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Protein

Ubiquitin carboxyl-terminal hydrolase 7

Gene

UBP7

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei113NucleophilePROSITE-ProRule annotation1
Active sitei425Proton acceptorPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionCalmodulin-binding, Hydrolase, Protease, Thiol protease
Biological processUbl conjugation pathway

Enzyme and pathway databases

ReactomeiR-ATH-5689880 Ub-specific processing proteases

Protein family/group databases

MEROPSiC19.A05

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 7 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 7
Short name:
AtUBP7
Ubiquitin thioesterase 7
Ubiquitin-specific-processing protease 7
Gene namesi
Name:UBP7
Ordered Locus Names:At3g21280
ORF Names:MXL8.15
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G21280

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003130341 – 477Ubiquitin carboxyl-terminal hydrolase 7Add BLAST477

Proteomic databases

PaxDbiQ84WC6

Expressioni

Gene expression databases

ExpressionAtlasiQ84WC6 baseline and differential
GenevisibleiQ84WC6 AT

Interactioni

Subunit structurei

Interacts with calmodulin (CaM).1 Publication

GO - Molecular functioni

Protein-protein interaction databases

STRINGi3702.AT3G21280.1

Structurei

3D structure databases

ProteinModelPortaliQ84WC6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 77Ubiquitin-likePROSITE-ProRule annotationAdd BLAST76
Domaini104 – 473USPAdd BLAST370

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni171 – 190Calmodulin-bindingBy similarityAdd BLAST20

Sequence similaritiesi

Belongs to the peptidase C19 family.Curated

Phylogenomic databases

eggNOGiKOG1872 Eukaryota
ENOG410XP96 LUCA
HOGENOMiHOG000202292
InParanoidiQ84WC6
KOiK11843
OrthoDBiEOG09360955
PhylomeDBiQ84WC6

Family and domain databases

InterProiView protein in InterPro
IPR001394 Peptidase_C19_UCH
IPR029071 Ubiquitin-like_domsf
IPR019954 Ubiquitin_CS
IPR000626 Ubiquitin_dom
IPR018200 USP_CS
IPR028889 USP_dom
PfamiView protein in Pfam
PF00240 ubiquitin, 1 hit
PF00443 UCH, 1 hit
SMARTiView protein in SMART
SM00213 UBQ, 1 hit
SUPFAMiSSF54236 SSF54236, 1 hit
PROSITEiView protein in PROSITE
PS00299 UBIQUITIN_1, 1 hit
PS50053 UBIQUITIN_2, 1 hit
PS00972 USP_1, 1 hit
PS00973 USP_2, 1 hit
PS50235 USP_3, 1 hit

Sequencei

Sequence statusi: Complete.

Q84WC6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLTVSVKWQK KVFESIEIDT SQPPFVFKAQ LYDLSGVPPE RQKIMVKGGL
60 70 80 90 100
LKDDADWSTL GLKNGQKLMM MGTADEIVKA PEKGPVFMED LPEEQQAANL
110 120 130 140 150
GYSAGLVNLG NTCYMNSTMQ CLISVPELKS ELSNYQSART KDVDQTSHML
160 170 180 190 200
TVATRELFSE LDKSVKAVAP MPFWMVLQKK YPQFAQLHNG NHMQQDAEEC
210 220 230 240 250
WTQMLYTLSQ SLKLPSPSED PDAVKALFGL NLLNRLHCQE SSEESSETES
260 270 280 290 300
VFSLKCHISH EVNHLHEGLK HGLKGELEKT SPSLGRTAVY VKESLIDSLP
310 320 330 340 350
RYLTVQFVRF FWKRESNQKA KILRKVDYPL ELDIYDLCSE DLRKKLEAPR
360 370 380 390 400
QKLRDIEGQK LGLQASAKSS SKGDDVKMTD AEGSSNQSGE SSTGDQQEGA
410 420 430 440 450
SPHMTGIYDL VSVLTHKGRS ADSGHYVAWV KQESGKWVQY DDANTSLQRG
460 470
EDIIKLSGGG DWHMAYIVMY KARLISM
Length:477
Mass (Da):53,516
Last modified:June 1, 2003 - v1
Checksum:i82B469EE366B5E13
GO

Sequence cautioni

The sequence AEE76487 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence BAB01721 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti209S → F in AAG42752 (PubMed:11115897).Curated1
Sequence conflicti217P → A in AAG42752 (PubMed:11115897).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF302661 mRNA Translation: AAG42752.1
AB023045 Genomic DNA Translation: BAB01721.1 Sequence problems.
CP002686 Genomic DNA Translation: AEE76487.1 Different initiation.
BT003992 mRNA Translation: AAO42031.1
RefSeqiNP_566680.2, NM_113023.4
UniGeneiAt.8082

Genome annotation databases

EnsemblPlantsiAT3G21280.1; AT3G21280.1; AT3G21280
GeneIDi821682
GrameneiAT3G21280.1; AT3G21280.1; AT3G21280
KEGGiath:AT3G21280

Similar proteinsi

Entry informationi

Entry nameiUBP7_ARATH
AccessioniPrimary (citable) accession number: Q84WC6
Secondary accession number(s): F4IXI8, Q9FPT3, Q9LU31
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: June 1, 2003
Last modified: April 25, 2018
This is version 94 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health