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Q84N48 (CAF2P_MAIZE) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 44. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
CRS2-associated factor 2, chloroplastic
Alternative name(s):
Chloroplastic group IIA intron splicing facilitator CRS2-associated factor 2
Gene names
Name:CAF2
OrganismZea mays (Maize)
Taxonomic identifier4577 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogoneaeZea

Protein attributes

Sequence length611 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for the splicing of group IIB introns in chloroplasts. Forms splicing particles with CRS2. Interacts with RNA and confers intron specificity of the splicing particles. Ref.1

Subunit structure

Interacts with CRS2 and RNA. Part of large ribonucleo-protein complexes that include group IIB introns, CRS2 and CAF2. Ref.1 Ref.2

Subcellular location

Plastidchloroplast stroma Ref.1.

Sequence similarities

Contains 2 CRM domains.

Ontologies

Keywords
   Biological processmRNA processing
mRNA splicing
   Cellular componentChloroplast
Plastid
   DomainRepeat
Transit peptide
   LigandRNA-binding
   Molecular functionRibonucleoprotein
Gene Ontology (GO)
   Biological_processGroup II intron splicing

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

mRNA processing

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentchloroplast stroma

Inferred from electronic annotation. Source: UniProtKB-SubCell

ribonucleoprotein complex

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionRNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 5858Chloroplast Potential
Chain59 – 611553CRS2-associated factor 2, chloroplastic
PRO_0000283620

Regions

Domain232 – 32897CRM 1
Domain350 – 44697CRM 2
Region486 – 50924CRS2 binding
Compositional bias2 – 165164Pro-rich

Sequences

Sequence LengthMass (Da)Tools
Q84N48 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 587E44D18160AED7

FASTA61168,069
        10         20         30         40         50         60 
MPPPPPQRPA SSHVGRANLF SASPPPLSNR RYPHHRSLPL PPVSPRRRDP KKHSQQPSQE 

        70         80         90        100        110        120 
EPTDSGPTRT VTTNPAFRAA HLRTAYRKPV PPAAAAGEGE ALLAADPTDA ASGRAVVVGP 

       130        140        150        160        170        180 
SGLSFRLPGA PFDFQFSYSE APRAPPLAIR EPAFLPFAPP TMPRPWTGKA PLLTKEEKAR 

       190        200        210        220        230        240 
RRGVRLHTPL GQETPQTVSA HGIMMEVRER RKMDLARVSP GDGRSREEVL GEPLTPSEVR 

       250        260        270        280        290        300 
ALVKPHISHN RQLNIGRDGL THNMLEMIHC HWRRQEICKV RCRGVPTVDM KNLCYHLEEK 

       310        320        330        340        350        360 
SGGKVIHRVG GVVFLYRGRH YDPKTRPRYP LMLWKPATPV YPKLIKEAPD GFTKEEADEM 

       370        380        390        400        410        420 
RRKGRDLLPI CKLAKNGIYI TLVKDVRDAF EGSDLVKIDC EGLNPSDYKK IGAKLRDLVP 

       430        440        450        460        470        480 
CVLLSFDDEQ ILMHRGKEWK SRYSKPLTLI PKVPKNNLAM TSVMNSSDEV SDANTQVAIR 

       490        500        510        520        530        540 
EVLRPKMFKL WKSAVDSSLA LLLDDAEANN LTPDSLLTLV EEFSVTSQAV EHSFPALLVT 

       550        560        570        580        590        600 
NGDASTDSLS AEYMNDEPET SVAGNEEGQL EQSPDLRDDE HFDVDMFERL ESSVPLGSLP 

       610 
IDSMIERLNS E 

« Hide

References

[1]"Group II intron splicing factors derived by diversification of an ancient RNA-binding domain."
Ostheimer G.J., Williams-Carrier R., Belcher S., Osborne E., Gierke J., Barkan A.
EMBO J. 22:3919-3929(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH CRS2.
Strain: cv. B73.
[2]"Formation of the CRS2-CAF2 group II intron splicing complex is mediated by a 22-amino acid motif in the COOH-terminal region of CAF2."
Ostheimer G.J., Rojas M., Hadjivassiliou H., Barkan A.
J. Biol. Chem. 281:4732-4738(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH CRS2.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY264369 mRNA. Translation: AAP22136.1.
RefSeqNP_001105669.1. NM_001112199.1.
UniGeneZm.14985.

3D structure databases

ProteinModelPortalQ84N48.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-48741N.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID542682.
KEGGzma:542682.

Organism-specific databases

GrameneQ84N48.
MaizeGDB886551.

Phylogenomic databases

HOGENOMHOG000237818.
OMARRQEICK.

Family and domain databases

Gene3D3.30.110.60. 2 hits.
InterProIPR001890. RNA-binding_CRM.
[Graphical view]
PfamPF01985. CRS1_YhbY. 2 hits.
[Graphical view]
ProDomPD010559. RNA-binding_CRM. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM01103. CRS1_YhbY. 2 hits.
[Graphical view]
SUPFAMSSF75471. SSF75471. 2 hits.
PROSITEPS51295. CRM. 2 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCAF2P_MAIZE
AccessionPrimary (citable) accession number: Q84N48
Entry history
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: June 1, 2003
Last modified: October 16, 2013
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families