Q84W92 (ANM13_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable histone-arginine methyltransferase 1.3
Short name=AtPRMT13
EC=2.1.1.-
EC=2.1.1.125

Alternative name(s):
Coactivator-associated methyltransferase 1A
Protein arginine N-methyltransferase 4B
Short name=AtPRMT4B

Gene names

Name:	PRMT13
Synonyms:	CARM1A, PRMT4B
Ordered Locus Names:	At3g06930
ORF Names:	F17A9.8

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	535 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Methylates (mono- and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in several proteins involved in DNA packaging, transcription regulation, and mRNA stability. Recruited to promoters upon gene activation, methylates histone H3 and activates transcription via chromatin remodeling By similarity.
Catalytic activity	S-adenosyl-L-methionine + arginine-[histone] = S-adenosyl-L-homocysteine + N(omega)-methyl-arginine-[histone].
Subcellular location	Nucleus By similarity. Cytoplasm By similarity.
Sequence similarities	Belongs to the protein arginine N-methyltransferase family.
Sequence caution	The sequence AAF26997.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence BAF00156.1 differs from that shown. Reason: Chimeric cDNA. Its C-terminal part is derived from gene At1g80500, which encoded for an intracellular transporter.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing
Ligand	S-adenosyl-L-methionine
Molecular function	Chromatin regulator Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	histone H3-R17 methylation Inferred from genetic interaction. Source: TAIR histone H3-R2 methylation Inferred from direct assay. Source: TAIR histone H3-R26 methylation Inferred from direct assay. Source: TAIR regulation of flower development Inferred from genetic interaction. Source: TAIR regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW vegetative to reproductive phase transition of meristem Inferred from genetic interaction. Source: TAIR
Cellular component	cytoplasm Inferred from direct assay. Source: TAIR nucleus Inferred from direct assay. Source: TAIR
Molecular function	[myelin basic protein]-arginine N-methyltransferase activity Inferred from direct assay. Source: TAIR histone-arginine N-methyltransferase activity Inferred from direct assay. Source: TAIR protein heterodimerization activity Inferred from physical interaction. Source: TAIR protein homodimerization activity Inferred from physical interaction. Source: TAIR protein-arginine omega-N asymmetric methyltransferase activity Inferred from direct assay. Source: TAIR protein-arginine omega-N monomethyltransferase activity Inferred from direct assay. Source: TAIR
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q84W92-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q84W92-3)

The sequence of this isoform differs from the canonical sequence as follows:
     510-510: Missing.

