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Protein

Phosphoenolpyruvate carboxylase 3

Gene

PPC3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Through the carboxylation of phosphoenolpyruvate (PEP) it forms oxaloacetate, a four-carbon dicarboxylic acid source for the tricarboxylic acid cycle.

Catalytic activityi

Phosphate + oxaloacetate = H2O + phosphoenolpyruvate + HCO3-.

Cofactori

Mg2+By similarity

Enzyme regulationi

By light-reversible phosphorylation.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei173By similarity1
Active sitei603By similarity1

GO - Molecular functioni

  • phosphoenolpyruvate carboxylase activity Source: TAIR

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processCarbon dioxide fixation, Photosynthesis
LigandMagnesium

Enzyme and pathway databases

BioCyciARA:AT3G14940-MONOMER.
MetaCyc:AT3G14940-MONOMER.
BRENDAi4.1.1.31. 399.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxylase 3 (EC:4.1.1.31)
Short name:
AtPPC3
Short name:
PEPC 3
Short name:
PEPCase 3
Gene namesi
Name:PPC3
Synonyms:PEPC, PPC
Ordered Locus Names:At3g14940
ORF Names:K15M2.8
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRilocus:2086315. AT3G14940.

Subcellular locationi

GO - Cellular componenti

  • apoplast Source: GO_Central
  • chloroplast Source: GO_Central
  • cytosol Source: TAIR

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001666591 – 968Phosphoenolpyruvate carboxylase 3Add BLAST968

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei11PhosphoserineBy similarity1
Modified residuei705PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ84VW9.
PRIDEiQ84VW9.

PTM databases

iPTMnetiQ84VW9.

Expressioni

Tissue specificityi

Expressed in roots and siliques, and to a lower extent in stems, leaves and flowers.1 Publication

Gene expression databases

GenevisibleiQ84VW9. AT.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

BioGridi6057. 3 interactors.
MINTiMINT-8060497.
STRINGi3702.AT3G14940.1.

Structurei

Secondary structure

1968
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi30 – 49Combined sources20
Helixi51 – 70Combined sources20
Helixi73 – 84Combined sources12
Helixi88 – 115Combined sources28
Helixi120 – 122Combined sources3
Helixi127 – 131Combined sources5
Turni133 – 135Combined sources3
Helixi139 – 150Combined sources12
Helixi154 – 162Combined sources9
Beta strandi165 – 170Combined sources6
Helixi180 – 196Combined sources17
Helixi203 – 221Combined sources19
Helixi233 – 240Combined sources8
Helixi243 – 246Combined sources4
Helixi248 – 264Combined sources17
Turni265 – 267Combined sources3
Beta strandi278 – 283Combined sources6
Turni285 – 287Combined sources3
Helixi297 – 325Combined sources29
Helixi333 – 340Combined sources8
Helixi366 – 391Combined sources26
Helixi398 – 400Combined sources3
Helixi405 – 421Combined sources17
Helixi425 – 428Combined sources4
Helixi431 – 442Combined sources12
Turni443 – 445Combined sources3
Beta strandi446 – 454Combined sources9
Helixi455 – 468Combined sources14
Helixi475 – 477Combined sources3
Helixi480 – 492Combined sources13
Helixi506 – 520Combined sources15
Helixi523 – 525Combined sources3
Beta strandi526 – 532Combined sources7
Helixi537 – 549Combined sources13
Beta strandi557 – 562Combined sources6
Helixi565 – 580Combined sources16
Helixi582 – 588Combined sources7
Beta strandi591 – 596Combined sources6
Helixi598 – 605Combined sources8
Helixi607 – 628Combined sources22
Beta strandi631 – 636Combined sources6
Helixi641 – 643Combined sources3
Helixi648 – 654Combined sources7
Beta strandi664 – 669Combined sources6
Helixi671 – 678Combined sources8
Helixi681 – 700Combined sources20
Helixi708 – 729Combined sources22
Helixi735 – 742Combined sources8
Helixi745 – 750Combined sources6
Turni766 – 768Combined sources3
Helixi771 – 780Combined sources10
Helixi785 – 788Combined sources4
Helixi791 – 801Combined sources11
Helixi805 – 815Combined sources11
Helixi817 – 831Combined sources15
Helixi835 – 845Combined sources11
Helixi848 – 850Combined sources3
Helixi851 – 872Combined sources22
Turni877 – 880Combined sources4
Helixi882 – 910Combined sources29
Helixi949 – 964Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5FDNX-ray2.20A/B1-968[»]
ProteinModelPortaliQ84VW9.
SMRiQ84VW9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PEPCase type 1 family.Curated

