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Protein

Probable 6-phosphogluconolactonase 5, chloroplastic

Gene

EMB2024

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Hydrolysis of 6-phosphogluconolactone to 6-phosphogluconate.By similarity

Catalytic activityi

6-phospho-D-glucono-1,5-lactone + H2O = 6-phospho-D-gluconate.

Pathway: pentose phosphate pathway

This protein is involved in step 2 of the subpathway that synthesizes D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage).
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Glucose-6-phosphate 1-dehydrogenase 3, chloroplastic (At1g24280), Glucose-6-phosphate 1-dehydrogenase 1, chloroplastic (APG1), Glucose-6-phosphate 1-dehydrogenase (At3g27300), Glucose-6-phosphate 1-dehydrogenase, cytoplasmic isoform 2 (ACG12), Glucose-6-phosphate 1-dehydrogenase (G6PD4), Glucose-6-phosphate 1-dehydrogenase (At1g09420), Glucose-6-phosphate 1-dehydrogenase 2, chloroplastic (At5g13110), Glucose-6-phosphate 1-dehydrogenase (At5g35790), Glucose-6-phosphate 1-dehydrogenase, cytoplasmic isoform 1 (ACG9), Glucose-6-phosphate 1-dehydrogenase (At5g40760), Glucose-6-phosphate 1-dehydrogenase 4, chloroplastic (At1g09420)
  2. Probable 6-phosphogluconolactonase 5, chloroplastic (EMB2024), Probable 6-phosphogluconolactonase 2 (At3g49360), Probable 6-phosphogluconolactonase 3 (At5g24420), Probable 6-phosphogluconolactonase 4 (At5g24410), Probable 6-phosphogluconolactonase 1 (At1g13700)
  3. 6-phosphogluconate dehydrogenase, decarboxylating 1, chloroplastic (At1g64190), 6-phosphogluconate dehydrogenase, decarboxylating 2, chloroplastic (At5g41670), 6-phosphogluconate dehydrogenase, decarboxylating 3 (At3g02360)
This subpathway is part of the pathway pentose phosphate pathway, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage), the pathway pentose phosphate pathway and in Carbohydrate degradation.

GO - Molecular functioni

  • 6-phosphogluconolactonase activity Source: TAIR

GO - Biological processi

  • carbohydrate metabolic process Source: InterPro
  • cellular response to redox state Source: TAIR
  • defense response to bacterium Source: TAIR
  • defense response to oomycetes Source: TAIR
  • nitrate assimilation Source: TAIR
  • pentose-phosphate shunt Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BioCyciARA:AT5G24400-MONOMER.
BRENDAi3.1.1.31. 399.
ReactomeiREACT_281719. Pentose phosphate pathway (hexose monophosphate shunt).
UniPathwayiUPA00115; UER00409.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable 6-phosphogluconolactonase 5, chloroplastic (EC:3.1.1.31)
Short name:
6PGL 5
Alternative name(s):
Protein EMBRYO DEFECTIVE 2024
Gene namesi
Name:EMB2024
Ordered Locus Names:At5g24400
ORF Names:K16H17.11
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G24400.

Subcellular locationi

GO - Cellular componenti

  • chloroplast Source: TAIR
  • chloroplast stroma Source: TAIR
  • peroxisome Source: TAIR
  • plastid Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 6868ChloroplastSequence AnalysisAdd
BLAST
Chaini69 – 325257Probable 6-phosphogluconolactonase 5, chloroplasticPRO_0000288672Add
BLAST

Proteomic databases

PaxDbiQ84WW2.
PRIDEiQ84WW2.

Interactioni

Protein-protein interaction databases

BioGridi17786. 1 interaction.
STRINGi3702.AT5G24400.1.

Structurei

3D structure databases

ProteinModelPortaliQ84WW2.
SMRiQ84WW2. Positions 83-325.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0363.
HOGENOMiHOG000256285.
InParanoidiQ84WW2.
KOiK01057.
OMAiCKERGAF.
PhylomeDBiQ84WW2.

