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Protein

Probable lipoxygenase 8, chloroplastic

Gene

CM-LOX2

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Plant lipoxygenase may be involved in a number of diverse aspects of plant physiology including growth and development, pest resistance, and senescence or responses to wounding. It catalyzes the hydroperoxidation of lipids containing a cis,cis-1,4-pentadiene structure (By similarity).By similarity

Catalytic activityi

Linoleate + O2 = (9Z,11E,13S)-13-hydroperoxyoctadeca-9,11-dienoate.
Alpha-linolenate + O2 = (9Z,11E,13S,15Z)-13-hydroperoxyoctadeca-9,11,15-trienoate.

Cofactori

Fe cationPROSITE-ProRule annotationNote: Binds 1 Fe cation per subunit. Iron is tightly bound.PROSITE-ProRule annotation

Pathwayi: oxylipin biosynthesis

This protein is involved in the pathway oxylipin biosynthesis, which is part of Lipid metabolism.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway oxylipin biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi598 – 5981Iron; catalyticPROSITE-ProRule annotation
Metal bindingi603 – 6031Iron; catalyticPROSITE-ProRule annotation
Metal bindingi790 – 7901Iron; catalyticPROSITE-ProRule annotation
Metal bindingi794 – 7941Iron; catalyticPROSITE-ProRule annotation
Metal bindingi941 – 9411Iron; via carboxylate; catalyticPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism, Oxylipin biosynthesis

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

ReactomeiR-OSA-2142691. Synthesis of Leukotrienes (LT) and Eoxins (EX).
R-OSA-2142696. Synthesis of Hepoxilins (HX) and Trioxilins (TrX).
R-OSA-2142700. Synthesis of Lipoxins (LX).
R-OSA-2142712. Synthesis of 12-eicosatetraenoic acid derivatives.
R-OSA-2142770. Synthesis of 15-eicosatetraenoic acid derivatives.
UniPathwayiUPA00382.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable lipoxygenase 8, chloroplastic (EC:1.13.11.12)
Gene namesi
Name:CM-LOX2
Ordered Locus Names:Os08g0509100, LOC_Os08g39850
ORF Names:B1168A08.28-1, B1168A08.28-2, OSJNBa0016N23.103-1, OSJNBa0016N23.103-2
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryza
Proteomesi
  • UP000059680 Componenti: Chromosome 8

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 6767ChloroplastSequence analysisAdd
BLAST
Chaini68 – 941874Probable lipoxygenase 8, chloroplasticPRO_0000018328Add
BLAST

Proteomic databases

PaxDbiQ84YK8.
PRIDEiQ84YK8.

Expressioni

Gene expression databases

ExpressionAtlasiQ84YK8. baseline and differential.
GenevisibleiQ84YK8. OS.

Interactioni

Protein-protein interaction databases

STRINGi39947.LOC_Os08g39850.1.

Structurei

3D structure databases

ProteinModelPortaliQ84YK8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini100 – 236137PLATPROSITE-ProRule annotationAdd
BLAST
Domaini242 – 941700LipoxygenasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the lipoxygenase family.Curated
Contains 1 lipoxygenase domain.PROSITE-ProRule annotation
Contains 1 PLAT domain.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiENOG410IIW1. Eukaryota.
ENOG410YN4N. LUCA.
HOGENOMiHOG000230469.
InParanoidiQ84YK8.
KOiK00454.
OMAiDHGVVTY.

