Q84R49 (GUN10_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Endoglucanase 10
EC=3.2.1.4

Alternative name(s):
Endo-1,4-beta glucanase 10
OsGLU2

Gene names

Name:	GLU2
Ordered Locus Names:	Os03g0736300, LOC_Os03g52630
ORF Names:	OSJNBb0016H12.13, OsJ_12485

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	620 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Subcellular location	Membrane; Single-pass type II membrane protein Potential.
Tissue specificity	Ubiquitous. Ref.7
Sequence similarities	Belongs to the glycosyl hydrolase 9 (cellulase E) family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cell wall biogenesis/degradation Cellulose degradation Polysaccharide degradation
Cellular component	Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	cellulase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 620	620	Endoglucanase 10		PRO_0000249287
Regions
	Transmembrane	72 – 92	21	Helical; Signal-anchor for type II membrane protein; Potential
Sites
	Active site	513	1	By similarity
	Active site	561	1	By similarity
	Active site	570	1	By similarity
Amino acid modifications
	Glycosylation	216	1	N-linked (GlcNAc...) Potential
	Glycosylation	314	1	N-linked (GlcNAc...) Potential
	Glycosylation	323	1	N-linked (GlcNAc...) Potential
	Glycosylation	344	1	N-linked (GlcNAc...) Potential
	Glycosylation	408	1	N-linked (GlcNAc...) Potential
	Glycosylation	425	1	N-linked (GlcNAc...) Potential
	Glycosylation	567	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q84R49 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 2B5721EBD2ED7D52	FASTA	620	69,067
	10 20 30 40 50 60 MFGRDPWGGP LEISNADSAT DDDRSRDLDR GALMRQLDET QQSWLLAGPG DQAGKKKKKY 70 80 90 100 110 120 VDLGCMVLDR KIFMWTVGTI LGVGLFIGFV MMIVKLVPHK RPPPPPPDQY TQALHKALMF 130 140 150 160 170 180 FNAQRSGPLP KHNGVSWRGN SCMKDGLSDS TVRKSLVGGF YDAGDAIKFN YPMAWSMTML 190 200 210 220 230 240 SWSVIEYKAK YEAIGELDHV KELIKWGTDY LLKTFNSSAD TIDRIVAQVG VGDTSKGGAQ 250 260 270 280 290 300 PNDHYCWMRP EDIDYPRPVT ECHSCSDLAS EMAAALAAAS IVFKDSKTYS DKLVRGAKAL 310 320 330 340 350 360 YKFGRLQRGR YSPNGSDQAI FYNSTSYWDE FVWGGAWMYF ATGNNTYLSV ATAPGMAKHA 370 380 390 400 410 420 GAYWLDSPNY GVFTWDDKLP GAQVLLSRLR LFLSPGYPYE EILRTFHNQT DNVMCSYLPM 430 440 450 460 470 480 YNSFNFTKGG MIQLNHGRPQ PLQYVVNAAF LASLYSDYLD AADTPGWYCG PTFYTTEVLR 490 500 510 520 530 540 KFARSQLDYV LGKNPLKMSY VVGFGNKYPK RAHHRGASIP HNGVKYGCKG GFKWRETKKP 550 560 570 580 590 600 NPNILIGALV AGPDRHDGFK DVRTNYNYTE PTLAANAGLV AALISLTNIH VKSGIDKNTI 610 620 FSAVPPMFPT PPPPPSAWKP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A complex history of rearrangement in an orthologous region of the maize, sorghum, and rice genomes." Ilic K., SanMiguel P.J., Bennetzen J.L. Proc. Natl. Acad. Sci. U.S.A. 100:12265-12270(2003) [PubMed: 14530400] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequence, annotation, and analysis of synteny between rice chromosome 3 and diverged grass species." The rice chromosome 3 sequencing consortium Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B., Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T., Fadrosh D., Bera J., Weaver B., Jin S. , Johri S., Reardon M., Webb K., Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T., Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R., Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J., Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S., Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M., Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M., Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L., O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R., Jin W., Lee H.R., Jiang J., Jackson S. Genome Res. 15:1284-1291(2005) [PubMed: 16109971] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[4]	"The rice annotation project database (RAP-DB): 2008 update." The rice annotation project (RAP) Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed: 18089549] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[5]	"The genomes of Oryza sativa: a history of duplications." Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H. PLoS Biol. 3:266-281(2005) [PubMed: 15685292] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[6]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed: 12869764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.
[7]	"OsGLU1, a putative membrane-bound endo-1,4-beta-D-glucanase from rice, affects plant internode elongation." Zhou H.-L., He S.-J., Cao Y.-R., Chen T., Du B.-X., Chu C.-C., Zhang J.-S., Chen S.-Y. Plant Mol. Biol. 60:137-151(2006) [PubMed: 16463105] [Abstract] Cited for: TISSUE SPECIFICITY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY387483 Genomic DNA. Translation: AAR07086.1. AC118133 Genomic DNA. Translation: AAP03405.1. DP000009 Genomic DNA. Translation: ABF98746.1. AP008209 Genomic DNA. Translation: BAF13106.1. CM000140 Genomic DNA. Translation: EEE59884.1. AK070408 mRNA. Translation: BAG91933.1.
RefSeq	NP_001051192.1. NM_001057727.1.
UniGene	Os.59414.
3D structure databases
HSSP	HSSP built from PDB template 1TF4 based on UniProtKB P26221.
ProteinModelPortal	Q84R49.
ModBase	Search...
Protein-protein interaction databases
STRING	Q84R49.
Protein family/group databases
CAZy	GH9. Glycoside Hydrolase Family 9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	LOC_Os03g52630.1; LOC_Os03g52630.1; LOC_Os03g52630.
GeneID	4334029.
KEGG	osa:4334029.
Organism-specific databases
Gramene	Q84R49.
Phylogenomic databases
GeneTree	EPGT00070000028567.
HOGENOM	HBG746347.
OMA	ASIPHNG.
PhylomeDB	Q84R49.
ProtClustDB	CLSN2679649.
Family and domain databases
InterPro	IPR008928. 6-hairpin_glycosidase-like. IPR012341. 6hp_glycosidase. IPR001701. Glyco_hydro_9. IPR018221. Glyco_hydro_9_AS. [Graphical view]
Gene3D	G3DSA:1.50.10.10. CelA/Cel48F_cat. 1 hit.
KO	K01179.
Pfam	PF00759. Glyco_hydro_9. 1 hit. [Graphical view]
SUPFAM	SSF48208. Glyco_trans_6hp. 1 hit.
PROSITE	PS00592. GLYCOSYL_HYDROL_F9_1. False negative. PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUN10_ORYSJ

Accession

Primary (citable) accession number: Q84R49
Secondary accession number(s): Q0DNT2

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2006
Last sequence update:	June 1, 2003
Last modified:	December 14, 2011
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program