Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Cyclin-dependent kinase E-1

Gene

CDKE-1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in cell differentiation. Required for the specification of stamen and carpel identities and for the proper termination of stem cells in the floral meristem.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.
ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei55 – 551ATPPROSITE-ProRule annotation
Active sitei154 – 1541Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi31 – 399ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. cyclin-dependent protein serine/threonine kinase activity Source: UniProtKB-EC
  3. RNA polymerase II carboxy-terminal domain kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. cell cycle Source: UniProtKB-KW
  2. cell division Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Cell cycle, Cell division

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT5G63610-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclin-dependent kinase E-1 (EC:2.7.11.22, EC:2.7.11.23)
Short name:
CDKE;1
Alternative name(s):
Cyclin-dependent kinase 8
Protein HUA ENHANCER 3
Gene namesi
Name:CDKE-1
Synonyms:CDK8, HEN3
Ordered Locus Names:At5g63610
ORF Names:MBK5.8
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G63610.

Subcellular locationi

Nucleus Curated

GO - Cellular componenti

  1. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 470470Cyclin-dependent kinase E-1PRO_0000293122Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei36 – 361PhosphotyrosineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ84TI6.
PRIDEiQ84TI6.

Expressioni

Tissue specificityi

Expressed in roots, leaves and stems. Expressed in young dividing tissue, such as shoot and root tips, lateral root primordia, young leaves and flowers. Expressed in the inflorescence meristem, inflorescence stem and young flowers.1 Publication

Gene expression databases

GenevestigatoriQ84TI6.

Interactioni

Subunit structurei

Interacts with MED14, HDA19 and LUG.1 Publication

Protein-protein interaction databases

BioGridi21723. 22 interactions.
IntActiQ84TI6. 4 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ84TI6.
SMRiQ84TI6. Positions 13-413.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini25 – 333309Protein kinasePROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi445 – 4484Poly-Gln

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000233024.
InParanoidiQ84TI6.
KOiK02208.
OMAiAVDCWAV.
PhylomeDBiQ84TI6.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q84TI6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGDGSSSRSN SSNSTSEKPE WLQQYNLVGK IGEGTYGLVF LARTKTPPKR
60 70 80 90 100
PIAIKKFKQS KDGDGVSPTA IREIMLLREI SHENVVKLVN VHINFADMSL
110 120 130 140 150
YLAFDYAEYD LYEIIRHHRD KVGHSLNTYT VKSLLWQLLN GLNYLHSNWI
160 170 180 190 200
IHRDLKPSNI LVMGDAEEHG IVKIADFGLA RIYQAPLKPL SDNGVVVTIW
210 220 230 240 250
YRAPELLLGS KHYTSAVDMW AVGCIFAELL TLKPLFQGAE AKSSQNPFQL
260 270 280 290 300
DQLDKIFKIL GHPTMDKWPT LVNLPHWQND VQHIQAHKYD SVGLHNVVHL
310 320 330 340 350
NQKSPAYDLL SKMLEYDPLK RITASQALEH EYFRMDPLPG RNAFVASQPM
360 370 380 390 400
EKNVNYPTRP VDTNTDFEGT TSINPPQAVA AGNVAGNMAG AHGMGSRSMP
410 420 430 440 450
RPMVAHNMQR MQQSQGMMAY NFPAQAGLNP SVPLQQQRGM AQPHQQQQLR
460 470
RKDPGMGMSG YAPPNKSRRL
Length:470
Mass (Da):52,797
Last modified:July 9, 2007 - v2
Checksum:i85A92D0000FD5E46
GO

Sequence cautioni

The sequence CAD29165.1 differs from that shown. Reason: Frameshift at position 401. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti452 – 4521K → E in AAO64158 (PubMed:14593172).Curated
Sequence conflicti452 – 4521K → E in BAF00561 (Ref. 6) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY600243 mRNA. Translation: AAT36644.1.
AB005234 Genomic DNA. Translation: BAB10454.1.
CP002688 Genomic DNA. Translation: AED97776.1.
BT005753 mRNA. Translation: AAO64158.1.
BT020376 mRNA. Translation: AAV85731.1.
AK228654 mRNA. Translation: BAF00561.1.
AJ439879 mRNA. Translation: CAD29165.1. Frameshift.
RefSeqiNP_201166.1. NM_125756.4.
UniGeneiAt.28990.

Genome annotation databases

EnsemblPlantsiAT5G63610.1; AT5G63610.1; AT5G63610.
GeneIDi836481.
KEGGiath:AT5G63610.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY600243 mRNA. Translation: AAT36644.1.
AB005234 Genomic DNA. Translation: BAB10454.1.
CP002688 Genomic DNA. Translation: AED97776.1.
BT005753 mRNA. Translation: AAO64158.1.
BT020376 mRNA. Translation: AAV85731.1.
AK228654 mRNA. Translation: BAF00561.1.
AJ439879 mRNA. Translation: CAD29165.1. Frameshift.
RefSeqiNP_201166.1. NM_125756.4.
UniGeneiAt.28990.

3D structure databases

ProteinModelPortaliQ84TI6.
SMRiQ84TI6. Positions 13-413.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi21723. 22 interactions.
IntActiQ84TI6. 4 interactions.

Proteomic databases

PaxDbiQ84TI6.
PRIDEiQ84TI6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G63610.1; AT5G63610.1; AT5G63610.
GeneIDi836481.
KEGGiath:AT5G63610.

Organism-specific databases

GeneFarmi3295. 110.
TAIRiAT5G63610.

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000233024.
InParanoidiQ84TI6.
KOiK02208.
OMAiAVDCWAV.
PhylomeDBiQ84TI6.

Enzyme and pathway databases

BioCyciARA:AT5G63610-MONOMER.

Gene expression databases

GenevestigatoriQ84TI6.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "HUA ENHANCER3 reveals a role for a cyclin-dependent protein kinase in the specification of floral organ identity in Arabidopsis."
    Wang W., Chen X.
    Development 131:3147-3156(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
  2. "Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence features of the 1.6 Mb regions covered by twenty physically assigned P1 clones."
    Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M., Miyajima N., Tabata S.
    DNA Res. 4:215-230(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Arabidopsis ORF clones."
    Cheuk R.F., Chen H., Kim C.J., Shinn P., Ecker J.R.
    Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  7. "The identification of Arabidopsis cyclin-dependent kinase 8."
    Pu S.Y., Huang H.J.
    Submitted (FEB-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 170-470.
  8. "Genome-wide analysis of core cell cycle genes in Arabidopsis."
    Vandepoele K., Raes J., de Veylder L., Rouze P., Rombauts S., Inze D.
    Plant Cell 14:903-916(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  9. "Cell cycle regulation in plant development."
    Inze D., de Veylder L.
    Annu. Rev. Genet. 40:77-105(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.
  10. "The transcription corepressor LEUNIG interacts with the histone deacetylase HDA19 and mediator components MED14 (SWP) and CDK8 (HEN3) to repress transcription."
    Gonzalez D., Bowen A.J., Carroll T.S., Conlan R.S.
    Mol. Cell. Biol. 27:5306-5315(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MED14; LUG AND HDA19.

Entry informationi

Entry nameiCDKE1_ARATH
AccessioniPrimary (citable) accession number: Q84TI6
Secondary accession number(s): Q70Z09, Q9FFQ3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 9, 2007
Last sequence update: July 9, 2007
Last modified: March 3, 2015
This is version 94 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.