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Q84WC6

- UBP7_ARATH

UniProt

Q84WC6 - UBP7_ARATH

Protein

Ubiquitin carboxyl-terminal hydrolase 7

Gene

UBP7

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 73 (01 Oct 2014)
      Sequence version 1 (01 Jun 2003)
      Previous versions | rss
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    Functioni

    Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins By similarity.By similarity

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei113 – 1131NucleophilePROSITE-ProRule annotation
    Active sitei425 – 4251Proton acceptorPROSITE-ProRule annotation

    GO - Molecular functioni

    1. cysteine-type peptidase activity Source: UniProtKB-KW
    2. ubiquitinyl hydrolase activity Source: InterPro

    GO - Biological processi

    1. ubiquitin-dependent protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Ubl conjugation pathway

    Keywords - Ligandi

    Calmodulin-binding

    Enzyme and pathway databases

    BioCyciARA:AT3G21280-MONOMER.

    Protein family/group databases

    MEROPSiC19.A05.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin carboxyl-terminal hydrolase 7 (EC:3.4.19.12)
    Alternative name(s):
    Deubiquitinating enzyme 7
    Short name:
    AtUBP7
    Ubiquitin thioesterase 7
    Ubiquitin-specific-processing protease 7
    Gene namesi
    Name:UBP7
    Ordered Locus Names:At3g21280
    ORF Names:MXL8.15
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 3

    Organism-specific databases

    TAIRiAT3G21280.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 477477Ubiquitin carboxyl-terminal hydrolase 7PRO_0000313034Add
    BLAST

    Proteomic databases

    PaxDbiQ84WC6.
    PRIDEiQ84WC6.

    Expressioni

    Gene expression databases

    GenevestigatoriQ84WC6.

    Interactioni

    Subunit structurei

    Interacts with calmodulin (CaM).1 Publication

    Protein-protein interaction databases

    STRINGi3702.AT3G21280.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ84WC6.
    SMRiQ84WC6. Positions 4-473.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini2 – 7776Ubiquitin-likePROSITE-ProRule annotationAdd
    BLAST
    Domaini104 – 473370USPAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni171 – 19020Calmodulin-bindingBy similarityAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase C19 family.Curated
    Contains 1 ubiquitin-like domain.PROSITE-ProRule annotation
    Contains 1 USP domain.Curated

    Phylogenomic databases

    eggNOGiNOG286607.
    HOGENOMiHOG000202292.
    InParanoidiQ84WC6.
    KOiK11843.
    PhylomeDBiQ84WC6.

    Family and domain databases

    InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR000626. Ubiquitin-like.
    IPR029071. Ubiquitin-rel_dom.
    IPR019954. Ubiquitin_CS.
    IPR028889. UCH/PAN2.
    [Graphical view]
    PfamiPF00240. ubiquitin. 1 hit.
    PF00443. UCH. 1 hit.
    [Graphical view]
    SMARTiSM00213. UBQ. 1 hit.
    [Graphical view]
    SUPFAMiSSF54236. SSF54236. 1 hit.
    PROSITEiPS00299. UBIQUITIN_1. 1 hit.
    PS50053. UBIQUITIN_2. 1 hit.
    PS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q84WC6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLTVSVKWQK KVFESIEIDT SQPPFVFKAQ LYDLSGVPPE RQKIMVKGGL    50
    LKDDADWSTL GLKNGQKLMM MGTADEIVKA PEKGPVFMED LPEEQQAANL 100
    GYSAGLVNLG NTCYMNSTMQ CLISVPELKS ELSNYQSART KDVDQTSHML 150
    TVATRELFSE LDKSVKAVAP MPFWMVLQKK YPQFAQLHNG NHMQQDAEEC 200
    WTQMLYTLSQ SLKLPSPSED PDAVKALFGL NLLNRLHCQE SSEESSETES 250
    VFSLKCHISH EVNHLHEGLK HGLKGELEKT SPSLGRTAVY VKESLIDSLP 300
    RYLTVQFVRF FWKRESNQKA KILRKVDYPL ELDIYDLCSE DLRKKLEAPR 350
    QKLRDIEGQK LGLQASAKSS SKGDDVKMTD AEGSSNQSGE SSTGDQQEGA 400
    SPHMTGIYDL VSVLTHKGRS ADSGHYVAWV KQESGKWVQY DDANTSLQRG 450
    EDIIKLSGGG DWHMAYIVMY KARLISM 477
    Length:477
    Mass (Da):53,516
    Last modified:June 1, 2003 - v1
    Checksum:i82B469EE366B5E13
    GO

