UniProtKB - Q84XU2 (PPP5_ARATH)
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Protein
Serine/threonine-protein phosphatase 5
Gene
PAPP5
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Isoform 2 dephosphorylates phosphorylated phytochromes, with a preference toward Pfr forms, and enhances phytochrome-mediated photoresponses, probably by enhancing their stability and their binding affinity for light signal transducers such as NDPK2. Can use para-nitrophenylphosphate (pNPP) as substrate.1 Publication
Catalytic activityi
[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.
Cofactori
Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity
Enzyme regulationi
Activated by arachidonic acid (AA).
Kineticsi
Experiments have been done in the presence of 100 µM arachidonic acid (AA).
- KM=160 mM for pNPP (at pH 7.5 and 30 degrees Celsius)1 Publication
- Vmax=22 µmol/min/mg enzyme with pNPP as substrate (at pH 7.5 and 30 degrees Celsius)1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 282 | Manganese 1By similarity | 1 | |
Metal bindingi | 284 | Manganese 1By similarity | 1 | |
Metal bindingi | 311 | Manganese 1By similarity | 1 | |
Metal bindingi | 311 | Manganese 2By similarity | 1 | |
Metal bindingi | 343 | Manganese 2By similarity | 1 | |
Active sitei | 344 | Proton donorBy similarity | 1 | |
Metal bindingi | 392 | Manganese 2By similarity | 1 | |
Metal bindingi | 467 | Manganese 2By similarity | 1 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- phosphoprotein phosphatase activity Source: TAIR
- protein serine/threonine phosphatase activity Source: TAIR
- tetrapyrrole binding Source: TAIR
GO - Biological processi
- chloroplast-nucleus signaling pathway Source: TAIR
- negative regulation of chlorophyll biosynthetic process Source: TAIR
- nucleocytoplasmic transport Source: TAIR
- red or far-red light signaling pathway Source: TAIR
- response to cadmium ion Source: TAIR
Keywordsi
Molecular function | Hydrolase, Protein phosphatase |
Ligand | Manganese, Metal-binding |
Enzyme and pathway databases
Reactomei | R-ATH-5675221. Negative regulation of MAPK pathway. |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:PAPP5 Synonyms:PP5 Ordered Locus Names:At2g42810 ORF Names:F7D19.19 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT2G42810. |
TAIRi | locus:2052345. AT2G42810. |
Subcellular locationi
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 163 – 183 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 185 – 205 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Cytoplasm, Endoplasmic reticulum, Membrane, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000308988 | 1 – 538 | Serine/threonine-protein phosphatase 5Add BLAST | 538 |
Proteomic databases
PaxDbi | Q84XU2. |
PRIDEi | Q84XU2. |
PTM databases
iPTMneti | Q84XU2. |
Expressioni
Gene expression databases
ExpressionAtlasi | Q84XU2. baseline and differential. |
Genevisiblei | Q84XU2. AT. |
Interactioni
Subunit structurei
Interacts with PHYA and PHYB, mostly when they are phosphorylated and in Pfr forms.1 Publication
Binary interactionsi
With | Entry | #Exp. | IntAct | Notes |
---|---|---|---|---|
AGO1 | D6RUV9 | 2 | EBI-4445012,EBI-7498167 | From Nicotiana tabacum. |
Protein-protein interaction databases
BioGridi | 4218. 3 interactors. |
IntActi | Q84XU2. 2 interactors. |
MINTi | Q84XU2. |
