Q84VX0 (RFS1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable galactinol--sucrose galactosyltransferase 1
EC=2.4.1.82

Alternative name(s):
Protein SEED IMBIBITION 1
Raffinose synthase 1

Gene names

Name:	RFS1
Synonyms:	RS1, SIP1
Ordered Locus Names:	At1g55740
ORF Names:	F20N2.12, F20N2.14

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	754 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Transglycosidase operating by a ping-pong reaction mechanism. Involved in the synthesis of raffinose, a major soluble carbohydrate in seeds, roots and tubers By similarity.
Catalytic activity	Alpha-D-galactosyl-(1->3)-1D-myo-inositol + sucrose = myo-inositol + raffinose.
Induction	Not induced by oxidative stress. Ref.5
Sequence similarities	Belongs to the glycosyl hydrolases 36 family.
Sequence caution	The sequence AAF79504.1 differs from that shown. Reason: Erroneous gene model prediction. The predicted gene has been split into 2 genes: At1g55730 and At1g55740.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	galactinol-sucrose galactosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 754	754	Probable galactinol--sucrose galactosyltransferase 1		PRO_0000270157

Sequences

	Sequence		Length	Mass (Da)
	Q84VX0 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: C57DB5076DADA8AD	FASTA	754	83,477
	10 20 30 40 50 60 MTVGAGISVT DSDLVVLGHR VLHGVPENVL VTPASGNALI DGAFIGVTSD QTGSHRVFSL 70 80 90 100 110 120 GKLEDLRFMC VFRFKLWWMT QRMGTNGKEI PCETQFLIVE ANQGSDLGGR DQSSSYVVFL 130 140 150 160 170 180 PILEGDFRAV LQGNEANELE ICLESGDPTV DQFEGSHLVF VAAGSDPFDV ITKAVKAVEQ 190 200 210 220 230 240 HLQTFSHRER KKMPDMLNWF GWCTWDAFYT NVTAKDVKQG LESLKAGGVT PKFVIIDDGW 250 260 270 280 290 300 QSVGMDETSV EFNADNAANF ANRLTHIKEN HKFQKDGKEG HRVDDPSLSL GHVITDIKSN 310 320 330 340 350 360 NSLKYVYVWH AITGYWGGVK PGVSGMEHYE SKVAYPVSSP GVMSSENCGC LESITKNGLG 370 380 390 400 410 420 LVNPEKVFSF YNDLHSYLAS VGVDGVKVDV QNILETLGAG HGGRVKLAKK YHQALEASIS 430 440 450 460 470 480 RNFPDNGIIS CMSHNTDGLY SAKKTAVIRA SDDFWPRDPA SHTIHIASVA YNTLFLGEFM 490 500 510 520 530 540 QPDWDMFHSL HPMAEYHAAA RAVGGCAIYV SDKPGQHDFN LLRKLVLRDG SILRAKLPGR 550 560 570 580 590 600 PTSDCFFSDP VRDNKSLLKI WNLNEFTGVI GVFNCQGAGW CKNEKRYLIH DQEPGTISGC 610 620 630 640 650 660 VRTNDVHYLH KVAAFEWTGD SIVYSHLRGE LVYLPKDTSL PVTLMPREYE VFTVVPVKEF 670 680 690 700 710 720 SDGSKFAPVG LMEMFNSGGA IVSLRYDDEG TKFVVRMKLR GSGLVGVYSS VRRPRSVTVD 730 740 750 SDDVEYRYEP ESGLVTFTLG VPEKELYLWD VVIQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Galactinol and raffinose constitute a novel function to protect plants from oxidative damage." Nishizawa A., Yabuta Y., Shigeoka S. Plant Physiol. 147:1251-1263(2008) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION BY OXIDATIVE STRESS, GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC002328 Genomic DNA. Translation: AAF79504.1. Sequence problems. CP002684 Genomic DNA. Translation: AEE33292.1. BT004640 mRNA. Translation: AAO42886.1. AK227977 mRNA. Translation: BAE99943.1.
IPI	IPI00543579.
PIR	C96599.
RefSeq	NP_175970.1. NM_104450.3.
UniGene	At.47524. At.67212.
3D structure databases
ProteinModelPortal	Q84VX0.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q84VX0. 1 interaction.
STRING	3702.AT1G55740.1-P.
Protein family/group databases
CAZy	GH36. Glycoside Hydrolase Family 36.
Proteomic databases
PaxDb	Q84VX0.
PRIDE	Q84VX0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G55740.1; AT1G55740.1; AT1G55740.
GeneID	842023.
KEGG	ath:AT1G55740.
Organism-specific databases
TAIR	At1g55740.
Phylogenomic databases
eggNOG	NOG06986.
HOGENOM	HOG000237551.
InParanoid	Q84VX0.
KO	K06617.
OMA	LTHIKEN.
PhylomeDB	Q84VX0.
ProtClustDB	PLN02355.
Gene expression databases
Genevestigator	Q84VX0.
Family and domain databases
Gene3D	3.20.20.70. 2 hits.
InterPro	IPR013785. Aldolase_TIM. IPR017853. Glycoside_hydrolase_SF. IPR008811. Raffinose_synthase. [Graphical view]
Pfam	PF05691. Raffinose_syn. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RFS1_ARATH

Accession

Primary (citable) accession number: Q84VX0
Secondary accession number(s): Q9LFZ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 9, 2007
Last sequence update:	June 1, 2003
Last modified:	May 1, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program