Q852S5 (NDK2_TOBAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nucleoside diphosphate kinase 2, chloroplastic
EC=2.7.4.6

Alternative name(s):
Nucleoside diphosphate kinase II
Short name=NDK II
Short name=NDP kinase II
Short name=NDPK II

Gene names

Name:

NDPK2

Organism

Nicotiana tabacum (Common tobacco)

Taxonomic identifier

4097 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Nicotianoideae › Nicotianeae › Nicotiana

Protein attributes

Sequence length	232 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate.
Catalytic activity	ATP + nucleoside diphosphate = ADP + nucleoside triphosphate.
Cofactor	Magnesium By similarity. UniProtKB P08879
Subcellular location	Plastid › chloroplast Potential.
Sequence similarities	Belongs to the NDK family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	CTP biosynthetic process Inferred from electronic annotation. Source: InterPro GTP biosynthetic process Inferred from electronic annotation. Source: InterPro UTP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside diphosphate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 79	79	Chloroplast Ref.2
	Chain	80 – 232	153	Nucleoside diphosphate kinase 2, chloroplastic Ref.2		PRO_0000257978
Sites
	Active site	198	1	Pros-phosphohistidine intermediate By similarity UniProtKB P08879
	Binding site	92	1	ATP By similarity UniProtKB P08879
	Binding site	140	1	ATP By similarity UniProtKB P08879
	Binding site	168	1	ATP By similarity UniProtKB P08879
	Binding site	174	1	ATP By similarity UniProtKB P08879
	Binding site	185	1	ATP By similarity UniProtKB P08879
	Binding site	195	1	ATP By similarity UniProtKB P08879

Sequences

	Sequence		Length	Mass (Da)
	Q852S5 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 1AE99FD93B6CBB5E	FASTA	232	25,342
	10 20 30 40 50 60 MGCLSVVGAS PCVSSSALSS PTSRLSCAPS CKLILNPIKK NHHLAAFQPA VHLFASNQSR 70 80 90 100 110 120 SHASKRNHTT RIFLPHLVAS MEEVEETYIM IKPDGVQRGL VGEIISRFEK KGFKLTGLKL 130 140 150 160 170 180 FHCPKELAEE HYKDLQSKPF FPKLIDYITS GPVVCMAWEG VGVVASARKL IGATNPLNAE 190 200 210 220 230 PGTIRGDLAV QTGRNVVHGS DSPDNGKREI ALWFGEGELC SWTPVQEPWL IE

References

[1]	"NDPK from aluminum-tolerant cultured tobacco cells." Yamamoto Y., Mitoh C. Submitted (JUL-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Samsun NN.
[2]	Yamamoto Y., Asakura Y., Yamada H., Matsumoto H. Submitted (OCT-2000) to UniProtKB Cited for: PROTEIN SEQUENCE OF 80-89. Strain: cv. Samsun.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB088360 mRNA. Translation: BAC55280.1.
3D structure databases
HSSP	HSSP built from PDB template 1S57 based on UniProtKB O64903.
ProteinModelPortal	Q852S5.
SMR	Q852S5. Positions 80-232.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001564. Nucleoside_diP_kinase. IPR023005. Nucleoside_diP_kinase_AS. [Graphical view]
Gene3D	G3DSA:3.30.70.141. NDK. 1 hit.
PANTHER	PTHR11349. Nuc_diP_kinase_core. 1 hit.
Pfam	PF00334. NDK. 1 hit. [Graphical view]
PRINTS	PR01243. NUCDPKINASE.
SMART	SM00562. NDK. 1 hit. [Graphical view]
SUPFAM	SSF54919. NDK. 1 hit.
PROSITE	PS00469. NDP_KINASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NDK2_TOBAC

Accession

Primary (citable) accession number: Q852S5
Secondary accession number(s): P82855

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	June 1, 2003
Last modified:	May 31, 2011
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program