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Protein

Beta-glucosidase 20

Gene

BGLU20

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei56 – 561SubstrateBy similarity
Binding sitei159 – 1591SubstrateBy similarity
Binding sitei204 – 2041SubstrateBy similarity
Active sitei205 – 2051Proton donorBy similarity
Binding sitei351 – 3511SubstrateBy similarity
Active sitei424 – 4241NucleophileBy similarity
Binding sitei475 – 4751SubstrateBy similarity

GO - Molecular functioni

  1. beta-glucosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BioCyciARA:AT1G75940-MONOMER.

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-glucosidase 20 (EC:3.2.1.21)
Short name:
AtBGLU20
Gene namesi
Name:BGLU20
Ordered Locus Names:At1g75940
ORF Names:T4O12.15
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G75940.

Subcellular locationi

Endoplasmic reticulum lumen PROSITE-ProRule annotation

GO - Cellular componenti

  1. endoplasmic reticulum lumen Source: TAIR
  2. membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Sequence AnalysisAdd
BLAST
Chaini25 – 535511Beta-glucosidase 20PRO_0000389582Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi187 – 1871N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi224 ↔ 235By similarity
Glycosylationi468 – 4681N-linked (GlcNAc...)Sequence Analysis
Glycosylationi501 – 5011N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ84WV2.
PRIDEiQ84WV2.

Expressioni

Gene expression databases

GenevestigatoriQ84WV2.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT1G75940.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ84WV2.
SMRiQ84WV2. Positions 37-517.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni482 – 4832Substrate bindingBy similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi532 – 5354Prevents secretion from ERPROSITE-ProRule annotation

Sequence similaritiesi

Belongs to the glycosyl hydrolase 1 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG2723.
HOGENOMiHOG000088630.
InParanoidiQ84WV2.
KOiK01188.
OMAiHNDISAN.
PhylomeDBiQ84WV2.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00014. ER_TARGET. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q84WV2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGRFHKFPLL GLVLFLGLTG SLIAANEYAC SSTDIHFTRA NFPKGFIFGT
60 70 80 90 100
ATAAFQVEGA VNEGCRGPSM WDVYTKKFPH KCNYHNADVA VDFYHRYKED
110 120 130 140 150
IKLMKNLNTD GFRFSIAWPR IFPHGRMEKG ISKAGVQYYH DLIDELLANG
160 170 180 190 200
ITPLVTVFHW DTPQDLEDEY GGFLSDRIIK DFTEYANFTF QEYGDKVKHW
210 220 230 240 250
ITFNEPWVFS RAGYDIGNKA PGRCSKYIKE HGEMCHDGRS GHEAYIVSHN
260 270 280 290 300
MLLAHADAVD AFRKCDKCKG GKIGIAHSPA WFEAHELSDE EHETPVTGLI
310 320 330 340 350
DFILGWHLHP TTYGDYPQSM KDHIGHRLPK FTEAQKEKLK NSADFVGINY
360 370 380 390 400
YTSVFALHDE EPDPSQPSWQ SDSLVDWEPR YVDKFNAFAN KPDVAKVEVY
410 420 430 440 450
AKGLRSLLKY IKDKYGNPEI MITENGYGED LGEQDTSLVV ALSDQHRTYY
460 470 480 490 500
IQKHLLSLHE AICDDKVNVT GYFHWSLMDN FEWQDGYKAR FGLYYVDYKN
510 520 530
NLTRHEKLSA QWYSSFLHDG SKEFEIEHEF EHDEL
Length:535
Mass (Da):61,675
Last modified:June 1, 2003 - v1
Checksum:iC717D02DA37F1C81
GO

Sequence cautioni

The sequence AAF26759.2 differs from that shown. Reason: Erroneous gene model prediction. The predicted gene At1g75930 has been split into 2 genes: At1g75930 and At1g75940.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti362 – 3621P → T in AAC39504. (PubMed:9687065)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF037590 mRNA. Translation: AAC39504.1.
AC007396 Genomic DNA. Translation: AAF26759.2. Sequence problems.
CP002684 Genomic DNA. Translation: AEE35779.1.
AY074517 mRNA. Translation: AAL67131.1.
BT002735 mRNA. Translation: AAO22564.1.
PIRiT52048.
RefSeqiNP_177722.1. NM_106244.2.
UniGeneiAt.10790.

Genome annotation databases

EnsemblPlantsiAT1G75940.1; AT1G75940.1; AT1G75940.
GeneIDi843927.
KEGGiath:AT1G75940.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF037590 mRNA. Translation: AAC39504.1.
AC007396 Genomic DNA. Translation: AAF26759.2. Sequence problems.
CP002684 Genomic DNA. Translation: AEE35779.1.
AY074517 mRNA. Translation: AAL67131.1.
BT002735 mRNA. Translation: AAO22564.1.
PIRiT52048.
RefSeqiNP_177722.1. NM_106244.2.
UniGeneiAt.10790.

3D structure databases

ProteinModelPortaliQ84WV2.
SMRiQ84WV2. Positions 37-517.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT1G75940.1-P.

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

Proteomic databases

PaxDbiQ84WV2.
PRIDEiQ84WV2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G75940.1; AT1G75940.1; AT1G75940.
GeneIDi843927.
KEGGiath:AT1G75940.

Organism-specific databases

TAIRiAT1G75940.

Phylogenomic databases

eggNOGiCOG2723.
HOGENOMiHOG000088630.
InParanoidiQ84WV2.
KOiK01188.
OMAiHNDISAN.
PhylomeDBiQ84WV2.

Enzyme and pathway databases

BioCyciARA:AT1G75940-MONOMER.

Gene expression databases

GenevestigatoriQ84WV2.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00014. ER_TARGET. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification, sequence analysis and expression studies of novel anther-specific genes of Arabidopsis thaliana."
    Rubinelli P., Hu Y., Ma H.
    Plant Mol. Biol. 37:607-619(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. Cited for: GENE FAMILY, NOMENCLATURE.

Entry informationi

Entry nameiBGL20_ARATH
AccessioniPrimary (citable) accession number: Q84WV2
Secondary accession number(s): O49117, Q8VXW3, Q9LQS3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 24, 2009
Last sequence update: June 1, 2003
Last modified: January 7, 2015
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.