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Q859P9

- Q859P9_BPN4

UniProt

Q859P9 - Q859P9_BPN4

Protein
Submitted name:

Virion RNA polymerase

Gene
N/A
Organism
Enterobacteria phage N4 (Bacteriophage N4)
Status
Unreviewed - Annotation score: 1 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 54 (01 Oct 2014)
      Sequence version 2 (12 Dec 2006)
      Previous versions | rss
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    Functioni

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei1421 – 14211AMPImported
    Binding sitei1421 – 14211ATPImported
    Binding sitei1421 – 14211GTPImported
    Metal bindingi1556 – 15561ManganeseImported
    Metal bindingi1557 – 15571Manganese; via carbonyl oxygenImported
    Binding sitei1561 – 15611AMP; via amide nitrogenImported
    Binding sitei1609 – 16091ATPImported
    Binding sitei1609 – 16091GTPImported
    Binding sitei1663 – 16631ATPImported
    Binding sitei1663 – 16631GTPImported
    Binding sitei1667 – 16671ATPImported
    Binding sitei1675 – 16751AMPImported
    Binding sitei1675 – 16751ATPImported
    Binding sitei1675 – 16751GTPImported
    Metal bindingi1948 – 19481ManganeseImported
    Binding sitei1948 – 19481AMPImported
    Binding sitei1948 – 19481ATPImported
    Binding sitei1948 – 19481GTPImported

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi1434 – 14374GTPImported
    Nucleotide bindingi1556 – 15616ATPImported
    Nucleotide bindingi1559 – 15613GTPImported
    Nucleotide bindingi1667 – 16682GTPImported

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. GTP binding Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW

    Keywords - Ligandi

    ATP-bindingImported, GTP-bindingImported, ManganeseImported, Metal-bindingImported, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Submitted name:
    Virion RNA polymeraseImported
    OrganismiEnterobacteria phage N4 (Bacteriophage N4)Imported
    Taxonomic identifieri10752 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesPodoviridaeN4likevirus
    Virus hostiEscherichia coli [TaxID: 562]
    ProteomesiUP000001789: Genome

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2PO4X-ray2.00A998-2101[»]
    3C2PX-ray2.00A/B998-2102[»]
    3C3LX-ray2.40A/B998-2102[»]
    3C46X-ray2.00A/B998-2102[»]
    3Q0AX-ray2.69A/B998-2103[»]
    3Q22X-ray2.11A/B998-2103[»]
    3Q23X-ray1.80A/B998-2103[»]
    3Q24X-ray1.81A/B998-2102[»]
    4FF1X-ray2.47A/B998-2103[»]
    4FF2X-ray2.00A/B998-2103[»]
    4FF3X-ray2.00A/B998-2103[»]
    4FF4X-ray2.03A/B998-2103[»]
    ProteinModelPortaliQ859P9.
    SMRiQ859P9. Positions 1008-2101.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ859P9.

    Sequencei

    Sequence statusi: Complete.

