Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q84N49

- CAF1P_MAIZE

UniProt

Q84N49 - CAF1P_MAIZE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

CRS2-associated factor 1, chloroplastic

Gene

CAF1

Organism
Zea mays (Maize)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Required for the splicing of group IIB introns in chloroplasts. Forms splicing particles with CRS2. Interacts with RNA and confers intron specificity of the splicing particles.1 Publication

GO - Molecular functioni

  1. RNA binding Source: UniProtKB-KW

GO - Biological processi

  1. chloroplast organization Source: EnsemblPlants/Gramene
  2. Group II intron splicing Source: EnsemblPlants/Gramene
  3. mRNA processing Source: UniProtKB-KW
  4. rRNA processing Source: EnsemblPlants/Gramene
  5. tRNA metabolic process Source: EnsemblPlants/Gramene
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
CRS2-associated factor 1, chloroplastic
Alternative name(s):
Chloroplastic group IIA intron splicing facilitator CRS2-associated factor 1
Gene namesi
Name:CAF1
OrganismiZea mays (Maize)
Taxonomic identifieri4577 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogoneaeZea

Organism-specific databases

GrameneiQ84N49.
MaizeGDBi886554.

Subcellular locationi

Plastidchloroplast stroma 1 Publication

GO - Cellular componenti

  1. chloroplast stroma Source: EnsemblPlants/Gramene
  2. ribonucleoprotein complex Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 5454ChloroplastSequence AnalysisAdd
BLAST
Chaini55 – 674620CRS2-associated factor 1, chloroplasticPRO_0000283615Add
BLAST

Interactioni

Subunit structurei

Interacts with CRS2 and RNA. Part of large ribonucleo-protein complexes that include group IIB introns, CRS2 and CAF1.2 Publications

Protein-protein interaction databases

DIPiDIP-48740N.

Structurei

3D structure databases

ProteinModelPortaliQ84N49.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini183 – 27997CRM 1PROSITE-ProRule annotationAdd
BLAST
Domaini301 – 39797CRM 2PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni554 – 57623CRS2 bindingBy similarityAdd
BLAST

Sequence similaritiesi

Contains 2 CRM domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transit peptide

Phylogenomic databases

HOGENOMiHOG000195646.

Family and domain databases

Gene3Di3.30.110.60. 2 hits.
InterProiIPR001890. RNA-binding_CRM.
[Graphical view]
PfamiPF01985. CRS1_YhbY. 2 hits.
[Graphical view]
ProDomiPD010559. RNA-binding_CRM. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM01103. CRS1_YhbY. 2 hits.
[Graphical view]
SUPFAMiSSF75471. SSF75471. 2 hits.
PROSITEiPS51295. CRM. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q84N49-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MATARLPSRS FLSPAQQSYP RLPASVRLCL SHHEQPPTGP KRHRRAATSH
60 70 80 90 100
PAFSAAARGR AKKIPIADTD EPAAGVRVTD RGISYRLDGA PFEFQYSYTE
110 120 130 140 150
APRARPVALR EAPFMPFGPE ATPRPWTGRK PLPKSRKELP EFDSFVLPAP
160 170 180 190 200
GKKGVKPVQS PGPFLAGMEP RYQSVSREDI LGEPLTKEEV SELVKGSLKS
210 220 230 240 250
KRQLNMGRDG LTHNMLENIH SHWKRKRVCK IKCKGVCTID MDNICHQLEE
260 270 280 290 300
KVGGKVIHRQ GGVIFLFRGR NYNYRTRPCF PLMLWKPVAP VYPRLVTKVP
310 320 330 340 350
GGLTPDEATE MRTRGHQLPP ICKLGKNGVY ANLVNQVREA FEACDLVRVD
360 370 380 390 400
CSGLNKSDCR KIGAKLKDLV PCILLSFEFE HILMWRGSDW KSSLPPLENS
410 420 430 440 450
YEVTKVQESF SGKESNEKVT HSGNVLAQIE LVSAATSHKN WNLDEGQEKF
460 470 480 490 500
KDSTDSDMVL NSAKDVPALF HSTGISRTEP SADIPLEYSP LNPVCDIMDP
510 520 530 540 550
SLNCRSIPTN NCESRALVEK SEHCPDDYNL EMKRKRNDGT KGTVVLNSGS
560 570 580 590 600
KVEGLLCLLE QAIHSGRALV LSEDELADSD LVYEKSVAFT KSIPRRLVFE
610 620 630 640 650
NTRRKSSARR NVPDNHARTK THLVENKMLS SPVENKFIVN GGSAMQTIDH
660 670
GQEFLSDVVH QGTLRVDELA KLLA
Length:674
Mass (Da):75,145
Last modified:June 1, 2003 - v1
Checksum:i0C3454B196954E56
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY264368 mRNA. Translation: AAP22135.1.
RefSeqiNP_001105220.1. NM_001111750.1.
XP_008652328.1. XM_008654106.1.
UniGeneiZm.96493.

Genome annotation databases

GeneIDi103632293.
542118.
KEGGizma:542118.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY264368 mRNA. Translation: AAP22135.1 .
RefSeqi NP_001105220.1. NM_001111750.1.
XP_008652328.1. XM_008654106.1.
UniGenei Zm.96493.

3D structure databases

ProteinModelPortali Q84N49.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-48740N.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 103632293.
542118.
KEGGi zma:542118.

Organism-specific databases

Gramenei Q84N49.
MaizeGDBi 886554.

Phylogenomic databases

HOGENOMi HOG000195646.

Family and domain databases

Gene3Di 3.30.110.60. 2 hits.
InterProi IPR001890. RNA-binding_CRM.
[Graphical view ]
Pfami PF01985. CRS1_YhbY. 2 hits.
[Graphical view ]
ProDomi PD010559. RNA-binding_CRM. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
SMARTi SM01103. CRS1_YhbY. 2 hits.
[Graphical view ]
SUPFAMi SSF75471. SSF75471. 2 hits.
PROSITEi PS51295. CRM. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Group II intron splicing factors derived by diversification of an ancient RNA-binding domain."
    Ostheimer G.J., Williams-Carrier R., Belcher S., Osborne E., Gierke J., Barkan A.
    EMBO J. 22:3919-3929(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH CRS2.
    Strain: cv. B73.
  2. "Formation of the CRS2-CAF2 group II intron splicing complex is mediated by a 22-amino acid motif in the COOH-terminal region of CAF2."
    Ostheimer G.J., Rojas M., Hadjivassiliou H., Barkan A.
    J. Biol. Chem. 281:4732-4738(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CRS2.

Entry informationi

Entry nameiCAF1P_MAIZE
AccessioniPrimary (citable) accession number: Q84N49
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: June 1, 2003
Last modified: November 26, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3