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Protein

Endoglucanase 10

Gene

GLU2

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei513 – 5131PROSITE-ProRule annotation
Active sitei561 – 5611PROSITE-ProRule annotation
Active sitei570 – 5701PROSITE-ProRule annotation

GO - Molecular functioni

  1. cellulase activity Source: UniProtKB-EC

GO - Biological processi

  1. cellulose catabolic process Source: UniProtKB-KW
  2. cell wall organization Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cell wall biogenesis/degradation, Cellulose degradation, Polysaccharide degradation

Protein family/group databases

CAZyiGH9. Glycoside Hydrolase Family 9.

Names & Taxonomyi

Protein namesi
Recommended name:
Endoglucanase 10 (EC:3.2.1.4)
Alternative name(s):
Endo-1,4-beta glucanase 10
OsGLU2
Gene namesi
Name:GLU2
Ordered Locus Names:Os03g0736300, LOC_Os03g52630
ORF Names:OsJ_12485, OSJNBb0016H12.13
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
ProteomesiUP000000763: Chromosome 3

Organism-specific databases

GrameneiQ84R49.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei72 – 9221Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 620620Endoglucanase 10PRO_0000249287Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi216 – 2161N-linked (GlcNAc...)Sequence Analysis
Glycosylationi314 – 3141N-linked (GlcNAc...)Sequence Analysis
Glycosylationi323 – 3231N-linked (GlcNAc...)Sequence Analysis
Glycosylationi344 – 3441N-linked (GlcNAc...)Sequence Analysis
Glycosylationi408 – 4081N-linked (GlcNAc...)Sequence Analysis
Glycosylationi425 – 4251N-linked (GlcNAc...)Sequence Analysis
Glycosylationi567 – 5671N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Expressioni

Tissue specificityi

Ubiquitous.1 Publication

Gene expression databases

ExpressionAtlasiQ84R49. baseline.

Interactioni

Protein-protein interaction databases

STRINGi39947.LOC_Os03g52630.1.

Structurei

3D structure databases

ProteinModelPortaliQ84R49.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG257354.
HOGENOMiHOG000021033.
InParanoidiQ84R49.
KOiK01179.
OMAiFIMMIVK.

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR001701. Glyco_hydro_9.
IPR018221. Glyco_hydro_9_AS.
[Graphical view]
PfamiPF00759. Glyco_hydro_9. 1 hit.
[Graphical view]
SUPFAMiSSF48208. SSF48208. 1 hit.
PROSITEiPS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q84R49-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFGRDPWGGP LEISNADSAT DDDRSRDLDR GALMRQLDET QQSWLLAGPG
60 70 80 90 100
DQAGKKKKKY VDLGCMVLDR KIFMWTVGTI LGVGLFIGFV MMIVKLVPHK
110 120 130 140 150
RPPPPPPDQY TQALHKALMF FNAQRSGPLP KHNGVSWRGN SCMKDGLSDS
160 170 180 190 200
TVRKSLVGGF YDAGDAIKFN YPMAWSMTML SWSVIEYKAK YEAIGELDHV
210 220 230 240 250
KELIKWGTDY LLKTFNSSAD TIDRIVAQVG VGDTSKGGAQ PNDHYCWMRP
260 270 280 290 300
EDIDYPRPVT ECHSCSDLAS EMAAALAAAS IVFKDSKTYS DKLVRGAKAL
310 320 330 340 350
YKFGRLQRGR YSPNGSDQAI FYNSTSYWDE FVWGGAWMYF ATGNNTYLSV
360 370 380 390 400
ATAPGMAKHA GAYWLDSPNY GVFTWDDKLP GAQVLLSRLR LFLSPGYPYE
410 420 430 440 450
EILRTFHNQT DNVMCSYLPM YNSFNFTKGG MIQLNHGRPQ PLQYVVNAAF
460 470 480 490 500
LASLYSDYLD AADTPGWYCG PTFYTTEVLR KFARSQLDYV LGKNPLKMSY
510 520 530 540 550
VVGFGNKYPK RAHHRGASIP HNGVKYGCKG GFKWRETKKP NPNILIGALV
560 570 580 590 600
AGPDRHDGFK DVRTNYNYTE PTLAANAGLV AALISLTNIH VKSGIDKNTI
610 620
FSAVPPMFPT PPPPPSAWKP
Length:620
Mass (Da):69,067
Last modified:June 1, 2003 - v1
Checksum:i2B5721EBD2ED7D52
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY387483 Genomic DNA. Translation: AAR07086.1.
AC118133 Genomic DNA. Translation: AAP03405.1.
DP000009 Genomic DNA. Translation: ABF98746.1.
AP008209 Genomic DNA. Translation: BAF13106.1.
CM000140 Genomic DNA. Translation: EEE59884.1.
AK070408 mRNA. Translation: BAG91933.1.
RefSeqiNP_001051192.1. NM_001057727.1.
UniGeneiOs.59414.

