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Q84T94

- P2C35_ORYSJ

UniProt

Q84T94 - P2C35_ORYSJ

Protein

Protein phosphatase 2C 35

Gene

XB15

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 72 (01 Oct 2014)
      Sequence version 1 (01 Jun 2003)
      Previous versions | rss
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    Functioni

    Protein phosphatase that acts on XA21 pathogen recognition receptor. Negatively regulates cell death and XA21-mediated innate immunity.1 Publication

    Catalytic activityi

    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

    Cofactori

    Binds 2 magnesium or manganese ions per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi262 – 2621Manganese 1By similarity
    Metal bindingi262 – 2621Manganese 2By similarity
    Metal bindingi263 – 2631Manganese 1; via carbonyl oxygenBy similarity
    Metal bindingi558 – 5581Manganese 2By similarity
    Metal bindingi621 – 6211Manganese 2By similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. phosphoprotein phosphatase activity Source: UniProtKB-KW

    GO - Biological processi

    1. defense response Source: UniProtKB-KW
    2. leaf development Source: EnsemblPlants/Gramene

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Biological processi

    Plant defense

    Keywords - Ligandi

    Magnesium, Manganese, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein phosphatase 2C 35 (EC:3.1.3.16)
    Short name:
    OsPP2C35
    Alternative name(s):
    XA21-binding protein 15
    Gene namesi
    Name:XB15
    Ordered Locus Names:Os03g0821300, LOC_Os03g60650
    ORF Names:OJ1754_E06.28, OsJ_012575
    OrganismiOryza sativa subsp. japonica (Rice)
    Taxonomic identifieri39947 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
    ProteomesiUP000000763: Chromosome 3

    Organism-specific databases

    GrameneiQ84T94.

    Subcellular locationi

    Cell membrane 1 Publication; Peripheral membrane protein 1 Publication; Cytoplasmic side 1 Publication
    Note: Associated with XA21 receptor kinase.

    GO - Cellular componenti

    1. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Enhanced resistance to Xantomonas oryzae pv. oryzae (Xoo), expression of defense-related genes and cell death (necrotic lesions).1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 639639Protein phosphatase 2C 35PRO_0000363282Add
    BLAST

    Proteomic databases

    PRIDEiQ84T94.

    Interactioni

    Subunit structurei

    Interacts with XA21 (via juxtamembrane and kinase domains).1 Publication

    Protein-protein interaction databases

    DIPiDIP-46055N.

    Structurei

    3D structure databases

    ProteinModelPortaliQ84T94.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini262 – 606345PP2C-likeAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi60 – 689Poly-Ala
    Compositional biasi218 – 2258Poly-Ala

    Sequence similaritiesi

    Belongs to the PP2C family.Curated
    Contains 1 PP2C-like domain.Curated

    Phylogenomic databases

    eggNOGiCOG0631.
    HOGENOMiHOG000239375.
    OMAiINEETMM.

    Family and domain databases

    Gene3Di3.60.40.10. 2 hits.
    InterProiIPR001932. PP2C-like_dom.
    IPR015655. Protein_Pase_2C.
    [Graphical view]
    PANTHERiPTHR13832. PTHR13832. 1 hit.
    PfamiPF00481. PP2C. 1 hit.
    [Graphical view]
    SMARTiSM00332. PP2Cc. 1 hit.
    [Graphical view]
    SUPFAMiSSF81606. SSF81606. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    Q84T94-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGNSLACFCC GGGAGGRGGR HVAPAALPSD PAYDEGLGHS FCYVRPDKFV    50
    VPFSADDLVA DAKAAAAAEG EATTFRAISG AALSANVSTP LSTSVLLLMP 100
    EESSASATAS SGFESSESFA AVPLQPVPRF SSGPISAPFS GGFMSGPLER 150
    GFQSGPLDAA LLSGPLPGTA TSGRMGGAVP ALRRSLSHGG RRLRNFTRAL 200
    LARTEKFQDS ADLGSPDAAA AAVAACGGDP CGLQWAQGKA GEDRVHVVVS 250
    EERGWVFVGI YDGFNGPDAT DFLVSNLYAA VHRELRGLLW DQREQNVQHD 300
    QRPDQPGSAP STTASDNQDQ WGRRRRTRRS RPPRGADDDQ RRWKCEWEQE 350
    RDCSNLKPPT QQRLRCNSEN DHVAVLKALT RALHRTEEAY LDIADKMVGE 400
    FPELALMGSC VLAMLMKGED MYIMNVGDSR AVLATMDSVD LEQISQGSFD 450
    GSVGDCPPCL SAVQLTSDHS TSVEEEVIRI RNEHPDDPSA ISKDRVKGSL 500
    KVTRAFGAGF LKQPKWNDAL LEMFRIDYVG SSPYISCNPS LFHHKLSTRD 550
    RFLILSSDGL YQYFTNEEAV AQVEMFIATT PEGDPAQHLV EEVLFRAANK 600
    AGMDFHELIE IPHGDRRRYH DDVSVIVISL EGRIWRSCV 639
    Length:639
    Mass (Da):69,180
    Last modified:June 1, 2003 - v1
    Checksum:iE64A0AED1838C00F
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti12 – 132GG → CC in EAZ29092. (PubMed:15685292)Curated
    Sequence conflicti23 – 231A → V in AK071722. (PubMed:12869764)Curated
    Sequence conflicti402 – 4021P → T in AK071722. (PubMed:12869764)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC104433 Genomic DNA. Translation: AAO65883.1.
    DP000009 Genomic DNA. Translation: ABF99594.1.
    AP008209 Genomic DNA. Translation: BAF13640.1.
    CM000140 Genomic DNA. Translation: EAZ29092.1.
    AK071722 mRNA. No translation available.
    AJ575237 mRNA. Translation: CAE00873.1.
    RefSeqiNP_001051726.1. NM_001058261.1.
    UniGeneiOs.19321.

