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Protein

Cyclin-dependent kinase E-1

Gene

CDKE-1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in cell differentiation. Required for the specification of stamen and carpel identities and for the proper termination of stem cells in the floral meristem.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.
ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei55ATPPROSITE-ProRule annotation1
Active sitei154Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi31 – 39ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processCell cycle, Cell division
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclin-dependent kinase E-1 (EC:2.7.11.22, EC:2.7.11.23)
Short name:
CDKE;1
Alternative name(s):
Cyclin-dependent kinase 8
Protein HUA ENHANCER 3
Gene namesi
Name:CDKE-1
Synonyms:CDK8, HEN3
Ordered Locus Names:At5g63610
ORF Names:MBK5.8
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G63610
TAIRilocus:2160609 AT5G63610

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002931221 – 470Cyclin-dependent kinase E-1Add BLAST470

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei36PhosphotyrosineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ84TI6
PRIDEiQ84TI6

Expressioni

Tissue specificityi

Expressed in roots, leaves and stems. Expressed in young dividing tissue, such as shoot and root tips, lateral root primordia, young leaves and flowers. Expressed in the inflorescence meristem, inflorescence stem and young flowers.1 Publication

Gene expression databases

ExpressionAtlasiQ84TI6 baseline and differential
GenevisibleiQ84TI6 AT

Interactioni

Subunit structurei

Interacts with MED14, HDA19 and LUG.1 Publication

Protein-protein interaction databases

BioGridi21723, 26 interactors
IntActiQ84TI6, 4 interactors
STRINGi3702.AT5G63610.1

Structurei

3D structure databases

ProteinModelPortaliQ84TI6
SMRiQ84TI6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini25 – 333Protein kinasePROSITE-ProRule annotationAdd BLAST309

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi445 – 448Poly-Gln4

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0666 Eukaryota
ENOG410XPPA LUCA
HOGENOMiHOG000233024
InParanoidiQ84TI6
KOiK02208
OMAiKSIYMVF
OrthoDBiEOG09360AQR
PhylomeDBiQ84TI6

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequencei

Sequence statusi: Complete.

Q84TI6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGDGSSSRSN SSNSTSEKPE WLQQYNLVGK IGEGTYGLVF LARTKTPPKR
60 70 80 90 100
PIAIKKFKQS KDGDGVSPTA IREIMLLREI SHENVVKLVN VHINFADMSL
110 120 130 140 150
YLAFDYAEYD LYEIIRHHRD KVGHSLNTYT VKSLLWQLLN GLNYLHSNWI
160 170 180 190 200
IHRDLKPSNI LVMGDAEEHG IVKIADFGLA RIYQAPLKPL SDNGVVVTIW
210 220 230 240 250
YRAPELLLGS KHYTSAVDMW AVGCIFAELL TLKPLFQGAE AKSSQNPFQL
260 270 280 290 300
DQLDKIFKIL GHPTMDKWPT LVNLPHWQND VQHIQAHKYD SVGLHNVVHL
310 320 330 340 350
NQKSPAYDLL SKMLEYDPLK RITASQALEH EYFRMDPLPG RNAFVASQPM
360 370 380 390 400
EKNVNYPTRP VDTNTDFEGT TSINPPQAVA AGNVAGNMAG AHGMGSRSMP
410 420 430 440 450
RPMVAHNMQR MQQSQGMMAY NFPAQAGLNP SVPLQQQRGM AQPHQQQQLR
460 470
RKDPGMGMSG YAPPNKSRRL
Length:470
Mass (Da):52,797
Last modified:July 10, 2007 - v2
Checksum:i85A92D0000FD5E46
GO

Sequence cautioni

The sequence CAD29165 differs from that shown. Reason: Frameshift at position 401.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti452K → E in AAO64158 (PubMed:14593172).Curated1
Sequence conflicti452K → E in BAF00561 (Ref. 6) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY600243 mRNA Translation: AAT36644.1
AB005234 Genomic DNA Translation: BAB10454.1
CP002688 Genomic DNA Translation: AED97776.1
BT005753 mRNA Translation: AAO64158.1
BT020376 mRNA Translation: AAV85731.1
AK228654 mRNA Translation: BAF00561.1
AJ439879 mRNA Translation: CAD29165.1 Frameshift.
RefSeqiNP_201166.1, NM_125756.5
UniGeneiAt.28990

Genome annotation databases

EnsemblPlantsiAT5G63610.1; AT5G63610.1; AT5G63610
GeneIDi836481
GrameneiAT5G63610.1; AT5G63610.1; AT5G63610
KEGGiath:AT5G63610

Similar proteinsi

Entry informationi

Entry nameiCDKE1_ARATH
AccessioniPrimary (citable) accession number: Q84TI6
Secondary accession number(s): Q70Z09, Q9FFQ3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: July 10, 2007
Last modified: April 25, 2018
This is version 112 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health