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Q84UI5 (MPK1_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mitogen-activated protein kinase 1

Short name=MAP kinase 1
EC=2.7.11.24
Alternative name(s):
MAP kinase 6
OsMAPK6
OsSIPK
Gene names
Name:MPK1
Synonyms:MAPK6, SIPK
Ordered Locus Names:Os06g0154500, LOC_Os06g06090
ORF Names:OSJNBa0085L11.14
OrganismOryza sativa subsp. japonica (Rice) [Reference proteome]
Taxonomic identifier39947 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza

Protein attributes

Sequence length398 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in sphingolipid elicitor (SE)-dependent defense signaling pathway. Acts downstream of heterotrimeric G protein alpha subunit and small GTPase RAC1. May regulate the expression of various genes involved in biotic and abiotic stress response. Ref.1

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulation

Activated by threonine and tyrosine phosphorylation By similarity. Activated in response to sphingolipid elicitor (SE). Ref.1

Subunit structure

May interact with RAC1.

Domain

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Post-translational modification

Dually phosphorylated on Thr-225 and Tyr-227, which activates the enzyme By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.

Contains 1 protein kinase domain.

Ontologies

Keywords
   Biological processPlant defense
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Serine/threonine-protein kinase
Transferase
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcamalexin biosynthetic process

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

cell division

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

induced systemic resistance, jasmonic acid mediated signaling pathway

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

inflorescence development

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

ovule development

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

priming of cellular response to stress

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

regulation of stomatal complex development

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

regulation of stomatal complex patterning

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

response to UV-B

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

response to abscisic acid

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

response to cold

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

response to ethylene

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

response to hydrogen peroxide

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

response to salt stress

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

root development

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

   Cellular_componentphragmoplast

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

preprophase band

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

trans-Golgi network

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

MAP kinase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 398398Mitogen-activated protein kinase 1
PRO_0000239744

Regions

Domain67 – 352286Protein kinase
Nucleotide binding73 – 819ATP By similarity
Motif225 – 2273TXY
Compositional bias23 – 3412Poly-Ala

Sites

Active site1931Proton acceptor By similarity
Binding site961ATP By similarity

Amino acid modifications

Modified residue2251Phosphothreonine By similarity
Modified residue2271Phosphotyrosine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q84UI5 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 46E2907463A7C490

FASTA39844,858
        10         20         30         40         50         60 
MDAGAQPPDT EMAEAGGGQQ PPAAAAAAGA GAGAGMMENI QATLSHGGRF IQYNIFGNVF 

        70         80         90        100        110        120 
EVTAKYKPPI LPIGKGAYGI VCSALNSETG EQVAIKKIAN AFDNKIDAKR TLREIKLLRH 

       130        140        150        160        170        180 
MDHENIVAIR DIIPPPQRNS FNDVYIAYEL MDTDLHQIIR SNQALSEEHC QYFLYQILRG 

       190        200        210        220        230        240 
LKYIHSANVL HRDLKPSNLL LNANCDLKIC DFGLARTTSE TDFMTEYVVT RWYRAPELLL 

       250        260        270        280        290        300 
NSSEYTAAID VWSVGCIFME LMDRKPLFPG RDHVHQLRLL MELIGTPNEA DLDFVNENAR 

       310        320        330        340        350        360 
RYIRQLPRHA RQSFPEKFPH VHPLAIDLVE KMLTFDPRQR ITVEGALAHP YLASLHDISD 

       370        380        390 
EPVCSSPFSF DFEQHALSEE QMKDLIYQEG LAFNPDYQ 

« Hide

References

« Hide 'large scale' references
[1]"A sphingolipid elicitor-inducible mitogen-activated protein kinase is regulated by the small GTPase OsRac1 and heterotrimeric G-protein in rice."
Lieberherr D., Thao N.P., Nakashima A., Umemura K., Kawasaki T., Shimamoto K.
Plant Physiol. 138:1644-1652(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, ENZYME REGULATION, INTERACTION WITH RAC1.
Strain: cv. Kinmaze.
[2]"Identification of a novel rice (Oryza sativa L. japonica-type cv. Nipponbare) mitogen-activated protein kinase OsSIPK, an orthologue of tobacco NtSIPK."
Rakwal R., Jwa N.S., Shibato J., Iwahashi H.
Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Nipponbare.
Tissue: Leaf.
[3]"The map-based sequence of the rice genome."
International rice genome sequencing project (IRGSP)
Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[4]"Molecular analysis of the rice MAP kinase gene family in relation to Magnaporthe grisea infection."
Reyna N.S., Yang Y.
Mol. Plant Microbe Interact. 19:530-540(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NOMENCLATURE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB183398 mRNA. Translation: BAD34534.1.
AJ535841 mRNA. Translation: CAD59793.1.
AP006533 Genomic DNA. Translation: BAD69291.1.
RefSeqNP_001056846.1. NM_001063381.1.
UniGeneOs.19011.

3D structure databases

ProteinModelPortalQ84UI5.
SMRQ84UI5. Positions 56-398.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING39947.LOC_Os06g06090.1.

Proteomic databases

PRIDEQ84UI5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsOS06T0154500-01; OS06T0154500-01; OS06G0154500.
GeneID4340170.
KEGGosa:4340170.

Organism-specific databases

GrameneQ84UI5.

Phylogenomic databases

eggNOGCOG0515.
HOGENOMHOG000233024.
KOK14512.
OMASDEPVCS.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMPK1_ORYSJ
AccessionPrimary (citable) accession number: Q84UI5
Entry history
Integrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: June 1, 2003
Last modified: May 14, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Oryza sativa (rice)

Index of Oryza sativa entries and their corresponding gene designations