Q85297 (DUSP_MYXVL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable dual specificity protein phosphatase H1 homolog
EC=3.1.3.-
EC=3.1.3.48

Gene names

Ordered Locus Names:	m069L
ORF Names:	I1L

Organism

Myxoma virus (strain Lausanne) (MYXV) [Reference proteome]

Taxonomic identifier

31530 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Poxviridae › Chordopoxvirinae › Leporipoxvirus › Myxoma virus

Virus host

Oryctolagus cuniculus (Rabbit) [TaxID: 9986]

Protein attributes

Sequence length	178 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Serine/Tyrosine phosphatase which down-regulates cellular antiviral response by dephosphorylating activated STAT1 and blocking interferon (IFN)-stimulated innate immune responses By similarity.
Catalytic activity	Protein tyrosine phosphate + H2O = protein tyrosine + phosphate. Protein serine phosphate + H2O = protein serine + phosphate.
Subunit structure	Homodimer By similarity.
Subcellular location	Virion By similarity. Host cytoplasm By similarity. Note: Approximately 200 molecules of H1 are packaged within the virion and are essential for the viability of the virus By similarity.
Induction	Expressed in the late phase of the viral replicative cycle.
Sequence similarities	Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.

Ontologies

Keywords
Biological process	Host-virus interaction Inhibition of host STAT1 by virus Inhibition of host innate immune response by virus Inhibition of host interferon signaling pathway by virus Viral immunoevasion
Cellular component	Host cytoplasm Virion
Developmental stage	Late protein
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	peptidyl-tyrosine dephosphorylation Inferred from electronic annotation. Source: GOC suppression by virus of host STAT1 activity Inferred from electronic annotation. Source: UniProtKB-KW suppression by virus of host type I interferon-mediated signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	host cell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell virion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	protein tyrosine phosphatase activity Inferred from electronic annotation. Source: EC protein tyrosine/serine/threonine phosphatase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 178	178	Probable dual specificity protein phosphatase H1 homolog		PRO_0000094871
Sites
	Active site	110	1	Phosphocysteine intermediate
Experimental info
	Mutagenesis	110	1	C → S: Complete loss of activity. Ref.1
	Sequence conflict	172 – 178	7	HKLKLFG → T in AAA66956. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q85297 [UniParc]. Last modified July 11, 2002. Version 2. Checksum: 318C99B3400A885F	FASTA	178	20,627
	10 20 30 40 50 60 MDKKSLYENV LLKSTGALPK ARVPTKMMRV TDYVYLGNYN DAKAAPTSGI GFKYILNLTT 70 80 90 100 110 120 EKYTIKNSSI TIIHMPLVDD EYTDLTKYFD YATTFLSNCE DKHYPVLVHC MAGVNRSGAI 130 140 150 160 170 IMAYLMSRKS KDIPAFMYFL YIYHSIREQR GAFLENPSFR RQIIEKYIIN EHKLKLFG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Myxoma virus and Shope fibroma virus encode dual-specificity tyrosine/serine phosphatases which are essential for virus viability." Mossman K., Ostergaard H., Upton C., McFadden G. Virology 206:572-582(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION, MUTAGENESIS OF CYS-110.
[2]	"The complete DNA sequence of myxoma virus." Cameron C., Hota-Mitchell S., Chen L., Barrett J.W., Cao J.-X., Macaulay C., Willer D.O., Evans D.H., McFadden G. Virology 264:298-318(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	L31960 Genomic DNA. Translation: AAA66956.1. AF170726 Genomic DNA. Translation: AAF14957.1.
RefSeq	NP_051783.1. NC_001132.2.
3D structure databases
ProteinModelPortal	Q85297.
SMR	Q85297. Positions 1-171.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	932088.
Phylogenomic databases
ProtClustDB	CLSP2509785.
Family and domain databases
InterPro	IPR000340. Dual-sp_phosphatase_cat-dom. IPR020422. Dual-sp_phosphatase_subgr_cat. IPR024950. DUSP. IPR000387. Tyr/Dual-sp_Pase. IPR016130. Tyr_Pase_AS. [Graphical view]
PANTHER	PTHR10159. PTHR10159. 1 hit.
Pfam	PF00782. DSPc. 1 hit. [Graphical view]
SMART	SM00195. DSPc. 1 hit. [Graphical view]
PROSITE	PS00383. TYR_PHOSPHATASE_1. 1 hit. PS50056. TYR_PHOSPHATASE_2. 1 hit. PS50054. TYR_PHOSPHATASE_DUAL. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DUSP_MYXVL

Accession

Primary (citable) accession number: Q85297
Secondary accession number(s): Q9Q8N1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2002
Last sequence update:	July 11, 2002
Last modified:	April 3, 2013
This is version 72 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program