Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Monothiol glutaredoxin-S14, chloroplastic

Gene

GRXS14

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May only reduce GSH-thiol disulfides, but not protein disulfides (Potential). Probably involved in the regulation of the redox state of the BOLA proteins (Potential). May act as Fe-S cluster donors to Fe-S cluster-requiring proteins (PubMed:18044966). May protect cells against protein oxidative damage (PubMed:16829529). May regulate CAX cation transporters (PubMed:16829529). The GRXS14-BOLA1 heterodimer binds a labile, oxygen sensitive Fe-S cluster (PubMed:24714563).Curated3 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei89GlutathioneBy similarity1
Metal bindingi97Iron-sulfur (2Fe-2S); shared with dimeric partner2 Publications1
Metal bindingi99Iron-sulfur (2Fe-2S); shared with dimeric partner2 Publications1
Binding sitei126GlutathioneBy similarity1
Binding sitei130Glutathione1 Publication1
Binding sitei138Glutathione; via amide nitrogen and carbonyl oxygenBy similarity1

GO - Molecular functioni

GO - Biological processi

  • cation transport Source: TAIR
  • cell redox homeostasis Source: InterPro
Complete GO annotation...

Keywords - Ligandi

2Fe-2S, Iron, Iron-sulfur, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Monothiol glutaredoxin-S14, chloroplastic1 Publication
Short name:
AtGRXcp1 Publication
Short name:
AtGrxS141 Publication
Alternative name(s):
CAX-interacting protein 11 Publication
Short name:
CXIP11 Publication
Gene namesi
Name:GRXS141 Publication
Synonyms:CXIP11 Publication, GRX5P1 Publication, GRXCP1 Publication
Ordered Locus Names:At3g54900Imported
ORF Names:F28P10.120Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G54900.

Subcellular locationi

GO - Cellular componenti

  • chloroplast Source: TAIR
  • chloroplast envelope Source: TAIR
  • chloroplast stroma Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Disruption phenotypei

Plants display high sensitivity to external oxidants, and their content of protein carbonylation is higher than wild-type plants.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi97 – 100CGFS → AAAA: Loss of CAX1 activation. 1 Publication4
Mutagenesisi97C → A: Decreases protein stability. 1 Publication1
Mutagenesisi97C → S: No effect on interactions with BOLA proteins, but strongly reduced interaction with SUFE1. 1 Publication1
Mutagenesisi99F → A: No effect on protein stability. 1 Publication1
Mutagenesisi133 – 137SNWPT → AAAAA: Loss of CAX1 activation. 1 Publication5

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 63ChloroplastSequence analysisAdd BLAST63
ChainiPRO_000026873464 – 173Monothiol glutaredoxin-S14, chloroplasticAdd BLAST110

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei97S-glutathionyl cysteineBy similarity1
Disulfide bondi172Interchain1 Publication

Keywords - PTMi

Disulfide bond, Glutathionylation

Proteomic databases

PaxDbiQ84Y95.
PRIDEiQ84Y95.

Expressioni

Tissue specificityi

Hihgly expressed in leaves, at intermediate levels in stems and at lower levels in roots and flowers.2 Publications

Inductioni

By sodium and magnesium chloride.2 Publications

Gene expression databases

GenevisibleiQ84Y95. AT.

Interactioni

Subunit structurei

[2Fe-2S]-bridged holo-homodimer (By similarity). Interacts with N-terminal part of CAX1 in yeast (PubMed:12480930). Interacts in vitro with SUFE1, BOLA1, BOLA2 and BOLA4 (PubMed:24203231). Interacts in vivo only with SUFE1, BOLA1 and BOLA4 (PubMed:24203231, PubMed:24714563).By similarity3 Publications

Protein-protein interaction databases

BioGridi9971. 10 interactors.
IntActiQ84Y95. 4 interactors.
STRINGi3702.AT3G54900.1.

Structurei

Secondary structure

1173
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi69 – 79Combined sources11
Beta strandi81 – 90Combined sources10
Beta strandi92 – 97Combined sources6
Helixi98 – 109Combined sources12
Beta strandi115 – 118Combined sources4
Helixi119 – 121Combined sources3
Helixi123 – 133Combined sources11
Beta strandi136 – 138Combined sources3
Beta strandi140 – 143Combined sources4
Beta strandi146 – 149Combined sources4
Helixi151 – 160Combined sources10
Helixi162 – 172Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2MMANMR-A65-173[»]
3IPZX-ray2.40A65-173[»]
ProteinModelPortaliQ84Y95.
SMRiQ84Y95.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ84Y95.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini72 – 173GlutaredoxinPROSITE-ProRule annotationAdd BLAST102

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni97 – 100Required for CAX1 activation4
Regioni133 – 137Required for CAX1 activation5
Regioni151 – 152Glutathione bindingBy similarity2

Domaini

The C-terminal region (79-168) is involved in BOLA recognition in GRXS-BOLA apo-heterodimer.1 Publication

Sequence similaritiesi

Belongs to the glutaredoxin family. CGFS subfamily.Curated
Contains 1 glutaredoxin domain.PROSITE-ProRule annotation

Keywords - Domaini

Redox-active center, Transit peptide

Phylogenomic databases

eggNOGiKOG0911. Eukaryota.
COG0278. LUCA.
HOGENOMiHOG000095211.
InParanoidiQ84Y95.
OMAiEVEKAMC.
OrthoDBiEOG09360Q6L.
PhylomeDBiQ84Y95.

