Reviewed, UniProtKB/Swiss-Prot Q84NP7 (AMPD_ORYSJ)

Names and origin

Protein names

Recommended name:
    Probable AMP deaminase
    EC=3.5.4.6

Gene names

Name:	AMPD
Ordered Locus Names:	Os07g0693500, LOC_Os07g49270
ORF Names:	P0034A04.129

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	815 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	AMP deaminase plays a critical role in energy metabolism By similarity.
Catalytic activity	AMP + H2O = IMP + NH3.
Pathway	Purine metabolism; IMP biosynthesis via salvage pathway; IMP from AMP: step 1/1.
Subunit structure	Homodimer By similarity.
Subcellular location	Membrane; Single-pass membrane protein. Note: Might be associated with the inner mitochondrial membrane By similarity.
Sequence similarities	Belongs to the adenosine and AMP deaminases family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Cellular component	Membrane
Domain	Transmembrane
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Gene Ontology (GO)
Biological process	purine ribonucleoside monophosphate biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	AMP deaminase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 815	815	Probable AMP deaminase		PRO_0000238456
Regions
	Transmembrane	5 – 27	23	Potential
	Compositional bias	55 – 69	15	Asp-rich
	Compositional bias	125 – 130	6	Poly-Asp
	Compositional bias	604 – 616	13	Tyr-rich
Sites
	Metal binding	367	1	Zinc; catalytic By similarity
	Metal binding	369	1	Zinc; catalytic By similarity
	Metal binding	635	1	Zinc; catalytic By similarity
	Metal binding	712	1	Zinc; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q84NP7-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: E9C9C55382961A7F	FASTA	815	93,854
	10 20 30 40 50 60 MDSTYALHLA VATLLGASFA AASAYYMHRK TLDQLLRFAR SLDRDHRRRN RHLLDADDDD 70 80 90 100 110 120 DDDPPRDHDR RTTLPIPPGL PPLHTGREGK PIISPASTKR VGPLVRPTTP RSPVPTVSAF 130 140 150 160 170 180 ETIEDSDDDD ENIAPDAKNN AVSLLTNGTI GSDPLPGKAS QNGDTKPVPS TNMIRSQSAT 190 200 210 220 230 240 GSLHGAQHNP VAADILRKEP EHETFSRINI TAVETPSPDE IEAYKVLQKC LELREKYMFR 250 260 270 280 290 300 EEVAPWEKEI ITDPSTPKPN PNPFYYEQQT KTEHHFEMVD GVIHVYPNKD AKERIYPVAD 310 320 330 340 350 360 ATTFFTDMHY ILRVLAAGDI RTVCYKRLNL LEQKFNLHLM VNADRELLAQ KAAPHRDFYN 370 380 390 400 410 420 VRKVDTHVHH SACMNQKHLL RFIKSKLRKE PDEVVIFRDG TYLTLKEVFE SLDLTGYDLN 430 440 450 460 470 480 VDLLDVHADK STFHRFDKFN LKYNPCGQSR LREIFLKQDN LIQGRFLAEL TKEVFSDLEA 490 500 510 520 530 540 SKYQMAEYRI SIYGRKKSEW DQMASWIVNN ELYSENVVWL IQIPRIYNVY REMGTINSFQ 550 560 570 580 590 600 NLLDNIFLPL FEVTVDPASH PQLHVFLQQV VGLDLVDDES KPERRPTKHM PTPEQWTNVF 610 620 630 640 650 660 NPAYAYYVYY CYANLYTLNK LRESKGMTTI KLRPHCGEAG DIDHLAAAFL TSHNIAHGVN 670 680 690 700 710 720 LKKSPVLQYL YYLAQIGLAM SPLSNNSLFI DYHRNPFPTF FLRGLNVSLS TDDPLQIHLT 730 740 750 760 770 780 KEPLVEEYSI AASLWKLSSC DLCEIARNSV YQSGFSHRLK SHWIGRNYYK RGHDGNDIHQ 790 800 810 TNVPHIRIEF RHTIWKEEME LIHLRNVDIP EEIDR

References

[1]	"Oryza sativa nipponbare chromosome 7 genomic DNA sequence." The international rice genome sequencing project (IRGSP) consortium Submitted (NOV-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed: 12869764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP004333 Genomic DNA. Translation: BAC75568.1. AK064333 mRNA. No translation available. AK102007 mRNA. No translation available.
RefSeq	NP_001060730.1.
UniGene	Os.8779
3D structure databases
SMR	Q84NP7. Positions 187-813.
ModBase	Search...
Genome annotation databases
GeneID	4344386.
KEGG	osa:4344386.
NMPDR	fig|39947.1.peg.2388.
Organism-specific databases
Gramene	Q84NP7.
Family and domain databases
InterPro	IPR006650. A/AMP_deam_AS. IPR001365. A/AMP_deaminase. IPR006329. AMP_deaminase. [Graphical view]
Pfam	PF00962. A_deaminase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01429. AMP_deaminase. 1 hit.
PROSITE	PS00485. A_DEAMINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPD_ORYSJ

Accession

Primary (citable) accession number: Q84NP7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	June 1, 2003
Last modified:	October 13, 2009
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)