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Protein

Ubiquitin carboxyl-terminal hydrolase 7

Gene

UBP7

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei113NucleophilePROSITE-ProRule annotation1
Active sitei425Proton acceptorPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Calmodulin-binding

Enzyme and pathway databases

BioCyciARA:AT3G21280-MONOMER.
ReactomeiR-ATH-5689880. Ub-specific processing proteases.

Protein family/group databases

MEROPSiC19.A05.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 7 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 7
Short name:
AtUBP7
Ubiquitin thioesterase 7
Ubiquitin-specific-processing protease 7
Gene namesi
Name:UBP7
Ordered Locus Names:At3g21280
ORF Names:MXL8.15
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G21280.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003130341 – 477Ubiquitin carboxyl-terminal hydrolase 7Add BLAST477

Proteomic databases

PaxDbiQ84WC6.

Expressioni

Gene expression databases

GenevisibleiQ84WC6. AT.

Interactioni

Subunit structurei

Interacts with calmodulin (CaM).1 Publication

Protein-protein interaction databases

STRINGi3702.AT3G21280.1.

Structurei

3D structure databases

ProteinModelPortaliQ84WC6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 77Ubiquitin-likePROSITE-ProRule annotationAdd BLAST76
Domaini104 – 473USPAdd BLAST370

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni171 – 190Calmodulin-bindingBy similarityAdd BLAST20

Sequence similaritiesi

Belongs to the peptidase C19 family.Curated
Contains 1 ubiquitin-like domain.PROSITE-ProRule annotation
Contains 1 USP domain.Curated

Phylogenomic databases

eggNOGiKOG1872. Eukaryota.
ENOG410XP96. LUCA.
HOGENOMiHOG000202292.
InParanoidiQ84WC6.
KOiK11843.
OrthoDBiEOG09360955.
PhylomeDBiQ84WC6.

Family and domain databases

InterProiIPR001394. Peptidase_C19_UCH.
IPR029071. Ubiquitin-rel_dom.
IPR019954. Ubiquitin_CS.
IPR000626. Ubiquitin_dom.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF00240. ubiquitin. 1 hit.
PF00443. UCH. 1 hit.
[Graphical view]
SMARTiSM00213. UBQ. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
PROSITEiPS00299. UBIQUITIN_1. 1 hit.
PS50053. UBIQUITIN_2. 1 hit.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q84WC6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLTVSVKWQK KVFESIEIDT SQPPFVFKAQ LYDLSGVPPE RQKIMVKGGL
60 70 80 90 100
LKDDADWSTL GLKNGQKLMM MGTADEIVKA PEKGPVFMED LPEEQQAANL
110 120 130 140 150
GYSAGLVNLG NTCYMNSTMQ CLISVPELKS ELSNYQSART KDVDQTSHML
160 170 180 190 200
TVATRELFSE LDKSVKAVAP MPFWMVLQKK YPQFAQLHNG NHMQQDAEEC
210 220 230 240 250
WTQMLYTLSQ SLKLPSPSED PDAVKALFGL NLLNRLHCQE SSEESSETES
260 270 280 290 300
VFSLKCHISH EVNHLHEGLK HGLKGELEKT SPSLGRTAVY VKESLIDSLP
310 320 330 340 350
RYLTVQFVRF FWKRESNQKA KILRKVDYPL ELDIYDLCSE DLRKKLEAPR
360 370 380 390 400
QKLRDIEGQK LGLQASAKSS SKGDDVKMTD AEGSSNQSGE SSTGDQQEGA
410 420 430 440 450
SPHMTGIYDL VSVLTHKGRS ADSGHYVAWV KQESGKWVQY DDANTSLQRG
460 470
EDIIKLSGGG DWHMAYIVMY KARLISM
Length:477
Mass (Da):53,516
Last modified:June 1, 2003 - v1
Checksum:i82B469EE366B5E13
GO

Sequence cautioni

The sequence AEE76487 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence BAB01721 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti209S → F in AAG42752 (PubMed:11115897).Curated1
Sequence conflicti217P → A in AAG42752 (PubMed:11115897).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF302661 mRNA. Translation: AAG42752.1.
AB023045 Genomic DNA. Translation: BAB01721.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE76487.1. Different initiation.
BT003992 mRNA. Translation: AAO42031.1.
RefSeqiNP_566680.2. NM_113023.4.
UniGeneiAt.8082.

Genome annotation databases

EnsemblPlantsiAT3G21280.1; AT3G21280.1; AT3G21280.
GeneIDi821682.
GrameneiAT3G21280.1; AT3G21280.1; AT3G21280.
KEGGiath:AT3G21280.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF302661 mRNA. Translation: AAG42752.1.
AB023045 Genomic DNA. Translation: BAB01721.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE76487.1. Different initiation.
BT003992 mRNA. Translation: AAO42031.1.
RefSeqiNP_566680.2. NM_113023.4.
UniGeneiAt.8082.

3D structure databases

ProteinModelPortaliQ84WC6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT3G21280.1.

Protein family/group databases

MEROPSiC19.A05.

Proteomic databases

PaxDbiQ84WC6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G21280.1; AT3G21280.1; AT3G21280.
GeneIDi821682.
GrameneiAT3G21280.1; AT3G21280.1; AT3G21280.
KEGGiath:AT3G21280.

Organism-specific databases

TAIRiAT3G21280.

Phylogenomic databases

eggNOGiKOG1872. Eukaryota.
ENOG410XP96. LUCA.
HOGENOMiHOG000202292.
InParanoidiQ84WC6.
KOiK11843.
OrthoDBiEOG09360955.
PhylomeDBiQ84WC6.

Enzyme and pathway databases

BioCyciARA:AT3G21280-MONOMER.
ReactomeiR-ATH-5689880. Ub-specific processing proteases.

Miscellaneous databases

PROiQ84WC6.

Gene expression databases

GenevisibleiQ84WC6. AT.

Family and domain databases

InterProiIPR001394. Peptidase_C19_UCH.
IPR029071. Ubiquitin-rel_dom.
IPR019954. Ubiquitin_CS.
IPR000626. Ubiquitin_dom.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF00240. ubiquitin. 1 hit.
PF00443. UCH. 1 hit.
[Graphical view]
SMARTiSM00213. UBQ. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
PROSITEiPS00299. UBIQUITIN_1. 1 hit.
PS50053. UBIQUITIN_2. 1 hit.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUBP7_ARATH
AccessioniPrimary (citable) accession number: Q84WC6
Secondary accession number(s): F4IXI8, Q9FPT3, Q9LU31
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: June 1, 2003
Last modified: November 30, 2016
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.