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Q84UI5

- MPK1_ORYSJ

UniProt

Q84UI5 - MPK1_ORYSJ

Protein

Mitogen-activated protein kinase 1

Gene

MPK1

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 82 (01 Oct 2014)
      Sequence version 1 (01 Jun 2003)
      Previous versions | rss
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    Functioni

    Involved in sphingolipid elicitor (SE)-dependent defense signaling pathway. Acts downstream of heterotrimeric G protein alpha subunit and small GTPase RAC1. May regulate the expression of various genes involved in biotic and abiotic stress response.1 Publication

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Enzyme regulationi

    Activated by threonine and tyrosine phosphorylation By similarity. Activated in response to sphingolipid elicitor (SE).By similarity1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei96 – 961ATPPROSITE-ProRule annotation
    Active sitei193 – 1931Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi73 – 819ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. MAP kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. camalexin biosynthetic process Source: EnsemblPlants/Gramene
    2. cell division Source: EnsemblPlants/Gramene
    3. induced systemic resistance, jasmonic acid mediated signaling pathway Source: EnsemblPlants/Gramene
    4. inflorescence development Source: EnsemblPlants/Gramene
    5. ovule development Source: EnsemblPlants/Gramene
    6. priming of cellular response to stress Source: EnsemblPlants/Gramene
    7. regulation of stomatal complex development Source: EnsemblPlants/Gramene
    8. regulation of stomatal complex patterning Source: EnsemblPlants/Gramene
    9. response to abscisic acid Source: EnsemblPlants/Gramene
    10. response to cold Source: EnsemblPlants/Gramene
    11. response to ethylene Source: EnsemblPlants/Gramene
    12. response to hydrogen peroxide Source: EnsemblPlants/Gramene
    13. response to salt stress Source: EnsemblPlants/Gramene
    14. response to UV-B Source: EnsemblPlants/Gramene
    15. root development Source: EnsemblPlants/Gramene

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Plant defense

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_205014. CREB phosphorylation through the activation of Ras.
    REACT_214549. Signalling to ERK5.
    REACT_215626. ERK2 activation.
    REACT_221522. ERKs are inactivated.
    REACT_221537. ERK1 activation.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mitogen-activated protein kinase 1 (EC:2.7.11.24)
    Short name:
    MAP kinase 1
    Alternative name(s):
    MAP kinase 6
    OsMAPK6
    OsSIPK
    Gene namesi
    Name:MPK1
    Synonyms:MAPK6, SIPK
    Ordered Locus Names:Os06g0154500, LOC_Os06g06090
    ORF Names:OSJNBa0085L11.14
    OrganismiOryza sativa subsp. japonica (Rice)
    Taxonomic identifieri39947 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
    ProteomesiUP000000763: Chromosome 6

    Organism-specific databases

    GrameneiQ84UI5.

    Subcellular locationi

    GO - Cellular componenti

    1. phragmoplast Source: EnsemblPlants/Gramene
    2. preprophase band Source: EnsemblPlants/Gramene
    3. trans-Golgi network Source: EnsemblPlants/Gramene

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 398398Mitogen-activated protein kinase 1PRO_0000239744Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei225 – 2251PhosphothreonineBy similarity
    Modified residuei227 – 2271PhosphotyrosineBy similarity

    Post-translational modificationi

    Dually phosphorylated on Thr-225 and Tyr-227, which activates the enzyme.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiQ84UI5.

    Interactioni

    Subunit structurei

    May interact with RAC1.

    Protein-protein interaction databases

    STRINGi39947.LOC_Os06g06090.1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ84UI5.
    SMRiQ84UI5. Positions 56-398.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini67 – 352286Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi225 – 2273TXY

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi23 – 3412Poly-AlaAdd
    BLAST

    Domaini

    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    HOGENOMiHOG000233024.
    KOiK14512.
    OMAiSDEPVCS.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR003527. MAP_kinase_CS.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS01351. MAPK. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q84UI5-1 [UniParc]FASTAAdd to Basket

