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Protein

Probable dual specificity protein phosphatase H1 homolog

Gene

m069L

Organism
Myxoma virus (strain Lausanne) (MYXV)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Serine/Tyrosine phosphatase which down-regulates cellular antiviral response by dephosphorylating activated STAT1 and blocking interferon (IFN)-stimulated innate immune responses.By similarity

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation
Protein serine phosphate + H2O = protein serine + phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei110 – 1101Phosphocysteine intermediate

GO - Molecular functioni

  1. protein tyrosine/serine/threonine phosphatase activity Source: InterPro
  2. protein tyrosine phosphatase activity Source: UniProtKB-EC

GO - Biological processi

  1. suppression by virus of host STAT1 activity Source: UniProtKB-KW
  2. suppression by virus of host type I interferon-mediated signaling pathway Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Biological processi

Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host interferon signaling pathway by virus, Inhibition of host STAT1 by virus, Viral immunoevasion

Names & Taxonomyi

Protein namesi
Recommended name:
Probable dual specificity protein phosphatase H1 homolog (EC:3.1.3.-, EC:3.1.3.48)
Gene namesi
Ordered Locus Names:m069L
ORF Names:I1L
OrganismiMyxoma virus (strain Lausanne) (MYXV)
Taxonomic identifieri31530 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePoxviridaeChordopoxvirinaeLeporipoxvirus
Virus hostiOryctolagus cuniculus (Rabbit) [TaxID: 9986]
ProteomesiUP000000867 Componenti: Genome

Subcellular locationi

Virion By similarity. Host cytoplasm By similarity
Note: Approximately 200 molecules of H1 are packaged within the virion and are essential for the viability of the virus.By similarity

GO - Cellular componenti

  1. host cell cytoplasm Source: UniProtKB-SubCell
  2. virion Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Virion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi110 – 1101C → S: Complete loss of activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 178178Probable dual specificity protein phosphatase H1 homologPRO_0000094871Add
BLAST

Expressioni

Inductioni

Expressed in the late phase of the viral replicative cycle.

Keywords - Developmental stagei

Late protein

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ85297.
SMRiQ85297. Positions 1-171.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.90.190.10. 1 hit.
InterProiIPR000340. Dual-sp_phosphatase_cat-dom.
IPR020422. Dual-sp_phosphatase_subgr_cat.
IPR024950. DUSP.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
[Graphical view]
PANTHERiPTHR10159. PTHR10159. 1 hit.
PfamiPF00782. DSPc. 1 hit.
[Graphical view]
SMARTiSM00195. DSPc. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 1 hit.
PROSITEiPS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q85297-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDKKSLYENV LLKSTGALPK ARVPTKMMRV TDYVYLGNYN DAKAAPTSGI
60 70 80 90 100
GFKYILNLTT EKYTIKNSSI TIIHMPLVDD EYTDLTKYFD YATTFLSNCE
110 120 130 140 150
DKHYPVLVHC MAGVNRSGAI IMAYLMSRKS KDIPAFMYFL YIYHSIREQR
160 170
GAFLENPSFR RQIIEKYIIN EHKLKLFG
Length:178
Mass (Da):20,627
Last modified:July 10, 2002 - v2
Checksum:i318C99B3400A885F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti172 – 1787HKLKLFG → T in AAA66956 (PubMed:7831813).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L31960 Genomic DNA. Translation: AAA66956.1.
AF170726 Genomic DNA. Translation: AAF14957.1.
RefSeqiNP_051783.1. NC_001132.2.

Genome annotation databases

GeneIDi932088.
KEGGivg:932088.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L31960 Genomic DNA. Translation: AAA66956.1.
AF170726 Genomic DNA. Translation: AAF14957.1.
RefSeqiNP_051783.1. NC_001132.2.

3D structure databases

ProteinModelPortaliQ85297.
SMRiQ85297. Positions 1-171.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi932088.
KEGGivg:932088.

Family and domain databases

Gene3Di3.90.190.10. 1 hit.
InterProiIPR000340. Dual-sp_phosphatase_cat-dom.
IPR020422. Dual-sp_phosphatase_subgr_cat.
IPR024950. DUSP.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
[Graphical view]
PANTHERiPTHR10159. PTHR10159. 1 hit.
PfamiPF00782. DSPc. 1 hit.
[Graphical view]
SMARTiSM00195. DSPc. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 1 hit.
PROSITEiPS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Myxoma virus and Shope fibroma virus encode dual-specificity tyrosine/serine phosphatases which are essential for virus viability."
    Mossman K., Ostergaard H., Upton C., McFadden G.
    Virology 206:572-582(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION, MUTAGENESIS OF CYS-110.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiDUSP_MYXVL
AccessioniPrimary (citable) accession number: Q85297
Secondary accession number(s): Q9Q8N1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 10, 2002
Last sequence update: July 10, 2002
Last modified: March 31, 2015
This is version 80 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.