    Q859P9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSVFDRLAGF ADSVTNAKQV DVSTATAQKK AEQGVTTPLV SPDAAYQMQA     50
    ARTGNVGANA FEPGTVQSDF MNLTPMQIMN KYGVEQGLQL INARADAGNQ 100
    VFNDSVTTRT PGEELGDIAT GVGLGFVNTL GGIGALGAGL LNDDAGAVVA 150
    QQLSKFNDAV HATQSQALQD KRKLFAARNL MNEVESERQY QTDKKEGTND 200
    IVASLSKFGR DFVGSIENAA QTDSIISDGL AEGVGSLLGA GPVLRGASLL 250
    GKAVVPANTL RSAALAGAID AGTGTQSLAR IASTVGRAAP GMVGVGAMEA 300
    GGAYQQTADE IMKMSLKDLE KSPVYQQHIK DGMSPEQARR QTASETGLTA 350
    AAIQLPIAAA TGPLVSRFEM APFRAGSLGA VGMNLARETV EEGVQGATGQ 400
    LAQNIAQQQN IDKNQDLLKG VGTQAGLGAL YGFGSAGVVQ APAGAARLAG 450
    AATAPVLRTT MAGVKAAGSV AGKVVSPIKN TLVARGERVM KQNEEASPVA 500
    DDYVAQAAQE AMAQAPEAEV TIRDAVEATD ATPEQKVAAH QYVSDLMNAT 550
    RFNPENYQEA PEHIRNAVAG STDQVQVIQK LADLVNTLDE SNPQALMEAA 600
    SYMYDAVSEF EQFINRDPAA LDSIPKDSPA IELLNRYTNL TANIQNTPKV 650
    IGALNVINRM INESAQNGSL NVTEESSPQE MQNVALAAEV APEKLNPESV 700
    NVVLKHAADG RIKLNNRQIA ALQNAAAILK GAREYDAEAA RLGLRPQDIV 750
    SKQIKTDESR TQEGQYSALQ HANRIRSAYN SGNFELASAY LNDFMQFAQH 800
    MQNKVGALNE HLVTGNADKN KSVHYQALTA DREWVRSRTG LGVNPYDTKS 850
    VKFAQQVALE AKTVADIANA LASAYPELKV SHIKVTPLDS RLNAPAAEVV 900
    KAFRQGNRDV ASSQPKADSV NQVKETPVTK QEPVTSTVQT KTPVSESVKT 950
    EPTTKESSPQ AIKEPVNQSE KQDVNLTNED NIKQPTESVK ETETSTKEST 1000
    VTEELKEGID AVYPSLVGTA DSKAEGIKNY FKLSFTLPEE QKSRTVGSEA 1050
    PLKDVAQALS SRARYELFTE KETANPAFNG EVIKRYKELM EHGEGIADIL 1100
    RSRLAKFLNT KDVGKRFAQG TEANRWVGGK LLNIVEQDGD TFKYNEQLLQ 1150
    TAVLAGLQWR LTATSNTAIK DAKDVAAITG IDQALLPEGL VEQFDTGMTL 1200
    TEAVSSLAQK IESYWGLSRN PNAPLGYTKG IPTAMAAEIL AAFVESTDVV 1250
    ENIVDMSEID PDNKKTIGLY TITELDSFDP INSFPTAIEE AVLVNPTEKM 1300
    FFGDDIPPVA NTQLRNPAVR NTPEQKAALK AEQATEFYVH TPMVQFYETL 1350
    GKDRILELMG AGTLNKELLN DNHAKSLEGK NRSVEDSYNQ LFSVIEQVRA 1400
    QSEDISTVPI HYAYNMTRVG RMQMLGKYNP QSAKLVREAI LPTKATLDLS 1450
    NQNNEDFSAF QLGLAQALDI KVHTMTREVM SDELTKLLEG NLKPAIDMMV 1500
    EFNTTGSLPE NAVDVLNTAL GDRKSFVALM ALMEYSRYLV AEDKSAFVTP 1550
    LYVEADGVTN GPINAMMLMT GGLFTPDWIR NIAKGGLFIG SPNKTMNEHR 1600
    STADNNDLYQ ASTNALMESL GKLRSNYASN MPIQSQIDSL LSLMDLFLPD 1650
    INLGENGALE LKRGIAKNPL TITIYGSGAR GIAGKLVSSV TDAIYERMSD 1700
    VLKARAKDPN ISAAMAMFGK QAASEAHAEE LLARFLKDME TLTSTVPVKR 1750
    KGVLELQSTG TGAKGKINPK TYTIKGEQLK ALQENMLHFF VEPLRNGITQ 1800
    TVGESLVYST EQLQKATQIQ SVVLEDMFKQ RVQEKLAEKA KDPTWKKGDF 1850
