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Q84VW9 (CAPP3_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoenolpyruvate carboxylase 3

Short name=AtPPC3
Short name=PEPC 3
Short name=PEPCase 3
EC=4.1.1.31
Gene names
Name:PPC3
Synonyms:PEPC, PPC
Ordered Locus Names:At3g14940
ORF Names:K15M2.8
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length968 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Through the carboxylation of phosphoenolpyruvate (PEP) it forms oxaloacetate, a four-carbon dicarboxylic acid source for the tricarboxylic acid cycle. HAMAP-Rule MF_00595

Catalytic activity

Phosphate + oxaloacetate = H2O + phosphoenolpyruvate + HCO3-. HAMAP-Rule MF_00595

Cofactor

Magnesium By similarity. HAMAP-Rule MF_00595

Enzyme regulation

By light-reversible phosphorylation By similarity. HAMAP-Rule MF_00595

Subunit structure

Homotetramer By similarity. HAMAP-Rule MF_00595

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00595.

Tissue specificity

Expressed in roots and siliques, and to a lower extent in stems, leaves and flowers. Ref.7

Sequence similarities

Belongs to the PEPCase type 1 family.

Ontologies

Keywords
   Biological processCarbon dioxide fixation
Photosynthesis
   Cellular componentCytoplasm
   LigandMagnesium
   Molecular functionLyase
   PTMPhosphoprotein
   Technical termAllosteric enzyme
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcarbon fixation

Inferred from electronic annotation. Source: UniProtKB-KW

photosynthesis

Inferred from electronic annotation. Source: UniProtKB-KW

tricarboxylic acid cycle

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytosol

Inferred from direct assay PubMed 18433157. Source: TAIR

   Molecular_functionphosphoenolpyruvate carboxylase activity

Inferred from direct assay PubMed 17894783. Source: TAIR

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 968968Phosphoenolpyruvate carboxylase 3 HAMAP-Rule MF_00595
PRO_0000166659

Sites

Active site1731 By similarity
Active site6031 By similarity

Amino acid modifications

Modified residue111Phosphoserine By similarity
Modified residue7051Phosphoserine By similarity

Experimental info

Sequence conflict2451H → R in AAO42888. Ref.5
Sequence conflict2451H → R in BAE99551. Ref.6

Sequences

Sequence LengthMass (Da)Tools
Q84VW9 [UniParc].

Last modified August 30, 2005. Version 2.
Checksum: 1CECE8B8981F63F0

FASTA968110,160
        10         20         30         40         50         60 
MAGRNIEKMA SIDAQLRQLV PAKVSEDDKL VEYDALLLDR FLDILQDLHG EDLRETVQEL 

        70         80         90        100        110        120 
YELSAEYEGK REPSKLEELG SVLTSLDPGD SIVISKAFSH MLNLANLAEE VQIAHRRRIK 

       130        140        150        160        170        180 
KLKKGDFVDE SSATTESDIE ETFKRLVSDL GKSPEEIFDA LKNQTVDLVL TAHPTQSVRR 

       190        200        210        220        230        240 
SLLQKHGRIR DCLAQLYAKD ITPDDKQELD ESLQREIQAA FRTDEIRRTP PTPQDEMRAG 

       250        260        270        280        290        300 
MSYFHETIWK GVPKFLRRVD TALKNIGIDE RVPYNAPLIQ FSSWMGGDRD GNPRVTPEVT 

       310        320        330        340        350        360 
RDVCLLARMM AANLYYNQIE NLMFELSMWR CTDEFRVRAD ELHRNSRKDA AKHYIEFWKT 

       370        380        390        400        410        420 
IPPTEPYRVI LGDVRDKLYH TRERSRQLLS NGISDIPEEA TFTNVEQFLE PLELCYRSLC 

       430        440        450        460        470        480 
SCGDSPIADG SLLDFLRQVS TFGLSLVRLD IRQESERHTD VLDAITKHLD IGSSYRDWSE 

       490        500        510        520        530        540 
EGRQEWLLAE LSGKRPLFGP DLPKTEEISD VLDTFKVISE LPSDCFGAYI ISMATSPSDV 

