Q84TK0 (GSTF4_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutathione S-transferase F4
Short name=AtGSTF4
EC=2.5.1.18

Alternative name(s):
GST class-phi member 4
Glutathione S-transferase 31

Gene names

Name:	GSTF4
Synonyms:	GST31
Ordered Locus Names:	At1g02950
ORF Names:	F22D16.5

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	245 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May be involved in the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles and have a detoxification role against certain herbicides By similarity.
Catalytic activity	RX + glutathione = HX + R-S-glutathione.
Subcellular location	Cytoplasm › cytosol Probable.
Sequence similarities	Belongs to the GST superfamily. Phi family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.
Sequence caution	The sequence AAF02871.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Detoxification
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	response to toxin Inferred from electronic annotation. Source: UniProtKB-KW toxin catabolic process Traceable author statement Ref.1. Source: TAIR
Cellular_component	cytoplasm Non-traceable author statement Ref.1. Source: TAIR cytosol Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	glutathione transferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q84TK0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q84TK0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     24-25: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 245	245	Glutathione S-transferase F4		PRO_0000413542
Regions
	Domain	25 – 106	82	GST N-terminal
	Domain	114 – 244	131	GST C-terminal
	Region	35 – 36	2	Glutathione binding By similarity
	Region	64 – 65	2	Glutathione binding By similarity
	Region	77 – 78	2	Glutathione binding By similarity
	Region	90 – 91	2	Glutathione binding By similarity
Natural variations
	Alternative sequence	24 – 25	2	Missing in isoform 2.		VSP_041937

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: A9075D1B448C4886	FASTA	245	28,701
	10 20 30 40 50 60 MDCLQMVFKL FPNWKREAEV KKLVAGYKVH GDPFSTNTRR VLAVLHEKRL SYEPITVKLQ 70 80 90 100 110 120 TGEHKTEPFL SLNPFGQVPV FEDGSVKLYE SRAITQYIAY VHSSRGTQLL NLRSHETMAT 130 140 150 160 170 180 LTMWMEIEAH QFDPPASKLT WEQVIKPIYG LETDQTIVKE NEAILEKVLN IYEKRLEESR 190 200 210 220 230 240 FLACNSFTLV DLHHLPNIQY LLGTPTKKLF EKRSKVRKWV DEITSREAWK MACDQEKSWF NKPRN
	Isoform 2 [UniParc]. Checksum: B1A834CB5E15B48D	FASTA	243	28,531
	10 20 30 40 50 60 MDCLQMVFKL FPNWKREAEV KKLGYKVHGD PFSTNTRRVL AVLHEKRLSY EPITVKLQTG 70 80 90 100 110 120 EHKTEPFLSL NPFGQVPVFE DGSVKLYESR AITQYIAYVH SSRGTQLLNL RSHETMATLT 130 140 150 160 170 180 MWMEIEAHQF DPPASKLTWE QVIKPIYGLE TDQTIVKENE AILEKVLNIY EKRLEESRFL 190 200 210 220 230 240 ACNSFTLVDL HHLPNIQYLL GTPTKKLFEK RSKVRKWVDE ITSREAWKMA CDQEKSWFNK PRN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Probing the diversity of the Arabidopsis glutathione S-transferase gene family." Wagner U., Edwards R., Dixon D.P., Mauch F. Plant Mol. Biol. 49:515-532(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), GENE FAMILY, NOMENCLATURE. Strain: cv. Columbia.
[2]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: cv. Columbia.
[5]	"Arabidopsis ORF clones." Shinn P., Chen H., Cheuk R.F., Kim C.J., Ecker J.R. Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: cv. Columbia.
[6]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC009525 Genomic DNA. Translation: AAF02871.1. Sequence problems. CP002684 Genomic DNA. Translation: AEE27500.1. CP002684 Genomic DNA. Translation: AEE27501.1. CP002684 Genomic DNA. Translation: AEE27502.1. AF320055 mRNA. Translation: AAG40875.1. BT005712 mRNA. Translation: AAO64132.1. BT020399 mRNA. Translation: AAV97790.1. AK228359 mRNA. Translation: BAF00298.1.
IPI	IPI00533840.
PIR	A86160.
RefSeq	NP_001030937.1. NM_001035860.1. NP_563670.1. NM_100176.3. NP_849581.1. NM_179250.1.
UniGene	At.24805.
3D structure databases
HSSP	HSSP built from PDB template 1GNW based on UniProtKB P46422.
ProteinModelPortal	Q84TK0.
SMR	Q84TK0. Positions 26-228.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT1G02950.2-P.
Proteomic databases
PRIDE	Q84TK0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G02950.2; AT1G02950.2; AT1G02950.
GeneID	838240.
KEGG	ath:AT1G02950.
Organism-specific databases
TAIR	At1g02950.
Phylogenomic databases
HOGENOM	HOG000125746.
InParanoid	Q84TK0.
KO	K00799.
OMA	FAMLDDE.
PhylomeDB	Q84TK0.
ProtClustDB	CLSN2679614.
Gene expression databases
ArrayExpress	Q84TK0.
Genevestigator	Q84TK0.
Family and domain databases
Gene3D	1.20.1050.10. 1 hit. 3.40.30.10. 1 hit.
InterPro	IPR010987. Glutathione-S-Trfase_C-like. IPR004045. Glutathione_S-Trfase_N. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR004046. GST_C. IPR012336. Thioredoxin-like_fold. [Graphical view]
Pfam	PF00043. GST_C. 1 hit. [Graphical view]
SUPFAM	SSF47616. GST_C_like. 1 hit. SSF52833. Thiordxn-like_fd. 1 hit.
PROSITE	PS50405. GST_CTER. 1 hit. PS50404. GST_NTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSTF4_ARATH

Accession

Primary (citable) accession number: Q84TK0
Secondary accession number(s): Q9FPM2, Q9SRY7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 19, 2011
Last sequence update:	June 1, 2003
Last modified:	May 1, 2013
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program