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Q84TI6

- CDKE1_ARATH

UniProt

Q84TI6 - CDKE1_ARATH

Protein

Cyclin-dependent kinase E-1

Gene

CDKE-1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 91 (01 Oct 2014)
      Sequence version 2 (10 Jul 2007)
      Previous versions | rss
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    Functioni

    Involved in cell differentiation. Required for the specification of stamen and carpel identities and for the proper termination of stem cells in the floral meristem.1 Publication

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.
    ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei55 – 551ATPPROSITE-ProRule annotation
    Active sitei154 – 1541Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi31 – 399ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. cyclin-dependent protein serine/threonine kinase activity Source: UniProtKB-EC
    3. protein binding Source: UniProtKB
    4. RNA polymerase II carboxy-terminal domain kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cell cycle Source: UniProtKB-KW
    2. cell division Source: UniProtKB-KW

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Cell cycle, Cell division

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT5G63610-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cyclin-dependent kinase E-1 (EC:2.7.11.22, EC:2.7.11.23)
    Short name:
    CDKE;1
    Alternative name(s):
    Cyclin-dependent kinase 8
    Protein HUA ENHANCER 3
    Gene namesi
    Name:CDKE-1
    Synonyms:CDK8, HEN3
    Ordered Locus Names:At5g63610
    ORF Names:MBK5.8
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G63610.

    Subcellular locationi

    Nucleus Curated

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 470470Cyclin-dependent kinase E-1PRO_0000293122Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei36 – 361PhosphotyrosineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ84TI6.
    PRIDEiQ84TI6.

    Expressioni

    Tissue specificityi

    Expressed in roots, leaves and stems. Expressed in young dividing tissue, such as shoot and root tips, lateral root primordia, young leaves and flowers. Expressed in the inflorescence meristem, inflorescence stem and young flowers.1 Publication

    Gene expression databases

    GenevestigatoriQ84TI6.

    Interactioni

    Subunit structurei

    Interacts with MED14, HDA19 and LUG.1 Publication

    Protein-protein interaction databases

    BioGridi21723. 22 interactions.
    IntActiQ84TI6. 4 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ84TI6.
    SMRiQ84TI6. Positions 13-413.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini25 – 333309Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi445 – 4484Poly-Gln

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    HOGENOMiHOG000233024.
    InParanoidiQ84TI6.
    KOiK02208.
    OMAiRSMPRPM.
    PhylomeDBiQ84TI6.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q84TI6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGDGSSSRSN SSNSTSEKPE WLQQYNLVGK IGEGTYGLVF LARTKTPPKR    50
    PIAIKKFKQS KDGDGVSPTA IREIMLLREI SHENVVKLVN VHINFADMSL 100
    YLAFDYAEYD LYEIIRHHRD KVGHSLNTYT VKSLLWQLLN GLNYLHSNWI 150
    IHRDLKPSNI LVMGDAEEHG IVKIADFGLA RIYQAPLKPL SDNGVVVTIW 200
    YRAPELLLGS KHYTSAVDMW AVGCIFAELL TLKPLFQGAE AKSSQNPFQL 250
    DQLDKIFKIL GHPTMDKWPT LVNLPHWQND VQHIQAHKYD SVGLHNVVHL 300
    NQKSPAYDLL SKMLEYDPLK RITASQALEH EYFRMDPLPG RNAFVASQPM 350
    EKNVNYPTRP VDTNTDFEGT TSINPPQAVA AGNVAGNMAG AHGMGSRSMP 400
    RPMVAHNMQR MQQSQGMMAY NFPAQAGLNP SVPLQQQRGM AQPHQQQQLR 450
    RKDPGMGMSG YAPPNKSRRL 470
    Length:470
    Mass (Da):52,797
    Last modified:July 10, 2007 - v2
    Checksum:i85A92D0000FD5E46
    GO