STRINGi | 3702.AT2G42810.2. |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more detailsFeature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Helixi | 12 – 25 | Combined sources | 14 | |
Helixi | 29 – 42 | Combined sources | 14 | |
Helixi | 47 – 59 | Combined sources | 13 | |
Helixi | 63 – 76 | Combined sources | 14 | |
Helixi | 81 – 93 | Combined sources | 13 | |
Helixi | 97 – 110 | Combined sources | 14 | |
Helixi | 118 – 136 | Combined sources | 19 | |
Helixi | 141 – 143 | Combined sources | 3 | |
Helixi | 147 – 149 | Combined sources | 3 | |
Helixi | 153 – 155 | Combined sources | 3 | |
Beta strandi | 223 – 225 | Combined sources | 3 | |
Helixi | 228 – 239 | Combined sources | 12 | |
Helixi | 246 – 261 | Combined sources | 16 | |
Beta strandi | 265 – 269 | Combined sources | 5 | |
Beta strandi | 276 – 280 | Combined sources | 5 | |
Helixi | 287 – 297 | Combined sources | 11 | |
Beta strandi | 306 – 310 | Combined sources | 5 | |
Beta strandi | 313 – 317 | Combined sources | 5 | |
Helixi | 319 – 332 | Combined sources | 14 | |
Helixi | 334 – 336 | Combined sources | 3 | |
Beta strandi | 337 – 340 | Combined sources | 4 | |
Helixi | 347 – 353 | Combined sources | 7 | |
Helixi | 355 – 362 | Combined sources | 8 | |
Helixi | 365 – 375 | Combined sources | 11 | |
Beta strandi | 380 – 384 | Combined sources | 5 | |
Turni | 385 – 387 | Combined sources | 3 | |
Beta strandi | 388 – 393 | Combined sources | 6 | |
Beta strandi | 397 – 399 | Combined sources | 3 | |
Helixi | 403 – 407 | Combined sources | 5 | |
Beta strandi | 417 – 419 | Combined sources | 3 | |
Helixi | 420 – 426 | Combined sources | 7 | |
Beta strandi | 431 – 437 | Combined sources | 7 | |
Beta strandi | 441 – 446 | Combined sources | 6 | |
Helixi | 448 – 457 | Combined sources | 10 | |
Beta strandi | 461 – 465 | Combined sources | 5 | |
Beta strandi | 473 – 477 | Combined sources | 5 | |
Turni | 478 – 481 | Combined sources | 4 | |
Beta strandi | 482 – 485 | Combined sources | 4 | |
Helixi | 491 – 493 | Combined sources | 3 | |
Beta strandi | 499 – 505 | Combined sources | 7 | |
Turni | 506 – 508 | Combined sources | 3 | |
Beta strandi | 511 – 516 | Combined sources | 6 | |
Turni | 526 – 529 | Combined sources | 4 | |
Helixi | 532 – 535 | Combined sources | 4 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5JJT | X-ray | 2.10 | A/B | 5-537 | [»] | |
ProteinModelPortali | Q84XU2. | |||||
SMRi | Q84XU2. | |||||
ModBasei | Search... | |||||
MobiDBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 13 – 46 | TPR 1Add BLAST | 34 | |
Repeati | 48 – 80 | TPR 2Add BLAST | 33 | |
Repeati | 81 – 114 | TPR 3Add BLAST | 34 |
Domaini
TPR repeats are required for the binding with phytochromes.
Sequence similaritiesi
Keywords - Domaini
Repeat, TPR repeat, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0376. Eukaryota. COG0639. LUCA. |
HOGENOMi | HOG000172698. |
InParanoidi | Q84XU2. |
KOi | K04460. |
OMAi | LYPNHFF. |
OrthoDBi | EOG0936077E. |
PhylomeDBi | Q84XU2. |
Family and domain databases
Gene3Di | 1.25.40.10. 1 hit. 3.60.21.10. 1 hit. |
InterProi | View protein in InterPro IPR004843. Calcineurin-like_PHP_ApaH. IPR029052. Metallo-depent_PP-like. IPR013235. PPP_dom. IPR006186. Ser/Thr-sp_prot-phosphatase. IPR011236. Ser/Thr_PPase_5. IPR013026. TPR-contain_dom. IPR011990. TPR-like_helical_dom_sf. IPR001440. TPR_1. IPR019734. TPR_repeat. |
PANTHERi | PTHR11668:SF391. PTHR11668:SF391. 2 hits. |
