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Protein

NifU-like protein 1, chloroplastic

Gene

NIFU1

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Molecular scaffold for [Fe-S] cluster assembly of chloroplastic iron-sulfur proteins.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
NifU-like protein 1, chloroplastic
Alternative name(s):
OsNifu1
Gene namesi
Name:NIFU1
Ordered Locus Names:Os12g0176200, LOC_Os12g07700
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryza
Proteomesi
  • UP000059680 Componenti: Chromosome 12

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 7676ChloroplastSequence analysisAdd
BLAST
Chaini77 – 226150NifU-like protein 1, chloroplasticPRO_0000247614Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi116 – 116Interchain (with C-119)By similarity
Disulfide bondi119 – 119Interchain (with C-116)By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiQ84LK7.

Expressioni

Gene expression databases

ExpressionAtlasiQ84LK7. baseline and differential.
GenevisibleiQ84LK7. OS.

Interactioni

Subunit structurei

Homodimer; disulfide-linked.By similarity

Protein-protein interaction databases

STRINGi39947.LOC_Os12g07700.1.

Structurei

Secondary structure

1
226
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi73 – 753Combined sources
Helixi79 – 846Combined sources
Turni85 – 873Combined sources
Helixi88 – 914Combined sources
Turni92 – 954Combined sources
Beta strandi98 – 1036Combined sources
Beta strandi105 – 1128Combined sources
Beta strandi116 – 1194Combined sources
Helixi122 – 13514Combined sources
Beta strandi143 – 1453Combined sources
Beta strandi147 – 1493Combined sources
Helixi158 – 1658Combined sources
Turni166 – 1683Combined sources
Helixi169 – 1724Combined sources
Turni173 – 1764Combined sources
Beta strandi184 – 1863Combined sources
Beta strandi189 – 1924Combined sources
Beta strandi196 – 2038Combined sources
Helixi204 – 21512Combined sources
Beta strandi220 – 2245Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1TH5NMR-A154-226[»]
2JNVNMR-A73-153[»]
ProteinModelPortaliQ84LK7.
SMRiQ84LK7. Positions 73-226.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ84LK7.

Family & Domainsi

Sequence similaritiesi

Belongs to the NifU family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2358. Eukaryota.
COG0694. LUCA.
HOGENOMiHOG000006174.
InParanoidiQ84LK7.
OMAiMQTTTVP.

Family and domain databases

InterProiIPR001075. NIF_FeS_clus_asmbl_NifU_C.
[Graphical view]
PfamiPF01106. NifU. 2 hits.
[Graphical view]
ProDomiPD002830. NIF_FeS_clus_asmbl_NifU_C. 2 hits.
[Graphical view] [Entries sharing at least one domain]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q84LK7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQTTTVPMAA AAAVAPSTTT SSSASFKVAA YAWSSCRSSS SPATRLVAAP
60 70 80 90 100
NHQRPPLVVG AIAGLDPVTA VQLPLTAGNV ESVLDQVRPY LTADGGDVAL
110 120 130 140 150
HEIAGNVVRL KLQGACGSCP SSLITIKRGI ERRLMEKIPD VAAVEPVTDK
160 170 180 190 200
ETGLELNEEN VEKVLNEIRP YLAGTGGGGL QFLMIKGPIV KVRLTGPAAV
210 220
VRTVRIAVSK KLREKIPSIQ IVQLLS
Length:226
Mass (Da):23,653
Last modified:June 1, 2003 - v1
Checksum:iADD9AB2D2D540DD5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB096013 mRNA. Translation: BAC76603.1.
DP000011 Genomic DNA. Translation: ABA96603.1.
AP008218 Genomic DNA. Translation: BAF29313.1.
AP014968 Genomic DNA. Translation: BAT16109.1.
RefSeqiXP_015619438.1. XM_015763952.1.
UniGeneiOs.5621.

Genome annotation databases

EnsemblPlantsiOS12T0176200-01; OS12T0176200-01; OS12G0176200.
GeneIDi4351651.
GrameneiOS12T0176200-01; OS12T0176200-01; OS12G0176200.
KEGGiosa:4351651.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB096013 mRNA. Translation: BAC76603.1.
DP000011 Genomic DNA. Translation: ABA96603.1.
AP008218 Genomic DNA. Translation: BAF29313.1.
AP014968 Genomic DNA. Translation: BAT16109.1.
RefSeqiXP_015619438.1. XM_015763952.1.
UniGeneiOs.5621.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1TH5NMR-A154-226[»]
2JNVNMR-A73-153[»]
ProteinModelPortaliQ84LK7.
SMRiQ84LK7. Positions 73-226.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi39947.LOC_Os12g07700.1.

Proteomic databases

PaxDbiQ84LK7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiOS12T0176200-01; OS12T0176200-01; OS12G0176200.
GeneIDi4351651.
GrameneiOS12T0176200-01; OS12T0176200-01; OS12G0176200.
KEGGiosa:4351651.

Phylogenomic databases

eggNOGiKOG2358. Eukaryota.
COG0694. LUCA.
HOGENOMiHOG000006174.
InParanoidiQ84LK7.
OMAiMQTTTVP.

Miscellaneous databases

EvolutionaryTraceiQ84LK7.

Gene expression databases

ExpressionAtlasiQ84LK7. baseline and differential.
GenevisibleiQ84LK7. OS.

Family and domain databases

InterProiIPR001075. NIF_FeS_clus_asmbl_NifU_C.
[Graphical view]
PfamiPF01106. NifU. 2 hits.
[Graphical view]
ProDomiPD002830. NIF_FeS_clus_asmbl_NifU_C. 2 hits.
[Graphical view] [Entries sharing at least one domain]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nuclear-encoded plastid gene, NifU1, in rice."
    Asayama M.
    Submitted (NOV-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Nipponbare.
    Tissue: Shoot.
  2. "The sequence of rice chromosomes 11 and 12, rich in disease resistance genes and recent gene duplications."
    The rice chromosomes 11 and 12 sequencing consortia
    BMC Biol. 3:20-20(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  4. "The rice annotation project database (RAP-DB): 2008 update."
    The rice annotation project (RAP)
    Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: cv. Nipponbare.
  5. Cited for: GENOME REANNOTATION.
    Strain: cv. Nipponbare.
  6. "Refolding and purification of recombinant OsNifU1A domain II that was expressed by Escherichia coli."
    Katoh S., Murata K., Kubota Y., Kumeta H., Ogura K., Inagaki F., Asayama M., Katoh E.
    Protein Expr. Purif. 43:149-156(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: PURIFICATION OF RECOMBINANT PROTEIN.
  7. "Solution structure of C-terminal domain of nifU-like protein from Oryza sativa."
    Kumeta H., Ogura K., Asayama M., Katoh S., Katoh E., Inagaki F.
    Submitted (JUL-2005) to the PDB data bank
    Cited for: STRUCTURE BY NMR OF 154-226.

Entry informationi

Entry nameiNIFU1_ORYSJ
AccessioniPrimary (citable) accession number: Q84LK7
Secondary accession number(s): Q0IPQ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: June 1, 2003
Last modified: June 8, 2016
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.