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Q85036

- RDRP_ROTPY

UniProt

Q85036 - RDRP_ROTPY

Protein

RNA-directed RNA polymerase

Gene
N/A
Organism
Rotavirus A (strain Pig/Mexico/YM/1983 G11-P9[7]-I5-R1-C1-M1-A8-N1-T1-E1-H1) (RV-A)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 45 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    RNA-directed RNA polymerase that is involved in both transcription and genome replication. Together with VP3 capping enzyme, forms an enzyme complex positioned near the channels situated at each of the five-fold vertices of the core. Following infection, the outermost layer of the virus is lost, leaving a double-layered particle (DLP) made up of the core and VP6 shell. VP1 then catalyzes the transcription of fully conservative plus-strand genomic RNAs that are extruded through the DLP's channels into the cytoplasm where they function as mRNAs for translation of viral proteins. One copy of each of the viral (+)RNAs is also recruited during core assembly, together with newly synthesized polymerase complexes and VP2. The polymerase of these novo-formed particles catalyzes the synthesis of complementary minus-strands leading to dsRNA formation. To do so, the polymerase specifically recognizes and binds 4 bases 5'-UGUG-3' in the conserved 3'-sequence of plus-strand RNA templates. VP2 presumably activates the autoinhibited VP1-RNA complex to coordinate packaging and genome replication. Once dsRNA synthesis is complete, the polymerase switches to the transcriptional mode, thus providing secondary transcription By similarity.PROSITE-ProRule annotation

    Catalytic activityi

    Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation

    Cofactori

    Magnesium.Curated

    GO - Molecular functioni

    1. nucleotide binding Source: UniProtKB-KW
    2. RNA binding Source: UniProtKB-KW
    3. RNA-directed RNA polymerase activity Source: UniProtKB-KW

    GO - Biological processi

    1. transcription, DNA-templated Source: InterPro
    2. viral genome replication Source: InterPro

    Keywords - Molecular functioni

    Nucleotidyltransferase, RNA-directed RNA polymerase, Transferase

    Keywords - Biological processi

    Viral RNA replication

    Keywords - Ligandi

    Magnesium, Nucleotide-binding, RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    RNA-directed RNA polymerase (EC:2.7.7.48)
    Alternative name(s):
    Protein VP1
    OrganismiRotavirus A (strain Pig/Mexico/YM/1983 G11-P9[7]-I5-R1-C1-M1-A8-N1-T1-E1-H1) (RV-A)
    Taxonomic identifieri10919 [NCBI]
    Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirus
    Virus hostiSus scrofa (Pig) [TaxID: 9823]

    Subcellular locationi

    Virion Curated
    Note: Attached inside the inner capsid as a minor component. Also found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging By similarity.By similarity

    GO - Cellular componenti

    1. virion Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10881088RNA-directed RNA polymerasePRO_0000368048Add
    BLAST

    Interactioni

    Subunit structurei

    Interacts with VP3 Potential. Interacts with VP2; this interaction activates VP1. Interacts with NSP5; this interaction is probably necessary for the formation of functional virus factories. Interacts with NSP2; this interaction is weak By similarity.By similarityCurated

    Structurei

    3D structure databases

    ProteinModelPortaliQ85036.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini501 – 687187RdRp catalyticPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 RdRp catalytic domain.PROSITE-ProRule annotation

    Family and domain databases

    InterProiIPR001795. RNA-dir_pol_luteovirus.
    IPR007097. RNA-dir_pol_reovirus.
    IPR022071. Rotavirus_VP1.
    [Graphical view]
    PfamiPF02123. RdRP_4. 1 hit.
    PF12289. Rotavirus_VP1. 1 hit.
    [Graphical view]
    PROSITEiPS50523. RDRP_DSRNA_REO. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q85036-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGKYNLILSE YLSFVYNSQS AVQIPIYYSS NSELEKRCID FHAKCVDNSK     50
    KGLSLSSLFE EYKDVIDNAT LLSILSYSYD KYNAVERKLI NYAKGKPLEA 100
    DLTANELDYE NNKITSELFK SAEEYTDSLM DPAILTSISS NLNAVMFWLE 150
    RHSNDVGDAN KVYRRRLDLF IIVASTINKY GVPRHNEKYR YEYEVMKDKP 200
    YYLVTWANSA IEMLMSVFSH EDYLIAKELI ILSYSNRSTL AKLVSSPMSI 250
    LVALIDINGT FITNEELELE FSDKYVKAIV PDQTFNELQE MIDNMKKAGL 300
    VDIPRMIQEW LVDCSLEKFT LMSKIYSWSF HVGFRKQKMI DAALDQLKTE 350
    YTEDVDNEMY NEYTMLIRDE IVKMLEVPVK HDDHLLRDSE LAGLLSMSSA 400
    SNGESRQLKF GRKTIFSTKK NMHVMDDIAH GRYTPGVIPP VNVDRPIPLG 450
    RRDVPGRRTR IIFILPYEYN SAQHAVVEKM LSYAKHTREY AEFYSQSNQL 500
    LSYGDVTRFL SSNSMVLYTD VSQWDSSQHN TQPFRKGIIM GLDMLANMTN 550
    DPKVVQTLNL YKQTQINLMD SYVQIPDGNV IKKIQYGAVA SGEKQTKAAN 600
    SIANLALIKT VLSRIANKYS FITKIIRVDG DDNYAVLQFN TDVTKQMVQE 650
    VSNDVRYIYS RMNAKVKALV STVGIEIAKR YIAGGKIFFR AGINLLNNEK 700
    RGQSTQWDQA AILYSNYIVN KLRGFDTDRE FILTKIIQMT SVAITGSLRL 750
    FPSERVLTTN STFKVFDSED FIIEYGTTDD EVYIQRAFMS LSSQKSGIAD 800
    EIASSQTFKN YVSKLSDQLL VSKNAIVSKG IAVTEKAKLN SYAPVYLEKR 850
    RAQISALLTM LQKPVSFKSN KITINDILRD IKPFFVTTEA KLPIQYRKFM 900
    PTLPDNVQYV IQCIGSRTYQ IEDSGSKSSI SKLISKYSVY KPSIEELYKV 950
    ISLREQEIQL YLVSLGVPPV DAGTYVGSRI YSQDKYKILE SYVYNLLSIN 1000
    YGCYQLFDFN SPDLEKLIRI PFKGKIPAVT FILHLYAKLE IINYAIKNKS 1050
    WISLFCNYPK SEMIKLWKKM WNITALRSPY TSANFFQD 1088
    Length:1,088
    Mass (Da):124,793
    Last modified:November 1, 1996 - v1
    Checksum:i73C5723B9EA74357
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X76486 mRNA. Translation: CAA54024.1.
    PIRiS39261.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X76486 mRNA. Translation: CAA54024.1 .
    PIRi S39261.

    3D structure databases

    ProteinModelPortali Q85036.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR001795. RNA-dir_pol_luteovirus.
    IPR007097. RNA-dir_pol_reovirus.
    IPR022071. Rotavirus_VP1.
    [Graphical view ]
    Pfami PF02123. RdRP_4. 1 hit.
    PF12289. Rotavirus_VP1. 1 hit.
    [Graphical view ]
    PROSITEi PS50523. RDRP_DSRNA_REO. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Amino acid sequence of the porcine rotavirus YM VP1 protein."
      Almanza L., Arias C.F., Lopez S.
      Res. Virol. 145:313-317(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].

    Entry informationi

    Entry nameiRDRP_ROTPY
    AccessioniPrimary (citable) accession number: Q85036
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 14, 2009
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 45 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3