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Protein

Dynamin-like protein ARC5

Gene

ARC5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable GTPase component of both plastid and peroxisme division machinery. Required for the last steps of plastid division specifically in mesophyll-cell, when the narrow isthmus breaks, facilitating the separation of the daughter plastids. Necessary for peroxisome activities. Seems to influence stromule (stroma-filled tubular extensions of the plastid envelope membrane) length and frequency.6 Publications

Catalytic activityi

GTP + H2O = GDP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi55 – 628GTPSequence analysis
Nucleotide bindingi160 – 1645GTPSequence analysis
Nucleotide bindingi231 – 2344GTPSequence analysis

GO - Molecular functioni

  • GTP binding Source: UniProtKB-KW
  • hydrolase activity Source: UniProtKB-KW
  • identical protein binding Source: UniProtKB

GO - Biological processi

  • chloroplast fission Source: TAIR
  • circadian rhythm Source: TAIR
  • peroxisome fission Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Motor protein

Keywords - Biological processi

Peroxisome biogenesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT3G19720-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Dynamin-like protein ARC5 (EC:3.6.5.5)
Alternative name(s):
Dynamin-related protein 5B
Protein ACCUMULATION AND REPLICATION OF CHLOROPLASTS 5
Gene namesi
Name:ARC5
Synonyms:DRP5B
Ordered Locus Names:At3g19720
ORF Names:MMB12.21
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G19720.

Subcellular locationi

GO - Cellular componenti

  • chloroplast Source: TAIR
  • chloroplast outer membrane Source: TAIR
  • peroxisome Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Cytoplasm, Membrane, Peroxisome, Plastid, Plastid outer membrane

Pathology & Biotechi

Disruption phenotypei

Defects in plastid constriction leading to large and dumbbell-shaped chloroplasts in mesophyll-cell only. Reduced number of large chloroplasts in green tissues. Mostly normal vascular and epidermal chloroplasts and normal plastid size in non green tissues, sometimes exhibiting alteration in stromule length and frequency (e.g. increase in the frequency of stromules in cells of stamen filaments). Presence of highly clustered peroxisomes unable to complete fission and/or enlarged peroxisomes. Impaired peroxisome-related functions, such as photorespiration and fatty acid beta-oxidation.6 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 777777Dynamin-like protein ARC5PRO_0000407220Add
BLAST

Proteomic databases

PaxDbiQ84N64.
PRIDEiQ84N64.

PTM databases

iPTMnetiQ84N64.

Expressioni

Gene expression databases

ExpressionAtlasiQ84N64. baseline and differential.
GenevisibleiQ84N64. AT.

Interactioni

Subunit structurei

Forms a homodimer and heterodimers with DRP3A and DRP3B on peroxisomes. Interacts also with FIS1A (but not FIS1B) and PEX11 proteins (PEX11A, PEX11B, PEX11C, PEX11D and PEX11E) on peroxisomes.1 Publication

GO - Molecular functioni

  • identical protein binding Source: UniProtKB

Protein-protein interaction databases

BioGridi6842. 12 interactions.
STRINGi3702.AT3G19720.1.

