Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q84VX0 (RFS1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable galactinol--sucrose galactosyltransferase 1

EC=2.4.1.82
Alternative name(s):
Protein SEED IMBIBITION 1
Raffinose synthase 1
Gene names
Name:RFS1
Synonyms:RS1, SIP1
Ordered Locus Names:At1g55740
ORF Names:F20N2.12, F20N2.14
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length754 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Transglycosidase operating by a ping-pong reaction mechanism. Involved in the synthesis of raffinose, a major soluble carbohydrate in seeds, roots and tubers By similarity.

Catalytic activity

Alpha-D-galactosyl-(1->3)-1D-myo-inositol + sucrose = myo-inositol + raffinose.

Induction

Not induced by oxidative stress. Ref.5

Sequence similarities

Belongs to the glycosyl hydrolases 36 family.

Sequence caution

The sequence AAF79504.1 differs from that shown. Reason: Erroneous gene model prediction. The predicted gene has been split into 2 genes: At1g55730 and At1g55740.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
   Molecular functionGlycosyltransferase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functiongalactinol-sucrose galactosyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 754754Probable galactinol--sucrose galactosyltransferase 1
PRO_0000270157

Sequences

Sequence LengthMass (Da)Tools
Q84VX0 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: C57DB5076DADA8AD

FASTA75483,477
        10         20         30         40         50         60 
MTVGAGISVT DSDLVVLGHR VLHGVPENVL VTPASGNALI DGAFIGVTSD QTGSHRVFSL 

        70         80         90        100        110        120 
GKLEDLRFMC VFRFKLWWMT QRMGTNGKEI PCETQFLIVE ANQGSDLGGR DQSSSYVVFL 

       130        140        150        160        170        180 
PILEGDFRAV LQGNEANELE ICLESGDPTV DQFEGSHLVF VAAGSDPFDV ITKAVKAVEQ 

       190        200        210        220        230        240 
HLQTFSHRER KKMPDMLNWF GWCTWDAFYT NVTAKDVKQG LESLKAGGVT PKFVIIDDGW 

       250        260        270        280        290        300 
QSVGMDETSV EFNADNAANF ANRLTHIKEN HKFQKDGKEG HRVDDPSLSL GHVITDIKSN 

       310        320        330        340        350        360 
NSLKYVYVWH AITGYWGGVK PGVSGMEHYE SKVAYPVSSP GVMSSENCGC LESITKNGLG 

       370        380        390        400        410        420 
LVNPEKVFSF YNDLHSYLAS VGVDGVKVDV QNILETLGAG HGGRVKLAKK YHQALEASIS 

       430        440        450        460        470        480 
RNFPDNGIIS CMSHNTDGLY SAKKTAVIRA SDDFWPRDPA SHTIHIASVA YNTLFLGEFM 

       490        500        510        520        530        540 
QPDWDMFHSL HPMAEYHAAA RAVGGCAIYV SDKPGQHDFN LLRKLVLRDG SILRAKLPGR 

       550        560        570        580        590        600 
PTSDCFFSDP VRDNKSLLKI WNLNEFTGVI GVFNCQGAGW CKNEKRYLIH DQEPGTISGC 

       610        620        630        640        650        660 
VRTNDVHYLH KVAAFEWTGD SIVYSHLRGE LVYLPKDTSL PVTLMPREYE VFTVVPVKEF 

       670        680        690        700        710        720 
SDGSKFAPVG LMEMFNSGGA IVSLRYDDEG TKFVVRMKLR GSGLVGVYSS VRRPRSVTVD 

       730        740        750 
SDDVEYRYEP ESGLVTFTLG VPEKELYLWD VVIQ 

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. expand/collapse author list , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Galactinol and raffinose constitute a novel function to protect plants from oxidative damage."
Nishizawa A., Yabuta Y., Shigeoka S.
Plant Physiol. 147:1251-1263(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION BY OXIDATIVE STRESS, GENE FAMILY, NOMENCLATURE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC002328 Genomic DNA. Translation: AAF79504.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE33292.1.
BT004640 mRNA. Translation: AAO42886.1.
AK227977 mRNA. Translation: BAE99943.1.
PIRC96599.
RefSeqNP_175970.1. NM_104450.3.
UniGeneAt.47524.
At.67212.

3D structure databases

ProteinModelPortalQ84VX0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid27248. 1 interaction.
IntActQ84VX0. 1 interaction.
STRING3702.AT1G55740.1-P.

Protein family/group databases

CAZyGH36. Glycoside Hydrolase Family 36.

Proteomic databases

PaxDbQ84VX0.
PRIDEQ84VX0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G55740.1; AT1G55740.1; AT1G55740.
GeneID842023.
KEGGath:AT1G55740.

Organism-specific databases

TAIRAT1G55740.

Phylogenomic databases

eggNOGNOG06986.
HOGENOMHOG000237551.
InParanoidQ84VX0.
KOK06617.
OMATQFLIVE.
PhylomeDBQ84VX0.
ProtClustDBPLN02355.

Enzyme and pathway databases

BioCycARA:AT1G55740-MONOMER.

Gene expression databases

GenevestigatorQ84VX0.

Family and domain databases

Gene3D3.20.20.70. 2 hits.
InterProIPR013785. Aldolase_TIM.
IPR017853. Glycoside_hydrolase_SF.
IPR008811. Glycosyl_hydrolases_36.
[Graphical view]
PfamPF05691. Raffinose_syn. 1 hit.
[Graphical view]
SUPFAMSSF51445. SSF51445. 3 hits.
ProtoNetSearch...

Entry information

Entry nameRFS1_ARATH
AccessionPrimary (citable) accession number: Q84VX0
Secondary accession number(s): Q9LFZ7
Entry history
Integrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: June 1, 2003
Last modified: February 19, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names