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Q84TI6 (CDKE1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cyclin-dependent kinase E-1

Short name=CDKE;1
EC=2.7.11.22
EC=2.7.11.23
Alternative name(s):
Cyclin-dependent kinase 8
Protein HUA ENHANCER 3
Gene names
Name:CDKE-1
Synonyms:CDK8, HEN3
Ordered Locus Names:At5g63610
ORF Names:MBK5.8
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length470 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in cell differentiation. Required for the specification of stamen and carpel identities and for the proper termination of stem cells in the floral meristem. Ref.1

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

Subunit structure

Interacts with MED14, HDA19 and LUG. Ref.10

Subcellular location

Nucleus Probable.

Tissue specificity

Expressed in roots, leaves and stems. Expressed in young dividing tissue, such as shoot and root tips, lateral root primordia, young leaves and flowers. Expressed in the inflorescence meristem, inflorescence stem and young flowers. Ref.1

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

Contains 1 protein kinase domain.

Sequence caution

The sequence CAD29165.1 differs from that shown. Reason: Frameshift at position 401.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 470470Cyclin-dependent kinase E-1
PRO_0000293122

Regions

Domain25 – 333309Protein kinase
Nucleotide binding31 – 399ATP By similarity
Compositional bias445 – 4484Poly-Gln

Sites

Active site1541Proton acceptor By similarity
Binding site551ATP By similarity

Amino acid modifications

Modified residue361Phosphotyrosine By similarity

Experimental info

Sequence conflict4521K → E in AAO64158. Ref.4
Sequence conflict4521K → E in BAF00561. Ref.6

Sequences

Sequence LengthMass (Da)Tools
Q84TI6 [UniParc].

Last modified July 10, 2007. Version 2.
Checksum: 85A92D0000FD5E46

FASTA47052,797
        10         20         30         40         50         60 
MGDGSSSRSN SSNSTSEKPE WLQQYNLVGK IGEGTYGLVF LARTKTPPKR PIAIKKFKQS 

        70         80         90        100        110        120 
KDGDGVSPTA IREIMLLREI SHENVVKLVN VHINFADMSL YLAFDYAEYD LYEIIRHHRD 

       130        140        150        160        170        180 
KVGHSLNTYT VKSLLWQLLN GLNYLHSNWI IHRDLKPSNI LVMGDAEEHG IVKIADFGLA 

       190        200        210        220        230        240 
RIYQAPLKPL SDNGVVVTIW YRAPELLLGS KHYTSAVDMW AVGCIFAELL TLKPLFQGAE 

       250        260        270        280        290        300 
AKSSQNPFQL DQLDKIFKIL GHPTMDKWPT LVNLPHWQND VQHIQAHKYD SVGLHNVVHL 

       310        320        330        340        350        360 
NQKSPAYDLL SKMLEYDPLK RITASQALEH EYFRMDPLPG RNAFVASQPM EKNVNYPTRP 

       370        380        390        400        410        420 
VDTNTDFEGT TSINPPQAVA AGNVAGNMAG AHGMGSRSMP RPMVAHNMQR MQQSQGMMAY 

       430        440        450        460        470 
NFPAQAGLNP SVPLQQQRGM AQPHQQQQLR RKDPGMGMSG YAPPNKSRRL 

« Hide

References

« Hide 'large scale' references
[1]"HUA ENHANCER3 reveals a role for a cyclin-dependent protein kinase in the specification of floral organ identity in Arabidopsis."
Wang W., Chen X.
Development 131:3147-3156(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
[2]"Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence features of the 1.6 Mb regions covered by twenty physically assigned P1 clones."
Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M., Miyajima N., Tabata S.
DNA Res. 4:215-230(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Arabidopsis ORF clones."
Cheuk R.F., Chen H., Kim C.J., Shinn P., Ecker J.R.
Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[6]"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. expand/collapse author list , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[7]"The identification of Arabidopsis cyclin-dependent kinase 8."
Pu S.Y., Huang H.J.
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 170-470.
[8]"Genome-wide analysis of core cell cycle genes in Arabidopsis."
Vandepoele K., Raes J., de Veylder L., Rouze P., Rombauts S., Inze D.
Plant Cell 14:903-916(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
[9]"Cell cycle regulation in plant development."
Inze D., de Veylder L.
Annu. Rev. Genet. 40:77-105(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.
[10]"The transcription corepressor LEUNIG interacts with the histone deacetylase HDA19 and mediator components MED14 (SWP) and CDK8 (HEN3) to repress transcription."
Gonzalez D., Bowen A.J., Carroll T.S., Conlan R.S.
Mol. Cell. Biol. 27:5306-5315(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH MED14; LUG AND HDA19.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY600243 mRNA. Translation: AAT36644.1.
AB005234 Genomic DNA. Translation: BAB10454.1.
CP002688 Genomic DNA. Translation: AED97776.1.
BT005753 mRNA. Translation: AAO64158.1.
BT020376 mRNA. Translation: AAV85731.1.
AK228654 mRNA. Translation: BAF00561.1.
AJ439879 mRNA. Translation: CAD29165.1. Frameshift.
RefSeqNP_201166.1. NM_125756.4.
UniGeneAt.28990.

3D structure databases

ProteinModelPortalQ84TI6.
SMRQ84TI6. Positions 13-413.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid21723. 22 interactions.
IntActQ84TI6. 4 interactions.

Proteomic databases

PaxDbQ84TI6.
PRIDEQ84TI6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G63610.1; AT5G63610.1; AT5G63610.
GeneID836481.
KEGGath:AT5G63610.

Organism-specific databases

GeneFarm3295. 110.
TAIRAT5G63610.

Phylogenomic databases

eggNOGCOG0515.
HOGENOMHOG000233024.
InParanoidQ84TI6.
KOK02208.
OMARSMPRPM.
PhylomeDBQ84TI6.

Enzyme and pathway databases

BioCycARA:AT5G63610-MONOMER.

Gene expression databases

GenevestigatorQ84TI6.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCDKE1_ARATH
AccessionPrimary (citable) accession number: Q84TI6
Secondary accession number(s): Q70Z09, Q9FFQ3
Entry history
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: July 10, 2007
Last modified: June 11, 2014
This is version 90 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names