Q84N49 (CAF1P_MAIZE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
CRS2-associated factor 1, chloroplastic

Alternative name(s):
Chloroplastic group IIA intron splicing facilitator CRS2-associated factor 1

Gene names

Name:

CAF1

Organism

Zea mays (Maize)

Taxonomic identifier

4577 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › PACMAD clade › Panicoideae › Andropogoneae › Zea

Protein attributes

Sequence length	674 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for the splicing of group IIB introns in chloroplasts. Forms splicing particles with CRS2. Interacts with RNA and confers intron specificity of the splicing particles. Ref.1
Subunit structure	Interacts with CRS2 and RNA. Part of large ribonucleo-protein complexes that include group IIB introns, CRS2 and CAF1. Ref.1 Ref.2
Subcellular location	Plastid › chloroplast stroma Ref.1.
Sequence similarities	Contains 2 CRM domains.

Ontologies

Keywords
Biological process	mRNA processing mRNA splicing
Cellular component	Chloroplast Plastid
Domain	Repeat Transit peptide
Ligand	RNA-binding
Molecular function	Ribonucleoprotein
Gene Ontology (GO)
Biological_process	RNA splicing Inferred from electronic annotation. Source: UniProtKB-KW mRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	chloroplast stroma Inferred from electronic annotation. Source: UniProtKB-SubCell ribonucleoprotein complex Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 54	54	Chloroplast Potential
	Chain	55 – 674	620	CRS2-associated factor 1, chloroplastic		PRO_0000283615
Regions
	Domain	183 – 279	97	CRM 1
	Domain	301 – 397	97	CRM 2
	Region	554 – 576	23	CRS2 binding By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q84N49 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 0C3454B196954E56	FASTA	674	75,145
	10 20 30 40 50 60 MATARLPSRS FLSPAQQSYP RLPASVRLCL SHHEQPPTGP KRHRRAATSH PAFSAAARGR 70 80 90 100 110 120 AKKIPIADTD EPAAGVRVTD RGISYRLDGA PFEFQYSYTE APRARPVALR EAPFMPFGPE 130 140 150 160 170 180 ATPRPWTGRK PLPKSRKELP EFDSFVLPAP GKKGVKPVQS PGPFLAGMEP RYQSVSREDI 190 200 210 220 230 240 LGEPLTKEEV SELVKGSLKS KRQLNMGRDG LTHNMLENIH SHWKRKRVCK IKCKGVCTID 250 260 270 280 290 300 MDNICHQLEE KVGGKVIHRQ GGVIFLFRGR NYNYRTRPCF PLMLWKPVAP VYPRLVTKVP 310 320 330 340 350 360 GGLTPDEATE MRTRGHQLPP ICKLGKNGVY ANLVNQVREA FEACDLVRVD CSGLNKSDCR 370 380 390 400 410 420 KIGAKLKDLV PCILLSFEFE HILMWRGSDW KSSLPPLENS YEVTKVQESF SGKESNEKVT 430 440 450 460 470 480 HSGNVLAQIE LVSAATSHKN WNLDEGQEKF KDSTDSDMVL NSAKDVPALF HSTGISRTEP 490 500 510 520 530 540 SADIPLEYSP LNPVCDIMDP SLNCRSIPTN NCESRALVEK SEHCPDDYNL EMKRKRNDGT 550 560 570 580 590 600 KGTVVLNSGS KVEGLLCLLE QAIHSGRALV LSEDELADSD LVYEKSVAFT KSIPRRLVFE 610 620 630 640 650 660 NTRRKSSARR NVPDNHARTK THLVENKMLS SPVENKFIVN GGSAMQTIDH GQEFLSDVVH 670 QGTLRVDELA KLLA

References

[1]	"Group II intron splicing factors derived by diversification of an ancient RNA-binding domain." Ostheimer G.J., Williams-Carrier R., Belcher S., Osborne E., Gierke J., Barkan A. EMBO J. 22:3919-3929(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH CRS2. Strain: cv. B73.
[2]	"Formation of the CRS2-CAF2 group II intron splicing complex is mediated by a 22-amino acid motif in the COOH-terminal region of CAF2." Ostheimer G.J., Rojas M., Hadjivassiliou H., Barkan A. J. Biol. Chem. 281:4732-4738(2006) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH CRS2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY264368 mRNA. Translation: AAP22135.1.
RefSeq	NP_001105220.1. NM_001111750.1.
UniGene	Zm.96493.
3D structure databases
ProteinModelPortal	Q84N49.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-48740N.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	542118.
KEGG	zma:542118.
Organism-specific databases
Gramene	Q84N49.
MaizeGDB	886554.
Phylogenomic databases
HOGENOM	HOG000195646.
Family and domain databases
Gene3D	3.30.110.60. 2 hits.
InterPro	IPR001890. RNA-binding_CRM. [Graphical view]
Pfam	PF01985. CRS1_YhbY. 2 hits. [Graphical view]
ProDom	PD010559. RNA-binding_CRM. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM01103. CRS1_YhbY. 2 hits. [Graphical view]
SUPFAM	SSF75471. CRS1_YhbY. 2 hits.
PROSITE	PS51295. CRM. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CAF1P_MAIZE

Accession

Primary (citable) accession number: Q84N49

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 3, 2007
Last sequence update:	June 1, 2003
Last modified:	April 3, 2013
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program