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Q85014

- NSP3_ROTP5

UniProt

Q85014 - NSP3_ROTP5

Protein

Non-structural protein 3

Gene
N/A
Organism
Rotavirus A (strain Pig/United States/OSU/1977 G5-P9[7]-I5-R1-C1-M1-A1-N1-T1-E1-H1) (RV-A) (Rotavirus A (strain Ohio State University))
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 42 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Involved in translation of viral mRNAs and shutoff of host protein synthesis. It is unclear whether it is required for the translation of viral mRNAs as well. Functions similarly to, and competes with the cellular poly(A)-binding protein PABPC1. Binds the conserved sequence 'GACC' at the 3' end of viral mRNAs and allows circularization of viral mRNAs in translation. Interacts with ZC3H7B/RoXaN and with the eukaryotic translation initiation factor eIF4G, using the same region employed by PABP-C1. NSP3 thus displaces PABPC1 from eIF4G, inhibiting the translation of cellular poly(A) mRNAs. Also responsible for the nuclear relocalization of PABPC1 upon rotavirus infection By similarity.By similarity

    GO - Molecular functioni

    1. RNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. regulation of translation Source: UniProtKB-KW
    2. suppression by virus of host gene expression Source: UniProtKB-KW

    Keywords - Biological processi

    Eukaryotic host gene expression shutoff by virus, Eukaryotic host translation shutoff by virus, Host gene expression shutoff by virus, Host-virus interaction, Translation regulation

    Keywords - Ligandi

    RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Non-structural protein 3
    Short name:
    NSP3
    Alternative name(s):
    NCVP4
    Non-structural RNA-binding protein 34
    Short name:
    NS34
    OrganismiRotavirus A (strain Pig/United States/OSU/1977 G5-P9[7]-I5-R1-C1-M1-A1-N1-T1-E1-H1) (RV-A) (Rotavirus A (strain Ohio State University))
    Taxonomic identifieri10915 [NCBI]
    Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirus
    Virus hostiSus scrofa (Pig) [TaxID: 9823]

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 313313Non-structural protein 3PRO_0000369459Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer. Interacts (via the coiled-coil region) with host ZC3H7B (via LD motif). Interacts with host EIF4G1 By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ85014.
    SMRiQ85014. Positions 7-163, 206-312.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni4 – 149146RNA-bindingBy similarityAdd
    BLAST
    Regioni150 – 20657DimerizationBy similarityAdd
    BLAST
    Regioni170 – 23465Interaction with ZC3H7BBy similarityAdd
    BLAST
    Regioni208 – 313106Interaction with EIF4G1By similarityAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili166 – 23772Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the rotavirus A NSP3 family.Curated

    Keywords - Domaini

    Coiled coil

    Family and domain databases

    InterProiIPR002873. Rotavirus_NSP3.
    [Graphical view]
    PfamiPF01665. Rota_NSP3. 1 hit.
    [Graphical view]
    SUPFAMiSSF69903. SSF69903. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q85014-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLKMESTQQM VSSIINTSFE AAVVAATSTL ELMGIQYDYN EIFTRVKSKF    50
    DYVMDDSGVK NNLLGKAATI DQALNGKFGS TIRNRNWMTD SKTVAKLDED 100
    VNKLRMILSS KGIDQKMRVL NACFSVKRIP GKSSSVIKCT RLMKDKIERG 150
    EVEVDDSFVD EKMEIDTIDW KARYDQLEKR FESLKQRVNE KYNSWVQKSK 200
    ERNENMYSLQ NVISQQQNQI ADLQQYCNKL EADLQSKISS LVSSVEWYLR 250
    SMELSDDVKT DIEQQLNSID AINPINAIDD LESLIRNLIQ DYDRTFLMLK 300
    GLVRQCNYEC TYE 313
    Length:313
    Mass (Da):36,178
    Last modified:November 1, 1996 - v1
    Checksum:i2631D5F60911F74C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X81431 mRNA. Translation: CAA57190.1.
    PIRiS51753.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X81431 mRNA. Translation: CAA57190.1 .
    PIRi S51753.

    3D structure databases

    ProteinModelPortali Q85014.
    SMRi Q85014. Positions 7-163, 206-312.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR002873. Rotavirus_NSP3.
    [Graphical view ]
    Pfami PF01665. Rota_NSP3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF69903. SSF69903. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Comparative nucleotide and amino acid sequence analysis of the sequence-specific RNA-binding rotavirus nonstructural protein NSP3."
      Rao C.D., Das M., Ilango P., Lalwani R., Rao B.S., Gowda K.
      Virology 207:327-333(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].

    Entry informationi

    Entry nameiNSP3_ROTP5
    AccessioniPrimary (citable) accession number: Q85014
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 14, 2009
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 42 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3