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Q84WV2

- BGL20_ARATH

UniProt

Q84WV2 - BGL20_ARATH

Protein

Beta-glucosidase 20

Gene

BGLU20

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 80 (01 Oct 2014)
      Sequence version 1 (01 Jun 2003)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei56 – 561SubstrateBy similarity
    Binding sitei159 – 1591SubstrateBy similarity
    Binding sitei204 – 2041SubstrateBy similarity
    Active sitei205 – 2051Proton donorBy similarity
    Binding sitei351 – 3511SubstrateBy similarity
    Active sitei424 – 4241NucleophileBy similarity
    Binding sitei475 – 4751SubstrateBy similarity

    GO - Molecular functioni

    1. beta-glucosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Enzyme and pathway databases

    BioCyciARA:AT1G75940-MONOMER.

    Protein family/group databases

    CAZyiGH1. Glycoside Hydrolase Family 1.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-glucosidase 20 (EC:3.2.1.21)
    Short name:
    AtBGLU20
    Gene namesi
    Name:BGLU20
    Ordered Locus Names:At1g75940
    ORF Names:T4O12.15
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G75940.

    Subcellular locationi

    Endoplasmic reticulum lumen PROSITE-ProRule annotation

    GO - Cellular componenti

    1. endoplasmic reticulum lumen Source: TAIR
    2. membrane Source: TAIR

    Keywords - Cellular componenti

    Endoplasmic reticulum

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2424Sequence AnalysisAdd
    BLAST
    Chaini25 – 535511Beta-glucosidase 20PRO_0000389582Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi187 – 1871N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi224 ↔ 235By similarity
    Glycosylationi468 – 4681N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi501 – 5011N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiQ84WV2.
    PRIDEiQ84WV2.

    Expressioni

    Gene expression databases

    GenevestigatoriQ84WV2.

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT1G75940.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ84WV2.
    SMRiQ84WV2. Positions 37-517.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni482 – 4832Substrate bindingBy similarity

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi532 – 5354Prevents secretion from ERPROSITE-ProRule annotation

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 1 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG2723.
    HOGENOMiHOG000088630.
    InParanoidiQ84WV2.
    KOiK01188.
    OMAiHNDISAN.
    PhylomeDBiQ84WV2.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR001360. Glyco_hydro_1.
    IPR018120. Glyco_hydro_1_AS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR10353. PTHR10353. 1 hit.
    PfamiPF00232. Glyco_hydro_1. 1 hit.
    [Graphical view]
    PRINTSiPR00131. GLHYDRLASE1.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    PROSITEiPS00014. ER_TARGET. 1 hit.
    PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q84WV2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGRFHKFPLL GLVLFLGLTG SLIAANEYAC SSTDIHFTRA NFPKGFIFGT    50
    ATAAFQVEGA VNEGCRGPSM WDVYTKKFPH KCNYHNADVA VDFYHRYKED 100
    IKLMKNLNTD GFRFSIAWPR IFPHGRMEKG ISKAGVQYYH DLIDELLANG 150
    ITPLVTVFHW DTPQDLEDEY GGFLSDRIIK DFTEYANFTF QEYGDKVKHW 200
    ITFNEPWVFS RAGYDIGNKA PGRCSKYIKE HGEMCHDGRS GHEAYIVSHN 250
    MLLAHADAVD AFRKCDKCKG GKIGIAHSPA WFEAHELSDE EHETPVTGLI 300
    DFILGWHLHP TTYGDYPQSM KDHIGHRLPK FTEAQKEKLK NSADFVGINY 350
    YTSVFALHDE EPDPSQPSWQ SDSLVDWEPR YVDKFNAFAN KPDVAKVEVY 400
    AKGLRSLLKY IKDKYGNPEI MITENGYGED LGEQDTSLVV ALSDQHRTYY 450
    IQKHLLSLHE AICDDKVNVT GYFHWSLMDN FEWQDGYKAR FGLYYVDYKN 500
    NLTRHEKLSA QWYSSFLHDG SKEFEIEHEF EHDEL 535
    Length:535
    Mass (Da):61,675
    Last modified:June 1, 2003 - v1
    Checksum:iC717D02DA37F1C81
    GO

    Sequence cautioni

    The sequence AAF26759.2 differs from that shown. Reason: Erroneous gene model prediction. The predicted gene At1g75930 has been split into 2 genes: At1g75930 and At1g75940.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti362 – 3621P → T in AAC39504. (PubMed:9687065)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF037590 mRNA. Translation: AAC39504.1.
    AC007396 Genomic DNA. Translation: AAF26759.2. Sequence problems.
    CP002684 Genomic DNA. Translation: AEE35779.1.
    AY074517 mRNA. Translation: AAL67131.1.
    BT002735 mRNA. Translation: AAO22564.1.
    PIRiT52048.
    RefSeqiNP_177722.1. NM_106244.2.
    UniGeneiAt.10790.

    Genome annotation databases

    EnsemblPlantsiAT1G75940.1; AT1G75940.1; AT1G75940.
    GeneIDi843927.
    KEGGiath:AT1G75940.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF037590 mRNA. Translation: AAC39504.1 .
    AC007396 Genomic DNA. Translation: AAF26759.2 . Sequence problems.
    CP002684 Genomic DNA. Translation: AEE35779.1 .
    AY074517 mRNA. Translation: AAL67131.1 .
    BT002735 mRNA. Translation: AAO22564.1 .
    PIRi T52048.
    RefSeqi NP_177722.1. NM_106244.2.
    UniGenei At.10790.

    3D structure databases

    ProteinModelPortali Q84WV2.
    SMRi Q84WV2. Positions 37-517.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT1G75940.1-P.

    Protein family/group databases

    CAZyi GH1. Glycoside Hydrolase Family 1.

    Proteomic databases

    PaxDbi Q84WV2.
    PRIDEi Q84WV2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G75940.1 ; AT1G75940.1 ; AT1G75940 .
    GeneIDi 843927.
    KEGGi ath:AT1G75940.

    Organism-specific databases

    TAIRi AT1G75940.

    Phylogenomic databases

    eggNOGi COG2723.
    HOGENOMi HOG000088630.
    InParanoidi Q84WV2.
    KOi K01188.
    OMAi HNDISAN.
    PhylomeDBi Q84WV2.

    Enzyme and pathway databases

    BioCyci ARA:AT1G75940-MONOMER.

    Gene expression databases

    Genevestigatori Q84WV2.

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    InterProi IPR001360. Glyco_hydro_1.
    IPR018120. Glyco_hydro_1_AS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    PANTHERi PTHR10353. PTHR10353. 1 hit.
    Pfami PF00232. Glyco_hydro_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00131. GLHYDRLASE1.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    PROSITEi PS00014. ER_TARGET. 1 hit.
    PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification, sequence analysis and expression studies of novel anther-specific genes of Arabidopsis thaliana."
      Rubinelli P., Hu Y., Ma H.
      Plant Mol. Biol. 37:607-619(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. Cited for: GENE FAMILY, NOMENCLATURE.

    Entry informationi

    Entry nameiBGL20_ARATH
    AccessioniPrimary (citable) accession number: Q84WV2
    Secondary accession number(s): O49117, Q8VXW3, Q9LQS3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 24, 2009
    Last sequence update: June 1, 2003
    Last modified: October 1, 2014
    This is version 80 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3