Q84ZX5 (ALL1_ARTVU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Major pollen allergen Art v 1 Alternative name(s): Defensin-like protein 1 Allergen=Art v 1
Organism	Artemisia vulgaris (Mugwort)
Taxonomic identifier	4220 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › campanulids › Asterales › Asteraceae › Asteroideae › Anthemideae › Artemisiinae › Artemisia

Protein attributes

Sequence length	132 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Secreted By similarity.
Domain	Contains several extensin-like repeats in the C-terminal section.
Post-translational modification	Around 76% of the prolines are hydroxylated. O-glycosylated. O-linkage of 3 galactoses plus 9-16 or 21-23 arabinose residues attached on one or two hydroxyprolines. Ref.1
Allergenic properties	Causes an allergic reaction in human.
Miscellaneous	Post-translational modifications might be important in the formation of epitopes recognized by IgE antibodies.
Sequence similarities	In the N-terminal section; belongs to the DEFL family.

Ontologies

Keywords
Cellular component	Secreted
Disease	Allergen
Domain	Signal
PTM	Disulfide bond Glycoprotein
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological_process	defense response Inferred from electronic annotation. Source: InterPro
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 24

24

Ref.1

Chain

25 – 132

108

Major pollen allergen Art v 1

PRO_0000007033

Regions

Region

28 – 77

50

Defensin-like domain

Compositional bias

81 – 129

49

Pro-rich

Amino acid modifications

Disulfide bond

30 ↔ 77

By similarity

Disulfide bond

41 ↔ 61

By similarity

Disulfide bond

46 ↔ 71

By similarity

Disulfide bond

50 ↔ 73

By similarity

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

132

Helix Strand Turn

Details...

Beta strand

28 – 36

9

Helix

44 – 53

10

Beta strand

57 – 64

8

Turn

65 – 67

3

Beta strand

68 – 79

12

Beta strand

89 – 91

3

Sequences

	Sequence		Length	Mass (Da)
	Q84ZX5 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 745249C89919F316	FASTA	132	13,404
	10 20 30 40 50 60 MAKCSYVFCA VLLIFIVAIG EMEAAGSKLC EKTSKTYSGK CDNKKCDKKC IEWEKAQHGA 70 80 90 100 110 120 CHKREAGKES CFCYFDCSKS PPGATPAPPG AAPPPAAGGS PSPPADGGSP PPPADGGSPP 130 VDGGSPPPPS TH

References

[1]	"Art v 1, the major allergen of mugwort pollen, is a modular glycoprotein with a defensin-like and a hydroxyproline-rich domain." Himly M., Jahn-Schmid B., Dedic A., Kelemen P., Wopfner N., Altmann F., van Ree R., Briza P., Richter K., Ebner C., Ferreira F. FASEB J. 17:106-108(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 25-39, CHARACTERIZATION, MASS SPECTROMETRY, GLYCOSYLATION. Tissue: Pollen.
[2]	"Optimization of codon usage is required for effective genetic immunization against Art v 1, the major allergen of mugwort pollen." Bauer R., Himly M., Dedic A., Ferreira F., Thalhamer J., Hartl A. Allergy 58:1003-1010(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Pollen.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF493943 mRNA. Translation: AAO24900.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2KPY	NMR	-	A	25-132	[»]

ProteinModelPortal

Q84ZX5.

SMR

Q84ZX5. Positions 31-77.

ModBase

Search...

Protein family/group databases

Allergome

3102. Art v 1.0101.
753. Art v 1.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

Gene3D

3.30.30.10. 1 hit.

InterPro

IPR008176. Gamma-thionin.
IPR003614. Scorpion_toxin-like.
[Graphical view]

Pfam

PF00304. Gamma-thionin. 1 hit.
[Graphical view]

SMART

SM00505. Knot1. 1 hit.
[Graphical view]

SUPFAM

SSF57095. SSF57095. 1 hit.

PROSITE

PS00940. GAMMA_THIONIN. False negative.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q84ZX5.

Entry information

Entry name

ALL1_ARTVU

Accession

Primary (citable) accession number: Q84ZX5

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
Last sequence update:	June 1, 2003
Last modified:	May 1, 2013
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program