Family and domain databases

Gene3Di2.60.60.20. 1 hit.
4.10.372.10. 1 hit.
InterProiIPR000907. LipOase.
IPR013819. LipOase_C.
IPR020834. LipOase_CS.
IPR020833. LipOase_Fe_BS.
IPR001246. LipOase_plant.
IPR027433. Lipoxygenase_domain_3.
IPR001024. PLAT/LH2_dom.
[Graphical view]
PANTHERiPTHR11771. PTHR11771. 2 hits.
PfamiPF00305. Lipoxygenase. 1 hit.
PF01477. PLAT. 1 hit.
[Graphical view]
PRINTSiPR00087. LIPOXYGENASE.
PR00468. PLTLPOXGNASE.
SMARTiSM00308. LH2. 1 hit.
[Graphical view]
SUPFAMiSSF48484. SSF48484. 1 hit.
SSF49723. SSF49723. 1 hit.
PROSITEiPS00711. LIPOXYGENASE_1. 1 hit.
PS00081. LIPOXYGENASE_2. 1 hit.
PS51393. LIPOXYGENASE_3. 1 hit.
PS50095. PLAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q84YK8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRPQLNPSS SHHHTTTTSS SSSTQLYFAS SSCIASLRRP SPPSLIAGAG
60 70 80 90 100
CRTTRRRQQG RQRVVVRCAS SSAASSASEA ARRGTGSSDM APAAVVKVKA
110 120 130 140 150
VATIKVTVEG LLNSLRPSKA IDNIRDLIGR SLFLELVSSE LEAKTGKKKA
160 170 180 190 200
TVHSYAHKVD DDDHGVVTYE ADFDVPTGFG PIGAVVVTNE LGQEMFLEDL
210 220 230 240 250
NLTAGDGAGN STVLPIRCNS WVQPKSSIDE GTPGKRIFFA KAYLPGQTPA
260 270 280 290 300
GLRSYREEDL KQKRGNGAGQ READDRVYDY DVYNDLGNPD SNGDLARPVL
310 320 330 340 350
GGSKQFPYPR RCRTGRPPSK KDPKSETRKG NVYVPRDEEF SEVKNAQFLL
360 370 380 390 400
KTLQSVLHAA VPAAQSALID NLSLNLPFPS FFVIDKLFED GVELPGVEKL
410 420 430 440 450
GFLHSIVPRL LELLRDSPGD KILLFDTPAN VQKDKFAWLR DEEFARETLA
460 470 480 490 500
GINPYAIELV REFPLKSKLD PAVYGPAESA ITADLLEEQM RRVMTVEEAI
510 520 530 540 550
SQKRLFMLDF HDLFLPYVHK IRSLKHTTMY GSRTIFFLTD DGTLRLLAIE
560 570 580 590 600
LTRPASPSQP QWRQVFTPST DTTKSWLWRM AKAHVRAHDA GHHELITHWL
610 620 630 640 650
RTHCAVEPYI IAANRQLSEM HPIYQLLHPH FRYTMRINAL ARSRLISAAG
660 670 680 690 700
IIELSFSPQK YSMELSSVAY DKLWRFDMEA LPADLVRRGM AEEDPTAEHG
710 720 730 740 750
LRLAIEDYPF ANDGLLIWDA IKTWVQAYVA RFYPDADSVA GDEELQAFWT
760 770 780 790 800
EVRTKGHGDK KDAPWWPKLD SPESLAHTLT TIVWVAAAHH AAVNFGQYDF
810 820 830 840 850
GGYFPNRPSI ARTVMPVEEP VDGAAMERFL DNPDQALREC FPSQVQATVV
860 870 880 890 900
MAVLDVLSTH STDEEYLGGE QTRPWNSDAA VQAAYAGFTA RLKEIEGVID
910 920 930 940
GRNKDRKLKN RCGAGILPYQ LMKPFSDAGV TGMGIPNSTS I
Length:941
Mass (Da):104,494
Last modified:June 1, 2003 - v1
Checksum:iBE3C7BA515137C32
GO

Sequence cautioni

The sequence BAD10669.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence BAD10729.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti590 – 5901A → T in AAD42043 (Ref. 5) Curated
Sequence conflicti639 – 6391A → T in AAD42043 (Ref. 5) Curated
Sequence conflicti711 – 7111A → T in AAD42043 (Ref. 5) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP005816 Genomic DNA. Translation: BAD10668.1.
AP005816 Genomic DNA. Translation: BAD10669.1. Sequence problems.
AP006049 Genomic DNA. Translation: BAC57390.1.
AP006049 Genomic DNA. Translation: BAD10729.1. Sequence problems.
AP008214 Genomic DNA. Translation: BAF24114.1.
AP014964 Genomic DNA. Translation: BAT06179.1.
AK066825 mRNA. Translation: BAG90142.1.
AF095896 mRNA. Translation: AAD42043.1.
RefSeqiXP_015650450.1. XM_015794964.1.
UniGeneiOs.13008.

