ID   RFS1_ARATH              Reviewed;         754 AA.
AC   Q84VX0; Q9LFZ7;
DT   09-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   27-MAR-2024, entry version 114.
DE   RecName: Full=Probable galactinol--sucrose galactosyltransferase 1;
DE            EC=2.4.1.82;
DE   AltName: Full=Protein SEED IMBIBITION 1;
DE   AltName: Full=Raffinose synthase 1;
GN   Name=RFS1; Synonyms=RS1, SIP1; OrderedLocusNames=At1g55740;
GN   ORFNames=F20N2.12, F20N2.14;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130712; DOI=10.1038/35048500;
RA   Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA   Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA   Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA   Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA   Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA   Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA   Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA   Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA   Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA   Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA   Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA   Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA   Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA   Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA   Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT   "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL   Nature 408:816-820(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA   Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA   Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA   Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA   Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA   Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA   Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA   Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA   Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA   Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA   Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA   Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RA   Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA   Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA   Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA   Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA   Shinozaki K.;
RT   "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL   Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   INDUCTION BY OXIDATIVE STRESS, GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=18502973; DOI=10.1104/pp.108.122465;
RA   Nishizawa A., Yabuta Y., Shigeoka S.;
RT   "Galactinol and raffinose constitute a novel function to protect plants
RT   from oxidative damage.";
RL   Plant Physiol. 147:1251-1263(2008).
CC   -!- FUNCTION: Transglycosidase operating by a ping-pong reaction mechanism.
CC       Involved in the synthesis of raffinose, a major soluble carbohydrate in
CC       seeds, roots and tubers (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=alpha-D-galactosyl-(1->3)-1D-myo-inositol + sucrose = myo-
CC         inositol + raffinose; Xref=Rhea:RHEA:20161, ChEBI:CHEBI:16634,
CC         ChEBI:CHEBI:17268, ChEBI:CHEBI:17505, ChEBI:CHEBI:17992; EC=2.4.1.82;
CC   -!- INDUCTION: Not induced by oxidative stress.
CC       {ECO:0000269|PubMed:18502973}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolases 36 family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAF79504.1; Type=Erroneous gene model prediction; Note=The predicted gene has been split into 2 genes: At1g55730 and At1g55740.; Evidence={ECO:0000305};
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DR   EMBL; AC002328; AAF79504.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP002684; AEE33292.1; -; Genomic_DNA.
DR   EMBL; BT004640; AAO42886.1; -; mRNA.
DR   EMBL; AK227977; BAE99943.1; -; mRNA.
DR   PIR; C96599; C96599.
DR   RefSeq; NP_175970.1; NM_104450.4.
DR   AlphaFoldDB; Q84VX0; -.
DR   SMR; Q84VX0; -.
DR   BioGRID; 27248; 1.
DR   IntAct; Q84VX0; 1.
DR   STRING; 3702.Q84VX0; -.
DR   CAZy; GH36; Glycoside Hydrolase Family 36.
DR   PaxDb; 3702-AT1G55740-1; -.
DR   ProteomicsDB; 236923; -.
DR   EnsemblPlants; AT1G55740.1; AT1G55740.1; AT1G55740.
DR   GeneID; 842023; -.
DR   Gramene; AT1G55740.1; AT1G55740.1; AT1G55740.
DR   KEGG; ath:AT1G55740; -.
DR   Araport; AT1G55740; -.
DR   TAIR; AT1G55740; SIP1.
DR   eggNOG; ENOG502QPVE; Eukaryota.
DR   HOGENOM; CLU_007066_0_0_1; -.
DR   InParanoid; Q84VX0; -.
DR   OMA; QTFSHRD; -.
DR   OrthoDB; 214587at2759; -.
DR   PhylomeDB; Q84VX0; -.
DR   BioCyc; ARA:AT1G55740-MONOMER; -.
DR   BRENDA; 2.4.1.82; 399.
DR   PRO; PR:Q84VX0; -.
DR   Proteomes; UP000006548; Chromosome 1.
DR   ExpressionAtlas; Q84VX0; baseline and differential.
DR   GO; GO:0047274; F:galactinol-sucrose galactosyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:UniProtKB-KW.
DR   Gene3D; 3.20.20.70; Aldolase class I; 1.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   InterPro; IPR008811; Glycosyl_hydrolases_36.
DR   PANTHER; PTHR31268; -; 1.
DR   PANTHER; PTHR31268:SF29; GALACTINOL--SUCROSE GALACTOSYLTRANSFERASE 1-RELATED; 1.
DR   Pfam; PF05691; Raffinose_syn; 1.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR   Genevisible; Q84VX0; AT.
PE   2: Evidence at transcript level;
KW   Carbohydrate metabolism; Glycosyltransferase; Reference proteome;
KW   Transferase.
FT   CHAIN           1..754
FT                   /note="Probable galactinol--sucrose galactosyltransferase
FT                   1"
FT                   /id="PRO_0000270157"
SQ   SEQUENCE   754 AA;  83477 MW;  C57DB5076DADA8AD CRC64;
     MTVGAGISVT DSDLVVLGHR VLHGVPENVL VTPASGNALI DGAFIGVTSD QTGSHRVFSL
     GKLEDLRFMC VFRFKLWWMT QRMGTNGKEI PCETQFLIVE ANQGSDLGGR DQSSSYVVFL
     PILEGDFRAV LQGNEANELE ICLESGDPTV DQFEGSHLVF VAAGSDPFDV ITKAVKAVEQ
     HLQTFSHRER KKMPDMLNWF GWCTWDAFYT NVTAKDVKQG LESLKAGGVT PKFVIIDDGW
     QSVGMDETSV EFNADNAANF ANRLTHIKEN HKFQKDGKEG HRVDDPSLSL GHVITDIKSN
     NSLKYVYVWH AITGYWGGVK PGVSGMEHYE SKVAYPVSSP GVMSSENCGC LESITKNGLG
     LVNPEKVFSF YNDLHSYLAS VGVDGVKVDV QNILETLGAG HGGRVKLAKK YHQALEASIS
     RNFPDNGIIS CMSHNTDGLY SAKKTAVIRA SDDFWPRDPA SHTIHIASVA YNTLFLGEFM
     QPDWDMFHSL HPMAEYHAAA RAVGGCAIYV SDKPGQHDFN LLRKLVLRDG SILRAKLPGR
     PTSDCFFSDP VRDNKSLLKI WNLNEFTGVI GVFNCQGAGW CKNEKRYLIH DQEPGTISGC
     VRTNDVHYLH KVAAFEWTGD SIVYSHLRGE LVYLPKDTSL PVTLMPREYE VFTVVPVKEF
     SDGSKFAPVG LMEMFNSGGA IVSLRYDDEG TKFVVRMKLR GSGLVGVYSS VRRPRSVTVD
     SDDVEYRYEP ESGLVTFTLG VPEKELYLWD VVIQ
//