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UniProtKB - Q84XU2 (PPP5_ARATH)

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Protein

Serine/threonine-protein phosphatase 5

Gene

PAPP5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Isoform 2 dephosphorylates phosphorylated phytochromes, with a preference toward Pfr forms, and enhances phytochrome-mediated photoresponses, probably by enhancing their stability and their binding affinity for light signal transducers such as NDPK2. Can use para-nitrophenylphosphate (pNPP) as substrate.1 Publication

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity

Enzyme regulationi

Activated by arachidonic acid (AA).

Kineticsi

Experiments have been done in the presence of 100 µM arachidonic acid (AA).
  1. KM=160 mM for pNPP (at pH 7.5 and 30 degrees Celsius)1 Publication
  1. Vmax=22 µmol/min/mg enzyme with pNPP as substrate (at pH 7.5 and 30 degrees Celsius)1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi282Manganese 1By similarity1
Metal bindingi284Manganese 1By similarity1
Metal bindingi311Manganese 1By similarity1
Metal bindingi311Manganese 2By similarity1
Metal bindingi343Manganese 2By similarity1
Active sitei344Proton donorBy similarity1
Metal bindingi392Manganese 2By similarity1
Metal bindingi467Manganese 2By similarity1

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • phosphoprotein phosphatase activity Source: TAIR
  • protein serine/threonine phosphatase activity Source: TAIR
  • tetrapyrrole binding Source: TAIR
Complete GO annotation...

GO - Biological processi

  • chloroplast-nucleus signaling pathway Source: TAIR
  • negative regulation of chlorophyll biosynthetic process Source: TAIR
  • nucleocytoplasmic transport Source: TAIR
  • red or far-red light signaling pathway Source: TAIR
  • response to cadmium ion Source: TAIR
Complete GO annotation...

Keywordsi

Molecular functionHydrolase, Protein phosphatase
LigandManganese, Metal-binding

Enzyme and pathway databases

ReactomeiR-ATH-5675221. Negative regulation of MAPK pathway.
R-ATH-5693565. Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase 5 (EC:3.1.3.16)
Gene namesi
Name:PAPP5
Synonyms:PP5
Ordered Locus Names:At2g42810
ORF Names:F7D19.19
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRilocus:2052345. AT2G42810.

Subcellular locationi

Isoform 1 :
Isoform 2 :

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei163 – 183HelicalSequence analysisAdd BLAST21
Transmembranei185 – 205HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

  • cytoplasm Source: TAIR
  • cytosol Source: TAIR
  • integral component of endoplasmic reticulum membrane Source: TAIR
  • nuclear envelope Source: TAIR
  • nuclear membrane Source: UniProtKB-SubCell
  • nuclear speck Source: UniProtKB-SubCell
  • nucleus Source: TAIR
  • plasmodesma Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003089881 – 538Serine/threonine-protein phosphatase 5Add BLAST538

Proteomic databases

PaxDbiQ84XU2.
PRIDEiQ84XU2.

PTM databases

iPTMnetiQ84XU2.

Expressioni

Gene expression databases

ExpressionAtlasiQ84XU2. baseline and differential.
GenevisibleiQ84XU2. AT.

Interactioni

Subunit structurei

Interacts with PHYA and PHYB, mostly when they are phosphorylated and in Pfr forms.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
AGO1D6RUV92EBI-4445012,EBI-7498167From Nicotiana tabacum.

Protein-protein interaction databases

BioGridi4218. 3 interactors.
IntActiQ84XU2. 2 interactors.
MINTiMINT-8375614.
STRINGi3702.AT2G42810.2.

Structurei

3D structure databases

ProteinModelPortaliQ84XU2.
SMRiQ84XU2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati13 – 46TPR 1Add BLAST34
Repeati48 – 80TPR 2Add BLAST33
Repeati81 – 114TPR 3Add BLAST34

Domaini

TPR repeats are required for the binding with phytochromes.

Sequence similaritiesi

Belongs to the PPP phosphatase family. PP-5 (PP-T) subfamily.Curated

Keywords - Domaini

Repeat, TPR repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0376. Eukaryota.
COG0639. LUCA.
HOGENOMiHOG000172698.
InParanoidiQ84XU2.
KOiK04460.
OMAiAHMSLGK.
OrthoDBiEOG0936077E.
PhylomeDBiQ84XU2.

