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Q84VX0

- RFS1_ARATH

UniProt

Q84VX0 - RFS1_ARATH

Protein

Probable galactinol--sucrose galactosyltransferase 1

Gene

RFS1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 71 (01 Oct 2014)
      Sequence version 1 (01 Jun 2003)
      Previous versions | rss
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    Functioni

    Transglycosidase operating by a ping-pong reaction mechanism. Involved in the synthesis of raffinose, a major soluble carbohydrate in seeds, roots and tubers By similarity.By similarity

    Catalytic activityi

    Alpha-D-galactosyl-(1->3)-1D-myo-inositol + sucrose = myo-inositol + raffinose.

    GO - Molecular functioni

    1. galactinol-sucrose galactosyltransferase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Carbohydrate metabolism

    Enzyme and pathway databases

    BioCyciARA:AT1G55740-MONOMER.

    Protein family/group databases

    CAZyiGH36. Glycoside Hydrolase Family 36.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable galactinol--sucrose galactosyltransferase 1 (EC:2.4.1.82)
    Alternative name(s):
    Protein SEED IMBIBITION 1
    Raffinose synthase 1
    Gene namesi
    Name:RFS1
    Synonyms:RS1, SIP1
    Ordered Locus Names:At1g55740
    ORF Names:F20N2.12, F20N2.14
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G55740.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 754754Probable galactinol--sucrose galactosyltransferase 1PRO_0000270157Add
    BLAST

    Proteomic databases

    PaxDbiQ84VX0.
    PRIDEiQ84VX0.

    Expressioni

    Inductioni

    Not induced by oxidative stress.1 Publication

    Gene expression databases

    GenevestigatoriQ84VX0.

    Interactioni

    Protein-protein interaction databases

    BioGridi27248. 1 interaction.
    IntActiQ84VX0. 1 interaction.
    STRINGi3702.AT1G55740.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ84VX0.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolases 36 family.Curated

    Phylogenomic databases

    eggNOGiNOG06986.
    HOGENOMiHOG000237551.
    InParanoidiQ84VX0.
    KOiK06617.
    OMAiTQFLIVE.
    PhylomeDBiQ84VX0.

    Family and domain databases

    Gene3Di3.20.20.70. 2 hits.
    InterProiIPR013785. Aldolase_TIM.
    IPR017853. Glycoside_hydrolase_SF.
    IPR008811. Glycosyl_hydrolases_36.
    [Graphical view]
    PfamiPF05691. Raffinose_syn. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 3 hits.

    Sequencei

    Sequence statusi: Complete.

    Q84VX0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTVGAGISVT DSDLVVLGHR VLHGVPENVL VTPASGNALI DGAFIGVTSD    50
    QTGSHRVFSL GKLEDLRFMC VFRFKLWWMT QRMGTNGKEI PCETQFLIVE 100
    ANQGSDLGGR DQSSSYVVFL PILEGDFRAV LQGNEANELE ICLESGDPTV 150
    DQFEGSHLVF VAAGSDPFDV ITKAVKAVEQ HLQTFSHRER KKMPDMLNWF 200
    GWCTWDAFYT NVTAKDVKQG LESLKAGGVT PKFVIIDDGW QSVGMDETSV 250
    EFNADNAANF ANRLTHIKEN HKFQKDGKEG HRVDDPSLSL GHVITDIKSN 300
    NSLKYVYVWH AITGYWGGVK PGVSGMEHYE SKVAYPVSSP GVMSSENCGC 350
    LESITKNGLG LVNPEKVFSF YNDLHSYLAS VGVDGVKVDV QNILETLGAG 400
    HGGRVKLAKK YHQALEASIS RNFPDNGIIS CMSHNTDGLY SAKKTAVIRA 450
    SDDFWPRDPA SHTIHIASVA YNTLFLGEFM QPDWDMFHSL HPMAEYHAAA 500
    RAVGGCAIYV SDKPGQHDFN LLRKLVLRDG SILRAKLPGR PTSDCFFSDP 550
    VRDNKSLLKI WNLNEFTGVI GVFNCQGAGW CKNEKRYLIH DQEPGTISGC 600
    VRTNDVHYLH KVAAFEWTGD SIVYSHLRGE LVYLPKDTSL PVTLMPREYE 650
    VFTVVPVKEF SDGSKFAPVG LMEMFNSGGA IVSLRYDDEG TKFVVRMKLR 700
    GSGLVGVYSS VRRPRSVTVD SDDVEYRYEP ESGLVTFTLG VPEKELYLWD 750
    VVIQ 754
    Length:754
    Mass (Da):83,477
    Last modified:June 1, 2003 - v1
    Checksum:iC57DB5076DADA8AD
    GO

