Q84RQ8 (ARI15_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable E3 ubiquitin-protein ligase ARI15
EC=6.3.2.-

Alternative name(s):
ARIADNE-like protein ARI15
Protein ariadne homolog 15

Gene names

Name:	ARI15
Ordered Locus Names:	At5g63760
ORF Names:	MBK5.24

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	452 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Might act as an E3 ubiquitin-protein ligase, or as part of E3 complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes and then transfers it to substrates By similarity. Ref.5
Cofactor	Binds 4 zinc ions per subunit Potential.
Pathway	Protein modification; protein ubiquitination.
Tissue specificity	Ubiquitous. Ref.1
Domain	The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme By similarity.
Sequence similarities	Belongs to the RBR family. Ariadne subfamily. Contains 1 IBR-type zinc finger. Contains 1 RanBP2-type zinc finger. Contains 2 RING-type zinc fingers.
Caution	Lacks two Cys residues in the RING-type zinc finger domain 2 that are conserved features of the family.
Sequence caution	The sequence BAB10469.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Domain	Repeat Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein ubiquitination Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular_function	ligase activity Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 452	452	Probable E3 ubiquitin-protein ligase ARI15		PRO_0000356208
Regions
	Zinc finger	26 – 88	63	RING-type 1; atypical
	Zinc finger	106 – 174	69	IBR-type
	Zinc finger	208 – 239	32	RING-type 2; degenerate
	Zinc finger	414 – 445	32	RanBP2-type

Sequences

	Sequence		Length	Mass (Da)
	Q84RQ8 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: D2F3A23CC5DDEA20	FASTA	452	51,767
	10 20 30 40 50 60 MEKLMTESGL KPVVIDSNQD LSRVYCGICS NIGDDDYDGD AVVVDGDLIS TPFCSHKFCK 70 80 90 100 110 120 ACWSKYLKKN FFSVEKNHTA ISCPDRDCRA AVGPETVEKL TVRDQAMYEL YILKSYREKY 130 140 150 160 170 180 LGWKLKLCPA RGCNYVIEFH LASEDEEHSL NIVCLCGHIF CWRCMLESHR PVTCNNASDW 190 200 210 220 230 240 LSRDLEKLIE EVDKPSTVSW IDANTKPCPH CFIPVEIDGE RPWAQFLTCV CSGRFCWKCF 250 260 270 280 290 300 RSPETHGTSG SCLAPARSSN VGFNHWNRAK PGISCLDLWN ASQVNLVNAK YELEAFEESI 310 320 330 340 350 360 IKKPSDLKEQ DVKVLREGLM LIVQCRQFLK WSCAYDYIHT EYDMAKREYL RFLQQNASGI 370 380 390 400 410 420 VHSFSQSIKE ETEAKELTCG KLLSETTNIG NFFYHFIKTL REGLPEVQAE SYDNYGGPYW 430 440 450 LCDRCTYGNS WFQRACKMCC DPTASKMDEL SD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and characterization of the ARIADNE gene family in Arabidopsis. A group of putative E3 ligases." Mladek C., Guger K., Hauser M.-T. Plant Physiol. 131:27-40(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY, NOMENCLATURE, GENE FAMILY. Strain: cv. Columbia.
[2]	"Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence features of the 1.6 Mb regions covered by twenty physically assigned P1 clones." Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M., Miyajima N., Tabata S. DNA Res. 4:215-230(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Arabidopsis ORF clones." Quinitio C., Chen H., Kim C.J., Shinn P., Ecker J.R. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Comparative genomics of the RBR family, including the Parkinson's disease-related gene parkin and the genes of the ariadne subfamily." Marin I., Ferrus A. Mol. Biol. Evol. 19:2039-2050(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Web resources

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ510218 Genomic DNA. Translation: CAD52897.1. AB005234 Genomic DNA. Translation: BAB10469.1. Sequence problems. CP002688 Genomic DNA. Translation: AED97793.1. CP002688 Genomic DNA. Translation: AED97794.1. BT022004 mRNA. Translation: AAY25416.1. BT029215 mRNA. Translation: ABJ17150.1.
IPI	IPI00538933.
RefSeq	NP_201181.2. NM_125771.3. NP_974987.1. NM_203258.1.
UniGene	At.28980.
3D structure databases
HSSP	HSSP built from PDB template 1WD2 based on UniProtKB Q9Y4X5.
ProteinModelPortal	Q84RQ8.
SMR	Q84RQ8. Positions 22-97.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT5G63760.2-P.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G63760.1; AT5G63760.1; AT5G63760. AT5G63760.2; AT5G63760.2; AT5G63760.
GeneID	836496.
KEGG	ath:AT5G63760.
Organism-specific databases
TAIR	At5g63760.
Phylogenomic databases
eggNOG	NOG306332.
HOGENOM	HOG000034049.
InParanoid	Q84RQ8.
KO	K11968.
OMA	SETTNIG.
PhylomeDB	Q84RQ8.
ProtClustDB	CLSN2687579.
Enzyme and pathway databases
UniPathway	UPA00143.
Gene expression databases
Genevestigator	Q84RQ8.
Family and domain databases
Gene3D	3.30.40.10. 1 hit.
InterPro	IPR002867. Znf_C6HC. IPR001876. Znf_RanBP2. IPR001841. Znf_RING. IPR013083. Znf_RING/FYVE/PHD. [Graphical view]
Pfam	PF01485. IBR. 1 hit. [Graphical view]
SMART	SM00647. IBR. 2 hits. SM00184. RING. 2 hits. [Graphical view]
PROSITE	PS01358. ZF_RANBP2_1. 1 hit. PS50199. ZF_RANBP2_2. False negative. PS00518. ZF_RING_1. 1 hit. PS50089. ZF_RING_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ARI15_ARATH

Accession

Primary (citable) accession number: Q84RQ8
Secondary accession number(s): Q9FFN8

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 16, 2008
Last sequence update:	June 1, 2003
Last modified:	May 1, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program