Q859P9 (Q859P9_BPN4) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names	Submitted name: Virion RNA polymerase EMBL AAO24831.2
Organism	Enterobacteria phage N4 (Bacteriophage N4) [Reference proteome]
Taxonomic identifier	10752 [NCBI]
Taxonomic lineage	Viruses › dsDNA viruses, no RNA stage › Caudovirales › Podoviridae › N4likevirus
Virus host	Escherichia coli [TaxID: 562]

Protein attributes

Sequence length	3500 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

Ontologies

Keywords
Ligand	GTP-binding PDB 3Q0A PDB 3Q22 PDB 3Q24 Manganese PDB 3Q23 Metal-binding PDB 3Q22 PDB 3Q23 Nucleotide-binding
Technical term	3D-structure PDB 3C46 PDB 3C2P PDB 3C3L PDB 2PO4 PDB 3Q0A PDB 3Q22 PDB 3Q24 PDB 3Q23 PDB 4FF1 PDB 4FF2 PDB 4FF3 PDB 4FF4 Complete proteome Reference proteome
Gene Ontology (GO)
Molecular_function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Nucleotide binding	1434 – 1437	4	GTP PDB 3Q0A PDB 3Q22 PDB 3Q24
	Nucleotide binding	1559 – 1561	3	GTP PDB 3Q22
	Nucleotide binding	1667 – 1668	2	GTP PDB 3Q22
Sites
	Metal binding	1556	1	Manganese PDB 3Q23
	Metal binding	1557	1	Manganese; via carbonyl oxygen PDB 3Q23
	Metal binding	1948	1	Manganese PDB 3Q23
	Binding site	1421	1	GTP PDB 3Q0A PDB 3Q22 PDB 3Q24
	Binding site	1609	1	GTP PDB 3Q22
	Binding site	1663	1	GTP PDB 3Q22
	Binding site	1675	1	GTP PDB 3Q22
	Binding site	1948	1	GTP PDB 3Q0A PDB 3Q22 PDB 3Q24

