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Protein

NAC domain-containing protein 21/22

Gene

NAC021

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional activator that mediates auxin signaling to promote lateral root development. Activates the expression of two downstream auxin-responsive genes, DBP and AIR3.1 Publication

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • transcription factor activity, sequence-specific DNA binding Source: TAIR

GO - Biological processi

  • auxin-activated signaling pathway Source: TAIR
  • lateral root development Source: TAIR
  • primary shoot apical meristem specification Source: TAIR
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
NAC domain-containing protein 21/22
Short name:
ANAC021/ANAC022
Gene namesi
Name:NAC021
Synonyms:NAC022, NAC1
Ordered Locus Names:At1g56010
ORF Names:F14J16.32, T6H22.19
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G56010.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 324324NAC domain-containing protein 21/22PRO_0000132310Add
BLAST

Post-translational modificationi

Ubiquitinated (Probable). The interaction with SINAT5 mediate its proteasome-dependent degradation.Curated

Keywords - PTMi

Ubl conjugation

Proteomic databases

PaxDbiQ84TE6.

Expressioni

Tissue specificityi

Predominantly expressed in the root tip and in lateral root initiation sites. Also detected in expanding cotyledon, and in leaf primordia.1 Publication

Inductioni

Induced by auxin.

Gene expression databases

ExpressionAtlasiQ84TE6. baseline and differential.
GenevisibleiQ84TE6. AT.

Interactioni

Subunit structurei

Dimer. Interacts with SINAT5.1 Publication

Protein-protein interaction databases

BioGridi27277. 2 interactions.
STRINGi3702.AT1G56010.2.

Structurei

3D structure databases

ProteinModelPortaliQ84TE6.
SMRiQ84TE6. Positions 16-171.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini19 – 171153NACPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi120 – 13718Bipartite nuclear localization signalSequence analysisAdd
BLAST

Domaini

The NAC domain includes a DNA-binding domain and a dimerization domain.

Sequence similaritiesi

Contains 1 NAC domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IIHI. Eukaryota.
ENOG410YDRK. LUCA.
HOGENOMiHOG000237406.
InParanoidiQ84TE6.
OMAiEPWDIPK.
PhylomeDBiQ84TE6.

Family and domain databases

InterProiIPR003441. NAC-dom.
[Graphical view]
PfamiPF02365. NAM. 1 hit.
[Graphical view]
SUPFAMiSSF101941. SSF101941. 1 hit.
PROSITEiPS51005. NAC. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q84TE6-1) [UniParc]FASTAAdd to basket

Also known as: ANAC022

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
METEEEMKES SISMVEAKLP PGFRFHPKDD ELVCDYLMRR SLHNNHRPPL
60 70 80 90 100
VLIQVDLNKC EPWDIPKMAC VGGKDWYFYS QRDRKYATGL RTNRATATGY
110 120 130 140 150
WKATGKDRTI LRKGKLVGMR KTLVFYQGRA PRGRKTDWVM HEFRLQGSHH
160 170 180 190 200
PPNHSLSSPK EDWVLCRVFH KNTEGVICRD NMGSCFDETA SASLPPLMDP
210 220 230 240 250
YINFDQEPSS YLSDDHHYII NEHVPCFSNL SQNQTLNSNL TNSVSELKIP
260 270 280 290 300
CKNPNPLFTG GSASATLTGL DSFCSSDQMV LRALLSQLTK IDGSLGPKES
310 320
QSYGEGSSES LLTDIGIPST VWNC
Length:324
Mass (Da):36,569
Last modified:August 16, 2004 - v2
Checksum:iC70ED705D1A06957
GO
Isoform 2 (identifier: Q84TE6-2) [UniParc]FASTAAdd to basket

Also known as: ANAC021

The sequence of this isoform differs from the canonical sequence as follows:
     1-67: Missing.

