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Protein

Probable lipoxygenase 8, chloroplastic

Gene

CM-LOX2

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Plant lipoxygenase may be involved in a number of diverse aspects of plant physiology including growth and development, pest resistance, and senescence or responses to wounding. It catalyzes the hydroperoxidation of lipids containing a cis,cis-1,4-pentadiene structure (By similarity).By similarity

Catalytic activityi

Linoleate + O2 = (9Z,11E,13S)-13-hydroperoxyoctadeca-9,11-dienoate.
Alpha-linolenate + O2 = (9Z,11E,13S,15Z)-13-hydroperoxyoctadeca-9,11,15-trienoate.

Cofactori

Fe cationPROSITE-ProRule annotationNote: Binds 1 Fe cation per subunit. Iron is tightly bound.PROSITE-ProRule annotation

Pathwayi: oxylipin biosynthesis

This protein is involved in the pathway oxylipin biosynthesis, which is part of Lipid metabolism.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway oxylipin biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi598Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi603Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi790Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi794Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi941Iron; via carboxylate; catalyticPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDioxygenase, Oxidoreductase
Biological processFatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism, Oxylipin biosynthesis
LigandIron, Metal-binding

Enzyme and pathway databases

ReactomeiR-OSA-2142691 Synthesis of Leukotrienes (LT) and Eoxins (EX)
R-OSA-2142696 Synthesis of Hepoxilins (HX) and Trioxilins (TrX)
R-OSA-2142700 Synthesis of Lipoxins (LX)
R-OSA-2142712 Synthesis of 12-eicosatetraenoic acid derivatives
R-OSA-2142770 Synthesis of 15-eicosatetraenoic acid derivatives
R-OSA-6798695 Neutrophil degranulation
UniPathwayiUPA00382

Names & Taxonomyi

Protein namesi
Recommended name:
Probable lipoxygenase 8, chloroplastic (EC:1.13.11.12)
Gene namesi
Name:CM-LOX2
Ordered Locus Names:Os08g0509100, LOC_Os08g39850
ORF Names:B1168A08.28-1, B1168A08.28-2, OSJNBa0016N23.103-1, OSJNBa0016N23.103-2
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryzaOryza sativa
Proteomesi
  • UP000059680 Componenti: Chromosome 8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 67ChloroplastSequence analysisAdd BLAST67
ChainiPRO_000001832868 – 941Probable lipoxygenase 8, chloroplasticAdd BLAST874

Proteomic databases

PaxDbiQ84YK8

Expressioni

Gene expression databases

ExpressionAtlasiQ84YK8 baseline and differential
GenevisibleiQ84YK8 OS

Interactioni

Protein-protein interaction databases

STRINGi39947.LOC_Os08g39850.1

Structurei

3D structure databases

ProteinModelPortaliQ84YK8
SMRiQ84YK8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini100 – 236PLATPROSITE-ProRule annotationAdd BLAST137
Domaini242 – 941LipoxygenasePROSITE-ProRule annotationAdd BLAST700

Sequence similaritiesi

Belongs to the lipoxygenase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiENOG410IIW1 Eukaryota
ENOG410YN4N LUCA
HOGENOMiHOG000230469
InParanoidiQ84YK8
KOiK00454
OMAiGVVTYEA
OrthoDBiEOG093601O5

