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Protein

Inositol-tetrakisphosphate 1-kinase 1

Gene

ITPK1

Organism
Zea mays (Maize)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Kinase that can phosphorylate various inositol polyphosphate such as Ins(3,4,5,6)P4 or Ins(1,3,4)P3 and participates in phytic acid biosynthesis in developing seeds. Phosphorylates Ins(3,4,5,6)P4 at position 1 to form Ins(1,3,4,5,6)P5. This reaction is thought to have regulatory importance, since Ins(3,4,5,6)P4 is an inhibitor of plasma membrane Ca2+-activated Cl- channels, while Ins(1,3,4,5,6)P5 is not. Also phosphorylates Ins(1,3,4)P3 on O-5 and O-6 to form Ins(1,3,4,6)P4, an essential molecule in the hexakisphosphate (InsP6) pathway. Also able to phosphorylate Ins(3,5,6)P3 but not Ins(1,4,5)P3, Ins(2,4,5)P3, Ins(1,3,4,6)P4 nor Ins(1,3,5,6)P4. Has higher specific activity on Ins(3,4,5,6)P4 than Ins(1,3,4)P3 and Ins(3,5,6)P3. Can also could use Ins(1,2,5,6)P4 as a substrate.1 Publication

Catalytic activityi

ATP + 1D-myo-inositol 3,4,5,6-tetrakisphosphate = ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate.
ATP + 1D-myo-inositol 1,3,4-trisphosphate = ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate.
ATP + 1D-myo-inositol 1,3,4-trisphosphate = ADP + 1D-myo-inositol 1,3,4,6-tetrakisphosphate.

Cofactori

Mg2+By similarityNote: Binds 2 magnesium ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei281D-myo-inositol 1,3,4-trisphosphateBy similarity1
Binding sitei701D-myo-inositol 1,3,4-trisphosphateBy similarity1
Binding sitei105ATPBy similarity1
Binding sitei155ATPBy similarity1
Binding sitei1661D-myo-inositol 1,3,4-trisphosphateBy similarity1
Binding sitei1981D-myo-inositol 1,3,4-trisphosphateBy similarity1
Binding sitei213ATPBy similarity1
Metal bindingi282Magnesium 1By similarity1
Metal bindingi297Magnesium 1By similarity1
Metal bindingi297Magnesium 2By similarity1
Metal bindingi299Magnesium 2By similarity1
Binding sitei2991D-myo-inositol 1,3,4-trisphosphateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi187 – 198ATPBy similarityAdd BLAST12

GO - Molecular functioni

  • ATP binding Source: AgBase
  • inositol-1,3,4,5-tetrakisphosphate 3-phosphatase activity Source: AgBase
  • inositol-1,3,4-trisphosphate 5-kinase activity Source: UniProtKB
  • inositol-1,3,4-trisphosphate 6-kinase activity Source: UniProtKB
  • inositol tetrakisphosphate 1-kinase activity Source: UniProtKB
  • inositol tetrakisphosphate 6-kinase activity Source: AgBase
  • magnesium ion binding Source: InterPro

GO - Biological processi

  • inositol trisphosphate metabolic process Source: InterPro
  • myo-inositol hexakisphosphate biosynthetic process Source: UniProtKB
  • seed development Source: AgBase

Keywordsi

Molecular functionKinase, Transferase
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Inositol-tetrakisphosphate 1-kinase 1 (EC:2.7.1.134)
Alternative name(s):
Inositol 1,3,4-trisphosphate 5/6-kinase 1 (EC:2.7.1.159)
Short name:
Inositol-triphosphate 5/6-kinase 1
Short name:
Ins(1,3,4)P(3) 5/6-kinase 1
Low phytic acid protein 2
ZmIpk
Gene namesi
Name:ITPK1
Synonyms:LPA2
OrganismiZea mays (Maize)
Taxonomic identifieri4577 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogonodaeAndropogoneaeTripsacinaeZea
Proteomesi
  • UP000007305 Componenti: Chromosome 1

Organism-specific databases

MaizeGDBi301214.

Subcellular locationi

Pathology & Biotechi

Disruption phenotypei

Plants display reduced phytic acid content in seeds.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002208431 – 342Inositol-tetrakisphosphate 1-kinase 1Add BLAST342

Proteomic databases

PaxDbiQ84Y01.
PRIDEiQ84Y01.

Expressioni

Tissue specificityi

Expressed in the embryo of 15 day after pollination. Expressed in kernels at earlier stages but at very low levels. Expression in the embryo peaks at 15 days after pollination and then declines. No expression is detected from endosperm and vegetative tissues.1 Publication

Gene expression databases

ExpressionAtlasiQ84Y01. baseline.
GenevisibleiQ84Y01. ZM.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi4577.GRMZM2G456626_P01.

Structurei

3D structure databases

ProteinModelPortaliQ84Y01.
SMRiQ84Y01.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini116 – 332ATP-graspAdd BLAST217

Sequence similaritiesi

Belongs to the ITPK1 family.Curated

Phylogenomic databases

eggNOGiENOG410IHA6. Eukaryota.
ENOG4110KIK. LUCA.
HOGENOMiHOG000220790.
KOiK00913.
OMAiMQDERIC.
OrthoDBiEOG09360FFG.

Family and domain databases

InterProiView protein in InterPro
IPR008656. Inositol_tetrakis-P_1-kinase.
PANTHERiPTHR14217. PTHR14217. 1 hit.
PfamiView protein in Pfam
PF05770. Ins134_P3_kin. 1 hit.
PIRSFiPIRSF038186. ITPK. 1 hit.

Sequencei

Sequence statusi: Complete.

Q84Y01-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASDAAAEPS SGVTHPPRYV IGYALAPKKQ QSFIQPSLVA QAASRGMDLV
60 70 80 90 100
PVDASQPLAE QGPFHLLIHK LYGDDWRAQL VAFAARHPAV PIVDPPHAID
110 120 130 140 150
RLHNRISMLQ VVSELDHAAD QDSTFGIPSQ VVVYDAAALA DFGLLAALRF
160 170 180 190 200
PLIAKPLVAD GTAKSHKMSL VYHREGLGKL RPPLVLQEFV NHGGVIFKVY
210 220 230 240 250
VVGGHVTCVK RRSLPDVSPE DDASAQGSVS FSQVSNLPTE RTAEEYYGEK
260 270 280 290 300
SLEDAVVPPA AFINQIAGGL RRALGLQLFN FDMIRDVRAG DRYLVIDINY
310 320 330 340
FPGYAKMPGY ETVLTDFFWE MVHKDGVGNQ QEEKGANHVV VK
Length:342
Mass (Da):37,313
Last modified:June 1, 2003 - v1
Checksum:iF24DEB3FEBEE6F3C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY172635 mRNA. Translation: AAO17299.1.
RefSeqiNP_001105901.1. NM_001112431.1.
UniGeneiZm.13384.

Genome annotation databases

EnsemblPlantsiZm00001d030083_T001; Zm00001d030083_P001; Zm00001d030083.
GeneIDi732818.
GrameneiZm00001d030083_T001; Zm00001d030083_P001; Zm00001d030083.
KEGGizma:732818.

Similar proteinsi

Entry informationi

Entry nameiITPK1_MAIZE
AccessioniPrimary (citable) accession number: Q84Y01
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: June 1, 2003
Last modified: August 30, 2017
This is version 83 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families