    Genome annotation databases

    EnsemblPlantsiOS03T0821300-01; OS03T0821300-01; OS03G0821300.
    GeneIDi4334600.
    KEGGiosa:4334600.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC104433 Genomic DNA. Translation: AAO65883.1 .
    DP000009 Genomic DNA. Translation: ABF99594.1 .
    AP008209 Genomic DNA. Translation: BAF13640.1 .
    CM000140 Genomic DNA. Translation: EAZ29092.1 .
    AK071722 mRNA. No translation available.
    AJ575237 mRNA. Translation: CAE00873.1 .
    RefSeqi NP_001051726.1. NM_001058261.1.
    UniGenei Os.19321.

    3D structure databases

    ProteinModelPortali Q84T94.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-46055N.

    Proteomic databases

    PRIDEi Q84T94.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi OS03T0821300-01 ; OS03T0821300-01 ; OS03G0821300 .
    GeneIDi 4334600.
    KEGGi osa:4334600.

    Organism-specific databases

    Gramenei Q84T94.

    Phylogenomic databases

    eggNOGi COG0631.
    HOGENOMi HOG000239375.
    OMAi INEETMM.

    Family and domain databases

    Gene3Di 3.60.40.10. 2 hits.
    InterProi IPR001932. PP2C-like_dom.
    IPR015655. Protein_Pase_2C.
    [Graphical view ]
    PANTHERi PTHR13832. PTHR13832. 1 hit.
    Pfami PF00481. PP2C. 1 hit.
    [Graphical view ]
    SMARTi SM00332. PP2Cc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF81606. SSF81606. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. "Sequence, annotation, and analysis of synteny between rice chromosome 3 and diverged grass species."
      The rice chromosome 3 sequencing consortium
      Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B., Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T., Fadrosh D., Bera J., Weaver B., Jin S.
      , Johri S., Reardon M., Webb K., Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T., Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R., Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J., Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S., Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M., Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M., Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L., O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R., Jin W., Lee H.R., Jiang J., Jackson S.
      Genome Res. 15:1284-1291(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.
    3. "The rice annotation project database (RAP-DB): 2008 update."
      The rice annotation project (RAP)
      Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENOME REANNOTATION.
      Strain: cv. Nipponbare.
    4. "The genomes of Oryza sativa: a history of duplications."
      Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.
      , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.
      PLoS Biol. 3:266-281(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.
    5. "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
      The rice full-length cDNA consortium
      Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Nipponbare.
    6. "Development of an efficient method for the isolation of factors involved in gene transcription during rice embryo development."
      Ye R., Yao Q.-H., Xu Z.-H., Xue H.-W.
      Plant J. 38:348-357(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 486-639.
      Tissue: Embryo.
    7. "Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis."
      Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y.
      BMC Genomics 9:550-550(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.
    8. "Rice XB15, a protein phosphatase 2C, negatively regulates cell death and XA21-mediated innate immunity."
      Park C.-J., Peng Y., Chen X., Dardick C., Ruan D., Bart R., Canlas P.E., Ronald P.C.
      PLoS Biol. 6:E231-E231(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE, INTERACTION WITH XA21.

    Entry informationi

    Entry nameiP2C35_ORYSJ
    AccessioniPrimary (citable) accession number: Q84T94
    Secondary accession number(s): A3AP53, Q70KS9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 10, 2009
    Last sequence update: June 1, 2003
    Last modified: October 1, 2014
    This is version 72 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Oryza sativa (rice)
      Index of Oryza sativa entries and their corresponding gene designations
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3