Family and domain databases

Gene3Di1.25.40.10. 1 hit.
3.60.21.10. 1 hit.
InterProiView protein in InterPro
IPR004843. Calcineurin-like_PHP_ApaH.
IPR029052. Metallo-depent_PP-like.
IPR013235. PPP_dom.
IPR006186. Ser/Thr-sp_prot-phosphatase.
IPR011236. Ser/Thr_PPase_5.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR001440. TPR_1.
IPR019734. TPR_repeat.
PANTHERiPTHR11668:SF326. PTHR11668:SF326. 1 hit.
PfamiView protein in Pfam
PF00149. Metallophos. 1 hit.
PF08321. PPP5. 1 hit.
PF00515. TPR_1. 1 hit.
PRINTSiPR00114. STPHPHTASE.
SMARTiView protein in SMART
SM00156. PP2Ac. 1 hit.
SM00028. TPR. 3 hits.
SUPFAMiSSF48452. SSF48452. 1 hit.
SSF56300. SSF56300. 1 hit.
PROSITEiView protein in PROSITE
PS50005. TPR. 3 hits.
PS50293. TPR_REGION. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q84XU2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
METKNENSDV SRAEEFKSQA NEAFKGHKYS SAIDLYTKAI ELNSNNAVYW 
        60         70         80         90        100
ANRAFAHTKL EEYGSAIQDA SKAIEVDSRY SKGYYRRGAA YLAMGKFKDA 
       110        120        130        140        150
LKDFQQVKRL SPNDPDATRK LKECEKAVMK LKFEEAISVP VSERRSVAES 
       160        170        180        190        200
IDFHTIGNKP RSSSMPTKTA LAAVVAAVMV VAVRGFATTE ILMVLVSVVL 
       210        220        230        240        250
GTFWWGSFSG KVEPQYSGAR IEGEEVTLDF VKTMMEDFKN QKTLHKRYAY 
       260        270        280        290        300
QIVLQTRQIL LALPSLVDIS VPHGKHITVC GDVHGQFYDL LNIFELNGLP 
       310        320        330        340        350
SEENPYLFNG DFVDRGSFSV EIILTLFAFK CMCPSSIYLA RGNHESKSMN 
       360        370        380        390        400
KIYGFEGEVR SKLSEKFVDL FAEVFCYLPL AHVINGKVFV VHGGLFSVDG 
       410        420        430        440        450
VKLSDIRAID RFCEPPEEGL MCELLWSDPQ PLPGRGPSKR GVGLSFGGDV 
       460        470        480        490        500
TKRFLQDNNL DLLVRSHEVK DEGYEVEHDG KLITVFSAPN YCDQMGNKGA 
       510        520        530 
FIRFEAPDMK PNIVTFSAVP HPDVKPMAYA NNFLRMFN
Length:538
Mass (Da):60,283
Last modified:June 1, 2003 - v1
Checksum:i93ECC937F02D3541
GO
Isoform 2 (identifier: Q84XU2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     157-211: GNKPRSSSMPTKTALAAVVAAVMVVAVRGFATTEILMVLVSVVLGTFWWGSFSGK → E

Note: Partial isoform 2 lacking TPR repeats exhibits enhanced activity at pH 7.5 with pNPP as substrate. This partial protein is in addition inhibited by okadaic acid.
Show »
Length:484
Mass (Da):54,702
Checksum:i04780D6007B69EF8
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_029087157 – 211GNKPR…SFSGK → E in isoform 2. 2 PublicationsAdd BLAST55

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY182779 mRNA. Translation: AAO26216.1.
AC006931 Genomic DNA. Translation: AAD21727.2.
CP002685 Genomic DNA. Translation: AEC10171.1.
CP002685 Genomic DNA. Translation: AEC10172.1.
CP002685 Genomic DNA. Translation: ANM61627.1.
CP002685 Genomic DNA. Translation: ANM61628.1.
AF419574 mRNA. Translation: AAL31906.1.
AY080674 mRNA. Translation: AAL86350.1.
BT010180 mRNA. Translation: AAQ22649.1.
AK221789 mRNA. Translation: BAD93924.1.
PIRiE84858.
RefSeqiNP_001031534.1. NM_001036457.3. [Q84XU2-1]
NP_001323832.1. NM_001336995.1. [Q84XU2-2]
NP_001323833.1. NM_001336996.1. [Q84XU2-2]
NP_565985.1. NM_129842.4. [Q84XU2-2]
UniGeneiAt.23737.