Note: May be due to competing acceptor splice site. No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 535	535	Probable histone-arginine methyltransferase 1.3		PRO_0000294004
Regions
	Compositional bias	23 – 31	9	Poly-Ser
	Compositional bias	81 – 84	4	Poly-Ser
Sites
	Binding site	158	1	S-adenosyl-L-methionine By similarity
	Binding site	167	1	S-adenosyl-L-methionine By similarity
	Binding site	191	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	213	1	S-adenosyl-L-methionine By similarity
	Binding site	243	1	S-adenosyl-L-methionine By similarity
	Binding site	271	1	S-adenosyl-L-methionine By similarity
Natural variations
	Alternative sequence	510	1	Missing in isoform 2.		VSP_027461
Experimental info
	Sequence conflict	127	1	G → V in BAF00156. Ref.5
	Sequence conflict	374	1	P → Q in AAO42127. Ref.3
	Sequence conflict	377	1	F → L in AAO42127. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified August 21, 2007. Version 3. Checksum: E70BF4F1F6606888	FASTA	535	59,915
	10 20 30 40 50 60 MEVSSVKKLE QLEYSLESVT DLSSSSVSSS SPAVATFSYV DGVTELRFLQ SDSTHCFNFD 70 80 90 100 110 120 LASAQLFKLG PVHFICVSDG SSSSEEKSFS KGVNIKFKNE KDSKDFCESF EEWRNDSVVQ 130 140 150 160 170 180 GSSLQNGTVS ANKSKFDNKI EASSAKMYFH YYGQLLHQQN MLQDYVRTGT YYAAVMENHS 190 200 210 220 230 240 DFAGRVVVDV GAGSGILSMF AAQAGAKHVY AVEASEMAEY ARKLIAGNPL FADRITVIKG 250 260 270 280 290 300 KVEDIELPEK ADILISEPMG TLLVNERMLE SYVIARDRFM TPKGKMFPTV GRIHMAPFSD 310 320 330 340 350 360 EFLFIEMANK AMFWQQQNYY GVDLTPLYGS AHQGYFSQPV VDAFDPRLLV ASPMFHMIDF 370 380 390 400 410 420 TQMKEEDFYE IDIPLKFTAS MCTRMHGLAC WFDVLFDGST VQRWLTTAPG APTTHWYQIR 430 440 450 460 470 480 CVLSQPIYVM AGQEITGRLH LIAHSAQSYT IDLTLSAKMW GPGASQGGIL QSSTCKFDLK 490 500 510 520 530 EPYYRMSQPQ AYPVAQEPPL QPQPELSTQQ DIQTPNDELE EELLQQLPQN PSAQL
	Isoform 2 [UniParc]. Checksum: 3F4B90B778FC7C9C	FASTA	534	59,787
	10 20 30 40 50 60 MEVSSVKKLE QLEYSLESVT DLSSSSVSSS SPAVATFSYV DGVTELRFLQ SDSTHCFNFD 70 80 90 100 110 120 LASAQLFKLG PVHFICVSDG SSSSEEKSFS KGVNIKFKNE KDSKDFCESF EEWRNDSVVQ 130 140 150 160 170 180 GSSLQNGTVS ANKSKFDNKI EASSAKMYFH YYGQLLHQQN MLQDYVRTGT YYAAVMENHS 190 200 210 220 230 240 DFAGRVVVDV GAGSGILSMF AAQAGAKHVY AVEASEMAEY ARKLIAGNPL FADRITVIKG 250 260 270 280 290 300 KVEDIELPEK ADILISEPMG TLLVNERMLE SYVIARDRFM TPKGKMFPTV GRIHMAPFSD 310 320 330 340 350 360 EFLFIEMANK AMFWQQQNYY GVDLTPLYGS AHQGYFSQPV VDAFDPRLLV ASPMFHMIDF 370 380 390 400 410 420 TQMKEEDFYE IDIPLKFTAS MCTRMHGLAC WFDVLFDGST VQRWLTTAPG APTTHWYQIR 430 440 450 460 470 480 CVLSQPIYVM AGQEITGRLH LIAHSAQSYT IDLTLSAKMW GPGASQGGIL QSSTCKFDLK 490 500 510 520 530 EPYYRMSQPQ AYPVAQEPPL QPQPELSTQD IQTPNDELEE ELLQQLPQNP SAQL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana." Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. Nature 408:820-822(2000) [PubMed: 11130713] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: cv. Columbia.
[4]	"Arabidopsis ORF clones." Cheuk R.F., Chen H., Kim C.J., Shinn P., Ecker J.R. Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: cv. Columbia.
[5]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[6]	"Protein arginine methyltransferases: evolution and assessment of their pharmacological and therapeutic potential." Krause C.D., Yang Z.-H., Kim Y.-S., Lee J.-H., Cook J.R., Pestka S. Pharmacol. Ther. 113:50-87(2007) [PubMed: 17005254] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Web resources

The Chromatin Database (ChromDB)

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC016827 Genomic DNA. Translation: AAF26997.1. Sequence problems. CP002686 Genomic DNA. Translation: AEE74477.1. CP002686 Genomic DNA. Translation: AEE74478.1. BT004102 mRNA. Translation: AAO42127.1. BT015414 mRNA. Translation: AAU05537.1. AK228202 mRNA. Translation: BAF00156.1. Sequence problems.
IPI	IPI00517510. IPI00866771.
RefSeq	NP_187349.2. NM_111573.4. NP_850528.1. NM_180197.2.
UniGene	At.18748.
3D structure databases
HSSP	HSSP built from PDB template 1G6Q based on UniProtKB P38074.
ProteinModelPortal	Q84W92.
SMR	Q84W92. Positions 134-487.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q84W92. 1 interaction.
STRING	Q84W92.
Proteomic databases
PRIDE	Q84W92.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT3G06930.2; AT3G06930.2; AT3G06930.
GeneID	819878.
GenomeReviews	Gene locus AT3G06930 in contig BA000014_GR.
KEGG	ath:AT3G06930.
NMPDR	fig|3702.1.peg.12714.
Organism-specific databases
TAIR	At3g06930.
Phylogenomic databases
eggNOG	KOG1500.
GeneTree	EPGT00070000029771.
HOGENOM	HBG717391.
InParanoid	Q84W92.
OMA	MSQPQAY.
PhylomeDB	Q84W92.
ProtClustDB	CLSN2690752.
Gene expression databases
ArrayExpress	Q84W92.
Genevestigator	Q84W92.
Family and domain databases
InterPro	IPR007857. Skb1_MeTrfase. [Graphical view]
KO	K05931.
Pfam	PF05185. PRMT5. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ANM13_ARATH

Accession

Primary (citable) accession number: Q84W92
Secondary accession number(s): Q0WRU3, Q66GI8, Q9M906

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	August 21, 2007
Last modified:	December 14, 2011
This is version 58 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program