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Protein

Probable galactinol--sucrose galactosyltransferase 1

Gene

RFS1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Transglycosidase operating by a ping-pong reaction mechanism. Involved in the synthesis of raffinose, a major soluble carbohydrate in seeds, roots and tubers (By similarity).By similarity

Catalytic activityi

Alpha-D-galactosyl-(1->3)-1D-myo-inositol + sucrose = myo-inositol + raffinose.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processCarbohydrate metabolism

Enzyme and pathway databases

BioCyciARA:AT1G55740-MONOMER
BRENDAi2.4.1.82 399

Protein family/group databases

CAZyiGH36 Glycoside Hydrolase Family 36

Names & Taxonomyi

Protein namesi
Recommended name:
Probable galactinol--sucrose galactosyltransferase 1 (EC:2.4.1.82)
Alternative name(s):
Protein SEED IMBIBITION 1
Raffinose synthase 1
Gene namesi
Name:RFS1
Synonyms:RS1, SIP1
Ordered Locus Names:At1g55740
ORF Names:F20N2.12, F20N2.14
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G55740
TAIRilocus:2020452 AT1G55740

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002701571 – 754Probable galactinol--sucrose galactosyltransferase 1Add BLAST754

Proteomic databases

PaxDbiQ84VX0
PRIDEiQ84VX0

Expressioni

Inductioni

Not induced by oxidative stress.1 Publication

Gene expression databases

ExpressionAtlasiQ84VX0 baseline and differential
GenevisibleiQ84VX0 AT

Interactioni

Protein-protein interaction databases

BioGridi27248, 1 interactor
IntActiQ84VX0, 1 interactor
STRINGi3702.AT1G55740.1

Structurei

3D structure databases

ProteinModelPortaliQ84VX0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolases 36 family.Curated

Phylogenomic databases

eggNOGiENOG410IH7P Eukaryota
ENOG410YBU9 LUCA
HOGENOMiHOG000237551
InParanoidiQ84VX0
KOiK06617
OMAiSGMEHYE
OrthoDBiEOG093603F4
PhylomeDBiQ84VX0

Family and domain databases

Gene3Di3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR017853 Glycoside_hydrolase_SF
IPR008811 Glycosyl_hydrolases_36
PANTHERiPTHR31268 PTHR31268, 1 hit
PfamiView protein in Pfam
PF05691 Raffinose_syn, 1 hit
SUPFAMiSSF51445 SSF51445, 3 hits

Sequencei

Sequence statusi: Complete.

Q84VX0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTVGAGISVT DSDLVVLGHR VLHGVPENVL VTPASGNALI DGAFIGVTSD
60 70 80 90 100
QTGSHRVFSL GKLEDLRFMC VFRFKLWWMT QRMGTNGKEI PCETQFLIVE
110 120 130 140 150
ANQGSDLGGR DQSSSYVVFL PILEGDFRAV LQGNEANELE ICLESGDPTV
160 170 180 190 200
DQFEGSHLVF VAAGSDPFDV ITKAVKAVEQ HLQTFSHRER KKMPDMLNWF
210 220 230 240 250
GWCTWDAFYT NVTAKDVKQG LESLKAGGVT PKFVIIDDGW QSVGMDETSV
260 270 280 290 300
EFNADNAANF ANRLTHIKEN HKFQKDGKEG HRVDDPSLSL GHVITDIKSN
310 320 330 340 350
NSLKYVYVWH AITGYWGGVK PGVSGMEHYE SKVAYPVSSP GVMSSENCGC
360 370 380 390 400
LESITKNGLG LVNPEKVFSF YNDLHSYLAS VGVDGVKVDV QNILETLGAG
410 420 430 440 450
HGGRVKLAKK YHQALEASIS RNFPDNGIIS CMSHNTDGLY SAKKTAVIRA
460 470 480 490 500
SDDFWPRDPA SHTIHIASVA YNTLFLGEFM QPDWDMFHSL HPMAEYHAAA
510 520 530 540 550
RAVGGCAIYV SDKPGQHDFN LLRKLVLRDG SILRAKLPGR PTSDCFFSDP
560 570 580 590 600
VRDNKSLLKI WNLNEFTGVI GVFNCQGAGW CKNEKRYLIH DQEPGTISGC
610 620 630 640 650
VRTNDVHYLH KVAAFEWTGD SIVYSHLRGE LVYLPKDTSL PVTLMPREYE
660 670 680 690 700
VFTVVPVKEF SDGSKFAPVG LMEMFNSGGA IVSLRYDDEG TKFVVRMKLR
710 720 730 740 750
GSGLVGVYSS VRRPRSVTVD SDDVEYRYEP ESGLVTFTLG VPEKELYLWD

VVIQ
Length:754
Mass (Da):83,477
Last modified:June 1, 2003 - v1
Checksum:iC57DB5076DADA8AD
GO

Sequence cautioni

The sequence AAF79504 differs from that shown. Reason: Erroneous gene model prediction. The predicted gene has been split into 2 genes: At1g55730 and At1g55740.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC002328 Genomic DNA Translation: AAF79504.1 Sequence problems.
CP002684 Genomic DNA Translation: AEE33292.1
BT004640 mRNA Translation: AAO42886.1
AK227977 mRNA Translation: BAE99943.1
PIRiC96599
RefSeqiNP_175970.1, NM_104450.4
UniGeneiAt.47524
At.67212

Genome annotation databases

EnsemblPlantsiAT1G55740.1; AT1G55740.1; AT1G55740
GeneIDi842023
GrameneiAT1G55740.1; AT1G55740.1; AT1G55740
KEGGiath:AT1G55740

Similar proteinsi

Entry informationi

Entry nameiRFS1_ARATH
AccessioniPrimary (citable) accession number: Q84VX0
Secondary accession number(s): Q9LFZ7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: June 1, 2003
Last modified: May 23, 2018
This is version 90 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families
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