Family and domain databases

Gene3Di4.10.372.10, 1 hit
InterProiView protein in InterPro
IPR000907 LipOase
IPR013819 LipOase_C
IPR036226 LipOase_C_sf
IPR020834 LipOase_CS
IPR020833 LipOase_Fe_BS
IPR001246 LipOase_plant
IPR027433 Lipoxygenase_dom_3
IPR001024 PLAT/LH2_dom
IPR036392 PLAT/LH2_dom_sf
PANTHERiPTHR11771 PTHR11771, 1 hit
PfamiView protein in Pfam
PF00305 Lipoxygenase, 1 hit
PF01477 PLAT, 1 hit
PRINTSiPR00087 LIPOXYGENASE
PR00468 PLTLPOXGNASE
SMARTiView protein in SMART
SM00308 LH2, 1 hit
SUPFAMiSSF48484 SSF48484, 1 hit
SSF49723 SSF49723, 1 hit
PROSITEiView protein in PROSITE
PS00711 LIPOXYGENASE_1, 1 hit
PS00081 LIPOXYGENASE_2, 1 hit
PS51393 LIPOXYGENASE_3, 1 hit
PS50095 PLAT, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q84YK8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRPQLNPSS SHHHTTTTSS SSSTQLYFAS SSCIASLRRP SPPSLIAGAG
60 70 80 90 100
CRTTRRRQQG RQRVVVRCAS SSAASSASEA ARRGTGSSDM APAAVVKVKA
110 120 130 140 150
VATIKVTVEG LLNSLRPSKA IDNIRDLIGR SLFLELVSSE LEAKTGKKKA
160 170 180 190 200
TVHSYAHKVD DDDHGVVTYE ADFDVPTGFG PIGAVVVTNE LGQEMFLEDL
210 220 230 240 250
NLTAGDGAGN STVLPIRCNS WVQPKSSIDE GTPGKRIFFA KAYLPGQTPA
260 270 280 290 300
GLRSYREEDL KQKRGNGAGQ READDRVYDY DVYNDLGNPD SNGDLARPVL
310 320 330 340 350
GGSKQFPYPR RCRTGRPPSK KDPKSETRKG NVYVPRDEEF SEVKNAQFLL
360 370 380 390 400
KTLQSVLHAA VPAAQSALID NLSLNLPFPS FFVIDKLFED GVELPGVEKL
410 420 430 440 450
GFLHSIVPRL LELLRDSPGD KILLFDTPAN VQKDKFAWLR DEEFARETLA
460 470 480 490 500
GINPYAIELV REFPLKSKLD PAVYGPAESA ITADLLEEQM RRVMTVEEAI
510 520 530 540 550
SQKRLFMLDF HDLFLPYVHK IRSLKHTTMY GSRTIFFLTD DGTLRLLAIE
560 570 580 590 600
LTRPASPSQP QWRQVFTPST DTTKSWLWRM AKAHVRAHDA GHHELITHWL
610 620 630 640 650
RTHCAVEPYI IAANRQLSEM HPIYQLLHPH FRYTMRINAL ARSRLISAAG
660 670 680 690 700
IIELSFSPQK YSMELSSVAY DKLWRFDMEA LPADLVRRGM AEEDPTAEHG
710 720 730 740 750
LRLAIEDYPF ANDGLLIWDA IKTWVQAYVA RFYPDADSVA GDEELQAFWT
760 770 780 790 800
EVRTKGHGDK KDAPWWPKLD SPESLAHTLT TIVWVAAAHH AAVNFGQYDF
810 820 830 840 850
GGYFPNRPSI ARTVMPVEEP VDGAAMERFL DNPDQALREC FPSQVQATVV
860 870 880 890 900
MAVLDVLSTH STDEEYLGGE QTRPWNSDAA VQAAYAGFTA RLKEIEGVID
910 920 930 940
GRNKDRKLKN RCGAGILPYQ LMKPFSDAGV TGMGIPNSTS I
Length:941
Mass (Da):104,494
Last modified:June 1, 2003 - v1
Checksum:iBE3C7BA515137C32
GO

Sequence cautioni

The sequence BAD10669 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence BAD10729 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti590A → T in AAD42043 (Ref. 5) Curated1
Sequence conflicti639A → T in AAD42043 (Ref. 5) Curated1
Sequence conflicti711A → T in AAD42043 (Ref. 5) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP005816 Genomic DNA Translation: BAD10668.1
AP005816 Genomic DNA Translation: BAD10669.1 Sequence problems.
AP006049 Genomic DNA Translation: BAC57390.1
AP006049 Genomic DNA Translation: BAD10729.1 Sequence problems.
AP008214 Genomic DNA Translation: BAF24114.1
AP014964 Genomic DNA Translation: BAT06179.1
AK066825 mRNA Translation: BAG90142.1
AF095896 mRNA Translation: AAD42043.1
RefSeqiXP_015650450.1, XM_015794964.1
UniGeneiOs.13008

Genome annotation databases

EnsemblPlantsiOs08t0509100-01; Os08t0509100-01; Os08g0509100
GeneIDi4345994
GrameneiOs08t0509100-01; Os08t0509100-01; Os08g0509100
KEGGiosa:4345994

Similar proteinsi

Entry informationi

Entry nameiLOXC2_ORYSJ
AccessioniPrimary (citable) accession number: Q84YK8
Secondary accession number(s): Q0J4K1, Q7EXZ6, Q9XHM6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: June 1, 2003
Last modified: March 28, 2018
This is version 98 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health