Q84P25 (4CLL2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-coumarate--CoA ligase-like 2
EC=6.2.1.-

Gene names

Name:	4CLL2
Ordered Locus Names:	At1g20480
ORF Names:	F5M15.29

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	565 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Subcellular location	Peroxisome Potential.
Domain	Both substrate-binding domains (SBD1 and SBD2) are involved in the substrate recognition, and are sufficient to confer the substrate specificity By similarity.
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family.
Sequence caution	The sequence AAF79612.1 differs from that shown. Reason: Erroneous gene model prediction. The predicted gene has been split into 3 genes: At1g20480, At1g20490 and At1g20500.

Ontologies

Keywords
Cellular component	Peroxisome
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	peroxisome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ligase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 565	565	4-coumarate--CoA ligase-like 2		PRO_0000299175
Regions
	Nucleotide binding	221 – 229	9	ATP By similarity
	Nucleotide binding	360 – 365	6	ATP By similarity
	Region	288 – 359	72	SBD1 By similarity
	Region	360 – 424	65	SBD2 By similarity
	Motif	563 – 565	3	Microbody targeting signal Potential
Sites
	Binding site	445	1	ATP By similarity
	Binding site	460	1	ATP By similarity
	Binding site	551	1	ATP By similarity
Experimental info
	Sequence conflict	13	1	E → V in AAP03016. Ref.1
	Sequence conflict	74	1	R → Q in AAP03016. Ref.1
	Sequence conflict	182	1	S → T in AAP03016. Ref.1
	Sequence conflict	355	1	V → F in AAP03016. Ref.1
	Sequence conflict	505	1	M → V in AAP03016. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q84P25 [UniParc]. Last modified September 11, 2007. Version 2. Checksum: 0226F2C09AA3031B	FASTA	565	61,438
	10 20 30 40 50 60 MAVKHGVDGD GSEIESRTLA VDRKSGFCES TSIFYSKREP MALPPNQFLD VTSFIASQPH 70 80 90 100 110 120 RGKTVFVDAV TGRRLSFPEL WLGVERVAGC LYALGVRKGN VVIILSPNSI LFPIVSLSVM 130 140 150 160 170 180 SLGAIITTAN PINTSDEISK QIGDSRPVLA FTTCKLVSKL AAASNFNLPV VLMDDYHVPS 190 200 210 220 230 240 QSYGDRVKLV GRLETMIETE PSESRVKQRV NQDDTAALLY SSGTTGTSKG VMLSHRNLIA 250 260 270 280 290 300 LVQAYRARFG LEQRTICTIP MCHIFGFGGF ATGLIALGWT IVVLPKFDMA KLLSAVETHR 310 320 330 340 350 360 SSYLSLVPPI VVAMVNGANE INSKYDLSSL HTVVAGGAPL SREVTEKFVE NYPKVKILQG 370 380 390 400 410 420 YGLTESTAIA ASMFNKEETK RYGASGLLAP NVEGKIVDPD TGRVLGVNQT GELWIRSPTV 430 440 450 460 470 480 MKGYFKNKEA TASTIDSEGW LKTGDLCYID GDGFVFVVDR LKELIKCNGY QVAPAELEAL 490 500 510 520 530 540 LLAHPEIADA AVIPIPDMKA GQYPMAYIVR KVGSNLSESE IMGFVAKQVS PYKKIRKVTF 550 560 LASIPKNPSG KILRRELTKL TTSKL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Arabidopsis contains a large superfamily of acyl-activating enzymes. Phylogenetic and biochemical analysis reveals a new class of acyl-coenzyme a synthetases." Shockey J.M., Fulda M.S., Browse J. Plant Physiol. 132:1065-1076(2003) [PubMed: 12805634] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY ORGANIZATION. Strain: cv. Wassilewskija.
[2]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"The substrate specificity-determining amino acid code of 4-coumarate:CoA ligase." Schneider K., Hoevel K., Witzel K., Hamberger B., Schomburg D., Kombrink E., Stuible H.-P. Proc. Natl. Acad. Sci. U.S.A. 100:8601-8606(2003) [PubMed: 12819348] [Abstract] Cited for: GENE FAMILY ORGANIZATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY250833 mRNA. Translation: AAP03016.1. AC027665 Genomic DNA. Translation: AAF79612.1. Sequence problems. CP002684 Genomic DNA. Translation: AEE29977.1.
IPI	IPI00518444.
PIR	D86338.
RefSeq	NP_173472.1. NM_101898.3.
UniGene	At.41707. At.69772.
3D structure databases
HSSP	HSSP built from PDB template 1LCI based on UniProtKB P08659.
ProteinModelPortal	Q84P25.
SMR	Q84P25. Positions 33-559.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q84P25. 1 interaction.
STRING	Q84P25.
Proteomic databases
PRIDE	Q84P25.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G20480.1; AT1G20480.1; AT1G20480.
GeneID	838636.
GenomeReviews	Gene locus AT1G20480 in contig CT485782_GR.
KEGG	ath:AT1G20480.
NMPDR	fig|3702.1.peg.2394.
Organism-specific databases
TAIR	At1g20480.
Phylogenomic databases
eggNOG	KOG1176.
GeneTree	EPGT00070000028073.
HOGENOM	HBG547964.
InParanoid	Q84P25.
OMA	SAVETHR.
PhylomeDB	Q84P25.
ProtClustDB	CLSN2679410.
Gene expression databases
ArrayExpress	Q84P25.
Genevestigator	Q84P25.
Family and domain databases
InterPro	IPR020845. AMP-binding_CS. IPR000873. AMP-dep_Synth/Lig. [Graphical view]
Pfam	PF00501. AMP-binding. 1 hit. [Graphical view]
PROSITE	PS00455. AMP_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

4CLL2_ARATH

Accession

Primary (citable) accession number: Q84P25
Secondary accession number(s): Q9LMV7

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	September 11, 2007
Last modified:	December 14, 2011
This is version 50 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program