Phylogenomic databases

eggNOGiENOG410IEAR. Eukaryota.
COG2352. LUCA.
HOGENOMiHOG000238648.
InParanoidiQ84VW9.
KOiK01595.
OMAiCNDEVRI.
OrthoDBiEOG0936013M.
PhylomeDBiQ84VW9.

Family and domain databases

HAMAPiMF_00595. PEPcase_type1. 1 hit.
InterProiView protein in InterPro
IPR021135. PEP_COase.
IPR022805. PEP_COase_bac/pln-type.
IPR018129. PEP_COase_Lys_AS.
IPR033129. PEPCASE_His_AS.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
PfamiView protein in Pfam
PF00311. PEPcase. 1 hit.
PRINTSiPR00150. PEPCARBXLASE.
SUPFAMiSSF51621. SSF51621. 1 hit.
PROSITEiView protein in PROSITE
PS00781. PEPCASE_1. 1 hit.
PS00393. PEPCASE_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q84VW9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGRNIEKMA SIDAQLRQLV PAKVSEDDKL VEYDALLLDR FLDILQDLHG
60 70 80 90 100
EDLRETVQEL YELSAEYEGK REPSKLEELG SVLTSLDPGD SIVISKAFSH
110 120 130 140 150
MLNLANLAEE VQIAHRRRIK KLKKGDFVDE SSATTESDIE ETFKRLVSDL
160 170 180 190 200
GKSPEEIFDA LKNQTVDLVL TAHPTQSVRR SLLQKHGRIR DCLAQLYAKD
210 220 230 240 250
ITPDDKQELD ESLQREIQAA FRTDEIRRTP PTPQDEMRAG MSYFHETIWK
260 270 280 290 300
GVPKFLRRVD TALKNIGIDE RVPYNAPLIQ FSSWMGGDRD GNPRVTPEVT
310 320 330 340 350
RDVCLLARMM AANLYYNQIE NLMFELSMWR CTDEFRVRAD ELHRNSRKDA
360 370 380 390 400
AKHYIEFWKT IPPTEPYRVI LGDVRDKLYH TRERSRQLLS NGISDIPEEA
410 420 430 440 450
TFTNVEQFLE PLELCYRSLC SCGDSPIADG SLLDFLRQVS TFGLSLVRLD
460 470 480 490 500
IRQESERHTD VLDAITKHLD IGSSYRDWSE EGRQEWLLAE LSGKRPLFGP
510 520 530 540 550
DLPKTEEISD VLDTFKVISE LPSDCFGAYI ISMATSPSDV LAVELLQREC
560 570 580 590 600
HVKNPLRVVP LFEKLADLEA APAAVARLFS IDWYKNRING KQEVMIGYSD
610 620 630 640 650
SGKDAGRLSA AWELYKAQEE LVKVAKKYGV KLTMFHGRGG TVGRGGGPTH
660 670 680 690 700
LAILSQPPDT VNGSLRVTVQ GEVIEQSFGE AHLCFRTLQR FTAATLEHGM
710 720 730 740 750
NPPISPKPEW RALLDEMAVV ATEEYRSVVF QEPRFVEYFR LATPELEYGR
760 770 780 790 800
MNIGSRPSKR KPSGGIESLR AIPWIFAWTQ TRFHLPVWLG FGAAFRYAIK
810 820 830 840 850
KDVRNLHMLQ DMYKQWPFFR VTIDLIEMVF AKGDPGIAAL YDKLLVSEDL
860 870 880 890 900
WAFGEKLRAN FDETKNLVLQ TAGHKDLLEG DPYLKQRLRL RDSYITTLNV
910 920 930 940 950
CQAYTLKRIR DANYNVTLRP HISKEIMQSS KSAQELVKLN PTSEYAPGLE
960
DTLILTMKGI AAGLQNTG
Length:968
Mass (Da):110,160
Last modified:August 30, 2005 - v2
Checksum:i1CECE8B8981F63F0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti245H → R in AAO42888 (PubMed:14593172).Curated1
Sequence conflicti245H → R in BAE99551 (Ref. 6) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF071788 mRNA. Translation: AAC24594.1.
AJ131710 Genomic DNA. Translation: CAA10486.1.
AP000370 Genomic DNA. Translation: BAA97057.1.
CP002686 Genomic DNA. Translation: AEE75592.1.
CP002686 Genomic DNA. Translation: ANM64773.1.
BT004642 mRNA. Translation: AAO42888.1.
AK227556 mRNA. Translation: BAE99551.1.
PIRiT52186.
RefSeqiNP_001326778.1. NM_001338141.1.
NP_188112.1. NM_112356.4.
UniGeneiAt.24126.
At.74839.