    LTQKELNDIQ ASLNNLAPMI ETGSQTFYIA GSENAEVANQ VLATNLDDRM 1900
    RVPMSIYAPA QAGVAGIPFM TIGTGDGMMM QTLSTMKGAP KNTLKIFDGM 1950
    NIGLNDITDA SRKANEAVYT SWQGNPIKNV YESYAKFMKN VDFSKLSPEA 2000
    LEAIGKSALE YDQRENATVD DIANAASLIE RNLRNIALGV DIRHKVLDKV 2050
    NLSIDQMAAV GAPYQNNGKI DLSNMTPEQQ ADELNKLFRE ELEARKQKVA 2100
    KARAEVKEET VSEKEPVNPD FGMVGREHKA SGVRILSATA IRNLAKISNL 2150
    PSTQAATLAE IQKSLAAKDY KIIYGTPTQV AEYARQKNVT ELTSQEMEEA 2200
    QAGNIYGWTN FDDKTIYLVS PSMETLIHEL VHASTFEEVY SFYQGNEVSP 2250
    TSKQAIENLE GLMEQFRSLD ISKDSPEMRE AYADAIATIE GHLSNGFVDP 2300
    AISKAAALNE FMAWGLANRA LAAKQKRTSS LVQMVKDVYQ AIKKLIWGRK 2350
    QAPALGEDMF SNLLFNSAIL MRSQPTTQAV AKDGTLFHSK AYGNNERLSQ 2400
    LNQTFDKLVT DYLRTDPVTE VERRGNVANA LMSATRLVRD VQSHGFNMTA 2450
    QEQSVFQMVT AALATEAAID PHAMARAQEL YTHVMKHLTV EHFMADPDST 2500
    NPADRYYAQQ KYDTISGANL VEVDAKGRTS LLPTFLGLAM VNEELRSIIK 2550
    EMPVPKADKK LGNDIDTLLT NAGTQVMESL NRRMAGDQKA TNVQDSIDAL 2600
    SETIMAAALK RESFYDAVAT PTGNFIDRAN QYVTDSIERL SETVIEKADK 2650
    VIANPSNIAA KGVAHLAKLT AAIASEKQGE IVAQGVMTAM NQGKVWQPFH 2700
    DLVNDIVGRT KTNANVYDLI KLVKSQISQD RQQFREHLPT VIAGKFSRKL 2750
    TDTEWSAMHT GLGKTDLAVL RETMSMAEIR DLLSSSKKVK DEISTLEKEI 2800
    QNQAGRNWNL VQKKSKQLAQ YMIMGEVGNN LLRNAHAISR LLGERITNGP 2850
    VADVAAIDKL ITLYSLELMN KSDRDLLSEL AQSEVEGMEF SIAYMVGQRT 2900
    EEMRKAKGDN RTLLNHFKGY IPVENQQGVN LIIADDKEFA KLNSQSFTRI 2950
    GTYQGSTGFR TGSKGYYFSP VAARAPYSQG ILQNVRNTAG GVDIGTGFTL 3000
    GTMVAGRITD KPTVERITKA LAKGERGREP LMPIYNSKGQ VVAYEQSVDP 3050
    NMLKHLNQDN HFAKMVGVWR GRQVEEAKAQ RFNDILIEQL HAMYEKDIKD 3100
    SSANKSQYVN LLGKIDDPVL ADAINLMNIE TRHKAEELFG KDELWVRRDM 3150
    LNDALGYRAA SIGDVWTGNS RWSPSTLDTV KKMFLGAFGN KAYHVVMNAE 3200
    NTIQNLVKDA KTVIVVKSVV VPAVNFLANI YQMIGRGVPV KDIAVNIPRK 3250
    TSEINQYIKS RLRQIDAEAE LRAAEGNPNL VRKLKTEIQS ITDSHRRMSI 3300
    WPLIEAGEFS SIADAGISRD DLLVAEGKIH EYMEKLANKL PEKVRNAGRY 3350
    ALIAKDTALF QGIQKTVEYS DFIAKAIIYD DLVKRKKKSS SEALGQVTEE 3400
    FINYDRLPGR FRGYMESMGL MWFYNFKIRS IKVAMSMIRN NPVHSLIATV 3450
    VPAPTMFGNV GLPIQDNMLT MLAEGRLDYS LGFGQGLRAP TLNPWFNLTH 3500
    Length:3,500
    Mass (Da):382,495
    Last modified:December 12, 2006 - v2
    Checksum:iB4A52EAC2E28BBDB
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EF056009 Genomic DNA. Translation: AAO24831.2.
    RefSeqiYP_950528.1. NC_008720.1.

