Q84TI6 (CDKE1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cyclin-dependent kinase E-1
Short name=CDKE;1
EC=2.7.11.22
EC=2.7.11.23

Alternative name(s):
Cyclin-dependent kinase 8
Protein HUA ENHANCER 3

Gene names

Name:	CDKE-1
Synonyms:	CDK8, HEN3
Ordered Locus Names:	At5g63610
ORF Names:	MBK5.8

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	470 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in cell differentiation. Required for the specification of stamen and carpel identities and for the proper termination of stem cells in the floral meristem. Ref.1
Catalytic activity	ATP + a protein = ADP + a phosphoprotein. ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.
Subunit structure	Interacts with MED14, HDA19 and LUG. Ref.10
Subcellular location	Nucleus Probable.
Tissue specificity	Expressed in roots, leaves and stems. Expressed in young dividing tissue, such as shoot and root tips, lateral root primordia, young leaves and flowers. Expressed in the inflorescence meristem, inflorescence stem and young flowers. Ref.1
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain.
Sequence caution	The sequence CAD29165.1 differs from that shown. Reason: Frameshift at position 401.

Ontologies

Keywords
Biological process	Cell cycle Cell division
Cellular component	Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell division Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA polymerase II carboxy-terminal domain kinase activity Inferred from electronic annotation. Source: EC cyclin-dependent protein serine/threonine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 470	470	Cyclin-dependent kinase E-1		PRO_0000293122
Regions
	Domain	25 – 333	309	Protein kinase
	Nucleotide binding	31 – 39	9	ATP By similarity
	Compositional bias	445 – 448	4	Poly-Gln
Sites
	Active site	154	1	Proton acceptor By similarity
	Binding site	55	1	ATP By similarity
Amino acid modifications
	Modified residue	35	1	Phosphothreonine By similarity
	Modified residue	36	1	Phosphotyrosine By similarity
Experimental info
	Sequence conflict	452	1	K → E in AAO64158. Ref.4
	Sequence conflict	452	1	K → E in BAF00561. Ref.6

Sequences

	Sequence		Length	Mass (Da)
	Q84TI6 [UniParc]. Last modified July 10, 2007. Version 2. Checksum: 85A92D0000FD5E46	FASTA	470	52,797
	10 20 30 40 50 60 MGDGSSSRSN SSNSTSEKPE WLQQYNLVGK IGEGTYGLVF LARTKTPPKR PIAIKKFKQS 70 80 90 100 110 120 KDGDGVSPTA IREIMLLREI SHENVVKLVN VHINFADMSL YLAFDYAEYD LYEIIRHHRD 130 140 150 160 170 180 KVGHSLNTYT VKSLLWQLLN GLNYLHSNWI IHRDLKPSNI LVMGDAEEHG IVKIADFGLA 190 200 210 220 230 240 RIYQAPLKPL SDNGVVVTIW YRAPELLLGS KHYTSAVDMW AVGCIFAELL TLKPLFQGAE 250 260 270 280 290 300 AKSSQNPFQL DQLDKIFKIL GHPTMDKWPT LVNLPHWQND VQHIQAHKYD SVGLHNVVHL 310 320 330 340 350 360 NQKSPAYDLL SKMLEYDPLK RITASQALEH EYFRMDPLPG RNAFVASQPM EKNVNYPTRP 370 380 390 400 410 420 VDTNTDFEGT TSINPPQAVA AGNVAGNMAG AHGMGSRSMP RPMVAHNMQR MQQSQGMMAY 430 440 450 460 470 NFPAQAGLNP SVPLQQQRGM AQPHQQQQLR RKDPGMGMSG YAPPNKSRRL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"HUA ENHANCER3 reveals a role for a cyclin-dependent protein kinase in the specification of floral organ identity in Arabidopsis." Wang W., Chen X. Development 131:3147-3156(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
[2]	"Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence features of the 1.6 Mb regions covered by twenty physically assigned P1 clones." Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M., Miyajima N., Tabata S. DNA Res. 4:215-230(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Arabidopsis ORF clones." Cheuk R.F., Chen H., Kim C.J., Shinn P., Ecker J.R. Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[6]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[7]	"The identification of Arabidopsis cyclin-dependent kinase 8." Pu S.Y., Huang H.J. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 170-470.
[8]	"Genome-wide analysis of core cell cycle genes in Arabidopsis." Vandepoele K., Raes J., de Veylder L., Rouze P., Rombauts S., Inze D. Plant Cell 14:903-916(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
[9]	"Cell cycle regulation in plant development." Inze D., de Veylder L. Annu. Rev. Genet. 40:77-105(2006) [PubMed] [Europe PMC] [Abstract] Cited for: REVIEW.
[10]	"The transcription corepressor LEUNIG interacts with the histone deacetylase HDA19 and mediator components MED14 (SWP) and CDK8 (HEN3) to repress transcription." Gonzalez D., Bowen A.J., Carroll T.S., Conlan R.S. Mol. Cell. Biol. 27:5306-5315(2007) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH MED14; LUG AND HDA19.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY600243 mRNA. Translation: AAT36644.1. AB005234 Genomic DNA. Translation: BAB10454.1. CP002688 Genomic DNA. Translation: AED97776.1. BT005753 mRNA. Translation: AAO64158.1. BT020376 mRNA. Translation: AAV85731.1. AK228654 mRNA. Translation: BAF00561.1. AJ439879 mRNA. Translation: CAD29165.1. Frameshift.
IPI	IPI00542328.
RefSeq	NP_201166.1. NM_125756.4.
UniGene	At.28990.
3D structure databases
ProteinModelPortal	Q84TI6.
SMR	Q84TI6. Positions 31-362.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q84TI6. 4 interactions.
Proteomic databases
PaxDb	Q84TI6.
PRIDE	Q84TI6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G63610.1; AT5G63610.1; AT5G63610.
GeneID	836481.
KEGG	ath:AT5G63610.
Organism-specific databases
GeneFarm	3295. 110.
TAIR	At5g63610.
Phylogenomic databases
eggNOG	COG0515.
HOGENOM	HOG000233024.
InParanoid	Q84TI6.
KO	K02208.
OMA	ADMSLYL.
PhylomeDB	Q84TI6.
ProtClustDB	CLSN2687570.
Gene expression databases
ArrayExpress	Q84TI6.
Genevestigator	Q84TI6.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CDKE1_ARATH

Accession

Primary (citable) accession number: Q84TI6
Secondary accession number(s): Q70Z09, Q9FFQ3

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	July 10, 2007
Last modified:	May 29, 2013
This is version 80 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program