Genome annotation databases

EnsemblPlantsiAT3G14940.1; AT3G14940.1; AT3G14940.
AT3G14940.2; AT3G14940.2; AT3G14940.
GeneIDi820723.
GrameneiAT3G14940.1; AT3G14940.1; AT3G14940.
AT3G14940.2; AT3G14940.2; AT3G14940.
KEGGiath:AT3G14940.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF071788 mRNA. Translation: AAC24594.1.
AJ131710 Genomic DNA. Translation: CAA10486.1.
AP000370 Genomic DNA. Translation: BAA97057.1.
CP002686 Genomic DNA. Translation: AEE75592.1.
CP002686 Genomic DNA. Translation: ANM64773.1.
BT004642 mRNA. Translation: AAO42888.1.
AK227556 mRNA. Translation: BAE99551.1.
PIRiT52186.
RefSeqiNP_001326778.1. NM_001338141.1.
NP_188112.1. NM_112356.4.
UniGeneiAt.24126.
At.74839.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5FDNX-ray2.20A/B1-968[»]
ProteinModelPortaliQ84VW9.
SMRiQ84VW9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi6057. 3 interactors.
MINTiMINT-8060497.
STRINGi3702.AT3G14940.1.

PTM databases

iPTMnetiQ84VW9.

Proteomic databases

PaxDbiQ84VW9.
PRIDEiQ84VW9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G14940.1; AT3G14940.1; AT3G14940.
AT3G14940.2; AT3G14940.2; AT3G14940.
GeneIDi820723.
GrameneiAT3G14940.1; AT3G14940.1; AT3G14940.
AT3G14940.2; AT3G14940.2; AT3G14940.
KEGGiath:AT3G14940.

Organism-specific databases

AraportiAT3G14940.
TAIRilocus:2086315. AT3G14940.

Phylogenomic databases

eggNOGiENOG410IEAR. Eukaryota.
COG2352. LUCA.
HOGENOMiHOG000238648.
InParanoidiQ84VW9.
KOiK01595.
OMAiCNDEVRI.
OrthoDBiEOG0936013M.
PhylomeDBiQ84VW9.

Enzyme and pathway databases

BioCyciARA:AT3G14940-MONOMER.
MetaCyc:AT3G14940-MONOMER.
BRENDAi4.1.1.31. 399.

Miscellaneous databases

PROiPR:Q84VW9.

Gene expression databases

GenevisibleiQ84VW9. AT.

Family and domain databases

HAMAPiMF_00595. PEPcase_type1. 1 hit.
InterProiView protein in InterPro
IPR021135. PEP_COase.
IPR022805. PEP_COase_bac/pln-type.
IPR018129. PEP_COase_Lys_AS.
IPR033129. PEPCASE_His_AS.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
PfamiView protein in Pfam
PF00311. PEPcase. 1 hit.
PRINTSiPR00150. PEPCARBXLASE.
SUPFAMiSSF51621. SSF51621. 1 hit.
PROSITEiView protein in PROSITE
PS00781. PEPCASE_1. 1 hit.
PS00393. PEPCASE_2. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCAPP3_ARATH
AccessioniPrimary (citable) accession number: Q84VW9
Secondary accession number(s): O81357, Q0WTJ7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: August 30, 2005
Last modified: May 10, 2017
This is version 103 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.