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Q84N49

- CAF1P_MAIZE

UniProt

Q84N49 - CAF1P_MAIZE

Protein

CRS2-associated factor 1, chloroplastic

Gene

CAF1

Organism
Zea mays (Maize)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 49 (01 Oct 2014)
      Sequence version 1 (01 Jun 2003)
      Previous versions | rss
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    Functioni

    Required for the splicing of group IIB introns in chloroplasts. Forms splicing particles with CRS2. Interacts with RNA and confers intron specificity of the splicing particles.1 Publication

    GO - Molecular functioni

    1. RNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. Group II intron splicing Source: EnsemblPlants/Gramene
    2. mRNA processing Source: UniProtKB-KW

    Keywords - Molecular functioni

    Ribonucleoprotein

    Keywords - Biological processi

    mRNA processing, mRNA splicing

    Keywords - Ligandi

    RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    CRS2-associated factor 1, chloroplastic
    Alternative name(s):
    Chloroplastic group IIA intron splicing facilitator CRS2-associated factor 1
    Gene namesi
    Name:CAF1
    OrganismiZea mays (Maize)
    Taxonomic identifieri4577 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogoneaeZea

    Organism-specific databases

    GrameneiQ84N49.
    MaizeGDBi886554.

    Subcellular locationi

    Plastidchloroplast stroma 1 Publication

    GO - Cellular componenti

    1. chloroplast stroma Source: UniProtKB-SubCell
    2. ribonucleoprotein complex Source: UniProtKB-KW

    Keywords - Cellular componenti

    Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 5454ChloroplastSequence AnalysisAdd
    BLAST
    Chaini55 – 674620CRS2-associated factor 1, chloroplasticPRO_0000283615Add
    BLAST

    Interactioni

    Subunit structurei

    Interacts with CRS2 and RNA. Part of large ribonucleo-protein complexes that include group IIB introns, CRS2 and CAF1.2 Publications

    Protein-protein interaction databases

    DIPiDIP-48740N.

    Structurei

    3D structure databases

    ProteinModelPortaliQ84N49.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini183 – 27997CRM 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini301 – 39797CRM 2PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni554 – 57623CRS2 bindingBy similarityAdd
    BLAST

    Sequence similaritiesi

    Contains 2 CRM domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Transit peptide

    Phylogenomic databases

    HOGENOMiHOG000195646.

    Family and domain databases

    Gene3Di3.30.110.60. 2 hits.
    InterProiIPR001890. RNA-binding_CRM.
    [Graphical view]
    PfamiPF01985. CRS1_YhbY. 2 hits.
    [Graphical view]
    ProDomiPD010559. RNA-binding_CRM. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SMARTiSM01103. CRS1_YhbY. 2 hits.
    [Graphical view]
    SUPFAMiSSF75471. SSF75471. 2 hits.
    PROSITEiPS51295. CRM. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q84N49-1 [UniParc]FASTAAdd to Basket

    « Hide

    MATARLPSRS FLSPAQQSYP RLPASVRLCL SHHEQPPTGP KRHRRAATSH    50
    PAFSAAARGR AKKIPIADTD EPAAGVRVTD RGISYRLDGA PFEFQYSYTE 100
    APRARPVALR EAPFMPFGPE ATPRPWTGRK PLPKSRKELP EFDSFVLPAP 150
    GKKGVKPVQS PGPFLAGMEP RYQSVSREDI LGEPLTKEEV SELVKGSLKS 200
    KRQLNMGRDG LTHNMLENIH SHWKRKRVCK IKCKGVCTID MDNICHQLEE 250
    KVGGKVIHRQ GGVIFLFRGR NYNYRTRPCF PLMLWKPVAP VYPRLVTKVP 300
    GGLTPDEATE MRTRGHQLPP ICKLGKNGVY ANLVNQVREA FEACDLVRVD 350
    CSGLNKSDCR KIGAKLKDLV PCILLSFEFE HILMWRGSDW KSSLPPLENS 400
    YEVTKVQESF SGKESNEKVT HSGNVLAQIE LVSAATSHKN WNLDEGQEKF 450
    KDSTDSDMVL NSAKDVPALF HSTGISRTEP SADIPLEYSP LNPVCDIMDP 500
    SLNCRSIPTN NCESRALVEK SEHCPDDYNL EMKRKRNDGT KGTVVLNSGS 550
    KVEGLLCLLE QAIHSGRALV LSEDELADSD LVYEKSVAFT KSIPRRLVFE 600
    NTRRKSSARR NVPDNHARTK THLVENKMLS SPVENKFIVN GGSAMQTIDH 650
    GQEFLSDVVH QGTLRVDELA KLLA 674
    Length:674
    Mass (Da):75,145
    Last modified:June 1, 2003 - v1
    Checksum:i0C3454B196954E56
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY264368 mRNA. Translation: AAP22135.1.
    RefSeqiNP_001105220.1. NM_001111750.1.
    UniGeneiZm.96493.

    Genome annotation databases

    GeneIDi542118.
    KEGGizma:542118.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY264368 mRNA. Translation: AAP22135.1 .
    RefSeqi NP_001105220.1. NM_001111750.1.
    UniGenei Zm.96493.

    3D structure databases

    ProteinModelPortali Q84N49.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-48740N.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 542118.
    KEGGi zma:542118.

    Organism-specific databases

    Gramenei Q84N49.
    MaizeGDBi 886554.

    Phylogenomic databases

    HOGENOMi HOG000195646.

    Family and domain databases

    Gene3Di 3.30.110.60. 2 hits.
    InterProi IPR001890. RNA-binding_CRM.
    [Graphical view ]
    Pfami PF01985. CRS1_YhbY. 2 hits.
    [Graphical view ]
    ProDomi PD010559. RNA-binding_CRM. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SMARTi SM01103. CRS1_YhbY. 2 hits.
    [Graphical view ]
    SUPFAMi SSF75471. SSF75471. 2 hits.
    PROSITEi PS51295. CRM. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Group II intron splicing factors derived by diversification of an ancient RNA-binding domain."
      Ostheimer G.J., Williams-Carrier R., Belcher S., Osborne E., Gierke J., Barkan A.
      EMBO J. 22:3919-3929(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH CRS2.
      Strain: cv. B73.
    2. "Formation of the CRS2-CAF2 group II intron splicing complex is mediated by a 22-amino acid motif in the COOH-terminal region of CAF2."
      Ostheimer G.J., Rojas M., Hadjivassiliou H., Barkan A.
      J. Biol. Chem. 281:4732-4738(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CRS2.

    Entry informationi

    Entry nameiCAF1P_MAIZE
    AccessioniPrimary (citable) accession number: Q84N49
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 3, 2007
    Last sequence update: June 1, 2003
    Last modified: October 1, 2014
    This is version 49 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3