Q84WC6 (UBP7_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin carboxyl-terminal hydrolase 7
EC=3.4.19.12

Alternative name(s):
Deubiquitinating enzyme 7
Short name=AtUBP7
Ubiquitin thioesterase 7
Ubiquitin-specific-processing protease 7

Gene names

Name:	UBP7
Ordered Locus Names:	At3g21280
ORF Names:	MXL8.15

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	477 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins By similarity.
Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Subunit structure	Interacts with calmodulin (CaM). Ref.5
Sequence similarities	Belongs to the peptidase C19 family. Contains 1 ubiquitin-like domain.
Sequence caution	The sequence AEE76487.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened. The sequence BAB01721.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Ligand	Calmodulin-binding
Molecular function	Hydrolase Protease Thiol protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin thiolesterase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 477	477	Ubiquitin carboxyl-terminal hydrolase 7		PRO_0000313034
Regions
	Domain	2 – 77	76	Ubiquitin-like
	Region	171 – 190	20	Calmodulin-binding By similarity
Sites
	Active site	113	1	Nucleophile By similarity
	Active site	425	1	Proton acceptor By similarity
Experimental info
	Sequence conflict	209	1	S → F in AAG42752. Ref.1
	Sequence conflict	217	1	P → A in AAG42752. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q84WC6 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 82B469EE366B5E13	FASTA	477	53,516
	10 20 30 40 50 60 MLTVSVKWQK KVFESIEIDT SQPPFVFKAQ LYDLSGVPPE RQKIMVKGGL LKDDADWSTL 70 80 90 100 110 120 GLKNGQKLMM MGTADEIVKA PEKGPVFMED LPEEQQAANL GYSAGLVNLG NTCYMNSTMQ 130 140 150 160 170 180 CLISVPELKS ELSNYQSART KDVDQTSHML TVATRELFSE LDKSVKAVAP MPFWMVLQKK 190 200 210 220 230 240 YPQFAQLHNG NHMQQDAEEC WTQMLYTLSQ SLKLPSPSED PDAVKALFGL NLLNRLHCQE 250 260 270 280 290 300 SSEESSETES VFSLKCHISH EVNHLHEGLK HGLKGELEKT SPSLGRTAVY VKESLIDSLP 310 320 330 340 350 360 RYLTVQFVRF FWKRESNQKA KILRKVDYPL ELDIYDLCSE DLRKKLEAPR QKLRDIEGQK 370 380 390 400 410 420 LGLQASAKSS SKGDDVKMTD AEGSSNQSGE SSTGDQQEGA SPHMTGIYDL VSVLTHKGRS 430 440 450 460 470 ADSGHYVAWV KQESGKWVQY DDANTSLQRG EDIIKLSGGG DWHMAYIVMY KARLISM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The ubiquitin-specific protease family from Arabidopsis. AtUBP1 and 2 are required for the resistance to the amino acid analog canavanine." Yan N., Doelling J.H., Falbel T.G., Durski A.M., Vierstra R.D. Plant Physiol. 124:1828-1843(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY ORGANIZATION, NOMENCLATURE. Strain: cv. Columbia.
[2]	"Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones." Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S. DNA Res. 7:131-135(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Arabidopsis ubiquitin-specific protease 6 (AtUBP6) interacts with calmodulin." Moon B.C., Choi M.S., Kang Y.H., Kim M.C., Cheong M.S., Park C.Y., Yoo J.H., Koo S.C., Lee S.M., Lim C.O., Cho M.J., Chung W.S. FEBS Lett. 579:3885-3890(2005) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH CALMODULIN.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF302661 mRNA. Translation: AAG42752.1. AB023045 Genomic DNA. Translation: BAB01721.1. Sequence problems. CP002686 Genomic DNA. Translation: AEE76487.1. Different initiation. BT003992 mRNA. Translation: AAO42031.1.
IPI	IPI00527646.
RefSeq	NP_566680.2. NM_113023.3.
UniGene	At.8082.
3D structure databases
HSSP	HSSP built from PDB template 2AYN based on UniProtKB P54578.
ProteinModelPortal	Q84WC6.
SMR	Q84WC6. Positions 4-473.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT3G21280.1-P.
Protein family/group databases
MEROPS	C19.A05.
Proteomic databases
PaxDb	Q84WC6.
PRIDE	Q84WC6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	821682.
KEGG	ath:AT3G21280.
Organism-specific databases
TAIR	At3g21280.
Phylogenomic databases
eggNOG	NOG286607.
HOGENOM	HOG000202292.
InParanoid	Q84WC6.
KO	K11843.
PhylomeDB	Q84WC6.
Gene expression databases
ArrayExpress	Q84WC6.
Genevestigator	Q84WC6.
Family and domain databases
InterPro	IPR018200. Pept_C19ubi-hydrolase_C_CS. IPR001394. Peptidase_C19. IPR000626. Ubiquitin. IPR019954. Ubiquitin_CS. IPR019955. Ubiquitin_supergroup. [Graphical view]
Pfam	PF00240. ubiquitin. 1 hit. PF00443. UCH. 1 hit. [Graphical view]
SMART	SM00213. UBQ. 1 hit. [Graphical view]
PROSITE	PS00299. UBIQUITIN_1. 1 hit. PS50053. UBIQUITIN_2. 1 hit. PS00972. UCH_2_1. 1 hit. PS00973. UCH_2_2. 1 hit. PS50235. UCH_2_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UBP7_ARATH

Accession

Primary (citable) accession number: Q84WC6
Secondary accession number(s): F4IXI8, Q9FPT3, Q9LU31

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	June 1, 2003
Last modified:	May 1, 2013
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program