Reviewed, UniProtKB/Swiss-Prot Q84N48 (CAF2P_MAIZE)

Names and origin

Protein names

Recommended name:
    CRS2-associated factor 2, chloroplastic
Alternative name(s):
    Chloroplastic group IIA intron splicing facilitator CRS2-associated factor 2

Gene names

Name:

CAF2

Organism

Zea mays (Maize)

Taxonomic identifier

4577 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › PACCAD clade › Panicoideae › Andropogoneae › Zea

Protein attributes

Sequence length	611 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Required for the splicing of group IIB introns in chloroplasts. Forms splicing particles with CRS2. Interacts with RNA and confers intron specificity of the splicing particles. Ref.1
Subunit structure	Interacts with CRS2 and RNA. Part of large ribonucleo-protein complexes that include group IIB introns, CRS2 and CAF2. Ref.1 Ref.2
Subcellular location	Plastid › chloroplast stroma Ref.1.
Sequence similarities	Contains 2 CRM domains.

Ontologies

Keywords
Biological process	mRNA processing mRNA splicing
Cellular component	Chloroplast Plastid
Domain	Repeat Transit peptide
Ligand	RNA-binding
Molecular function	Ribonucleoprotein
Gene Ontology (GO)
Biological process	RNA splicing Inferred from electronic annotation. Source: UniProtKB-KW mRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast stroma Inferred from electronic annotation. Source: UniProtKB-SubCell ribonucleoprotein complex Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 58	58	Chloroplast Potential
	Chain	59 – 611	553	CRS2-associated factor 2, chloroplastic		PRO_0000283620
Regions
	Domain	232 – 328	97	CRM 1
	Domain	350 – 446	97	CRM 2
	Region	486 – 509	24	CRS2 binding
	Compositional bias	2 – 165	164	Pro-rich

Sequences

	Sequence		Length	Mass (Da)
	Q84N48-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 587E44D18160AED7	FASTA	611	68,069
	10 20 30 40 50 60 MPPPPPQRPA SSHVGRANLF SASPPPLSNR RYPHHRSLPL PPVSPRRRDP KKHSQQPSQE 70 80 90 100 110 120 EPTDSGPTRT VTTNPAFRAA HLRTAYRKPV PPAAAAGEGE ALLAADPTDA ASGRAVVVGP 130 140 150 160 170 180 SGLSFRLPGA PFDFQFSYSE APRAPPLAIR EPAFLPFAPP TMPRPWTGKA PLLTKEEKAR 190 200 210 220 230 240 RRGVRLHTPL GQETPQTVSA HGIMMEVRER RKMDLARVSP GDGRSREEVL GEPLTPSEVR 250 260 270 280 290 300 ALVKPHISHN RQLNIGRDGL THNMLEMIHC HWRRQEICKV RCRGVPTVDM KNLCYHLEEK 310 320 330 340 350 360 SGGKVIHRVG GVVFLYRGRH YDPKTRPRYP LMLWKPATPV YPKLIKEAPD GFTKEEADEM 370 380 390 400 410 420 RRKGRDLLPI CKLAKNGIYI TLVKDVRDAF EGSDLVKIDC EGLNPSDYKK IGAKLRDLVP 430 440 450 460 470 480 CVLLSFDDEQ ILMHRGKEWK SRYSKPLTLI PKVPKNNLAM TSVMNSSDEV SDANTQVAIR 490 500 510 520 530 540 EVLRPKMFKL WKSAVDSSLA LLLDDAEANN LTPDSLLTLV EEFSVTSQAV EHSFPALLVT 550 560 570 580 590 600 NGDASTDSLS AEYMNDEPET SVAGNEEGQL EQSPDLRDDE HFDVDMFERL ESSVPLGSLP 610 IDSMIERLNS E

References

[1]	"Group II intron splicing factors derived by diversification of an ancient RNA-binding domain." Ostheimer G.J., Williams-Carrier R., Belcher S., Osborne E., Gierke J., Barkan A. EMBO J. 22:3919-3929(2003) [PubMed: 12881426] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH CRS2. Strain: cv. B73.
[2]	"Formation of the CRS2-CAF2 group II intron splicing complex is mediated by a 22-amino acid motif in the COOH-terminal region of CAF2." Ostheimer G.J., Rojas M., Hadjivassiliou H., Barkan A. J. Biol. Chem. 281:4732-4738(2006) [PubMed: 16379013] [Abstract] Cited for: INTERACTION WITH CRS2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY264369 mRNA. Translation: AAP22136.1.
RefSeq	NP_001105669.1.
UniGene	Zm.14985
3D structure databases
SMR	Q84N48. Positions 234-324, 352-446.
ModBase	Search...
Genome annotation databases
GeneID	542682.
KEGG	zma:542682.
Organism-specific databases
Gramene	Q84N48.
MaizeGDB	886551.
Family and domain databases
InterPro	IPR001890. RNA-binding_CRM. [Graphical view]
Gene3D	G3DSA:3.30.110.60. CRS1_YhbY. 1 hit.
Pfam	PF01985. CRS1_YhbY. 2 hits. [Graphical view]
ProDom	PD010559. RNA-binding_CRM. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS51295. CRM. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CAF2P_MAIZE

Accession

Primary (citable) accession number: Q84N48

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 3, 2007
Last sequence update:	June 1, 2003
Last modified:	January 19, 2010
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)