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    MDAGAQPPDT EMAEAGGGQQ PPAAAAAAGA GAGAGMMENI QATLSHGGRF    50
    IQYNIFGNVF EVTAKYKPPI LPIGKGAYGI VCSALNSETG EQVAIKKIAN 100
    AFDNKIDAKR TLREIKLLRH MDHENIVAIR DIIPPPQRNS FNDVYIAYEL 150
    MDTDLHQIIR SNQALSEEHC QYFLYQILRG LKYIHSANVL HRDLKPSNLL 200
    LNANCDLKIC DFGLARTTSE TDFMTEYVVT RWYRAPELLL NSSEYTAAID 250
    VWSVGCIFME LMDRKPLFPG RDHVHQLRLL MELIGTPNEA DLDFVNENAR 300
    RYIRQLPRHA RQSFPEKFPH VHPLAIDLVE KMLTFDPRQR ITVEGALAHP 350
    YLASLHDISD EPVCSSPFSF DFEQHALSEE QMKDLIYQEG LAFNPDYQ 398
    Length:398
    Mass (Da):44,858
    Last modified:June 1, 2003 - v1
    Checksum:i46E2907463A7C490
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB183398 mRNA. Translation: BAD34534.1.
    AJ535841 mRNA. Translation: CAD59793.1.
    AP006533 Genomic DNA. Translation: BAD69291.1.
    RefSeqiNP_001056846.1. NM_001063381.1.
    UniGeneiOs.19011.

    Genome annotation databases

    EnsemblPlantsiOS06T0154500-01; OS06T0154500-01; OS06G0154500.
    GeneIDi4340170.
    KEGGiosa:4340170.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB183398 mRNA. Translation: BAD34534.1 .
    AJ535841 mRNA. Translation: CAD59793.1 .
    AP006533 Genomic DNA. Translation: BAD69291.1 .
    RefSeqi NP_001056846.1. NM_001063381.1.
    UniGenei Os.19011.

    3D structure databases

    ProteinModelPortali Q84UI5.
    SMRi Q84UI5. Positions 56-398.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 39947.LOC_Os06g06090.1.

    Proteomic databases

    PRIDEi Q84UI5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi OS06T0154500-01 ; OS06T0154500-01 ; OS06G0154500 .
    GeneIDi 4340170.
    KEGGi osa:4340170.

    Organism-specific databases

    Gramenei Q84UI5.

    Phylogenomic databases

    eggNOGi COG0515.
    HOGENOMi HOG000233024.
    KOi K14512.
    OMAi SDEPVCS.

    Enzyme and pathway databases

    Reactomei REACT_205014. CREB phosphorylation through the activation of Ras.
    REACT_214549. Signalling to ERK5.
    REACT_215626. ERK2 activation.
    REACT_221522. ERKs are inactivated.
    REACT_221537. ERK1 activation.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR003527. MAP_kinase_CS.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS01351. MAPK. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A sphingolipid elicitor-inducible mitogen-activated protein kinase is regulated by the small GTPase OsRac1 and heterotrimeric G-protein in rice."
      Lieberherr D., Thao N.P., Nakashima A., Umemura K., Kawasaki T., Shimamoto K.
      Plant Physiol. 138:1644-1652(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, ENZYME REGULATION, INTERACTION WITH RAC1.
      Strain: cv. Kinmaze.
    2. "Identification of a novel rice (Oryza sativa L. japonica-type cv. Nipponbare) mitogen-activated protein kinase OsSIPK, an orthologue of tobacco NtSIPK."
      Rakwal R., Jwa N.S., Shibato J., Iwahashi H.
      Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Nipponbare.
      Tissue: Leaf.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.
    4. "Molecular analysis of the rice MAP kinase gene family in relation to Magnaporthe grisea infection."
      Reyna N.S., Yang Y.
      Mol. Plant Microbe Interact. 19:530-540(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NOMENCLATURE.

    Entry informationi

    Entry nameiMPK1_ORYSJ
    AccessioniPrimary (citable) accession number: Q84UI5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 13, 2006
    Last sequence update: June 1, 2003
    Last modified: October 1, 2014
    This is version 82 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Oryza sativa (rice)
      Index of Oryza sativa entries and their corresponding gene designations
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3