    Sequence cautioni

    The sequence CAD29165.1 differs from that shown. Reason: Frameshift at position 401.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti452 – 4521K → E in AAO64158. (PubMed:14593172)Curated
    Sequence conflicti452 – 4521K → E in BAF00561. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY600243 mRNA. Translation: AAT36644.1.
    AB005234 Genomic DNA. Translation: BAB10454.1.
    CP002688 Genomic DNA. Translation: AED97776.1.
    BT005753 mRNA. Translation: AAO64158.1.
    BT020376 mRNA. Translation: AAV85731.1.
    AK228654 mRNA. Translation: BAF00561.1.
    AJ439879 mRNA. Translation: CAD29165.1. Frameshift.
    RefSeqiNP_201166.1. NM_125756.4.
    UniGeneiAt.28990.

    Genome annotation databases

    EnsemblPlantsiAT5G63610.1; AT5G63610.1; AT5G63610.
    GeneIDi836481.
    KEGGiath:AT5G63610.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY600243 mRNA. Translation: AAT36644.1 .
    AB005234 Genomic DNA. Translation: BAB10454.1 .
    CP002688 Genomic DNA. Translation: AED97776.1 .
    BT005753 mRNA. Translation: AAO64158.1 .
    BT020376 mRNA. Translation: AAV85731.1 .
    AK228654 mRNA. Translation: BAF00561.1 .
    AJ439879 mRNA. Translation: CAD29165.1 . Frameshift.
    RefSeqi NP_201166.1. NM_125756.4.
    UniGenei At.28990.

    3D structure databases

    ProteinModelPortali Q84TI6.
    SMRi Q84TI6. Positions 13-413.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 21723. 22 interactions.
    IntActi Q84TI6. 4 interactions.

    Proteomic databases

    PaxDbi Q84TI6.
    PRIDEi Q84TI6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G63610.1 ; AT5G63610.1 ; AT5G63610 .
    GeneIDi 836481.
    KEGGi ath:AT5G63610.

    Organism-specific databases

    GeneFarmi 3295. 110.
    TAIRi AT5G63610.

    Phylogenomic databases

    eggNOGi COG0515.
    HOGENOMi HOG000233024.
    InParanoidi Q84TI6.
    KOi K02208.
    OMAi RSMPRPM.
    PhylomeDBi Q84TI6.

    Enzyme and pathway databases

    BioCyci ARA:AT5G63610-MONOMER.

    Gene expression databases

    Genevestigatori Q84TI6.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "HUA ENHANCER3 reveals a role for a cyclin-dependent protein kinase in the specification of floral organ identity in Arabidopsis."
      Wang W., Chen X.
      Development 131:3147-3156(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
    2. "Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence features of the 1.6 Mb regions covered by twenty physically assigned P1 clones."
      Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M., Miyajima N., Tabata S.
      DNA Res. 4:215-230(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Arabidopsis ORF clones."
      Cheuk R.F., Chen H., Kim C.J., Shinn P., Ecker J.R.
      Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    6. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
      Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
      , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
      Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    7. "The identification of Arabidopsis cyclin-dependent kinase 8."
      Pu S.Y., Huang H.J.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 170-470.
    8. "Genome-wide analysis of core cell cycle genes in Arabidopsis."
      Vandepoele K., Raes J., de Veylder L., Rouze P., Rombauts S., Inze D.
      Plant Cell 14:903-916(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.
    9. "Cell cycle regulation in plant development."
      Inze D., de Veylder L.
      Annu. Rev. Genet. 40:77-105(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW.
    10. "The transcription corepressor LEUNIG interacts with the histone deacetylase HDA19 and mediator components MED14 (SWP) and CDK8 (HEN3) to repress transcription."
      Gonzalez D., Bowen A.J., Carroll T.S., Conlan R.S.
      Mol. Cell. Biol. 27:5306-5315(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH MED14; LUG AND HDA19.

    Entry informationi

    Entry nameiCDKE1_ARATH
    AccessioniPrimary (citable) accession number: Q84TI6
    Secondary accession number(s): Q70Z09, Q9FFQ3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 10, 2007
    Last sequence update: July 10, 2007
    Last modified: October 1, 2014
    This is version 91 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3