Pfami | View protein in Pfam PF00149. Metallophos. 1 hit. PF08321. PPP5. 1 hit. PF00515. TPR_1. 1 hit. |
PRINTSi | PR00114. STPHPHTASE. |
SMARTi | View protein in SMART SM00156. PP2Ac. 1 hit. SM00028. TPR. 3 hits. |
SUPFAMi | SSF48452. SSF48452. 1 hit. |
PROSITEi | View protein in PROSITE PS50005. TPR. 3 hits. PS50293. TPR_REGION. 1 hit. |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basket
Isoform 1 (identifier: Q84XU2-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
10 20 30 40 50
METKNENSDV SRAEEFKSQA NEAFKGHKYS SAIDLYTKAI ELNSNNAVYW
60 70 80 90 100
ANRAFAHTKL EEYGSAIQDA SKAIEVDSRY SKGYYRRGAA YLAMGKFKDA
110 120 130 140 150
LKDFQQVKRL SPNDPDATRK LKECEKAVMK LKFEEAISVP VSERRSVAES
160 170 180 190 200
IDFHTIGNKP RSSSMPTKTA LAAVVAAVMV VAVRGFATTE ILMVLVSVVL
210 220 230 240 250
GTFWWGSFSG KVEPQYSGAR IEGEEVTLDF VKTMMEDFKN QKTLHKRYAY
260 270 280 290 300
QIVLQTRQIL LALPSLVDIS VPHGKHITVC GDVHGQFYDL LNIFELNGLP
310 320 330 340 350
SEENPYLFNG DFVDRGSFSV EIILTLFAFK CMCPSSIYLA RGNHESKSMN
360 370 380 390 400
KIYGFEGEVR SKLSEKFVDL FAEVFCYLPL AHVINGKVFV VHGGLFSVDG
410 420 430 440 450
VKLSDIRAID RFCEPPEEGL MCELLWSDPQ PLPGRGPSKR GVGLSFGGDV
460 470 480 490 500
TKRFLQDNNL DLLVRSHEVK DEGYEVEHDG KLITVFSAPN YCDQMGNKGA
510 520 530
FIRFEAPDMK PNIVTFSAVP HPDVKPMAYA NNFLRMFN
Isoform 2 (identifier: Q84XU2-2) [UniParc]FASTAAdd to basket
The sequence of this isoform differs from the canonical sequence as follows:
157-211: GNKPRSSSMPTKTALAAVVAAVMVVAVRGFATTEILMVLVSVVLGTFWWGSFSGK → E
Note: Partial isoform 2 lacking TPR repeats exhibits enhanced activity at pH 7.5 with pNPP as substrate. This partial protein is in addition inhibited by okadaic acid.
Show »Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_029087 | 157 – 211 | GNKPR…SFSGK → E in isoform 2. 2 PublicationsAdd BLAST | 55 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY182779 mRNA. Translation: AAO26216.1. AC006931 Genomic DNA. Translation: AAD21727.2. CP002685 Genomic DNA. Translation: AEC10171.1. CP002685 Genomic DNA. Translation: AEC10172.1. CP002685 Genomic DNA. Translation: ANM61627.1. CP002685 Genomic DNA. Translation: ANM61628.1. AF419574 mRNA. Translation: AAL31906.1. AY080674 mRNA. Translation: AAL86350.1. BT010180 mRNA. Translation: AAQ22649.1. AK221789 mRNA. Translation: BAD93924.1. |
PIRi | E84858. |
RefSeqi | NP_001031534.1. NM_001036457.3. [Q84XU2-1] NP_001323832.1. NM_001336995.1. [Q84XU2-2] NP_001323833.1. NM_001336996.1. [Q84XU2-2] NP_565985.1. NM_129842.4. [Q84XU2-2] |
UniGenei | At.23737. |
Genome annotation databases
EnsemblPlantsi | AT2G42810.1; AT2G42810.1; AT2G42810. [Q84XU2-2] AT2G42810.2; AT2G42810.2; AT2G42810. [Q84XU2-1] AT2G42810.4; AT2G42810.4; AT2G42810. [Q84XU2-2] AT2G42810.5; AT2G42810.5; AT2G42810. [Q84XU2-2] |
GeneIDi | 818881. |
Gramenei | AT2G42810.1; AT2G42810.1; AT2G42810. [Q84XU2-2] AT2G42810.2; AT2G42810.2; AT2G42810. [Q84XU2-1] AT2G42810.4; AT2G42810.4; AT2G42810. [Q84XU2-2] AT2G42810.5; AT2G42810.5; AT2G42810. [Q84XU2-2] |
KEGGi | ath:AT2G42810. |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Entry informationi
Entry namei | PPP5_ARATH | |
Accessioni | Q84XU2Primary (citable) accession number: Q84XU2 Secondary accession number(s): Q56X87 Q9SJH5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 13, 2007 |
Last sequence update: | June 1, 2003 | |
Last modified: | March 28, 2018 | |
This is version 122 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |