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Protein

Probable ubiquitin receptor RAD23a

Gene

RAD23A

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May be involved in nucleotide excision repair (By similarity). Binds and presumably selects ubiquitin-conjugates for destruction. Prefers multiubiquitin chains rather than single ubiquitins, with a binding affinity for 'Lys-48'-linked ubiquitin chains. Acts as a ubiquitin receptor that associates with the 26S proteasomal docking subunit RPN10 for the indirect recognition of ubiquitinated substrates of ubiquitin/26S proteasome-mediated proteolysis (UPP) (By similarity).By similarity

GO - Molecular functioni

  • damaged DNA binding Source: InterPro
  • polyubiquitin binding Source: UniProtKB
  • proteasome binding Source: TAIR
  • ubiquitin binding Source: TAIR

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA repair

Enzyme and pathway databases

ReactomeiR-ATH-532668. N-glycan trimming in the ER and Calnexin/Calreticulin cycle.
R-ATH-5689877. Josephin domain DUBs.
R-ATH-5696394. DNA Damage Recognition in GG-NER.
R-ATH-5696395. Formation of Incision Complex in GG-NER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable ubiquitin receptor RAD23a
Short name:
AtRAD23a
Alternative name(s):
Putative DNA repair protein RAD23-2
RAD23-like protein 2
Short name:
AtRAD23-2
Gene namesi
Name:RAD23A
Synonyms:RAD23, RAD23-2
Ordered Locus Names:At1g16190
ORF Names:F3O9.1
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G16190.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • nucleus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001149091 – 368Probable ubiquitin receptor RAD23aAdd BLAST368

Proteomic databases

PaxDbiQ84L32.

Expressioni

Tissue specificityi

Widely expressed in the whole plant.1 Publication

Gene expression databases

GenevisibleiQ84L32. AT.

Interactioni

Subunit structurei

Interacts with 'Lys-48'-linked polyubiquitin chains. Interacts with RPN10.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
PSMD4P550363EBI-6394924,EBI-359318From a different organism.
RPN10P550343EBI-6394924,EBI-2620423

GO - Molecular functioni

  • polyubiquitin binding Source: UniProtKB
  • proteasome binding Source: TAIR
  • ubiquitin binding Source: TAIR

Protein-protein interaction databases

IntActiQ84L32. 5 interactors.
MINTiMINT-7308125.
STRINGi3702.AT1G16190.1.

Structurei

3D structure databases

ProteinModelPortaliQ84L32.
SMRiQ84L32.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 77Ubiquitin-likePROSITE-ProRule annotationAdd BLAST77
Domaini142 – 185UBA 1PROSITE-ProRule annotationAdd BLAST44
Domaini239 – 282STI1Add BLAST44
Domaini320 – 360UBA 2PROSITE-ProRule annotationAdd BLAST41

Sequence similaritiesi

Belongs to the RAD23 family.Curated
Contains 1 STI1 domain.Curated
Contains 2 UBA domains.PROSITE-ProRule annotation
Contains 1 ubiquitin-like domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0011. Eukaryota.
COG5272. LUCA.
HOGENOMiHOG000172162.
InParanoidiQ84L32.
KOiK10839.
OMAiGEESPQM.
OrthoDBiEOG09360ED8.
PhylomeDBiQ84L32.

Family and domain databases

Gene3Di1.10.10.540. 1 hit.
InterProiIPR004806. Rad23.
IPR006636. STI1_HS-bd.
IPR015940. UBA.
IPR009060. UBA-like.
IPR029071. Ubiquitin-rel_dom.
IPR000626. Ubiquitin_dom.
IPR015360. XPC-bd.
[Graphical view]
PfamiPF00627. UBA. 2 hits.
PF00240. ubiquitin. 1 hit.
PF09280. XPC-binding. 1 hit.
[Graphical view]
PRINTSiPR01839. RAD23PROTEIN.
SMARTiSM00727. STI1. 1 hit.
SM00165. UBA. 2 hits.
SM00213. UBQ. 1 hit.
[Graphical view]
SUPFAMiSSF101238. SSF101238. 1 hit.
SSF46934. SSF46934. 2 hits.
SSF54236. SSF54236. 1 hit.
TIGRFAMsiTIGR00601. rad23. 1 hit.
PROSITEiPS50030. UBA. 2 hits.
PS50053. UBIQUITIN_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q84L32-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKLTVKTLKG SHFEIRVLPT DTIMAVKKNI EDSQSKDNYP CGQQLLIHNG
60 70 80 90 100
KVLKDETTLV ENKVTEEGFL VVMLSKSKTA SSAGPSSTQP TSTTTSTISS
110 120 130 140 150
TTLAAPSTTQ SIAVPASNST PVQEQPTAQS DTYGQAASTL VSGSSIEQMV
160 170 180 190 200
QQIMEMGGGS WDKETVTRAL RAAYNNPERA VDYLYSGIPE TVTIPATNLS
210 220 230 240 250
GVGSGRELTA PPPSGGPNSS PLDLFPQEAV SDAAGGDLGT LEFLRGNDQF
260 270 280 290 300
QQLRSMVNSN PQILQPMLQE LGKQNPQLLR LIQENQAEFL QLLNEPYEGS
310 320 330 340 350
DGDVDIFDQP DQEMPHSVNV TPEEQESIER LEAMGFDRAI VIEAFLSCDR
360
NEELAANYLL EHSADFED
Length:368
Mass (Da):39,842
Last modified:August 16, 2005 - v2
Checksum:iBFBF4FE75E6C431F
GO
Isoform 2 (identifier: Q84L32-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     20-21: Missing.