    Sequence cautioni

    The sequence AAF79504.1 differs from that shown. Reason: Erroneous gene model prediction. The predicted gene has been split into 2 genes: At1g55730 and At1g55740.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC002328 Genomic DNA. Translation: AAF79504.1. Sequence problems.
    CP002684 Genomic DNA. Translation: AEE33292.1.
    BT004640 mRNA. Translation: AAO42886.1.
    AK227977 mRNA. Translation: BAE99943.1.
    PIRiC96599.
    RefSeqiNP_175970.1. NM_104450.3.
    UniGeneiAt.47524.
    At.67212.

    Genome annotation databases

    EnsemblPlantsiAT1G55740.1; AT1G55740.1; AT1G55740.
    GeneIDi842023.
    KEGGiath:AT1G55740.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC002328 Genomic DNA. Translation: AAF79504.1 . Sequence problems.
    CP002684 Genomic DNA. Translation: AEE33292.1 .
    BT004640 mRNA. Translation: AAO42886.1 .
    AK227977 mRNA. Translation: BAE99943.1 .
    PIRi C96599.
    RefSeqi NP_175970.1. NM_104450.3.
    UniGenei At.47524.
    At.67212.

    3D structure databases

    ProteinModelPortali Q84VX0.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 27248. 1 interaction.
    IntActi Q84VX0. 1 interaction.
    STRINGi 3702.AT1G55740.1-P.

    Protein family/group databases

    CAZyi GH36. Glycoside Hydrolase Family 36.

    Proteomic databases

    PaxDbi Q84VX0.
    PRIDEi Q84VX0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G55740.1 ; AT1G55740.1 ; AT1G55740 .
    GeneIDi 842023.
    KEGGi ath:AT1G55740.

    Organism-specific databases

    TAIRi AT1G55740.

    Phylogenomic databases

    eggNOGi NOG06986.
    HOGENOMi HOG000237551.
    InParanoidi Q84VX0.
    KOi K06617.
    OMAi TQFLIVE.
    PhylomeDBi Q84VX0.

    Enzyme and pathway databases

    BioCyci ARA:AT1G55740-MONOMER.

    Gene expression databases

    Genevestigatori Q84VX0.

    Family and domain databases

    Gene3Di 3.20.20.70. 2 hits.
    InterProi IPR013785. Aldolase_TIM.
    IPR017853. Glycoside_hydrolase_SF.
    IPR008811. Glycosyl_hydrolases_36.
    [Graphical view ]
    Pfami PF05691. Raffinose_syn. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 3 hits.
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
      Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
      , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
      Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Galactinol and raffinose constitute a novel function to protect plants from oxidative damage."
      Nishizawa A., Yabuta Y., Shigeoka S.
      Plant Physiol. 147:1251-1263(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION BY OXIDATIVE STRESS, GENE FAMILY, NOMENCLATURE.

    Entry informationi

    Entry nameiRFS1_ARATH
    AccessioniPrimary (citable) accession number: Q84VX0
    Secondary accession number(s): Q9LFZ7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 9, 2007
    Last sequence update: June 1, 2003
    Last modified: October 1, 2014
    This is version 71 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3