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Protein

Inosine-5'-monophosphate dehydrogenase

Gene

impdh

Organism
Vigna unguiculata (Cowpea)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth.UniRule annotation1 Publication

Catalytic activityi

Inosine 5'-phosphate + NAD+ + H2O = xanthosine 5'-phosphate + NADH.UniRule annotation

Cofactori

K+UniRule annotation

Enzyme regulationi

Mycophenolic acid (MPA) is a non-competitive inhibitor that prevents formation of the closed enzyme conformation by binding to the same site as the amobile flap. In contrast, mizoribine monophosphate (MZP) is a competitive inhibitor that induces the closed conformation. MPA is a potent inhibitor of mammalian IMPDHs but a poor inhibitor of the bacterial enzymes. MZP is a more potent inhibitor of bacterial IMPDH.UniRule annotation

Kineticsi

  1. KM=9.1 µM for Inosine 5'-phosphate1 Publication
  2. KM=18 µM for NAD+1 Publication
  3. KM=1.6 mM for K+1 Publication

    Pathwayi: XMP biosynthesis via de novo pathway

    This protein is involved in step 1 of the subpathway that synthesizes XMP from IMP.UniRule annotation
    Proteins known to be involved in this subpathway in this organism are:
    1. Inosine-5'-monophosphate dehydrogenase (impdh)
    This subpathway is part of the pathway XMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes XMP from IMP, the pathway XMP biosynthesis via de novo pathway and in Purine metabolism.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Metal bindingi316Potassium; via carbonyl oxygenUniRule annotation1
    Metal bindingi318Potassium; via carbonyl oxygenUniRule annotation1
    Binding sitei319IMPUniRule annotation1
    Active sitei321Thioimidate intermediateUniRule annotation1
    Metal bindingi321Potassium; via carbonyl oxygenUniRule annotation1
    Active sitei417Proton acceptorUniRule annotation1
    Binding sitei429IMPUniRule annotation1
    Metal bindingi488Potassium; via carbonyl oxygen; shared with tetrameric partnerUniRule annotation1
    Metal bindingi489Potassium; via carbonyl oxygen; shared with tetrameric partnerUniRule annotation1
    Metal bindingi490Potassium; via carbonyl oxygen; shared with tetrameric partnerUniRule annotation1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi264 – 266NADUniRule annotation3
    Nucleotide bindingi314 – 316NADUniRule annotation3

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionOxidoreductase
    Biological processGMP biosynthesis, Purine biosynthesis
    LigandMetal-binding, NAD, Potassium

    Enzyme and pathway databases

    UniPathwayiUPA00601; UER00295

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Inosine-5'-monophosphate dehydrogenaseUniRule annotation (EC:1.1.1.205UniRule annotation)
    Short name:
    IMP dehydrogenaseUniRule annotation
    Short name:
    IMPDUniRule annotation
    Short name:
    IMPDHUniRule annotation
    Gene namesi
    Name:impdh
    OrganismiVigna unguiculata (Cowpea)
    Taxonomic identifieri3917 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaePhaseoleaeVigna

    Subcellular locationi

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00004156811 – 502Inosine-5'-monophosphate dehydrogenaseAdd BLAST502

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotation1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliQ84XA3
    SMRiQ84XA3
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini168 – 224CBSUniRule annotationAdd BLAST57

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni354 – 356IMP bindingUniRule annotation3
    Regioni377 – 378IMP bindingUniRule annotation2
    Regioni401 – 405IMP bindingUniRule annotation5

    Sequence similaritiesi

    Belongs to the IMPDH/GMPR family.UniRule annotation

    Keywords - Domaini

    CBS domain

    Family and domain databases

    CDDicd00381 IMPDH, 1 hit
    Gene3Di3.20.20.70, 2 hits
    HAMAPiMF_01964 IMPDH, 1 hit
    InterProiView protein in InterPro
    IPR013785 Aldolase_TIM
    IPR005990 IMP_DH
    IPR015875 IMP_DH/GMP_Rdtase_CS
    IPR001093 IMP_DH_GMPRt
    PfamiView protein in Pfam
    PF00478 IMPDH, 1 hit
    PIRSFiPIRSF000130 IMPDH, 1 hit
    TIGRFAMsiTIGR01302 IMP_dehydrog, 1 hit
    PROSITEiView protein in PROSITE
    PS00487 IMP_DH_GMP_RED, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Q84XA3-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MDFTPPPIED GFTAEKLFSQ GFSYTYDDVI FLPHYIDFAA DAVDLSTRLT
    60 70 80 90 100
    RRLPLAVPLV ASPMDTVSES AMASAMASLG GIAIVHSNVP AAAQASLVRA
    110 120 130 140 150
    AKSRRVPILS EPAFAAPSAV IEHEDDFAAS PFLLVTDIGA AGGKLLGYVA
    160 170 180 190 200
    KRDWTNQKDK SLRVGDYMAP PPRRAPWNAD LNKIHEIMEN EKSGAVALER
    210 220 230 240 250
    DGEVVDLVVR EEVERVKGYP KLAAPATVGP DGEFMVGAAM GTREDDKERL
    260 270 280 290 300
    KHLVKAGVNV VVLDSSQGNS IYQLEMVKYV KSVYPELDVI GGNVVTMYQA
    310 320 330 340 350
    ENLIQAGVDG LRVGMGSGSI CTTQEVCAVG RGQATAVYKV SSIAYKSGVP
    360 370 380 390 400
    VIADGGISNS GHIVKALSLG ASTAMMGSFL AGSHEAPGAY VYQNGQRVKK
    410 420 430 440 450
    YRGMGSLEAM TKGSDARYLG DTAKLKIAQG VVGAVKDKGS VLNFIPYTLQ
    460 470 480 490 500
    AVRQGFQDIG ASSLQSAHDL LRSRVLRLEV RTGAAQVEGG VHGLVSYEKK

    YY
    Length:502
    Mass (Da):53,450
    Last modified:June 1, 2003 - v1
    Checksum:i61773EBE986FFD30
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AY193837 mRNA Translation: AAO40253.1

    Similar proteinsi

    Entry informationi

    Entry nameiIMDH_VIGUN
    AccessioniPrimary (citable) accession number: Q84XA3
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 22, 2012
    Last sequence update: June 1, 2003
    Last modified: May 23, 2018
    This is version 79 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

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