Genome annotation databases

EnsemblPlantsiOS08T0509100-01; OS08T0509100-01; OS08G0509100.
GeneIDi4345994.
GrameneiOS08T0509100-01; OS08T0509100-01; OS08G0509100.
KEGGiosa:4345994.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP005816 Genomic DNA. Translation: BAD10668.1.
AP005816 Genomic DNA. Translation: BAD10669.1. Sequence problems.
AP006049 Genomic DNA. Translation: BAC57390.1.
AP006049 Genomic DNA. Translation: BAD10729.1. Sequence problems.
AP008214 Genomic DNA. Translation: BAF24114.1.
AP014964 Genomic DNA. Translation: BAT06179.1.
AK066825 mRNA. Translation: BAG90142.1.
AF095896 mRNA. Translation: AAD42043.1.
RefSeqiXP_015650450.1. XM_015794964.1.
UniGeneiOs.13008.

3D structure databases

ProteinModelPortaliQ84YK8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi39947.LOC_Os08g39850.1.

Proteomic databases

PaxDbiQ84YK8.
PRIDEiQ84YK8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiOS08T0509100-01; OS08T0509100-01; OS08G0509100.
GeneIDi4345994.
GrameneiOS08T0509100-01; OS08T0509100-01; OS08G0509100.
KEGGiosa:4345994.

Phylogenomic databases

eggNOGiENOG410IIW1. Eukaryota.
ENOG410YN4N. LUCA.
HOGENOMiHOG000230469.
InParanoidiQ84YK8.
KOiK00454.
OMAiDHGVVTY.

Enzyme and pathway databases

UniPathwayiUPA00382.
ReactomeiR-OSA-2142691. Synthesis of Leukotrienes (LT) and Eoxins (EX).
R-OSA-2142696. Synthesis of Hepoxilins (HX) and Trioxilins (TrX).
R-OSA-2142700. Synthesis of Lipoxins (LX).
R-OSA-2142712. Synthesis of 12-eicosatetraenoic acid derivatives.
R-OSA-2142770. Synthesis of 15-eicosatetraenoic acid derivatives.

Gene expression databases

ExpressionAtlasiQ84YK8. baseline and differential.
GenevisibleiQ84YK8. OS.

Family and domain databases

Gene3Di2.60.60.20. 1 hit.
4.10.372.10. 1 hit.
InterProiIPR000907. LipOase.
IPR013819. LipOase_C.
IPR020834. LipOase_CS.
IPR020833. LipOase_Fe_BS.
IPR001246. LipOase_plant.
IPR027433. Lipoxygenase_domain_3.
IPR001024. PLAT/LH2_dom.
[Graphical view]
PANTHERiPTHR11771. PTHR11771. 2 hits.
PfamiPF00305. Lipoxygenase. 1 hit.
PF01477. PLAT. 1 hit.
[Graphical view]
PRINTSiPR00087. LIPOXYGENASE.
PR00468. PLTLPOXGNASE.
SMARTiSM00308. LH2. 1 hit.
[Graphical view]
SUPFAMiSSF48484. SSF48484. 1 hit.
SSF49723. SSF49723. 1 hit.
PROSITEiPS00711. LIPOXYGENASE_1. 1 hit.
PS00081. LIPOXYGENASE_2. 1 hit.
PS51393. LIPOXYGENASE_3. 1 hit.
PS50095. PLAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  2. "The rice annotation project database (RAP-DB): 2008 update."
    The rice annotation project (RAP)
    Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: cv. Nipponbare.
  3. Cited for: GENOME REANNOTATION.
    Strain: cv. Nipponbare.
  4. "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
    The rice full-length cDNA consortium
    Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Nipponbare.
  5. "Purification and cDNA cloning of two rice lipoxygenases induced by blast fungus."
    Peng Y., Hou Z.
    Submitted (OCT-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 259-941.
    Strain: cv. Aichi asahi.
    Tissue: Seedling leaf.

Entry informationi

Entry nameiLOXC2_ORYSJ
AccessioniPrimary (citable) accession number: Q84YK8
Secondary accession number(s): Q0J4K1, Q7EXZ6, Q9XHM6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: June 1, 2003
Last modified: June 8, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.