Kohara Y., Kuroiwa T. DNA Res. 10:67-77(2003) [PubMed: 12755171] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 10D.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB002583 Genomic DNA. Translation: BAC76217.1.
RefSeq	NP_849055.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	845087.
Family and domain databases
HAMAP	MF_01840. [Tree]
InterPro	IPR008434. AcsF. IPR003251. Rubrerythrin. [Graphical view]
Pfam	PF02915. Rubrerythrin. 1 hit. [Graphical view]
TIGRFAMs	TIGR02029. AcsF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ACSF_CYAME

Accession

Primary (citable) accession number: Q85FX6

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
Last sequence update:	June 1, 2003
Last modified:	November 25, 2008
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)