Note: May be due to a competing donor splice site. No experimental confirmation available.
Show »
Length:366
Mass (Da):39,626
Checksum:i5ECA2D1604B2B001
GO

Sequence cautioni

The sequence AAD34676 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAF18513 differs from that shown. Reason: Erroneous gene model prediction.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_01497920 – 21Missing in isoform 2. 1 Publication2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB109195 mRNA. Translation: BAC76391.1.
AC006341 Genomic DNA. Translation: AAD34676.1. Sequence problems.
AC010924 Genomic DNA. Translation: AAF18513.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE29418.1.
PIRiG86296.
H86296.
RefSeqiNP_173070.1. NM_101486.3. [Q84L32-1]
UniGeneiAt.22124.

Genome annotation databases

EnsemblPlantsiAT1G16190.1; AT1G16190.1; AT1G16190. [Q84L32-1]
GeneIDi838188.
GrameneiAT1G16190.1; AT1G16190.1; AT1G16190.
KEGGiath:AT1G16190.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB109195 mRNA. Translation: BAC76391.1.
AC006341 Genomic DNA. Translation: AAD34676.1. Sequence problems.
AC010924 Genomic DNA. Translation: AAF18513.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE29418.1.
PIRiG86296.
H86296.
RefSeqiNP_173070.1. NM_101486.3. [Q84L32-1]
UniGeneiAt.22124.

3D structure databases

ProteinModelPortaliQ84L32.
SMRiQ84L32.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ84L32. 5 interactors.
MINTiMINT-7308125.
STRINGi3702.AT1G16190.1.

Proteomic databases

PaxDbiQ84L32.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G16190.1; AT1G16190.1; AT1G16190. [Q84L32-1]
GeneIDi838188.
GrameneiAT1G16190.1; AT1G16190.1; AT1G16190.
KEGGiath:AT1G16190.

Organism-specific databases

TAIRiAT1G16190.

Phylogenomic databases

eggNOGiKOG0011. Eukaryota.
COG5272. LUCA.
HOGENOMiHOG000172162.
InParanoidiQ84L32.
KOiK10839.
OMAiGEESPQM.
OrthoDBiEOG09360ED8.
PhylomeDBiQ84L32.

Enzyme and pathway databases

ReactomeiR-ATH-532668. N-glycan trimming in the ER and Calnexin/Calreticulin cycle.
R-ATH-5689877. Josephin domain DUBs.
R-ATH-5696394. DNA Damage Recognition in GG-NER.
R-ATH-5696395. Formation of Incision Complex in GG-NER.

Miscellaneous databases

PROiQ84L32.

Gene expression databases

GenevisibleiQ84L32. AT.

Family and domain databases

Gene3Di1.10.10.540. 1 hit.
InterProiIPR004806. Rad23.
IPR006636. STI1_HS-bd.
IPR015940. UBA.
IPR009060. UBA-like.
IPR029071. Ubiquitin-rel_dom.
IPR000626. Ubiquitin_dom.
IPR015360. XPC-bd.
[Graphical view]
PfamiPF00627. UBA. 2 hits.
PF00240. ubiquitin. 1 hit.
PF09280. XPC-binding. 1 hit.
[Graphical view]
PRINTSiPR01839. RAD23PROTEIN.
SMARTiSM00727. STI1. 1 hit.
SM00165. UBA. 2 hits.
SM00213. UBQ. 1 hit.
[Graphical view]
SUPFAMiSSF101238. SSF101238. 1 hit.
SSF46934. SSF46934. 2 hits.
SSF54236. SSF54236. 1 hit.
TIGRFAMsiTIGR00601. rad23. 1 hit.
PROSITEiPS50030. UBA. 2 hits.
PS50053. UBIQUITIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRD23A_ARATH
AccessioniPrimary (citable) accession number: Q84L32
Secondary accession number(s): Q9S9L8, Q9SA20
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 23, 2004
Last sequence update: August 16, 2005
Last modified: November 30, 2016
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.