       550        560        570        580        590        600 
LAVELLQREC HVKNPLRVVP LFEKLADLEA APAAVARLFS IDWYKNRING KQEVMIGYSD 

       610        620        630        640        650        660 
SGKDAGRLSA AWELYKAQEE LVKVAKKYGV KLTMFHGRGG TVGRGGGPTH LAILSQPPDT 

       670        680        690        700        710        720 
VNGSLRVTVQ GEVIEQSFGE AHLCFRTLQR FTAATLEHGM NPPISPKPEW RALLDEMAVV 

       730        740        750        760        770        780 
ATEEYRSVVF QEPRFVEYFR LATPELEYGR MNIGSRPSKR KPSGGIESLR AIPWIFAWTQ 

       790        800        810        820        830        840 
TRFHLPVWLG FGAAFRYAIK KDVRNLHMLQ DMYKQWPFFR VTIDLIEMVF AKGDPGIAAL 

       850        860        870        880        890        900 
YDKLLVSEDL WAFGEKLRAN FDETKNLVLQ TAGHKDLLEG DPYLKQRLRL RDSYITTLNV 

       910        920        930        940        950        960 
CQAYTLKRIR DANYNVTLRP HISKEIMQSS KSAQELVKLN PTSEYAPGLE DTLILTMKGI 


AAGLQNTG 

« Hide

References

« Hide 'large scale' references
[1]"Arabidopsis thaliana phosphoenolpyruvate carboxylase full-length cDNA."
Paterson K.M., Nimmo H.G.
Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Columbia.
[2]"Genomic structure of PEPC in Arabidopsis thaliana."
Hartung F.
Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: cv. Columbia.
Tissue: Seedling.
[3]"Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones."
Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.
DNA Res. 7:217-221(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[4]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[5]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[6]"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. expand/collapse author list , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[7]"Identification and expression analysis of a gene encoding a bacterial-type phosphoenolpyruvate carboxylase from Arabidopsis and rice."
Sanchez R., Cejudo F.J.
Plant Physiol. 132:949-957(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, NOMENCLATURE.
Strain: cv. Columbia.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF071788 mRNA. Translation: AAC24594.1.
AJ131710 Genomic DNA. Translation: CAA10486.1.
AP000370 Genomic DNA. Translation: BAA97057.1.
CP002686 Genomic DNA. Translation: AEE75592.1.
BT004642 mRNA. Translation: AAO42888.1.
AK227556 mRNA. Translation: BAE99551.1.
PIRT52186.
RefSeqNP_188112.1. NM_112356.3.
UniGeneAt.24126.
At.74839.

3D structure databases

ProteinModelPortalQ84VW9.
SMRQ84VW9. Positions 6-968.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid6057. 1 interaction.
MINTMINT-8060497.

Proteomic databases

PaxDbQ84VW9.
PRIDEQ84VW9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT3G14940.1; AT3G14940.1; AT3G14940.
GeneID820723.
KEGGath:AT3G14940.

Organism-specific databases

GeneFarm5062. 479.
TAIRAT3G14940.

Phylogenomic databases

eggNOGCOG2352.
HOGENOMHOG000238648.
InParanoidQ84VW9.
KOK01595.
OMAWYGAGSA.
PhylomeDBQ84VW9.

Enzyme and pathway databases

BioCycARA:AT3G14940-MONOMER.
MetaCyc:AT3G14940-MONOMER.
BRENDA4.1.1.31. 399.

Gene expression databases

GenevestigatorQ84VW9.

Family and domain databases

HAMAPMF_00595. PEPcase_type1.
InterProIPR021135. PEP_COase.
IPR018129. PEP_COase_AS.
IPR022805. PEP_COase_bac/pln-type.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
PfamPF00311. PEPcase. 1 hit.
[Graphical view]
PRINTSPR00150. PEPCARBXLASE.
SUPFAMSSF51621. SSF51621. 2 hits.
PROSITEPS00781. PEPCASE_1. 1 hit.
PS00393. PEPCASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCAPP3_ARATH
AccessionPrimary (citable) accession number: Q84VW9
Secondary accession number(s): O81357, Q0WTJ7
Entry history
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: August 30, 2005
Last modified: May 14, 2014
This is version 82 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names