Sequences

	Sequence		Length	Mass (Da)
	Q859P9 [UniParc]. Last modified December 12, 2006. Version 2. Checksum: B4A52EAC2E28BBDB	FASTA	3,500	382,495
	10 20 30 40 50 60 MSVFDRLAGF ADSVTNAKQV DVSTATAQKK AEQGVTTPLV SPDAAYQMQA ARTGNVGANA 70 80 90 100 110 120 FEPGTVQSDF MNLTPMQIMN KYGVEQGLQL INARADAGNQ VFNDSVTTRT PGEELGDIAT 130 140 150 160 170 180 GVGLGFVNTL GGIGALGAGL LNDDAGAVVA QQLSKFNDAV HATQSQALQD KRKLFAARNL 190 200 210 220 230 240 MNEVESERQY QTDKKEGTND IVASLSKFGR DFVGSIENAA QTDSIISDGL AEGVGSLLGA 250 260 270 280 290 300 GPVLRGASLL GKAVVPANTL RSAALAGAID AGTGTQSLAR IASTVGRAAP GMVGVGAMEA 310 320 330 340 350 360 GGAYQQTADE IMKMSLKDLE KSPVYQQHIK DGMSPEQARR QTASETGLTA AAIQLPIAAA 370 380 390 400 410 420 TGPLVSRFEM APFRAGSLGA VGMNLARETV EEGVQGATGQ LAQNIAQQQN IDKNQDLLKG 430 440 450 460 470 480 VGTQAGLGAL YGFGSAGVVQ APAGAARLAG AATAPVLRTT MAGVKAAGSV AGKVVSPIKN 490 500 510 520 530 540 TLVARGERVM KQNEEASPVA DDYVAQAAQE AMAQAPEAEV TIRDAVEATD ATPEQKVAAH 550 560 570 580 590 600 QYVSDLMNAT RFNPENYQEA PEHIRNAVAG STDQVQVIQK LADLVNTLDE SNPQALMEAA 610 620 630 640 650 660 SYMYDAVSEF EQFINRDPAA LDSIPKDSPA IELLNRYTNL TANIQNTPKV IGALNVINRM 670 680 690 700 710 720 INESAQNGSL NVTEESSPQE MQNVALAAEV APEKLNPESV NVVLKHAADG RIKLNNRQIA 730 740 750 760 770 780 ALQNAAAILK GAREYDAEAA RLGLRPQDIV SKQIKTDESR TQEGQYSALQ HANRIRSAYN 790 800 810 820 830 840 SGNFELASAY LNDFMQFAQH MQNKVGALNE HLVTGNADKN KSVHYQALTA DREWVRSRTG 850 860 870 880 890 900 LGVNPYDTKS VKFAQQVALE AKTVADIANA LASAYPELKV SHIKVTPLDS RLNAPAAEVV 910 920 930 940 950 960 KAFRQGNRDV ASSQPKADSV NQVKETPVTK QEPVTSTVQT KTPVSESVKT EPTTKESSPQ 970 980 990 1000 1010 1020 AIKEPVNQSE KQDVNLTNED NIKQPTESVK ETETSTKEST VTEELKEGID AVYPSLVGTA 1030 1040 1050 1060 1070 1080 DSKAEGIKNY FKLSFTLPEE QKSRTVGSEA PLKDVAQALS SRARYELFTE KETANPAFNG 1090 1100 1110 1120 1130 1140 EVIKRYKELM EHGEGIADIL RSRLAKFLNT KDVGKRFAQG TEANRWVGGK LLNIVEQDGD 1150 1160 1170 1180 1190 1200 TFKYNEQLLQ TAVLAGLQWR LTATSNTAIK DAKDVAAITG IDQALLPEGL VEQFDTGMTL 1210 1220 1230 1240 1250 1260 TEAVSSLAQK IESYWGLSRN PNAPLGYTKG IPTAMAAEIL AAFVESTDVV ENIVDMSEID 1270 1280 1290 1300 1310 1320 PDNKKTIGLY TITELDSFDP INSFPTAIEE AVLVNPTEKM FFGDDIPPVA NTQLRNPAVR 1330 1340 1350 1360 1370 1380 NTPEQKAALK AEQATEFYVH TPMVQFYETL GKDRILELMG AGTLNKELLN DNHAKSLEGK 1390 1400 1410 1420 1430 1440 NRSVEDSYNQ LFSVIEQVRA QSEDISTVPI HYAYNMTRVG RMQMLGKYNP QSAKLVREAI 1450 1460 1470 1480 1490 1500 LPTKATLDLS NQNNEDFSAF QLGLAQALDI KVHTMTREVM SDELTKLLEG NLKPAIDMMV 1510 1520 1530 1540 1550 1560 EFNTTGSLPE NAVDVLNTAL GDRKSFVALM ALMEYSRYLV AEDKSAFVTP LYVEADGVTN 1570 1580 1590 1600 1610 1620 GPINAMMLMT GGLFTPDWIR NIAKGGLFIG SPNKTMNEHR STADNNDLYQ ASTNALMESL 1630 1640 1650 1660 1670 1680 GKLRSNYASN MPIQSQIDSL LSLMDLFLPD INLGENGALE LKRGIAKNPL TITIYGSGAR 1690 1700 1710 1720 1730 1740 GIAGKLVSSV TDAIYERMSD VLKARAKDPN ISAAMAMFGK QAASEAHAEE LLARFLKDME 1750 1760 1770 1780 1790 1800 TLTSTVPVKR KGVLELQSTG TGAKGKINPK TYTIKGEQLK ALQENMLHFF VEPLRNGITQ 1810 1820 1830 1840 1850 1860 TVGESLVYST EQLQKATQIQ SVVLEDMFKQ RVQEKLAEKA KDPTWKKGDF LTQKELNDIQ 1870 1880 1890 1900 1910 1920 ASLNNLAPMI ETGSQTFYIA GSENAEVANQ VLATNLDDRM RVPMSIYAPA QAGVAGIPFM 1930 1940 1950 1960 1970 1980 TIGTGDGMMM QTLSTMKGAP KNTLKIFDGM NIGLNDITDA SRKANEAVYT SWQGNPIKNV 1990 2000 2010 2020 2030 2040 YESYAKFMKN VDFSKLSPEA LEAIGKSALE YDQRENATVD DIANAASLIE RNLRNIALGV 2050 2060 2070 2080 2090 2100 DIRHKVLDKV NLSIDQMAAV GAPYQNNGKI DLSNMTPEQQ ADELNKLFRE ELEARKQKVA 2110 2120 2130 2140 2150 2160 KARAEVKEET VSEKEPVNPD FGMVGREHKA SGVRILSATA IRNLAKISNL PSTQAATLAE 2170 2180 2190 2200 2210 2220 