    Genome annotation databases

    GeneIDi5075715.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EF056009 Genomic DNA. Translation: AAO24831.2 .
    RefSeqi YP_950528.1. NC_008720.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2PO4 X-ray 2.00 A 998-2101 [» ]
    3C2P X-ray 2.00 A/B 998-2102 [» ]
    3C3L X-ray 2.40 A/B 998-2102 [» ]
    3C46 X-ray 2.00 A/B 998-2102 [» ]
    3Q0A X-ray 2.69 A/B 998-2103 [» ]
    3Q22 X-ray 2.11 A/B 998-2103 [» ]
    3Q23 X-ray 1.80 A/B 998-2103 [» ]
    3Q24 X-ray 1.81 A/B 998-2102 [» ]
    4FF1 X-ray 2.47 A/B 998-2103 [» ]
    4FF2 X-ray 2.00 A/B 998-2103 [» ]
    4FF3 X-ray 2.00 A/B 998-2103 [» ]
    4FF4 X-ray 2.03 A/B 998-2103 [» ]
    ProteinModelPortali Q859P9.
    SMRi Q859P9. Positions 1008-2101.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 5075715.

    Miscellaneous databases

    EvolutionaryTracei Q859P9.

    Family and domain databases

    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence and analysis of bacteriophage N4."
      Hendrix R.W., Rothman-Denes L., Hatfull G.F., Lawrence J.G., Pedulla M.
      Submitted (NOV-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    2. "Structural basis for DNA-hairpin promoter recognition by the bacteriophage N4 virion RNA polymerase."
      Gleghorn M.L., Davydova E.K., Rothman-Denes L.B., Murakami K.S.
      Mol. Cell 32:707-717(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) OF 998-2102.
    3. "X-ray crystal structure of the polymerase domain of the bacteriophage N4 virion RNA polymerase."
      Murakami K.S., Davydova E.K., Rothman-Denes L.B.
      Proc. Natl. Acad. Sci. U.S.A. 105:5046-5051(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) OF 998-2101.
    4. "X-ray crystal structures elucidate the nucleotidyl transfer reaction of transcript initiation using two nucleotides."
      Gleghorn M.L., Davydova E.K., Basu R., Rothman-Denes L.B., Murakami K.S.
      Proc. Natl. Acad. Sci. U.S.A. 108:3566-3571(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS) OF 998-2103 IN COMPLEX WITH GTP AND MANGANESE.
    5. "Watching the bacteriophage N4 RNA polymerase transcription by time-dependent soak-trigger-freeze X-ray crystallography."
      Basu R.S., Murakami K.S.
      J. Biol. Chem. 288:3305-3311(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) OF 998-2103 IN COMPLEX WITH AMP; ATP AND MANGANESE.

    Entry informationi

    Entry nameiQ859P9_BPN4
    AccessioniPrimary (citable) accession number: Q859P9
    Entry historyi
    Integrated into UniProtKB/TrEMBL: June 1, 2003
    Last sequence update: December 12, 2006
    Last modified: October 1, 2014
    This is version 54 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    3D-structureImported, Complete proteome, Reference proteomeImported

    External Data

    Dasty 3