ID   GUN21_ORYSJ             Reviewed;         529 AA.
AC   Q84Q51; A0A0P0XFV9;
DT   05-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   24-JAN-2024, entry version 93.
DE   RecName: Full=Endoglucanase 21;
DE            EC=3.2.1.4;
DE   AltName: Full=Endo-1,4-beta glucanase 21;
DE   AltName: Full=OsGLU9;
DE   Flags: Precursor;
GN   Name=GLU9; OrderedLocusNames=Os08g0425300, LOC_Os08g32940;
GN   ORFNames=P0456B03.110;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [3]
RP   TISSUE SPECIFICITY.
RX   PubMed=16463105; DOI=10.1007/s11103-005-2972-x;
RA   Zhou H.-L., He S.-J., Cao Y.-R., Chen T., Du B.-X., Chu C.-C., Zhang J.-S.,
RA   Chen S.-Y.;
RT   "OsGLU1, a putative membrane-bound endo-1,4-beta-D-glucanase from rice,
RT   affects plant internode elongation.";
RL   Plant Mol. Biol. 60:137-151(2006).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC         cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- TISSUE SPECIFICITY: Expressed in roots and flowers.
CC       {ECO:0000269|PubMed:16463105}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 9 (cellulase E) family.
CC       {ECO:0000255|PROSITE-ProRule:PRU10140, ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AP004463; BAC55745.1; -; Genomic_DNA.
DR   EMBL; AP014964; BAT05499.1; -; Genomic_DNA.
DR   RefSeq; XP_015649456.1; XM_015793970.1.
DR   AlphaFoldDB; Q84Q51; -.
DR   SMR; Q84Q51; -.
DR   STRING; 39947.Q84Q51; -.
DR   CAZy; GH9; Glycoside Hydrolase Family 9.
DR   GlyCosmos; Q84Q51; 1 site, No reported glycans.
DR   PaxDb; 39947-Q84Q51; -.
DR   EnsemblPlants; Os08t0425300-00; Os08t0425300-00; Os08g0425300.
DR   GeneID; 107276216; -.
DR   Gramene; Os08t0425300-00; Os08t0425300-00; Os08g0425300.
DR   KEGG; osa:107276216; -.
DR   eggNOG; ENOG502QRF6; Eukaryota.
DR   HOGENOM; CLU_008926_1_4_1; -.
DR   InParanoid; Q84Q51; -.
DR   OMA; YLVKAHT; -.
DR   OrthoDB; 1347382at2759; -.
DR   Proteomes; UP000000763; Chromosome 8.
DR   Proteomes; UP000059680; Chromosome 8.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR   GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR   GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR   Gene3D; 1.50.10.10; -; 1.
DR   InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR   InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR   InterPro; IPR001701; Glyco_hydro_9.
DR   InterPro; IPR018221; Glyco_hydro_9_His_AS.
DR   PANTHER; PTHR22298; ENDO-1,4-BETA-GLUCANASE; 1.
DR   PANTHER; PTHR22298:SF107; ENDOGLUCANASE 21; 1.
DR   Pfam; PF00759; Glyco_hydro_9; 1.
DR   SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
DR   PROSITE; PS60032; GH9_1; 1.
DR   PROSITE; PS00592; GH9_2; 1.
DR   Genevisible; Q84Q51; OS.
PE   2: Evidence at transcript level;
KW   Carbohydrate metabolism; Cell wall biogenesis/degradation;
KW   Cellulose degradation; Glycoprotein; Glycosidase; Hydrolase;
KW   Polysaccharide degradation; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..24
FT                   /evidence="ECO:0000255"
FT   CHAIN           25..529
FT                   /note="Endoglucanase 21"
FT                   /id="PRO_0000249298"
FT   ACT_SITE        89
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10140"
FT   ACT_SITE        429
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10059"
FT   ACT_SITE        481
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10059"
FT   ACT_SITE        490
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10059"
FT   CARBOHYD        342
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   529 AA;  57924 MW;  0C4AD0D5719D5A28 CRC64;
     MVAAMTMCAA VAVLLVLTST MAAAAGDGDG DGGGFDYKKA LHSGLLYFEA QRSGHLPYNQ
     RVRWRGHSGL ADGLQQGVDL VGGYYDAGDN VKFGLPMAFT MTMLSWAAAE FWDEIAAAGE
     RRHVLEAIKW GTDYLVKAHT AADELWAEVG DGDTDHYCWQ RPEDMTTSRQ AYKVDRDNPG
     SDVAGETAAA LAAASIVFRR SKPRYSRLLL RHAEQLFDFG DRYRGKYDSS IGEVRAYYAS
     VSGYGDELLW AALWLHRATG RRGYLDYAVA MADELGGVGW AVTEFSWDVK YAGLQILAAK
     VLMDGGDHPA AHAATLEQYR SKAEHYLCAC LGKNAAAGDN VNRTAGGMLF VRRWNNMQYV
     TNAAFLLTVY SRYLRDSGGD TIRCSGGAMA TGDELAAMAR AQADYVLGDN PAGVSYMVGY
     GRRFPRRVHH RGASMVSHRA DGRFVGCVQG YDRWFRRGGA NPNVVAGAIV GGPDDRDRFR
     DSRDNYMQTE ACTYNTAPMV GVFAHLHAQK MAARTANNNA DRSMIKRVD
//