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Protein

CRS2-associated factor 2, chloroplastic

Gene

CAF2

Organism
Zea mays (Maize)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Required for the splicing of group IIB introns in chloroplasts. Forms splicing particles with CRS2. Interacts with RNA and confers intron specificity of the splicing particles.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
CRS2-associated factor 2, chloroplastic
Alternative name(s):
Chloroplastic group IIA intron splicing facilitator CRS2-associated factor 2
Gene namesi
Name:CAF2
OrganismiZea mays (Maize)
Taxonomic identifieri4577 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogonodaeAndropogoneaeTripsacinaeZea
Proteomesi
  • UP000007305 Componenti: Unplaced

Organism-specific databases

MaizeGDBi886551.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 58ChloroplastSequence analysisAdd BLAST58
ChainiPRO_000028362059 – 611CRS2-associated factor 2, chloroplasticAdd BLAST553

Proteomic databases

PaxDbiQ84N48.

Expressioni

Gene expression databases

GenevisibleiQ84N48. ZM.

Interactioni

Subunit structurei

Interacts with CRS2 and RNA. Part of large ribonucleo-protein complexes that include group IIB introns, CRS2 and CAF2.2 Publications

Protein-protein interaction databases

BioGridi951897. 1 interactor.
DIPiDIP-48741N.
STRINGi4577.AC199526.5_FGP003.

Structurei

3D structure databases

ProteinModelPortaliQ84N48.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini232 – 328CRM 1PROSITE-ProRule annotationAdd BLAST97
Domaini350 – 446CRM 2PROSITE-ProRule annotationAdd BLAST97

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni486 – 509CRS2 bindingAdd BLAST24

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi2 – 165Pro-richAdd BLAST164

Sequence similaritiesi

Contains 2 CRM domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transit peptide

Phylogenomic databases

eggNOGiENOG410IEAA. Eukaryota.
ENOG410XQZ0. LUCA.
HOGENOMiHOG000237818.
OMAiYSECPRA.
OrthoDBiEOG093607WA.

Family and domain databases

Gene3Di3.30.110.60. 2 hits.
InterProiIPR001890. RNA-binding_CRM.
[Graphical view]
PfamiPF01985. CRS1_YhbY. 2 hits.
[Graphical view]
ProDomiPD010559. RNA-binding_CRM. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM01103. CRS1_YhbY. 2 hits.
[Graphical view]
SUPFAMiSSF75471. SSF75471. 2 hits.
PROSITEiPS51295. CRM. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q84N48-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPPPPPQRPA SSHVGRANLF SASPPPLSNR RYPHHRSLPL PPVSPRRRDP
60 70 80 90 100
KKHSQQPSQE EPTDSGPTRT VTTNPAFRAA HLRTAYRKPV PPAAAAGEGE
110 120 130 140 150
ALLAADPTDA ASGRAVVVGP SGLSFRLPGA PFDFQFSYSE APRAPPLAIR
160 170 180 190 200
EPAFLPFAPP TMPRPWTGKA PLLTKEEKAR RRGVRLHTPL GQETPQTVSA
210 220 230 240 250
HGIMMEVRER RKMDLARVSP GDGRSREEVL GEPLTPSEVR ALVKPHISHN
260 270 280 290 300
RQLNIGRDGL THNMLEMIHC HWRRQEICKV RCRGVPTVDM KNLCYHLEEK
310 320 330 340 350
SGGKVIHRVG GVVFLYRGRH YDPKTRPRYP LMLWKPATPV YPKLIKEAPD
360 370 380 390 400
GFTKEEADEM RRKGRDLLPI CKLAKNGIYI TLVKDVRDAF EGSDLVKIDC
410 420 430 440 450
EGLNPSDYKK IGAKLRDLVP CVLLSFDDEQ ILMHRGKEWK SRYSKPLTLI
460 470 480 490 500
PKVPKNNLAM TSVMNSSDEV SDANTQVAIR EVLRPKMFKL WKSAVDSSLA
510 520 530 540 550
LLLDDAEANN LTPDSLLTLV EEFSVTSQAV EHSFPALLVT NGDASTDSLS
560 570 580 590 600
AEYMNDEPET SVAGNEEGQL EQSPDLRDDE HFDVDMFERL ESSVPLGSLP
610
IDSMIERLNS E
Length:611
Mass (Da):68,069
Last modified:June 1, 2003 - v1
Checksum:i587E44D18160AED7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY264369 mRNA. Translation: AAP22136.1.
RefSeqiNP_001105669.1. NM_001112199.1.
UniGeneiZm.14985.

Genome annotation databases

EnsemblPlantsiZm00001d040696_T003; Zm00001d040696_T003; Zm00001d040696.
GeneIDi542682.
GrameneiZm00001d040696_T003; Zm00001d040696_T003; Zm00001d040696.
KEGGizma:542682.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY264369 mRNA. Translation: AAP22136.1.
RefSeqiNP_001105669.1. NM_001112199.1.
UniGeneiZm.14985.

3D structure databases

ProteinModelPortaliQ84N48.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi951897. 1 interactor.
DIPiDIP-48741N.
STRINGi4577.AC199526.5_FGP003.

Proteomic databases

PaxDbiQ84N48.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiZm00001d040696_T003; Zm00001d040696_T003; Zm00001d040696.
GeneIDi542682.
GrameneiZm00001d040696_T003; Zm00001d040696_T003; Zm00001d040696.
KEGGizma:542682.

Organism-specific databases

MaizeGDBi886551.

Phylogenomic databases

eggNOGiENOG410IEAA. Eukaryota.
ENOG410XQZ0. LUCA.
HOGENOMiHOG000237818.
OMAiYSECPRA.
OrthoDBiEOG093607WA.

Gene expression databases

GenevisibleiQ84N48. ZM.

Family and domain databases

Gene3Di3.30.110.60. 2 hits.
InterProiIPR001890. RNA-binding_CRM.
[Graphical view]
PfamiPF01985. CRS1_YhbY. 2 hits.
[Graphical view]
ProDomiPD010559. RNA-binding_CRM. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM01103. CRS1_YhbY. 2 hits.
[Graphical view]
SUPFAMiSSF75471. SSF75471. 2 hits.
PROSITEiPS51295. CRM. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCAF2P_MAIZE
AccessioniPrimary (citable) accession number: Q84N48
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: June 1, 2003
Last modified: November 30, 2016
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.