Reviewed, UniProtKB/Swiss-Prot Q84WW2 (6PGL5_ARATH)

Names and origin

Protein names

Recommended name:
    Probable 6-phosphogluconolactonase 5, chloroplastic
      Short name=6PGL 5
    EC=3.1.1.31
Alternative name(s):
    Protein EMBRYO DEFECTIVE 2024

Gene names

Name:	EMB2024
Ordered Locus Names:	At5g24400
ORF Names:	K16H17.11

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	325 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Hydrolysis of 6-phosphogluconolactone to 6-phosphogluconate By similarity.
Catalytic activity	6-phospho-D-glucono-1,5-lactone + H2O = 6-phospho-D-gluconate.
Pathway	Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 2/3.
Subcellular location	Plastid › chloroplast Potential.
Sequence similarities	Belongs to the glucosamine/galactosamine-6-phosphate isomerase family. 6-phosphogluconolactonase subfamily.

Ontologies

Keywords
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pentose-phosphate shunt Inferred from electronic annotation. Source: InterPro
Cellular component	chloroplast stroma Inferred from direct assay. Source: TAIR peroxisome Inferred from direct assay. Source: TAIR
Molecular function	6-phosphogluconolactonase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 68	68	Chloroplast Potential
	Chain	69 – 325	257	Probable 6-phosphogluconolactonase 5, chloroplastic		PRO_0000288672
Experimental info
	Sequence conflict	30	1	P → L in AAM63366. Ref.3
	Sequence conflict	33	1	Y → H in AAM63366. Ref.3
	Sequence conflict	93 – 94	2	VI → AV in AAM63366. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q84WW2-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 0FBC6E95F9C073DC	FASTA	325	35,645
	10 20 30 40 50 60 MASSSCFLRS ILFSSPTNLR SNHHLPTFFP KNYLICSHST SSRFESLSVS SIGTGSTKKS 70 80 90 100 110 120 SDTRRKVKSM ATTNIGKEEK KRVEIYDLEE NLVIDLAKFT ADLSDKFCKE RGAFTVVVSG 130 140 150 160 170 180 GSLIKSLRKL VESPYVDSID WARWHFFWVD ERVVPKNHDD SNYKLAYDSF LSKVPIPPGN 190 200 210 220 230 240 VYAINEALSA EAAADDYETC LKHLVNTNIL RVSESTGFPK FDLMLLGMGP DGHVASLFPG 250 260 270 280 290 300 HGLCNESKKW VVSISDSPKP PSERITFTFP VINSSAHVAL VVCGSGKAEA VEAALKKTGN 310 320 VPPAGSVSAE DELVWFLDKP ASSKL

References

[1]	"Structural analysis of Arabidopsis thaliana chromosome 5. VIII. Sequence features of the regions of 1,081,958 bp covered by seventeen physically assigned P1 and TAC clones." Asamizu E., Sato S., Kaneko T., Nakamura Y., Kotani H., Miyajima N., Tabata S. DNA Res. 5:379-391(1998) [PubMed: 10048488] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[3]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"The Arabidopsis thaliana transcribed genome: the GDR cDNA program." Desprez T., Amselem J., Chiapello H., Caboche M., Hoefte H. Submitted (SEP-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 242-325. Strain: cv. Columbia. Tissue: Seedling.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB016884 Genomic DNA. Translation: BAB11233.1. Different initiation. BT001923 mRNA. Translation: AAN71922.1. AY086161 mRNA. Translation: AAM63366.1. Z26557 mRNA. Translation: CAA81328.1.
IPI	IPI00547519.
RefSeq	NP_568445.1.
UniGene	At.19070
3D structure databases
HSSP	HSSP built from PDB template 1VL1 based on UniProtKB Q9X0N8.
SMR	Q84WW2. Positions 82-319.
ModBase	Search...
Protein-protein interaction databases
STRING	Q84WW2.
Proteomic databases
PRIDE	Q84WW2.
Genome annotation databases
GeneID	832511.
GenomeReviews	Gene locus AT5G24400 in contig BA000015_GR.
KEGG	ath:AT5G24400.
NMPDR	fig|3702.1.peg.24632.
Organism-specific databases
TAIR	At5g24400.
Phylogenomic databases
eggNOG	KOG3147.
HOGENOM	HBG725991.
InParanoid	Q84WW2.
OMA	PSERITF.
PhylomeDB	Q84WW2.
Enzyme and pathway databases
BRENDA	3.1.1.31. 302.
Gene expression databases
ArrayExpress	Q84WW2.
Genevestigator	Q84WW2.
Family and domain databases
InterPro	IPR005900. 6-phosphogluconolactonase. IPR006148. Glc/Gal-6P_isomerase. [Graphical view]
PANTHER	PTHR11054. Phosphogluconlac. 1 hit.
Pfam	PF01182. Glucosamine_iso. 1 hit. [Graphical view]
TIGRFAMs	TIGR01198. pgl. 1 hit.
ProtoNet	Search...

Entry information

Entry name

6PGL5_ARATH

Accession

Primary (citable) accession number: Q84WW2
Secondary accession number(s): Q42135, Q8LD77, Q9FIN2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	June 1, 2003
Last modified:	February 9, 2010
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)