Reviewed, UniProtKB/Swiss-Prot Q84ZN6 (CESA8_ORYSJ)

Names and origin

Protein names

Recommended name:
    Probable cellulose synthase A catalytic subunit 8 [UDP-forming]
    EC=2.4.1.12
Alternative name(s):
    OsCesA8

Gene names

Name:	CESA8
Ordered Locus Names:	Os07g0208500, LOC_Os07g10770
ORF Names:	OJ1136_A05.10, OJ1559_F09.120, OsJ_022567

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	1081 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Probable catalytic subunit of cellulose synthase terminal complexes ('rosettes'), required for beta-1,4-glucan microfibril crystallization, a major mechanism of the cell wall formation By similarity.
Catalytic activity	UDP-glucose + (1,4-beta-D-glucosyl)(n) = UDP + (1,4-beta-D-glucosyl)(n+1).
Cofactor	Binds 2 zinc ions per subunit By similarity.
Pathway	Glycan metabolism; plant cellulose biosynthesis.
Subcellular location	Cell membrane; Multi-pass membrane protein Probable.
Sequence similarities	Belongs to the glycosyltransferase 2 family. Plant cellulose synthase subfamily. Contains 1 RING-type zinc finger.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation Cellulose biosynthesis
Cellular component	Cell membrane Membrane
Domain	Coiled coil Transmembrane Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW cellulose biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cellulose synthase (UDP-forming) activity Inferred from electronic annotation. Source: EC protein binding Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1081	1081	Probable cellulose synthase A catalytic subunit 8 [UDP-forming]		PRO_0000319368
Regions
	Topological domain	1 – 277	277	Cytoplasmic Potential
	Transmembrane	278 – 298	21	Potential
	Topological domain	299 – 300	2	Extracellular Potential
	Transmembrane	301 – 321	21	Potential
	Topological domain	322 – 864	543	Cytoplasmic Potential
	Transmembrane	865 – 885	21	Potential
	Topological domain	886 – 890	5	Extracellular Potential
	Transmembrane	891 – 911	21	Potential
	Topological domain	912 – 926	15	Cytoplasmic Potential
	Transmembrane	927 – 947	21	Potential
	Topological domain	948 – 977	30	Extracellular Potential
	Transmembrane	978 – 998	21	Potential
	Topological domain	999 – 1009	11	Cytoplasmic Potential
	Transmembrane	1010 – 1030	21	Potential
	Topological domain	1031 – 1039	9	Extracellular Potential
	Transmembrane	1040 – 1060	21	Potential
	Topological domain	1061 – 1081	21	Cytoplasmic Potential
	Zinc finger	19 – 65	47	RING-type; degenerate
	Coiled coil	450 – 477	28	Potential
	Compositional bias	119 – 122	4	Poly-Gly
Sites
	Active site	396	1	Potential
	Active site	781	1	Potential
	Metal binding	19	1	Zinc 1 By similarity
	Metal binding	22	1	Zinc 1 By similarity
	Metal binding	38	1	Zinc 2 By similarity
	Metal binding	41	1	Zinc 2 By similarity
	Metal binding	46	1	Zinc 1 By similarity
	Metal binding	49	1	Zinc 1 By similarity
	Metal binding	61	1	Zinc 2 By similarity
	Metal binding	64	1	Zinc 2 By similarity
	Binding site	562	1	Substrate Potential
	Binding site	564	1	Substrate Potential
Amino acid modifications
	Glycosylation	954	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q84ZN6-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: BA17F2D40BFAA2EB	FASTA	1,081	120,794
	10 20 30 40 50 60 MDGDADAVKS GRHGSGQACQ ICGDGVGTTA EGDVFAACDV CGFPVCRPCY EYERKDGTQA 70 80 90 100 110 120 CPQCKTKYKR HKGSPAIRGE EGEDTDADDV SDYNYPASGS ADQKQKIADR MRSWRMNAGG 130 140 150 160 170 180 GGDVGRPKYD SGEIGLTKYD SGEIPRGYIP SVTNSQISGE IPGASPDHHM MSPTGNIGKR 190 200 210 220 230 240 APFPYVNHSP NPSREFSGSI GNVAWKERVD GWKLKQDKGA IPMTNGTSIA PSEGRGVGDI 250 260 270 280 290 300 DASTDYNMED ALLNDETRQP LSRKVPLPSS RINPYRMVIV LRLVVLSIFL HYRITNPVRN 310 320 330 340 350 360 AYPLWLLSVI CEIWFALSWI LDQFPKWFPI NRETYLDRLA LRYDREGEPS QLAAVDIFVS 370 380 390 400 410 420 TVDPMKEPPL VTANTVLSIL AVDYPVDKVS CYVSDDGAAM LTFDALAETS EFARKWVPFV 430 440 450 460 470 480 KKYNIEPRAP EWYFSQKIDY LKDKVHPSFV KDRRAMKREY EEFKVRINGL VAKAQKVPEE 490 500 510 520 530 540 GWIMQDGTPW PGNNTRDHPG MIQVFLGHSG GLDTEGNELP RLVYVSREKR PGFQHHKKAG 550 560 570 580 590 600 AMNALVRVSA VLTNGQYMLN LDCDHYINNS KALREAMCFL MDPNLGRSVC YVQFPQRFDG 610 620 630 640 650 660 IDRNDRYANR NTVFFDINLR GLDGIQGPVY VGTGCVFNRT ALYGYEPPIK QKKKGSFLSS 670 680 690 700 710 720 LCGGRKKASK SKKKSSDKKK SNKHVDSAVP VFNLEDIEEG VEGAGFDDEK SLLMSQMSLE 730 740 750 760 770 780 KRFGQSAAFV ASTLMEYGGV PQSATPESLL KEAIHVISCG YEDKTEWGTE IGWIYGSVTE 790 800 810 820 830 840 DILTGFKMHA RGWRSIYCMP KRPAFKGSAP INLSDRLNQV LRWALGSVEI LFSRHCPIWY 850 860 870 880 890 900 GYGGRLKFLE RFAYINTTIY PLTSIPLLIY CVLPAICLLT GKFIIPEISN FASIWFISLF 910 920 930 940 950 960 ISIFATGILE MRWSGVGIDE WWRNEQFWVI GGISAHLFAV FQGLLKVLAG IDTNFTVTSK 970 980 990 1000 1010 1020 ASDEDGDFAE LYMFKWTTLL IPPTTILIIN LVGVVAGISY AINSGYQSWG PLFGKLFFAF 1030 1040 1050 1060 1070 1080 WVIVHLYPFL KGLMGRQNRT PTIVVVWAIL LASIFSLLWV RIDPFTTRVT GPDTQTCGIN C

