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Q84T94 (P2C35_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein phosphatase 2C 35

Short name=OsPP2C35
EC=3.1.3.16
Alternative name(s):
XA21-binding protein 15
Gene names
Name:XB15
Ordered Locus Names:Os03g0821300, LOC_Os03g60650
ORF Names:OJ1754_E06.28, OsJ_012575
OrganismOryza sativa subsp. japonica (Rice) [Reference proteome]
Taxonomic identifier39947 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza

Protein attributes

Sequence length639 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Protein phosphatase that acts on XA21 pathogen recognition receptor. Negatively regulates cell death and XA21-mediated innate immunity. Ref.8

Catalytic activity

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Subunit structure

Interacts with XA21 (via juxtamembrane and kinase domains). Ref.8

Subcellular location

Cell membrane; Peripheral membrane protein; Cytoplasmic side. Note: Associated with XA21 receptor kinase. Ref.8

Disruption phenotype

Enhanced resistance to Xantomonas oryzae pv. oryzae (Xoo), expression of defense-related genes and cell death (necrotic lesions). Ref.8

Sequence similarities

Belongs to the PP2C family.

Contains 1 PP2C-like domain.

Ontologies

Keywords
   Biological processPlant defense
   Cellular componentCell membrane
Membrane
   LigandMagnesium
Manganese
Metal-binding
   Molecular functionHydrolase
Protein phosphatase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processdefense response

Inferred from electronic annotation. Source: UniProtKB-KW

leaf development

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

   Cellular_componentplasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionmetal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

phosphoprotein phosphatase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 639639Protein phosphatase 2C 35
PRO_0000363282

Regions

Domain262 – 606345PP2C-like
Compositional bias60 – 689Poly-Ala
Compositional bias218 – 2258Poly-Ala

Sites

Metal binding2621Manganese 1 By similarity
Metal binding2621Manganese 2 By similarity
Metal binding2631Manganese 1; via carbonyl oxygen By similarity
Metal binding5581Manganese 2 By similarity
Metal binding6211Manganese 2 By similarity

Experimental info

Sequence conflict12 – 132GG → CC in EAZ29092. Ref.4
Sequence conflict231A → V in AK071722. Ref.5
Sequence conflict4021P → T in AK071722. Ref.5

Sequences

Sequence LengthMass (Da)Tools
Q84T94 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: E64A0AED1838C00F

FASTA63969,180
        10         20         30         40         50         60 
MGNSLACFCC GGGAGGRGGR HVAPAALPSD PAYDEGLGHS FCYVRPDKFV VPFSADDLVA 

        70         80         90        100        110        120 
DAKAAAAAEG EATTFRAISG AALSANVSTP LSTSVLLLMP EESSASATAS SGFESSESFA 

       130        140        150        160        170        180 
AVPLQPVPRF SSGPISAPFS GGFMSGPLER GFQSGPLDAA LLSGPLPGTA TSGRMGGAVP 

       190        200        210        220        230        240 
ALRRSLSHGG RRLRNFTRAL LARTEKFQDS ADLGSPDAAA AAVAACGGDP CGLQWAQGKA 

       250        260        270        280        290        300 
GEDRVHVVVS EERGWVFVGI YDGFNGPDAT DFLVSNLYAA VHRELRGLLW DQREQNVQHD 

       310        320        330        340        350        360 
QRPDQPGSAP STTASDNQDQ WGRRRRTRRS RPPRGADDDQ RRWKCEWEQE RDCSNLKPPT 

       370        380        390        400        410        420 
QQRLRCNSEN DHVAVLKALT RALHRTEEAY LDIADKMVGE FPELALMGSC VLAMLMKGED 

       430        440        450        460        470        480 
MYIMNVGDSR AVLATMDSVD LEQISQGSFD GSVGDCPPCL SAVQLTSDHS TSVEEEVIRI 

       490        500        510        520        530        540 
RNEHPDDPSA ISKDRVKGSL KVTRAFGAGF LKQPKWNDAL LEMFRIDYVG SSPYISCNPS 

       550        560        570        580        590        600 
LFHHKLSTRD RFLILSSDGL YQYFTNEEAV AQVEMFIATT PEGDPAQHLV EEVLFRAANK 

       610        620        630 
AGMDFHELIE IPHGDRRRYH DDVSVIVISL EGRIWRSCV 

« Hide

References

« Hide 'large scale' references
[1]"Sequence, annotation, and analysis of synteny between rice chromosome 3 and diverged grass species."
The rice chromosome 3 sequencing consortium
Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B., Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T., Fadrosh D., Bera J., Weaver B., Jin S. expand/collapse author list , Johri S., Reardon M., Webb K., Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T., Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R., Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J., Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S., Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M., Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M., Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L., O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R., Jin W., Lee H.R., Jiang J., Jackson S.
Genome Res. 15:1284-1291(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[2]"The map-based sequence of the rice genome."
International rice genome sequencing project (IRGSP)
Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[3]"The rice annotation project database (RAP-DB): 2008 update."
The rice annotation project (RAP)
Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: cv. Nipponbare.
[4]"The genomes of Oryza sativa: a history of duplications."
Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. expand/collapse author list , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.
PLoS Biol. 3:266-281(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[5]"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
The rice full-length cDNA consortium
Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Nipponbare.
[6]"Development of an efficient method for the isolation of factors involved in gene transcription during rice embryo development."
Ye R., Yao Q.-H., Xu Z.-H., Xue H.-W.
Plant J. 38:348-357(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 486-639.
Tissue: Embryo.
[7]"Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis."
Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y.
BMC Genomics 9:550-550(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
[8]"Rice XB15, a protein phosphatase 2C, negatively regulates cell death and XA21-mediated innate immunity."
Park C.-J., Peng Y., Chen X., Dardick C., Ruan D., Bart R., Canlas P.E., Ronald P.C.
PLoS Biol. 6:E231-E231(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE, INTERACTION WITH XA21.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC104433 Genomic DNA. Translation: AAO65883.1.
DP000009 Genomic DNA. Translation: ABF99594.1.
AP008209 Genomic DNA. Translation: BAF13640.1.
CM000140 Genomic DNA. Translation: EAZ29092.1.
AK071722 mRNA. No translation available.
AJ575237 mRNA. Translation: CAE00873.1.
RefSeqNP_001051726.1. NM_001058261.1.
UniGeneOs.19321.

3D structure databases

ProteinModelPortalQ84T94.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-46055N.

Proteomic databases

PRIDEQ84T94.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsOS03T0821300-01; OS03T0821300-01; OS03G0821300.
GeneID4334600.
KEGGosa:4334600.

Organism-specific databases

GrameneQ84T94.

Phylogenomic databases

eggNOGCOG0631.
HOGENOMHOG000239375.
OMAINEETMM.
ProtClustDBCLSN2694472.

Family and domain databases

Gene3D3.60.40.10. 2 hits.
InterProIPR001932. PP2C-like_dom.
IPR015655. Protein_Pase_2C.
[Graphical view]
PANTHERPTHR13832. PTHR13832. 1 hit.
PfamPF00481. PP2C. 1 hit.
[Graphical view]
SMARTSM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMSSF81606. SSF81606. 2 hits.
ProtoNetSearch...

Entry information

Entry nameP2C35_ORYSJ
AccessionPrimary (citable) accession number: Q84T94
Secondary accession number(s): A3AP53, Q70KS9
Entry history
Integrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: June 1, 2003
Last modified: April 16, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Oryza sativa (rice)

Index of Oryza sativa entries and their corresponding gene designations