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Q84WC6 (UBP7_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin carboxyl-terminal hydrolase 7

EC=3.4.19.12
Alternative name(s):
Deubiquitinating enzyme 7
Short name=AtUBP7
Ubiquitin thioesterase 7
Ubiquitin-specific-processing protease 7
Gene names
Name:UBP7
Ordered Locus Names:At3g21280
ORF Names:MXL8.15
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length477 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins By similarity.

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Subunit structure

Interacts with calmodulin (CaM). Ref.5

Sequence similarities

Belongs to the peptidase C19 family.

Contains 1 ubiquitin-like domain.

Contains 1 USP domain.

Sequence caution

The sequence AEE76487.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

The sequence BAB01721.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
   Biological processUbl conjugation pathway
   LigandCalmodulin-binding
   Molecular functionHydrolase
Protease
Thiol protease
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processubiquitin-dependent protein catabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular_functioncysteine-type peptidase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 477477Ubiquitin carboxyl-terminal hydrolase 7
PRO_0000313034

Regions

Domain2 – 7776Ubiquitin-like
Domain104 – 473370USP
Region171 – 19020Calmodulin-binding By similarity

Sites

Active site1131Nucleophile By similarity
Active site4251Proton acceptor By similarity

Experimental info

Sequence conflict2091S → F in AAG42752. Ref.1
Sequence conflict2171P → A in AAG42752. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q84WC6 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 82B469EE366B5E13

FASTA47753,516
        10         20         30         40         50         60 
MLTVSVKWQK KVFESIEIDT SQPPFVFKAQ LYDLSGVPPE RQKIMVKGGL LKDDADWSTL 

        70         80         90        100        110        120 
GLKNGQKLMM MGTADEIVKA PEKGPVFMED LPEEQQAANL GYSAGLVNLG NTCYMNSTMQ 

       130        140        150        160        170        180 
CLISVPELKS ELSNYQSART KDVDQTSHML TVATRELFSE LDKSVKAVAP MPFWMVLQKK 

       190        200        210        220        230        240 
YPQFAQLHNG NHMQQDAEEC WTQMLYTLSQ SLKLPSPSED PDAVKALFGL NLLNRLHCQE 

       250        260        270        280        290        300 
SSEESSETES VFSLKCHISH EVNHLHEGLK HGLKGELEKT SPSLGRTAVY VKESLIDSLP 

       310        320        330        340        350        360 
RYLTVQFVRF FWKRESNQKA KILRKVDYPL ELDIYDLCSE DLRKKLEAPR QKLRDIEGQK 

       370        380        390        400        410        420 
LGLQASAKSS SKGDDVKMTD AEGSSNQSGE SSTGDQQEGA SPHMTGIYDL VSVLTHKGRS 

       430        440        450        460        470 
ADSGHYVAWV KQESGKWVQY DDANTSLQRG EDIIKLSGGG DWHMAYIVMY KARLISM 

« Hide

References

« Hide 'large scale' references
[1]"The ubiquitin-specific protease family from Arabidopsis. AtUBP1 and 2 are required for the resistance to the amino acid analog canavanine."
Yan N., Doelling J.H., Falbel T.G., Durski A.M., Vierstra R.D.
Plant Physiol. 124:1828-1843(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY ORGANIZATION, NOMENCLATURE.
Strain: cv. Columbia.
[2]"Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones."
Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.
DNA Res. 7:131-135(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Arabidopsis ubiquitin-specific protease 6 (AtUBP6) interacts with calmodulin."
Moon B.C., Choi M.S., Kang Y.H., Kim M.C., Cheong M.S., Park C.Y., Yoo J.H., Koo S.C., Lee S.M., Lim C.O., Cho M.J., Chung W.S.
FEBS Lett. 579:3885-3890(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH CALMODULIN.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF302661 mRNA. Translation: AAG42752.1.
AB023045 Genomic DNA. Translation: BAB01721.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE76487.1. Different initiation.
BT003992 mRNA. Translation: AAO42031.1.
RefSeqNP_566680.2. NM_113023.3.
UniGeneAt.8082.

3D structure databases

ProteinModelPortalQ84WC6.
SMRQ84WC6. Positions 1-473.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING3702.AT3G21280.1-P.

Protein family/group databases

MEROPSC19.A05.

Proteomic databases

PaxDbQ84WC6.
PRIDEQ84WC6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID821682.
KEGGath:AT3G21280.

Organism-specific databases

TAIRAT3G21280.

Phylogenomic databases

eggNOGNOG286607.
HOGENOMHOG000202292.
InParanoidQ84WC6.
KOK11843.
PhylomeDBQ84WC6.

Enzyme and pathway databases

BioCycARA:AT3G21280-MONOMER.

Gene expression databases

GenevestigatorQ84WC6.

Family and domain databases

InterProIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR000626. Ubiquitin-like.
IPR019954. Ubiquitin_CS.
IPR028889. UCH/PAN2.
[Graphical view]
PfamPF00240. ubiquitin. 1 hit.
PF00443. UCH. 1 hit.
[Graphical view]
SMARTSM00213. UBQ. 1 hit.
[Graphical view]
PROSITEPS00299. UBIQUITIN_1. 1 hit.
PS50053. UBIQUITIN_2. 1 hit.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameUBP7_ARATH
AccessionPrimary (citable) accession number: Q84WC6
Secondary accession number(s): F4IXI8, Q9FPT3, Q9LU31
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: June 1, 2003
Last modified: April 16, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names