    Sequence cautioni

    The sequence AEE76487.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.
    The sequence BAB01721.1 differs from that shown. Reason: Erroneous gene model prediction.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti209 – 2091S → F in AAG42752. (PubMed:11115897)Curated
    Sequence conflicti217 – 2171P → A in AAG42752. (PubMed:11115897)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF302661 mRNA. Translation: AAG42752.1.
    AB023045 Genomic DNA. Translation: BAB01721.1. Sequence problems.
    CP002686 Genomic DNA. Translation: AEE76487.1. Different initiation.
    BT003992 mRNA. Translation: AAO42031.1.
    RefSeqiNP_566680.2. NM_113023.3.
    UniGeneiAt.8082.

    Genome annotation databases

    EnsemblPlantsiAT3G21280.1; AT3G21280.1; AT3G21280.
    GeneIDi821682.
    KEGGiath:AT3G21280.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF302661 mRNA. Translation: AAG42752.1 .
    AB023045 Genomic DNA. Translation: BAB01721.1 . Sequence problems.
    CP002686 Genomic DNA. Translation: AEE76487.1 . Different initiation.
    BT003992 mRNA. Translation: AAO42031.1 .
    RefSeqi NP_566680.2. NM_113023.3.
    UniGenei At.8082.

    3D structure databases

    ProteinModelPortali Q84WC6.
    SMRi Q84WC6. Positions 4-473.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT3G21280.1-P.

    Protein family/group databases

    MEROPSi C19.A05.

    Proteomic databases

    PaxDbi Q84WC6.
    PRIDEi Q84WC6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT3G21280.1 ; AT3G21280.1 ; AT3G21280 .
    GeneIDi 821682.
    KEGGi ath:AT3G21280.

    Organism-specific databases

    TAIRi AT3G21280.

    Phylogenomic databases

    eggNOGi NOG286607.
    HOGENOMi HOG000202292.
    InParanoidi Q84WC6.
    KOi K11843.
    PhylomeDBi Q84WC6.

    Enzyme and pathway databases

    BioCyci ARA:AT3G21280-MONOMER.

    Gene expression databases

    Genevestigatori Q84WC6.

    Family and domain databases

    InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR000626. Ubiquitin-like.
    IPR029071. Ubiquitin-rel_dom.
    IPR019954. Ubiquitin_CS.
    IPR028889. UCH/PAN2.
    [Graphical view ]
    Pfami PF00240. ubiquitin. 1 hit.
    PF00443. UCH. 1 hit.
    [Graphical view ]
    SMARTi SM00213. UBQ. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54236. SSF54236. 1 hit.
    PROSITEi PS00299. UBIQUITIN_1. 1 hit.
    PS50053. UBIQUITIN_2. 1 hit.
    PS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The ubiquitin-specific protease family from Arabidopsis. AtUBP1 and 2 are required for the resistance to the amino acid analog canavanine."
      Yan N., Doelling J.H., Falbel T.G., Durski A.M., Vierstra R.D.
      Plant Physiol. 124:1828-1843(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY ORGANIZATION, NOMENCLATURE.
      Strain: cv. Columbia.
    2. "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones."
      Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.
      DNA Res. 7:131-135(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Arabidopsis ubiquitin-specific protease 6 (AtUBP6) interacts with calmodulin."
      Moon B.C., Choi M.S., Kang Y.H., Kim M.C., Cheong M.S., Park C.Y., Yoo J.H., Koo S.C., Lee S.M., Lim C.O., Cho M.J., Chung W.S.
      FEBS Lett. 579:3885-3890(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CALMODULIN.

    Entry informationi

    Entry nameiUBP7_ARATH
    AccessioniPrimary (citable) accession number: Q84WC6
    Secondary accession number(s): F4IXI8, Q9FPT3, Q9LU31
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: June 1, 2003
    Last modified: October 1, 2014
    This is version 73 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3