Show »
Length:257
Mass (Da):28,653
Checksum:iF95C6AB206618253
GO

Sequence cautioni

The sequence AAF02847.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 6767Missing in isoform 2. 2 PublicationsVSP_011189Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF198054 mRNA. Translation: AAF21437.1.
AC002304 Genomic DNA. Translation: AAF79328.1.
AC009894 Genomic DNA. Translation: AAF02847.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE33330.1.
CP002684 Genomic DNA. Translation: AEE33331.1.
BT005873 mRNA. Translation: AAO64808.1.
AK228347 mRNA. Translation: BAF00287.1.
AY085996 mRNA. Translation: AAM63206.1.
PIRiC96601.
RefSeqiNP_175997.1. NM_104479.2. [Q84TE6-1]
NP_849817.1. NM_179486.1. [Q84TE6-2]
UniGeneiAt.48312.
At.71152.

Genome annotation databases

EnsemblPlantsiAT1G56010.2; AT1G56010.2; AT1G56010. [Q84TE6-1]
GeneIDi842052.
KEGGiath:AT1G56010.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF198054 mRNA. Translation: AAF21437.1.
AC002304 Genomic DNA. Translation: AAF79328.1.
AC009894 Genomic DNA. Translation: AAF02847.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE33330.1.
CP002684 Genomic DNA. Translation: AEE33331.1.
BT005873 mRNA. Translation: AAO64808.1.
AK228347 mRNA. Translation: BAF00287.1.
AY085996 mRNA. Translation: AAM63206.1.
PIRiC96601.
RefSeqiNP_175997.1. NM_104479.2. [Q84TE6-1]
NP_849817.1. NM_179486.1. [Q84TE6-2]
UniGeneiAt.48312.
At.71152.

3D structure databases

ProteinModelPortaliQ84TE6.
SMRiQ84TE6. Positions 16-171.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi27277. 2 interactions.
STRINGi3702.AT1G56010.2.

Proteomic databases

PaxDbiQ84TE6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G56010.2; AT1G56010.2; AT1G56010. [Q84TE6-1]
GeneIDi842052.
KEGGiath:AT1G56010.

Organism-specific databases

TAIRiAT1G56010.

Phylogenomic databases

eggNOGiENOG410IIHI. Eukaryota.
ENOG410YDRK. LUCA.
HOGENOMiHOG000237406.
InParanoidiQ84TE6.
OMAiEPWDIPK.
PhylomeDBiQ84TE6.

Miscellaneous databases

PROiQ84TE6.

Gene expression databases

ExpressionAtlasiQ84TE6. baseline and differential.
GenevisibleiQ84TE6. AT.

Family and domain databases

InterProiIPR003441. NAC-dom.
[Graphical view]
PfamiPF02365. NAM. 1 hit.
[Graphical view]
SUPFAMiSSF101941. SSF101941. 1 hit.
PROSITEiPS51005. NAC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Arabidopsis NAC1 transduces auxin signal downstream of TIR1 to promote lateral root development."
    Xie Q., Frugis G., Colgan D.F., Chua N.-H.
    Genes Dev. 14:3024-3036(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, TISSUE SPECIFICITY, INDUCTION (ISOFORM 1).
    Strain: cv. Landsberg erecta.
  2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: cv. Columbia.
  5. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: cv. Columbia.
  6. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  7. "SINAT5 promotes ubiquitin-related degradation of NAC1 to attenuate auxin signals."
    Xie Q., Guo H.-S., Dallman G., Fang S., Weissman A.M., Chua N.-H.
    Nature 419:167-170(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEGRADATION, INTERACTION WITH SINAT5.
  8. Cited for: GENE FAMILY, NOMENCLATURE.

Entry informationi

Entry nameiNAC22_ARATH
AccessioniPrimary (citable) accession number: Q84TE6
Secondary accession number(s): Q0WRG2, Q9SE10, Q9SGS9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: August 16, 2004
Last modified: February 17, 2016
This is version 97 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.