Family and domain databases

CDDicd03028. GRX_PICOT_like. 1 hit.
Gene3Di3.40.30.10. 1 hit.
InterProiIPR002109. Glutaredoxin.
IPR033658. GRX_PICOT-like.
IPR004480. Monothiol_GRX-rel.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PANTHERiPTHR10293. PTHR10293. 1 hit.
PfamiPF00462. Glutaredoxin. 1 hit.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 1 hit.
TIGRFAMsiTIGR00365. TIGR00365. 1 hit.
PROSITEiPS51354. GLUTAREDOXIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q84Y95-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALRSVKTPT LITSVAVVSS SVTNKPHSIR FSLKPTSALV VHNHQLSFYG
60 70 80 90 100
SNLKLKPTKF RCSASALTPQ LKDTLEKLVN SEKVVLFMKG TRDFPMCGFS
110 120 130 140 150
NTVVQILKNL NVPFEDVNIL ENEMLRQGLK EYSNWPTFPQ LYIGGEFFGG
160 170
CDITLEAFKT GELQEEVEKA MCS
Length:173
Mass (Da):19,309
Last modified:December 12, 2006 - v2
Checksum:i3C005CCD72742A09
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti14S → P in AAO19647 (PubMed:12480930).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY157988 mRNA. Translation: AAO19647.1.
AL049655 Genomic DNA. Translation: CAB41094.1.
CP002686 Genomic DNA. Translation: AEE79309.1.
AF385708 mRNA. Translation: AAK60300.1.
AY078020 mRNA. Translation: AAL77721.1.
PIRiT06730.
RefSeqiNP_191050.1. NM_115347.4.
UniGeneiAt.23079.

Genome annotation databases

EnsemblPlantsiAT3G54900.1; AT3G54900.1; AT3G54900.
GeneIDi824655.
GrameneiAT3G54900.1; AT3G54900.1; AT3G54900.
KEGGiath:AT3G54900.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY157988 mRNA. Translation: AAO19647.1.
AL049655 Genomic DNA. Translation: CAB41094.1.
CP002686 Genomic DNA. Translation: AEE79309.1.
AF385708 mRNA. Translation: AAK60300.1.
AY078020 mRNA. Translation: AAL77721.1.
PIRiT06730.
RefSeqiNP_191050.1. NM_115347.4.
UniGeneiAt.23079.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2MMANMR-A65-173[»]
3IPZX-ray2.40A65-173[»]
ProteinModelPortaliQ84Y95.
SMRiQ84Y95.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi9971. 10 interactors.
IntActiQ84Y95. 4 interactors.
STRINGi3702.AT3G54900.1.

Proteomic databases

PaxDbiQ84Y95.
PRIDEiQ84Y95.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G54900.1; AT3G54900.1; AT3G54900.
GeneIDi824655.
GrameneiAT3G54900.1; AT3G54900.1; AT3G54900.
KEGGiath:AT3G54900.

Organism-specific databases

TAIRiAT3G54900.

Phylogenomic databases

eggNOGiKOG0911. Eukaryota.
COG0278. LUCA.
HOGENOMiHOG000095211.
InParanoidiQ84Y95.
OMAiEVEKAMC.
OrthoDBiEOG09360Q6L.
PhylomeDBiQ84Y95.

Miscellaneous databases

EvolutionaryTraceiQ84Y95.
PROiQ84Y95.

Gene expression databases

GenevisibleiQ84Y95. AT.

Family and domain databases

CDDicd03028. GRX_PICOT_like. 1 hit.
Gene3Di3.40.30.10. 1 hit.
InterProiIPR002109. Glutaredoxin.
IPR033658. GRX_PICOT-like.
IPR004480. Monothiol_GRX-rel.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PANTHERiPTHR10293. PTHR10293. 1 hit.
PfamiPF00462. Glutaredoxin. 1 hit.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 1 hit.
TIGRFAMsiTIGR00365. TIGR00365. 1 hit.
PROSITEiPS51354. GLUTAREDOXIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGRS14_ARATH
AccessioniPrimary (citable) accession number: Q84Y95
Secondary accession number(s): Q9SV38
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: December 12, 2006
Last modified: November 30, 2016
This is version 96 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

The GRXS14-BOLA1 apo-heterodimer model derived from NMR data shows a domain arrangement totally different from the holo-heterodimer showing evidence for a Rieske-type ligation of a [2Fe-2S] cluster.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.