Reviewed, UniProtKB/Swiss-Prot Q84ZX5 (ALL1_ARTVU)

Names and origin

Protein names	Recommended name:     Major pollen allergen Art v 1 Alternative name(s):     Defensin-like protein 1     Allergen=Art v 1
Organism	Artemisia vulgaris (Mugwort)
Taxonomic identifier	4220 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › campanulids › Asterales › Asteraceae › Asteroideae › Anthemideae › Artemisia

Protein attributes

Sequence length	132 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Subcellular location	Secreted By similarity.
Domain	Contains several extensin-like repeats in the C-terminal section.
Post-translational modification	Around 76% of the prolines are hydroxylated. O-glycosylated. O-linkage of 3 galactoses plus 9-16 or 21-23 arabinose residues attached on one or two hydroxyprolines. Ref.1
Allergenic properties	Causes an allergic reaction in human.
Miscellaneous	Post-translational modifications might be important in the formation of epitopes recognized by IgE antibodies.
Sequence similarities	In the N-terminal section; belongs to the DEFL family.

Ontologies

Keywords
Cellular component	Secreted
Disease	Allergen
Domain	Signal
PTM	Disulfide bond Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	defense response Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Ref.1
	Chain	25 – 132	108	Major pollen allergen Art v 1		PRO_0000007033
Regions
	Region	28 – 77	50	Defensin-like domain
	Compositional bias	81 – 129	49	Pro-rich
Amino acid modifications
	Disulfide bond	30 ↔ 77		By similarity
	Disulfide bond	41 ↔ 61		By similarity
	Disulfide bond	46 ↔ 71		By similarity
	Disulfide bond	50 ↔ 73		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q84ZX5-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 745249C89919F316	FASTA	132	13,404
	10 20 30 40 50 60 MAKCSYVFCA VLLIFIVAIG EMEAAGSKLC EKTSKTYSGK CDNKKCDKKC IEWEKAQHGA 70 80 90 100 110 120 CHKREAGKES CFCYFDCSKS PPGATPAPPG AAPPPAAGGS PSPPADGGSP PPPADGGSPP 130 VDGGSPPPPS TH

References

[1]

"Art v 1, the major allergen of mugwort pollen, is a modular glycoprotein with a defensin-like and a hydroxyproline-rich domain." Himly M., Jahn-Schmid B., Dedic A., Kelemen P., Wopfner N., Altmann F., van Ree R., Briza P., Richter K., Ebner C., Ferreira F. FASEB J. 17:106-108(2003) [PubMed: 12475905] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 25-39, CHARACTERIZATION, MASS SPECTROMETRY, GLYCOSYLATION. Tissue: Pollen.

[2]

"Optimization of codon usage is required for effective genetic immunization against Art v 1, the major allergen of mugwort pollen." Bauer R., Himly M., Dedic A., Ferreira F., Thalhamer J., Hartl A. Allergy 58:1003-1010(2003) [PubMed: 14510717] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Pollen.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF493943 mRNA. Translation: AAO24900.1.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR008176. Gamma-thionin. IPR003614. Knot1. [Graphical view]
Pfam	PF00304. Gamma-thionin. 1 hit. [Graphical view]
SMART	SM00505. Knot1. 1 hit. [Graphical view]
PROSITE	PS00940. GAMMA_THIONIN. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALL1_ARTVU

Accession

Primary (citable) accession number: Q84ZX5

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
Last sequence update:	June 1, 2003
Last modified:	November 24, 2009
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)