IQKSLAAKDY KIIYGTPTQV AEYARQKNVT ELTSQEMEEA QAGNIYGWTN FDDKTIYLVS 2230 2240 2250 2260 2270 2280 PSMETLIHEL VHASTFEEVY SFYQGNEVSP TSKQAIENLE GLMEQFRSLD ISKDSPEMRE 2290 2300 2310 2320 2330 2340 AYADAIATIE GHLSNGFVDP AISKAAALNE FMAWGLANRA LAAKQKRTSS LVQMVKDVYQ 2350 2360 2370 2380 2390 2400 AIKKLIWGRK QAPALGEDMF SNLLFNSAIL MRSQPTTQAV AKDGTLFHSK AYGNNERLSQ 2410 2420 2430 2440 2450 2460 LNQTFDKLVT DYLRTDPVTE VERRGNVANA LMSATRLVRD VQSHGFNMTA QEQSVFQMVT 2470 2480 2490 2500 2510 2520 AALATEAAID PHAMARAQEL YTHVMKHLTV EHFMADPDST NPADRYYAQQ KYDTISGANL 2530 2540 2550 2560 2570 2580 VEVDAKGRTS LLPTFLGLAM VNEELRSIIK EMPVPKADKK LGNDIDTLLT NAGTQVMESL 2590 2600 2610 2620 2630 2640 NRRMAGDQKA TNVQDSIDAL SETIMAAALK RESFYDAVAT PTGNFIDRAN QYVTDSIERL 2650 2660 2670 2680 2690 2700 SETVIEKADK VIANPSNIAA KGVAHLAKLT AAIASEKQGE IVAQGVMTAM NQGKVWQPFH 2710 2720 2730 2740 2750 2760 DLVNDIVGRT KTNANVYDLI KLVKSQISQD RQQFREHLPT VIAGKFSRKL TDTEWSAMHT 2770 2780 2790 2800 2810 2820 GLGKTDLAVL RETMSMAEIR DLLSSSKKVK DEISTLEKEI QNQAGRNWNL VQKKSKQLAQ 2830 2840 2850 2860 2870 2880 YMIMGEVGNN LLRNAHAISR LLGERITNGP VADVAAIDKL ITLYSLELMN KSDRDLLSEL 2890 2900 2910 2920 2930 2940 AQSEVEGMEF SIAYMVGQRT EEMRKAKGDN RTLLNHFKGY IPVENQQGVN LIIADDKEFA 2950 2960 2970 2980 2990 3000 KLNSQSFTRI GTYQGSTGFR TGSKGYYFSP VAARAPYSQG ILQNVRNTAG GVDIGTGFTL 3010 3020 3030 3040 3050 3060 GTMVAGRITD KPTVERITKA LAKGERGREP LMPIYNSKGQ VVAYEQSVDP NMLKHLNQDN 3070 3080 3090 3100 3110 3120 HFAKMVGVWR GRQVEEAKAQ RFNDILIEQL HAMYEKDIKD SSANKSQYVN LLGKIDDPVL 3130 3140 3150 3160 3170 3180 ADAINLMNIE TRHKAEELFG KDELWVRRDM LNDALGYRAA SIGDVWTGNS RWSPSTLDTV 3190 3200 3210 3220 3230 3240 KKMFLGAFGN KAYHVVMNAE NTIQNLVKDA KTVIVVKSVV VPAVNFLANI YQMIGRGVPV 3250 3260 3270 3280 3290 3300 KDIAVNIPRK TSEINQYIKS RLRQIDAEAE LRAAEGNPNL VRKLKTEIQS ITDSHRRMSI 3310 3320 3330 3340 3350 3360 WPLIEAGEFS SIADAGISRD DLLVAEGKIH EYMEKLANKL PEKVRNAGRY ALIAKDTALF 3370 3380 3390 3400 3410 3420 QGIQKTVEYS DFIAKAIIYD DLVKRKKKSS SEALGQVTEE FINYDRLPGR FRGYMESMGL 3430 3440 3450 3460 3470 3480 MWFYNFKIRS IKVAMSMIRN NPVHSLIATV VPAPTMFGNV GLPIQDNMLT MLAEGRLDYS 3490 3500 LGFGQGLRAP TLNPWFNLTH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome sequence and analysis of bacteriophage N4." Hendrix R.W., Rothman-Denes L., Hatfull G.F., Lawrence J.G., Pedulla M. Submitted (NOV-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"Structural basis for DNA-hairpin promoter recognition by the bacteriophage N4 virion RNA polymerase." Gleghorn M.L., Davydova E.K., Rothman-Denes L.B., Murakami K.S. Mol. Cell 32:707-717(2008) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) OF 998-2102.
[3]	"X-ray crystal structure of the polymerase domain of the bacteriophage N4 virion RNA polymerase." Murakami K.S., Davydova E.K., Rothman-Denes L.B. Proc. Natl. Acad. Sci. U.S.A. 105:5046-5051(2008) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) OF 998-2101.
[4]	"X-ray crystal structures elucidate the nucleotidyl transfer reaction of transcript initiation using two nucleotides." Gleghorn M.L., Davydova E.K., Basu R., Rothman-Denes L.B., Murakami K.S. Proc. Natl. Acad. Sci. U.S.A. 108:3566-3571(2011) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS) OF 998-2103 IN COMPLEX WITH GTP AND MANGANESE.
[5]	"Watching the Bacteriophage N4 RNA Polymerase Transcription by Time-dependent Soak-trigger-freeze X-ray Crystallography." Basu R.S., Murakami K.S. J. Biol. Chem. 288:3305-3311(2013) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) OF 998-2103.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

