Q84T20 (Q84T20_HORVD) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Beta-amylase RuleBase RU000509
EC=3.2.1.2 RuleBase RU000509

Gene names

Name:	bamy1 EMBL AAO67355.1
Synonyms:	BAM1 EMBL CAX51376.1, bmy1 EMBL ACF05415.1

Organism

Hordeum vulgare var. distichum (Two-rowed barley) EMBL AAO67355.1

Taxonomic identifier

112509 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Pooideae › Triticeae › Hordeum

Protein attributes

Sequence length	535 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Hydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides so as to remove successive maltose units from the non-reducing ends of the chains. RuleBase RU000509
Sequence similarities	Belongs to the glycosyl hydrolase 14 family. RuleBase RU004157

Ontologies

Keywords
Biological process	Carbohydrate metabolism Polysaccharide degradation RuleBase RU000509
Molecular function	Glycosidase RuleBase RU000509 EMBL CAX51376.1 Hydrolase
Gene Ontology (GO)
Biological process	polysaccharide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	beta-amylase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	Q84T20 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 4653050B0F05F0A7	FASTA	535	59,572
	10 20 30 40 50 60 MEVNVKGNYV QVYVMLPLDA VSVNNRFEKG DELRAQLRKL VEAGVDGVMV DVWWGLVEGK 70 80 90 100 110 120 GPKAYDWSAY KQLFELVQKA GLKLQAIMSF HQCGGNVGDA VNIPIPQWVR DVGTCDPDIF 130 140 150 160 170 180 YTDGHGTRNI EYLTLGVDNQ PLFHGRSAVQ MYADYMTSFR ENMKEFLDAG VIVDIEVGLG 190 200 210 220 230 240 PAGEMRYPSY PQSHGWSFPG IGEFICYDKY LQADFKAAAA AVGHPEWEFP NDVGQYNDTP 250 260 270 280 290 300 ERTQFFRDNG TYLSEKGRFF LAWYSNNLIK HGDRILDEAN KVFLGYKVQL AIKISGIHWW 310 320 330 340 350 360 YKVPSHAAEL TAGYYNLHDR DGYRTIARML KRHRASINFT CAEMRDSEQS SQAMSAPEEL 370 380 390 400 410 420 VQQVLSAGWR EGLNVACENA LPRYDPTAYN TILRNARPHG INQSGPPEHK LFGFTYLRLS 430 440 450 460 470 480 NQLVEGQNYA NFKTFVDRMH ANLPRDPYVD PMAPLPRSGP EISIEMILQA AQPKLQPFPF 490 500 510 520 530 QEHTDLPVGP TGGMGGQAEG PTCGMGGQVK GPTGGMGGQA EDPTSGMGGE LPATM

References

[1]	"SPATIO-TEMPORAL PROFILING OF STARCH BIOSYNTHESIS AND DEGRADATION IN THE DEVELOPING BARLEY GRAIN." Radchuk V.V., Borisjuk L., Sreenivasulu N., Merx K., Mock H.P., Rolletschek H., Wobus U., Weschke W. Plant Physiol. 150:190-204(2009) Cited for: NUCLEOTIDE SEQUENCE.
[2]	"Differential RNA expression of Bmy1 during barley seed development and the association with ?-amylase accumulation, activity, and total protein." Vinje M.A., Willis D.K., Duke S.H., Henson C.A. Plant Physiol. Biochem. 49:39-45(2011) [PubMed: 20974538] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[3]	"Effects of single nucleotide polymorphisms in beta-amylase1 alleles from barley on functional properties of the enzymes." Clark S.E., Hayes P.M., Henson C.A. Plant Physiol. Biochem. 41:798-804(2003) Cited for: NUCLEOTIDE SEQUENCE.
[4]	"DNA variation in the exons and introns of Bmy1 does not account for phenotypic variation in diastatic power, beta amylase 1 enzyme thermostability, or enzyme activity in experimental barley germplasm." Filichkin T.P., Helgesson J., Hayes P.M., Tanaka Y., Duke S.H., Henson C.A. Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF414081 mRNA. Translation: AAO67355.1. EU589328 Genomic DNA. Translation: ACF05415.1. FJ161079 Genomic DNA. Translation: ACN24987.1. FN179393 mRNA. Translation: CAX51376.1.
UniGene	Hv.2064.
3D structure databases
HSSP	HSSP built from PDB template 1B1Y based on UniProtKB P16098.
ProteinModelPortal	Q84T20.
SMR	Q84T20. Positions 5-504.
ModBase	Search...
Protein family/group databases
CAZy	GH14. Glycoside Hydrolase Family 14.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
Gramene	Q84T20.
Family and domain databases
InterPro	IPR001554. Glyco_hydro_14. IPR018238. Glyco_hydro_14_CS. IPR001371. Glyco_hydro_14B_pln. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF01373. Glyco_hydro_14. 1 hit. [Graphical view]
PRINTS	PR00750. BETAAMYLASE. PR00842. GLHYDLASE14B.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00506. BETA_AMYLASE_1. 1 hit. PS00679. BETA_AMYLASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

Q84T20_HORVD

Accession

Primary (citable) accession number: Q84T20

Entry history

Integrated into UniProtKB/TrEMBL:	June 1, 2003
Last sequence update:	June 1, 2003
Last modified:	November 16, 2011
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)