Family and domain databases

InterProiIPR005900. 6-phosphogluconolactonase_DevB.
IPR006148. Glc/Gal-6P_isomerase.
[Graphical view]
PfamiPF01182. Glucosamine_iso. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01198. pgl. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q84WW2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASSSCFLRS ILFSSPTNLR SNHHLPTFFP KNYLICSHST SSRFESLSVS
60 70 80 90 100
SIGTGSTKKS SDTRRKVKSM ATTNIGKEEK KRVEIYDLEE NLVIDLAKFT
110 120 130 140 150
ADLSDKFCKE RGAFTVVVSG GSLIKSLRKL VESPYVDSID WARWHFFWVD
160 170 180 190 200
ERVVPKNHDD SNYKLAYDSF LSKVPIPPGN VYAINEALSA EAAADDYETC
210 220 230 240 250
LKHLVNTNIL RVSESTGFPK FDLMLLGMGP DGHVASLFPG HGLCNESKKW
260 270 280 290 300
VVSISDSPKP PSERITFTFP VINSSAHVAL VVCGSGKAEA VEAALKKTGN
310 320
VPPAGSVSAE DELVWFLDKP ASSKL
Length:325
Mass (Da):35,645
Last modified:June 1, 2003 - v1
Checksum:i0FBC6E95F9C073DC
GO

Sequence cautioni

The sequence BAB11233.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti30 – 301P → L in AAM63366 (Ref. 4) Curated
Sequence conflicti33 – 331Y → H in AAM63366 (Ref. 4) Curated
Sequence conflicti93 – 942VI → AV in AAM63366 (Ref. 4) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB016884 Genomic DNA. Translation: BAB11233.1. Different initiation.
CP002688 Genomic DNA. Translation: AED93307.1.
BT001923 mRNA. Translation: AAN71922.1.
AY086161 mRNA. Translation: AAM63366.1.
Z26557 mRNA. Translation: CAA81328.1.
RefSeqiNP_568445.1. NM_122348.2.
UniGeneiAt.19070.

Genome annotation databases

EnsemblPlantsiAT5G24400.1; AT5G24400.1; AT5G24400.
GeneIDi832511.
KEGGiath:AT5G24400.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB016884 Genomic DNA. Translation: BAB11233.1. Different initiation.
CP002688 Genomic DNA. Translation: AED93307.1.
BT001923 mRNA. Translation: AAN71922.1.
AY086161 mRNA. Translation: AAM63366.1.
Z26557 mRNA. Translation: CAA81328.1.
RefSeqiNP_568445.1. NM_122348.2.
UniGeneiAt.19070.

3D structure databases

ProteinModelPortaliQ84WW2.
SMRiQ84WW2. Positions 83-325.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi17786. 1 interaction.
STRINGi3702.AT5G24400.1.

Proteomic databases

PaxDbiQ84WW2.
PRIDEiQ84WW2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G24400.1; AT5G24400.1; AT5G24400.
GeneIDi832511.
KEGGiath:AT5G24400.

Organism-specific databases

TAIRiAT5G24400.

Phylogenomic databases

eggNOGiCOG0363.
HOGENOMiHOG000256285.
InParanoidiQ84WW2.
KOiK01057.
OMAiCKERGAF.
PhylomeDBiQ84WW2.

Enzyme and pathway databases

UniPathwayiUPA00115; UER00409.
BioCyciARA:AT5G24400-MONOMER.
BRENDAi3.1.1.31. 399.
ReactomeiREACT_281719. Pentose phosphate pathway (hexose monophosphate shunt).

Miscellaneous databases

PROiQ84WW2.

Family and domain databases

InterProiIPR005900. 6-phosphogluconolactonase_DevB.
IPR006148. Glc/Gal-6P_isomerase.
[Graphical view]
PfamiPF01182. Glucosamine_iso. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01198. pgl. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structural analysis of Arabidopsis thaliana chromosome 5. VIII. Sequence features of the regions of 1,081,958 bp covered by seventeen physically assigned P1 and TAC clones."
    Asamizu E., Sato S., Kaneko T., Nakamura Y., Kotani H., Miyajima N., Tabata S.
    DNA Res. 5:379-391(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. "The Arabidopsis thaliana transcribed genome: the GDR cDNA program."
    Desprez T., Amselem J., Chiapello H., Caboche M., Hoefte H.
    Submitted (SEP-1993) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 242-325.
    Strain: cv. Columbia.
    Tissue: Seedling.

Entry informationi

Entry namei6PGL5_ARATH
AccessioniPrimary (citable) accession number: Q84WW2
Secondary accession number(s): Q42135, Q8LD77, Q9FIN2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: June 1, 2003
Last modified: June 24, 2015
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.