Genome annotation databases

EnsemblPlantsiAT2G42810.2; AT2G42810.2; AT2G42810. [Q84XU2-1]
GeneIDi818881.
GrameneiAT2G42810.2; AT2G42810.2; AT2G42810.
KEGGiath:AT2G42810.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY182779 mRNA. Translation: AAO26216.1.
AC006931 Genomic DNA. Translation: AAD21727.2.
CP002685 Genomic DNA. Translation: AEC10171.1.
CP002685 Genomic DNA. Translation: AEC10172.1.
CP002685 Genomic DNA. Translation: ANM61627.1.
CP002685 Genomic DNA. Translation: ANM61628.1.
AF419574 mRNA. Translation: AAL31906.1.
AY080674 mRNA. Translation: AAL86350.1.
BT010180 mRNA. Translation: AAQ22649.1.
AK221789 mRNA. Translation: BAD93924.1.
PIRiE84858.
RefSeqiNP_001031534.1. NM_001036457.3. [Q84XU2-1]
NP_001323832.1. NM_001336995.1. [Q84XU2-2]
NP_001323833.1. NM_001336996.1. [Q84XU2-2]
NP_565985.1. NM_129842.4. [Q84XU2-2]
UniGeneiAt.23737.

3D structure databases

ProteinModelPortaliQ84XU2.
SMRiQ84XU2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4218. 3 interactors.
IntActiQ84XU2. 2 interactors.
MINTiMINT-8375614.
STRINGi3702.AT2G42810.2.

PTM databases

iPTMnetiQ84XU2.

Proteomic databases

PaxDbiQ84XU2.
PRIDEiQ84XU2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G42810.2; AT2G42810.2; AT2G42810. [Q84XU2-1]
GeneIDi818881.
GrameneiAT2G42810.2; AT2G42810.2; AT2G42810.
KEGGiath:AT2G42810.

Organism-specific databases

AraportiAT2G42810.
TAIRilocus:2052345. AT2G42810.

Phylogenomic databases

eggNOGiKOG0376. Eukaryota.
COG0639. LUCA.
HOGENOMiHOG000172698.
InParanoidiQ84XU2.
KOiK04460.
OMAiAHMSLGK.
OrthoDBiEOG0936077E.
PhylomeDBiQ84XU2.

Enzyme and pathway databases

ReactomeiR-ATH-5675221. Negative regulation of MAPK pathway.
R-ATH-5693565. Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks.

Miscellaneous databases

PROiPR:Q84XU2.

Gene expression databases

ExpressionAtlasiQ84XU2. baseline and differential.
GenevisibleiQ84XU2. AT.

Family and domain databases

Gene3Di1.25.40.10. 1 hit.
3.60.21.10. 1 hit.
InterProiView protein in InterPro
IPR004843. Calcineurin-like_PHP_ApaH.
IPR029052. Metallo-depent_PP-like.
IPR013235. PPP_dom.
IPR006186. Ser/Thr-sp_prot-phosphatase.
IPR011236. Ser/Thr_PPase_5.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR001440. TPR_1.
IPR019734. TPR_repeat.
PANTHERiPTHR11668:SF326. PTHR11668:SF326. 1 hit.
PfamiView protein in Pfam
PF00149. Metallophos. 1 hit.
PF08321. PPP5. 1 hit.
PF00515. TPR_1. 1 hit.
PRINTSiPR00114. STPHPHTASE.
SMARTiView protein in SMART
SM00156. PP2Ac. 1 hit.
SM00028. TPR. 3 hits.
SUPFAMiSSF48452. SSF48452. 1 hit.
SSF56300. SSF56300. 1 hit.
PROSITEiView protein in PROSITE
PS50005. TPR. 3 hits.
PS50293. TPR_REGION. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPPP5_ARATH
AccessioniPrimary (citable) accession number: Q84XU2
Secondary accession number(s): Q56X87
, Q8RXU0, Q8W581, Q9SJH5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: June 1, 2003
Last modified: June 7, 2017
This is version 114 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.