Genome annotation databases

EnsemblPlantsiOS03T0736300-01; OS03T0736300-01; OS03G0736300.
GeneIDi4334029.
KEGGiosa:4334029.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY387483 Genomic DNA. Translation: AAR07086.1.
AC118133 Genomic DNA. Translation: AAP03405.1.
DP000009 Genomic DNA. Translation: ABF98746.1.
AP008209 Genomic DNA. Translation: BAF13106.1.
CM000140 Genomic DNA. Translation: EEE59884.1.
AK070408 mRNA. Translation: BAG91933.1.
RefSeqiNP_001051192.1. NM_001057727.1.
UniGeneiOs.59414.

3D structure databases

ProteinModelPortaliQ84R49.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi39947.LOC_Os03g52630.1.

Protein family/group databases

CAZyiGH9. Glycoside Hydrolase Family 9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiOS03T0736300-01; OS03T0736300-01; OS03G0736300.
GeneIDi4334029.
KEGGiosa:4334029.

Organism-specific databases

GrameneiQ84R49.

Phylogenomic databases

eggNOGiNOG257354.
HOGENOMiHOG000021033.
InParanoidiQ84R49.
KOiK01179.
OMAiFIMMIVK.

Gene expression databases

ExpressionAtlasiQ84R49. baseline.

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR001701. Glyco_hydro_9.
IPR018221. Glyco_hydro_9_AS.
[Graphical view]
PfamiPF00759. Glyco_hydro_9. 1 hit.
[Graphical view]
SUPFAMiSSF48208. SSF48208. 1 hit.
PROSITEiPS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A complex history of rearrangement in an orthologous region of the maize, sorghum, and rice genomes."
    Ilic K., SanMiguel P.J., Bennetzen J.L.
    Proc. Natl. Acad. Sci. U.S.A. 100:12265-12270(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Sequence, annotation, and analysis of synteny between rice chromosome 3 and diverged grass species."
    The rice chromosome 3 sequencing consortium
    Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B., Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T., Fadrosh D., Bera J., Weaver B., Jin S.
    , Johri S., Reardon M., Webb K., Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T., Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R., Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J., Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S., Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M., Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M., Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L., O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R., Jin W., Lee H.R., Jiang J., Jackson S.
    Genome Res. 15:1284-1291(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  4. "The rice annotation project database (RAP-DB): 2008 update."
    The rice annotation project (RAP)
    Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: cv. Nipponbare.
  5. "The genomes of Oryza sativa: a history of duplications."
    Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.
    , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.
    PLoS Biol. 3:266-281(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  6. "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
    The rice full-length cDNA consortium
    Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Nipponbare.
  7. "OsGLU1, a putative membrane-bound endo-1,4-beta-D-glucanase from rice, affects plant internode elongation."
    Zhou H.-L., He S.-J., Cao Y.-R., Chen T., Du B.-X., Chu C.-C., Zhang J.-S., Chen S.-Y.
    Plant Mol. Biol. 60:137-151(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiGUN10_ORYSJ
AccessioniPrimary (citable) accession number: Q84R49
Secondary accession number(s): Q0DNT2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2006
Last sequence update: June 1, 2003
Last modified: January 7, 2015
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.