Q84VW9 (CAPP3_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoenolpyruvate carboxylase 3
Short name=AtPPC3
Short name=PEPC 3
Short name=PEPCase 3
EC=4.1.1.31

Gene names

Name:	PPC3
Synonyms:	PEPC, PPC
Ordered Locus Names:	At3g14940
ORF Names:	K15M2.8

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	968 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Through the carboxylation of phosphoenolpyruvate (PEP) it forms oxaloacetate, a four-carbon dicarboxylic acid source for the tricarboxylic acid cycle.
Catalytic activity	Phosphate + oxaloacetate = H2O + phosphoenolpyruvate + HCO3-.
Cofactor	Magnesium By similarity.
Enzyme regulation	By light-reversible phosphorylation By similarity.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Tissue specificity	Expressed in roots and siliques, and to a lower extent in stems, leaves and flowers. Ref.7
Sequence similarities	Belongs to the PEPCase type 1 family.

Ontologies

Keywords
Biological process	Carbon dioxide fixation Photosynthesis
Cellular component	Cytoplasm
Ligand	Magnesium
Molecular function	Lyase
PTM	Phosphoprotein
Technical term	Allosteric enzyme Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	carbon fixation Inferred from electronic annotation. Source: UniProtKB-KW photosynthesis Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: InterPro
Cellular_component	cytosol Inferred from direct assay PubMed 18433157. Source: TAIR
Molecular_function	phosphoenolpyruvate carboxylase activity Inferred from direct assay PubMed 17894783. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 968	968	Phosphoenolpyruvate carboxylase 3		PRO_0000166659
Sites
	Active site	173	1	By similarity
	Active site	603	1	By similarity
Amino acid modifications
	Modified residue	11	1	Phosphoserine By similarity
Experimental info
	Sequence conflict	245	1	H → R in AAO42888. Ref.5
	Sequence conflict	245	1	H → R in BAE99551. Ref.6