EF056009 Genomic DNA. Translation: AAO24831.2.

RefSeq

YP_950528.1. NC_008720.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2PO4	X-ray	2.00	A	998-2101	[»]
3C2P	X-ray	2.00	A/B	998-2102	[»]
3C3L	X-ray	2.40	A/B	998-2102	[»]
3C46	X-ray	2.00	A/B	998-2102	[»]
3Q0A	X-ray	2.69	A/B	998-2103	[»]
3Q22	X-ray	2.11	A/B	998-2103	[»]
3Q23	X-ray	1.80	A/B	998-2103	[»]
3Q24	X-ray	1.81	A/B	998-2102	[»]
4FF1	X-ray	2.47	A/B	998-2103	[»]
4FF2	X-ray	2.00	A/B	998-2103	[»]
4FF3	X-ray	2.00	A/B	998-2103	[»]
4FF4	X-ray	2.03	A/B	998-2103	[»]

ProteinModelPortal

Q859P9.

SMR

Q859P9. Positions 1008-2101.

ModBase

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Protocols and materials databases

StructuralBiologyKnowledgebase

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Genome annotation databases

GeneID

5075715.

Phylogenomic databases

ProtClustDB

CLSP2513194.

Family and domain databases

ProtoNet

Search...

Other

EvolutionaryTrace

Q859P9.

Entry information

Entry name

Q859P9_BPN4

Accession

Primary (citable) accession number: Q859P9

Entry history

Integrated into UniProtKB/TrEMBL:	June 1, 2003
Last sequence update:	December 12, 2006
Last modified:	May 1, 2013
This is version 45 of the entry and version 2 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)