Q84WV2 (BGL20_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-glucosidase 20
Short name=AtBGLU20
EC=3.2.1.21

Gene names

Name:	BGLU20
Ordered Locus Names:	At1g75940
ORF Names:	T4O12.15

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	535 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.
Subcellular location	Endoplasmic reticulum lumen Potential.
Sequence similarities	Belongs to the glycosyl hydrolase 1 family.
Sequence caution	The sequence AAF26759.2 differs from that shown. Reason: Erroneous gene model prediction. The predicted gene At1g75930 has been split into 2 genes: At1g75930 and At1g75940.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	endoplasmic reticulum lumen Inferred from sequence or structural similarity Ref.1. Source: TAIR membrane Inferred from direct assay PubMed 17432890. Source: TAIR
Molecular_function	beta-glucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Chain	25 – 535	511	Beta-glucosidase 20		PRO_0000389582
Regions
	Region	482 – 483	2	Substrate binding By similarity
	Motif	532 – 535	4	Prevents secretion from ER Potential
Sites
	Active site	205	1	Proton donor By similarity
	Active site	424	1	Nucleophile By similarity
	Binding site	56	1	Substrate By similarity
	Binding site	159	1	Substrate By similarity
	Binding site	204	1	Substrate By similarity
	Binding site	351	1	Substrate By similarity
	Binding site	475	1	Substrate By similarity
Amino acid modifications
	Glycosylation	187	1	N-linked (GlcNAc...) Potential
	Glycosylation	468	1	N-linked (GlcNAc...) Potential
	Glycosylation	501	1	N-linked (GlcNAc...) Potential
	Disulfide bond	224 ↔ 235		By similarity
Experimental info
	Sequence conflict	362	1	P → T in AAC39504. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q84WV2 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: C717D02DA37F1C81	FASTA	535	61,675
	10 20 30 40 50 60 MGRFHKFPLL GLVLFLGLTG SLIAANEYAC SSTDIHFTRA NFPKGFIFGT ATAAFQVEGA 70 80 90 100 110 120 VNEGCRGPSM WDVYTKKFPH KCNYHNADVA VDFYHRYKED IKLMKNLNTD GFRFSIAWPR 130 140 150 160 170 180 IFPHGRMEKG ISKAGVQYYH DLIDELLANG ITPLVTVFHW DTPQDLEDEY GGFLSDRIIK 190 200 210 220 230 240 DFTEYANFTF QEYGDKVKHW ITFNEPWVFS RAGYDIGNKA PGRCSKYIKE HGEMCHDGRS 250 260 270 280 290 300 GHEAYIVSHN MLLAHADAVD AFRKCDKCKG GKIGIAHSPA WFEAHELSDE EHETPVTGLI 310 320 330 340 350 360 DFILGWHLHP TTYGDYPQSM KDHIGHRLPK FTEAQKEKLK NSADFVGINY YTSVFALHDE 370 380 390 400 410 420 EPDPSQPSWQ SDSLVDWEPR YVDKFNAFAN KPDVAKVEVY AKGLRSLLKY IKDKYGNPEI 430 440 450 460 470 480 MITENGYGED LGEQDTSLVV ALSDQHRTYY IQKHLLSLHE AICDDKVNVT GYFHWSLMDN 490 500 510 520 530 FEWQDGYKAR FGLYYVDYKN NLTRHEKLSA QWYSSFLHDG SKEFEIEHEF EHDEL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification, sequence analysis and expression studies of novel anther-specific genes of Arabidopsis thaliana." Rubinelli P., Hu Y., Ma H. Plant Mol. Biol. 37:607-619(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Functional genomic analysis of Arabidopsis thaliana glycoside hydrolase family 1." Xu Z., Escamilla-Trevino L.L., Zeng L., Lalgondar M., Bevan D.R., Winkel B.S.J., Mohamed A., Cheng C.-L., Shih M.-C., Poulton J.E., Esen A. Plant Mol. Biol. 55:343-367(2004) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF037590 mRNA. Translation: AAC39504.1. AC007396 Genomic DNA. Translation: AAF26759.2. Sequence problems. CP002684 Genomic DNA. Translation: AEE35779.1. AY074517 mRNA. Translation: AAL67131.1. BT002735 mRNA. Translation: AAO22564.1.
IPI	IPI00524528.
PIR	T52048.
RefSeq	NP_177722.1. NM_106244.2.
UniGene	At.10790.
3D structure databases
ProteinModelPortal	Q84WV2.
SMR	Q84WV2. Positions 37-517.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT1G75940.1-P.
Protein family/group databases
CAZy	GH1. Glycoside Hydrolase Family 1.
Proteomic databases
PaxDb	Q84WV2.
PRIDE	Q84WV2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G75940.1; AT1G75940.1; AT1G75940.
GeneID	843927.
KEGG	ath:AT1G75940.
Organism-specific databases
TAIR	At1g75940.
Phylogenomic databases
eggNOG	COG2723.
HOGENOM	HOG000088630.
InParanoid	Q84WV2.
KO	K01188.
OMA	DIANAHA.
PhylomeDB	Q84WV2.
Gene expression databases
Genevestigator	Q84WV2.
Family and domain databases
Gene3D	3.20.20.80. 1 hit.
InterPro	IPR001360. Glyco_hydro_1. IPR018120. Glyco_hydro_1_AS. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
PANTHER	PTHR10353. PTHR10353. 1 hit.
Pfam	PF00232. Glyco_hydro_1. 1 hit. [Graphical view]
PRINTS	PR00131. GLHYDRLASE1.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00014. ER_TARGET. 1 hit. PS00572. GLYCOSYL_HYDROL_F1_1. False negative. PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BGL20_ARATH

Accession

Primary (citable) accession number: Q84WV2
Secondary accession number(s): O49117, Q8VXW3, Q9LQS3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 24, 2009
Last sequence update:	June 1, 2003
Last modified:	May 29, 2013
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program