Sequences

	Sequence		Length	Mass (Da)
	Q84VW9 [UniParc]. Last modified August 30, 2005. Version 2. Checksum: 1CECE8B8981F63F0	FASTA	968	110,160
	10 20 30 40 50 60 MAGRNIEKMA SIDAQLRQLV PAKVSEDDKL VEYDALLLDR FLDILQDLHG EDLRETVQEL 70 80 90 100 110 120 YELSAEYEGK REPSKLEELG SVLTSLDPGD SIVISKAFSH MLNLANLAEE VQIAHRRRIK 130 140 150 160 170 180 KLKKGDFVDE SSATTESDIE ETFKRLVSDL GKSPEEIFDA LKNQTVDLVL TAHPTQSVRR 190 200 210 220 230 240 SLLQKHGRIR DCLAQLYAKD ITPDDKQELD ESLQREIQAA FRTDEIRRTP PTPQDEMRAG 250 260 270 280 290 300 MSYFHETIWK GVPKFLRRVD TALKNIGIDE RVPYNAPLIQ FSSWMGGDRD GNPRVTPEVT 310 320 330 340 350 360 RDVCLLARMM AANLYYNQIE NLMFELSMWR CTDEFRVRAD ELHRNSRKDA AKHYIEFWKT 370 380 390 400 410 420 IPPTEPYRVI LGDVRDKLYH TRERSRQLLS NGISDIPEEA TFTNVEQFLE PLELCYRSLC 430 440 450 460 470 480 SCGDSPIADG SLLDFLRQVS TFGLSLVRLD IRQESERHTD VLDAITKHLD IGSSYRDWSE 490 500 510 520 530 540 EGRQEWLLAE LSGKRPLFGP DLPKTEEISD VLDTFKVISE LPSDCFGAYI ISMATSPSDV 550 560 570 580 590 600 LAVELLQREC HVKNPLRVVP LFEKLADLEA APAAVARLFS IDWYKNRING KQEVMIGYSD 610 620 630 640 650 660 SGKDAGRLSA AWELYKAQEE LVKVAKKYGV KLTMFHGRGG TVGRGGGPTH LAILSQPPDT 670 680 690 700 710 720 VNGSLRVTVQ GEVIEQSFGE AHLCFRTLQR FTAATLEHGM NPPISPKPEW RALLDEMAVV 730 740 750 760 770 780 ATEEYRSVVF QEPRFVEYFR LATPELEYGR MNIGSRPSKR KPSGGIESLR AIPWIFAWTQ 790 800 810 820 830 840 TRFHLPVWLG FGAAFRYAIK KDVRNLHMLQ DMYKQWPFFR VTIDLIEMVF AKGDPGIAAL 850 860 870 880 890 900 YDKLLVSEDL WAFGEKLRAN FDETKNLVLQ TAGHKDLLEG DPYLKQRLRL RDSYITTLNV 910 920 930 940 950 960 CQAYTLKRIR DANYNVTLRP HISKEIMQSS KSAQELVKLN PTSEYAPGLE DTLILTMKGI AAGLQNTG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Arabidopsis thaliana phosphoenolpyruvate carboxylase full-length cDNA." Paterson K.M., Nimmo H.G. Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia.
[2]	"Genomic structure of PEPC in Arabidopsis thaliana." Hartung F. Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Columbia. Tissue: Seedling.
[3]	"Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones." Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S. DNA Res. 7:217-221(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[5]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[6]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[7]	"Identification and expression analysis of a gene encoding a bacterial-type phosphoenolpyruvate carboxylase from Arabidopsis and rice." Sanchez R., Cejudo F.J. Plant Physiol. 132:949-957(2003) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY, NOMENCLATURE. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF071788 mRNA. Translation: AAC24594.1. AJ131710 Genomic DNA. Translation: CAA10486.1. AP000370 Genomic DNA. Translation: BAA97057.1. CP002686 Genomic DNA. Translation: AEE75592.1. BT004642 mRNA. Translation: AAO42888.1. AK227556 mRNA. Translation: BAE99551.1.
IPI	IPI00522131.
PIR	T52186.
RefSeq	NP_188112.1. NM_112356.3.
UniGene	At.24126. At.74839.
3D structure databases
ProteinModelPortal	Q84VW9.
SMR	Q84VW9. Positions 31-968.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-8060497.
Proteomic databases
PaxDb	Q84VW9.
PRIDE	Q84VW9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT3G14940.1; AT3G14940.1; AT3G14940.
GeneID	820723.
KEGG	ath:AT3G14940.
Organism-specific databases
GeneFarm	5062. 479.
TAIR	At3g14940.
Phylogenomic databases
eggNOG	COG2352.
HOGENOM	HOG000238648.
InParanoid	Q84VW9.
KO	K01595.
OMA	KFIPYLE.
PhylomeDB	Q84VW9.
ProtClustDB	CLSN2679806.
Enzyme and pathway databases
BioCyc	ARA:AT3G14940-MONOMER. MetaCyc:AT3G14940-MONOMER.
BRENDA	4.1.1.31. 399.
Gene expression databases
Genevestigator	Q84VW9.
GermOnline	AT3G14940. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR021135. PEP_COase. IPR018129. PEP_COase_AS. IPR022805. PEP_COase_bac/pln-type. IPR015813. Pyrv/PenolPyrv_Kinase-like_dom. [Graphical view]
Pfam	PF00311. PEPcase. 1 hit. [Graphical view]
PRINTS	PR00150. PEPCARBXLASE.
SUPFAM	SSF51621. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
PROSITE	PS00781. PEPCASE_1. 1 hit. PS00393. PEPCASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CAPP3_ARATH

Accession

Primary (citable) accession number: Q84VW9
Secondary accession number(s): O81357, Q0WTJ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	August 30, 2005
Last modified:	May 29, 2013
This is version 75 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program