Q84T94 (P2C35_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein phosphatase 2C 35
Short name=OsPP2C35
EC=3.1.3.16

Alternative name(s):
XA21-binding protein 15

Gene names

Name:	XB15
Ordered Locus Names:	Os03g0821300, LOC_Os03g60650
ORF Names:	OJ1754_E06.28, OsJ_012575

Organism

Oryza sativa subsp. japonica (Rice) [Reference proteome]

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza › Oryza sativa

Protein attributes

Sequence length	639 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Protein phosphatase that acts on XA21 pathogen recognition receptor. Negatively regulates cell death and XA21-mediated innate immunity. Ref.8
Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Subunit structure	Interacts with XA21 (via juxtamembrane and kinase domains). Ref.8
Subcellular location	Cell membrane; Peripheral membrane protein; Cytoplasmic side. Note: Associated with XA21 receptor kinase. Ref.8
Disruption phenotype	Enhanced resistance to Xantomonas oryzae pv. oryzae (Xoo), expression of defense-related genes and cell death (necrotic lesions). Ref.8
Sequence similarities	Belongs to the PP2C family. Contains 1 PP2C-like domain.

Ontologies

Keywords
Biological process	Plant defense
Cellular component	Cell membrane Membrane
Ligand	Magnesium Manganese Metal-binding
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	defense response Inferred from electronic annotation. Source: UniProtKB-KW leaf development Inferred from electronic annotation. Source: EnsemblPlants/Gramene metabolic process Inferred from electronic annotation. Source: GOC
Cellular_component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 639	639	Protein phosphatase 2C 35		PRO_0000363282
Regions
	Domain	262 – 606	345	PP2C-like
	Compositional bias	60 – 68	9	Poly-Ala
	Compositional bias	218 – 225	8	Poly-Ala
Sites
	Metal binding	262	1	Manganese 1 By similarity
	Metal binding	262	1	Manganese 2 By similarity
	Metal binding	263	1	Manganese 1; via carbonyl oxygen By similarity
	Metal binding	558	1	Manganese 2 By similarity
	Metal binding	621	1	Manganese 2 By similarity
Experimental info
	Sequence conflict	12 – 13	2	GG → CC in EAZ29092. Ref.4
	Sequence conflict	23	1	A → V in AK071722. Ref.5
	Sequence conflict	402	1	P → T in AK071722. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Q84T94 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: E64A0AED1838C00F	FASTA	639	69,180
	10 20 30 40 50 60 MGNSLACFCC GGGAGGRGGR HVAPAALPSD PAYDEGLGHS FCYVRPDKFV VPFSADDLVA 70 80 90 100 110 120 DAKAAAAAEG EATTFRAISG AALSANVSTP LSTSVLLLMP EESSASATAS SGFESSESFA 130 140 150 160 170 180 AVPLQPVPRF SSGPISAPFS GGFMSGPLER GFQSGPLDAA LLSGPLPGTA TSGRMGGAVP 190 200 210 220 230 240 ALRRSLSHGG RRLRNFTRAL LARTEKFQDS ADLGSPDAAA AAVAACGGDP CGLQWAQGKA 250 260 270 280 290 300 GEDRVHVVVS EERGWVFVGI YDGFNGPDAT DFLVSNLYAA VHRELRGLLW DQREQNVQHD 310 320 330 340 350 360 QRPDQPGSAP STTASDNQDQ WGRRRRTRRS RPPRGADDDQ RRWKCEWEQE RDCSNLKPPT 370 380 390 400 410 420 QQRLRCNSEN DHVAVLKALT RALHRTEEAY LDIADKMVGE FPELALMGSC VLAMLMKGED 430 440 450 460 470 480 MYIMNVGDSR AVLATMDSVD LEQISQGSFD GSVGDCPPCL SAVQLTSDHS TSVEEEVIRI 490 500 510 520 530 540 RNEHPDDPSA ISKDRVKGSL KVTRAFGAGF LKQPKWNDAL LEMFRIDYVG SSPYISCNPS 550 560 570 580 590 600 LFHHKLSTRD RFLILSSDGL YQYFTNEEAV AQVEMFIATT PEGDPAQHLV EEVLFRAANK 610 620 630 AGMDFHELIE IPHGDRRRYH DDVSVIVISL EGRIWRSCV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence, annotation, and analysis of synteny between rice chromosome 3 and diverged grass species." The rice chromosome 3 sequencing consortium Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B., Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T., Fadrosh D., Bera J., Weaver B., Jin S. , Johri S., Reardon M., Webb K., Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T., Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R., Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J., Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S., Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M., Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M., Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L., O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R., Jin W., Lee H.R., Jiang J., Jackson S. Genome Res. 15:1284-1291(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"The rice annotation project database (RAP-DB): 2008 update." The rice annotation project (RAP) Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[4]	"The genomes of Oryza sativa: a history of duplications." Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H. PLoS Biol. 3:266-281(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[5]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.
[6]	"Development of an efficient method for the isolation of factors involved in gene transcription during rice embryo development." Ye R., Yao Q.-H., Xu Z.-H., Xue H.-W. Plant J. 38:348-357(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 486-639. Tissue: Embryo.
[7]	"Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis." Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y. BMC Genomics 9:550-550(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
[8]	"Rice XB15, a protein phosphatase 2C, negatively regulates cell death and XA21-mediated innate immunity." Park C.-J., Peng Y., Chen X., Dardick C., Ruan D., Bart R., Canlas P.E., Ronald P.C. PLoS Biol. 6:E231-E231(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE, INTERACTION WITH XA21.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC104433 Genomic DNA. Translation: AAO65883.1. DP000009 Genomic DNA. Translation: ABF99594.1. AP008209 Genomic DNA. Translation: BAF13640.1. CM000140 Genomic DNA. Translation: EAZ29092.1. AK071722 mRNA. No translation available. AJ575237 mRNA. Translation: CAE00873.1.
RefSeq	NP_001051726.1. NM_001058261.1.
UniGene	Os.19321.
3D structure databases
ProteinModelPortal	Q84T94.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-46055N.
Proteomic databases
PRIDE	Q84T94.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	LOC_Os03g60650.1; LOC_Os03g60650.1; LOC_Os03g60650.
GeneID	4334600.
KEGG	dosa:Os03t0821300-01. osa:4334600.
Organism-specific databases
Gramene	Q84T94.
Phylogenomic databases
eggNOG	COG0631.
HOGENOM	HOG000239375.
OMA	TTPEGDP.
ProtClustDB	CLSN2694472.
Gene expression databases
ArrayExpress	Q84T94.
Family and domain databases
Gene3D	3.60.40.10. 2 hits.
InterPro	IPR001932. PP2C-like. IPR015655. Protein_Pase_2C. [Graphical view]
PANTHER	PTHR13832. PTHR13832. 1 hit.
Pfam	PF00481. PP2C. 1 hit. [Graphical view]
SMART	SM00332. PP2Cc. 1 hit. [Graphical view]
SUPFAM	SSF81606. PP2C-related. 1 hit.
PROSITE	PS01032. PP2C. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

P2C35_ORYSJ

Accession

Primary (citable) accession number: Q84T94
Secondary accession number(s): A3AP53, Q70KS9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 10, 2009
Last sequence update:	June 1, 2003
Last modified:	April 3, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program