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"Curated genome annotation of Oryza sativa ssp. japonica and comparative genome analysis with Arabidopsis thaliana." The rice annotation project (RAP) Genome Res. 17:175-183(2007) [PubMed: 17210932] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[3]	"The genomes of Oryza sativa: a history of duplications." Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H. PLoS Biol. 3:266-281(2005) [PubMed: 15685292] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[4]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed: 12869764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP003748 Genomic DNA. Translation: BAD30175.1. AP003837 Genomic DNA. Translation: BAC57282.1. AP008213 Genomic DNA. Translation: BAF21076.1. CM000144 Genomic DNA. Translation: EAZ39084.1. AK072356 mRNA. No translation available.
RefSeq	NP_001059162.1.
UniGene	Os.10176
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT2. Glycosyltransferase Family 2.
Genome annotation databases
GeneID	4342699.
KEGG	osa:4342699.
Organism-specific databases
Gramene	Q84ZN6.
Family and domain databases
InterPro	IPR005150. Cellulose_synth. IPR001841. Znf_RING. IPR017907. Znf_RING_CS. [Graphical view]
Pfam	PF03552. Cellulose_synt. 1 hit. [Graphical view]
SMART	SM00184. RING. 1 hit. [Graphical view]
PROSITE	PS00518. ZF_RING_1. False negative. PS50089. ZF_RING_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CESA8_ORYSJ

Accession

Primary (citable) accession number: Q84ZN6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	June 1, 2003
Last modified:	June 16, 2009
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)