Structurei

3D structure databases

ProteinModelPortaliQ84N64.
SMRiQ84N64. Positions 33-359.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini45 – 343299Dynamin-type GAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili300 – 32021Sequence analysisAdd
BLAST
Coiled coili728 – 76538Sequence analysisAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0446. Eukaryota.
COG0699. LUCA.
HOGENOMiHOG000029906.
InParanoidiQ84N64.
PhylomeDBiQ84N64.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR001401. Dynamin_GTPase.
IPR022812. Dynamin_SF.
IPR030381. G_DYNAMIN_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR11566. PTHR11566. 3 hits.
PfamiPF00350. Dynamin_N. 1 hit.
[Graphical view]
PRINTSiPR00195. DYNAMIN.
SMARTiSM00053. DYNc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS51718. G_DYNAMIN_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q84N64-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAEVSAKSVT VEEMAEEDDA AIEERWSLYE AYNELHALAQ ELETPFEAPA
60 70 80 90 100
VLVVGQQTDG KSALVEALMG FQFNHVGGGT KTRRPITLHM KYDPQCQFPL
110 120 130 140 150
CHLGSDDDPS VSLPKSLSQI QAYIEAENMR LEQEPCSPFS AKEIIVKVQY
160 170 180 190 200
KYCPNLTIID TPGLIAPAPG LKNRALQVQA RAVEALVRAK MQHKEFIILC
210 220 230 240 250
LEDSSDWSIA TTRRIVMQVD PELSRTIVVS TKLDTKIPQF SCSSDVEVFL
260 270 280 290 300
SPPASALDSS LLGDSPFFTS VPSGRVGYGQ DSVYKSNDEF KQAVSLREME
310 320 330 340 350
DIASLEKKLG RLLTKQEKSR IGISKLRLFL EELLWKRYKE SVPLIIPLLG
360 370 380 390 400
KEYRSTVRKL DTVSKELSSL DEAKLKERGR TFHDLFLTKL SLLLKGTVVA
410 420 430 440 450
PPDKFGETLQ DERTQGGAFV GTDGLQFSHK LIPNAGMRLY GGAQYHRAMA
460 470 480 490 500
EFRFLVGAIK CPPITREEIV NACGVEDIHD GTNYSRTACV IAVAKARETF
510 520 530 540 550
EPFLHQLGAR LLHILKRLLP ISVYLLQKEG EYLSGHEVFL KRVASAFNSF
560 570 580 590 600
VESTEKSCRD KCMEDLASTT RYVTWSLHNK NRAGLRQFLD SFGGTEHNTT
610 620 630 640 650
SGNAIGFSLP QDALGGTTDT KSRSDVKLSH LASNIDSGSS IQTTEMRLAD
660 670 680 690 700
LLDSTLWNRK LAPSSERIVY ALVQQIFQGI REYFLASAEL KFNCFLLMPI
710 720 730 740 750
VDKLPALLRE ELENAFEDDL DSIFDITNLR QSLDQKKRST EIELRRIKRI
760 770
KEKFRVMNEK LNSHEFAQNL KAPSVQH
Length:777
Mass (Da):87,171
Last modified:April 5, 2011 - v2
Checksum:i8C5CBF0852EA7B8A
GO
Isoform 2 (identifier: Q84N64-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     601-636: Missing.

Note: Derived from EST data. No experimental confirmation available.
Show »
Length:741
Mass (Da):83,515
Checksum:i4717452254DBAB07
GO

Sequence cautioni

The sequence BAB02559.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti121 – 1211Q → H in AAO89221 (PubMed:12642673).Curated
Sequence conflicti433 – 4331P → Q in AAO89221 (PubMed:12642673).Curated
Sequence conflicti747 – 7471I → V in AAO89221 (PubMed:12642673).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei601 – 63636Missing in isoform 2. CuratedVSP_040936Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY212885 mRNA. Translation: AAO89221.1.
AP000417 Genomic DNA. Translation: BAB02559.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE76279.1.
CP002686 Genomic DNA. Translation: AEE76280.1.
PIRiT52402.
RefSeqiNP_188606.2. NM_112862.3. [Q84N64-1]
NP_850615.2. NM_180284.2. [Q84N64-2]
UniGeneiAt.38361.

Genome annotation databases

EnsemblPlantsiAT3G19720.1; AT3G19720.1; AT3G19720. [Q84N64-1]
GeneIDi821509.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY212885 mRNA. Translation: AAO89221.1.
AP000417 Genomic DNA. Translation: BAB02559.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE76279.1.
CP002686 Genomic DNA. Translation: AEE76280.1.
PIRiT52402.
RefSeqiNP_188606.2. NM_112862.3. [Q84N64-1]
NP_850615.2. NM_180284.2. [Q84N64-2]
UniGeneiAt.38361.

3D structure databases

ProteinModelPortaliQ84N64.
SMRiQ84N64. Positions 33-359.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi6842. 12 interactions.
STRINGi3702.AT3G19720.1.

