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Protein

Glutathione S-transferase F4

Gene

GSTF4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

May be involved in the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles and have a detoxification role against certain herbicides.By similarity

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.

GO - Molecular functioni

  1. glutathione transferase activity Source: UniProtKB-EC

GO - Biological processi

  1. response to toxic substance Source: UniProtKB-KW
  2. toxin catabolic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Detoxification

Enzyme and pathway databases

BioCyciARA:AT1G02950-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione S-transferase F4 (EC:2.5.1.18)
Short name:
AtGSTF4
Alternative name(s):
GST class-phi member 4
Glutathione S-transferase 31
Gene namesi
Name:GSTF4
Synonyms:GST31
Ordered Locus Names:At1g02950
ORF Names:F22D16.5
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G02950.

Subcellular locationi

Cytoplasmcytosol Curated

GO - Cellular componenti

  1. cytoplasm Source: TAIR
  2. cytosol Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 245245Glutathione S-transferase F4PRO_0000413542Add
BLAST

Proteomic databases

PRIDEiQ84TK0.

Expressioni

Gene expression databases

ExpressionAtlasiQ84TK0. baseline and differential.
GenevestigatoriQ84TK0.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT1G02950.2-P.

Structurei

3D structure databases

ProteinModelPortaliQ84TK0.
SMRiQ84TK0. Positions 26-228.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini25 – 10682GST N-terminalAdd
BLAST
Domaini114 – 244131GST C-terminalAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni35 – 362Glutathione bindingBy similarity
Regioni64 – 652Glutathione bindingBy similarity
Regioni77 – 782Glutathione bindingBy similarity
Regioni90 – 912Glutathione bindingBy similarity

Sequence similaritiesi

Belongs to the GST superfamily. Phi family.Curated
Contains 1 GST C-terminal domain.Curated
Contains 1 GST N-terminal domain.Curated

Phylogenomic databases

HOGENOMiHOG000125746.
InParanoidiQ84TK0.
KOiK00799.
OMAiMLGAPTD.
PhylomeDBiQ84TK0.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF00043. GST_C. 1 hit.
PF13417. GST_N_3. 1 hit.
[Graphical view]
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q84TK0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDCLQMVFKL FPNWKREAEV KKLVAGYKVH GDPFSTNTRR VLAVLHEKRL
60 70 80 90 100
SYEPITVKLQ TGEHKTEPFL SLNPFGQVPV FEDGSVKLYE SRAITQYIAY
110 120 130 140 150
VHSSRGTQLL NLRSHETMAT LTMWMEIEAH QFDPPASKLT WEQVIKPIYG
160 170 180 190 200
LETDQTIVKE NEAILEKVLN IYEKRLEESR FLACNSFTLV DLHHLPNIQY
210 220 230 240
LLGTPTKKLF EKRSKVRKWV DEITSREAWK MACDQEKSWF NKPRN
Length:245
Mass (Da):28,701
Last modified:June 1, 2003 - v1
Checksum:iA9075D1B448C4886
GO
Isoform 2 (identifier: Q84TK0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     24-25: Missing.

Show »
Length:243
Mass (Da):28,531
Checksum:iB1A834CB5E15B48D
GO

Sequence cautioni

The sequence AAF02871.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei24 – 252Missing in isoform 2. 3 PublicationsVSP_041937

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC009525 Genomic DNA. Translation: AAF02871.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE27500.1.
CP002684 Genomic DNA. Translation: AEE27501.1.
CP002684 Genomic DNA. Translation: AEE27502.1.
AF320055 mRNA. Translation: AAG40875.1.
BT005712 mRNA. Translation: AAO64132.1.
BT020399 mRNA. Translation: AAV97790.1.
AK228359 mRNA. Translation: BAF00298.1.
PIRiA86160.
RefSeqiNP_001030937.1. NM_001035860.1. [Q84TK0-2]
NP_563670.1. NM_100176.3. [Q84TK0-2]
NP_849581.1. NM_179250.1. [Q84TK0-1]
UniGeneiAt.24805.

Genome annotation databases

EnsemblPlantsiAT1G02950.2; AT1G02950.2; AT1G02950. [Q84TK0-1]
GeneIDi838240.
KEGGiath:AT1G02950.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC009525 Genomic DNA. Translation: AAF02871.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE27500.1.
CP002684 Genomic DNA. Translation: AEE27501.1.
CP002684 Genomic DNA. Translation: AEE27502.1.
AF320055 mRNA. Translation: AAG40875.1.
BT005712 mRNA. Translation: AAO64132.1.
BT020399 mRNA. Translation: AAV97790.1.
AK228359 mRNA. Translation: BAF00298.1.
PIRiA86160.
RefSeqiNP_001030937.1. NM_001035860.1. [Q84TK0-2]
NP_563670.1. NM_100176.3. [Q84TK0-2]
NP_849581.1. NM_179250.1. [Q84TK0-1]
UniGeneiAt.24805.

3D structure databases

ProteinModelPortaliQ84TK0.
SMRiQ84TK0. Positions 26-228.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT1G02950.2-P.

Proteomic databases

PRIDEiQ84TK0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G02950.2; AT1G02950.2; AT1G02950. [Q84TK0-1]
GeneIDi838240.
KEGGiath:AT1G02950.

Organism-specific databases

TAIRiAT1G02950.

Phylogenomic databases

HOGENOMiHOG000125746.
InParanoidiQ84TK0.
KOiK00799.
OMAiMLGAPTD.
PhylomeDBiQ84TK0.

Enzyme and pathway databases

BioCyciARA:AT1G02950-MONOMER.

Gene expression databases

ExpressionAtlasiQ84TK0. baseline and differential.
GenevestigatoriQ84TK0.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF00043. GST_C. 1 hit.
PF13417. GST_N_3. 1 hit.
[Graphical view]
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Probing the diversity of the Arabidopsis glutathione S-transferase gene family."
    Wagner U., Edwards R., Dixon D.P., Mauch F.
    Plant Mol. Biol. 49:515-532(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), GENE FAMILY, NOMENCLATURE.
    Strain: cv. Columbia.
  2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: cv. Columbia.
  5. "Arabidopsis ORF clones."
    Shinn P., Chen H., Cheuk R.F., Kim C.J., Ecker J.R.
    Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: cv. Columbia.
  6. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.

Entry informationi

Entry nameiGSTF4_ARATH
AccessioniPrimary (citable) accession number: Q84TK0
Secondary accession number(s): Q9FPM2, Q9SRY7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 19, 2011
Last sequence update: June 1, 2003
Last modified: January 7, 2015
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.