PTM databases

iPTMnetiQ84N64.

Proteomic databases

PaxDbiQ84N64.
PRIDEiQ84N64.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G19720.1; AT3G19720.1; AT3G19720. [Q84N64-1]
GeneIDi821509.

Organism-specific databases

TAIRiAT3G19720.

Phylogenomic databases

eggNOGiKOG0446. Eukaryota.
COG0699. LUCA.
HOGENOMiHOG000029906.
InParanoidiQ84N64.
PhylomeDBiQ84N64.

Enzyme and pathway databases

BioCyciARA:AT3G19720-MONOMER.

Miscellaneous databases

PROiQ84N64.

Gene expression databases

ExpressionAtlasiQ84N64. baseline and differential.
GenevisibleiQ84N64. AT.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR001401. Dynamin_GTPase.
IPR022812. Dynamin_SF.
IPR030381. G_DYNAMIN_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR11566. PTHR11566. 3 hits.
PfamiPF00350. Dynamin_N. 1 hit.
[Graphical view]
PRINTSiPR00195. DYNAMIN.
SMARTiSM00053. DYNc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS51718. G_DYNAMIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "ARC5, a cytosolic dynamin-like protein from plants, is part of the chloroplast division machinery."
    Gao H., Kadirjan-Kalbach D., Froehlich J.E., Osteryoung K.W.
    Proc. Natl. Acad. Sci. U.S.A. 100:4328-4333(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION.
    Strain: cv. Columbia and cv. Landsberg erecta.
  2. "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones."
    Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.
    DNA Res. 7:217-221(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "A genetic analysis of chloroplast division and expansion in Arabidopsis thaliana."
    Pyke K.A., Leech R.M.
    Plant Physiol. 104:201-207(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
    Strain: cv. Landsberg erecta.
  5. "Characterization of chloroplast division using the Arabidopsis mutant arc5."
    Robertson E.J., Rutherford S.M., Leech R.M.
    Plant Physiol. 112:149-159(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
    Strain: cv. Landsberg erecta.
  6. "The distinctive roles of five different ARC genes in the chloroplast division process in Arabidopsis."
    Marrison J.L., Rutherford S.M., Robertson E.J., Lister C., Dean C., Leech R.M.
    Plant J. 18:651-662(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  7. "PDV1 and PDV2 mediate recruitment of the dynamin-related protein ARC5 to the plastid division site."
    Miyagishima S.-Y., Froehlich J.E., Osteryoung K.W.
    Plant Cell 18:2517-2530(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  8. "Effects of arc3, arc5 and arc6 mutations on plastid morphology and stromule formation in green and nongreen tissues of Arabidopsis thaliana."
    Holzinger A., Kwok E.Y., Hanson M.R.
    Photochem. Photobiol. 84:1324-1335(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
    Strain: cv. Columbia and cv. Landsberg erecta.
  9. "Arabidopsis ARC6 coordinates the division machineries of the inner and outer chloroplast membranes through interaction with PDV2 in the intermembrane space."
    Glynn J.M., Froehlich J.E., Osteryoung K.W.
    Plant Cell 20:2460-2470(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
    Strain: cv. Columbia.
  10. "Peroxisome division and proliferation in plants."
    Aung K., Zhang X., Hu J.
    Biochem. Soc. Trans. 38:817-822(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.
  11. "The Arabidopsis chloroplast division protein DYNAMIN-RELATED PROTEIN5B also mediates peroxisome division."
    Zhang X., Hu J.
    Plant Cell 22:431-442(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, SELF-INTERACTION, INTERACTION WITH FIS1A; DRP3A; DRP3B; PEX11A; PEX11B; PEX11C; PEX11D AND PEX11E, SUBCELLULAR LOCATION.
    Strain: cv. Columbia and cv. Landsberg erecta.

Entry informationi

Entry nameiARC5_ARATH
AccessioniPrimary (citable) accession number: Q84N64
Secondary accession number(s): Q3EB39, Q9LJM3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 5, 2011
Last sequence update: April 5, 2011
Last modified: May 11, 2016
This is version 88 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.