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Q84KP0

- DFRA_PYRCO

UniProt

Q84KP0 - DFRA_PYRCO

Protein

Bifunctional dihydroflavonol 4-reductase/flavanone 4-reductase

Gene

DFR

Organism
Pyrus communis (Pear) (Pyrus domestica)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 41 (01 Oct 2014)
      Sequence version 1 (01 Jun 2003)
      Previous versions | rss
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    Functioni

    Bifunctional enzyme involved in the flavonoid metabolism. May use dihydroquercetin, eriodictyol, garbanzol (5-deoxydihydrokaempferol), dihydrofisetin (5-deoxydihydroquercetin), dihydrokaempferol to a low extent (5%), but not naringenin, 5-deoxynaringenin or butin (5-deoxyeriodictyol) as substrate.

    Catalytic activityi

    (2S)-flavan-4-ol + NADP+ = (2S)-flavanone + NADPH.
    Cis-3,4-leucopelargonidin + NADP+ = (+)-dihydrokaempferol + NADPH.

    Kineticsi

    1. KM=3.8 µM for eriodictyol1 Publication
    2. KM=3.0 µM for dihydroquercetin1 Publication

    Vmax=1.6 nmol/sec/g enzyme toward eriodictyol1 Publication

    Vmax=4.7 nmol/sec/g enzyme toward dihydroquercetin1 Publication

    pH dependencei

    Optimum pH is 5.75 with dihydroquercetin as substrate.1 Publication

    GO - Molecular functioni

    1. coenzyme binding Source: InterPro
    2. dihydrokaempferol 4-reductase activity Source: UniProtKB-EC
    3. flavanone 4-reductase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cellular metabolic process Source: InterPro
    2. flavonoid biosynthetic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Flavonoid biosynthesis

    Keywords - Ligandi

    NADP

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bifunctional dihydroflavonol 4-reductase/flavanone 4-reductase
    Alternative name(s):
    Dihydroflavonol 4-reductase (EC:1.1.1.219)
    Short name:
    DFR
    Flavanone 4-reductase (EC:1.1.1.234)
    Short name:
    FNR
    Gene namesi
    Name:DFR
    OrganismiPyrus communis (Pear) (Pyrus domestica)
    Taxonomic identifieri23211 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsRosalesRosaceaeMaloideaeMaleaePyrus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 347347Bifunctional dihydroflavonol 4-reductase/flavanone 4-reductasePRO_0000367057Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliQ84KP0.
    SMRiQ84KP0. Positions 5-326.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR001509. Epimerase_deHydtase.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PfamiPF01370. Epimerase. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q84KP0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGSESESVCV TGASGFIGSW LVMRLLEHGY TVRATVRDPT NQKKVKHLLD    50
    LPKAETHLTL WKADLADEGS FDEAIQGCSG VFHVATPMDF ESRDPENEVI 100
    KPTINGLLDI LKACQKAKTV RKLVFTSSAG TVNVEEHQKP VYDESNWSDV 150
    EFCRSVKMTG WMYFVSKTLA EQAAWKYAKE NNIDFITIIP TLVIGPFLMP 200
    SMPPSLITGL SPILRNESHY GIIKQGQYVH LDDLCLSHIY LYKHPKAEGR 250
    YICSSHDATI HELVKMLREK YPEYNIPTKF KGIDDNLEPV HFSSKKLREI 300
    GFEFKYSLED MFVGAVDACR AKGLIPIPAE KTEAAEESNL VDVKVGS 347
    Length:347
    Mass (Da):38,934
    Last modified:June 1, 2003 - v1
    Checksum:i048D1FE898418E9E
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti93 – 931R → K in strain: cv. Conference and cv. Pyrodwarf.
    Natural varianti347 – 3471S → G in strain: cv. Conference and cv. Pyrodwarf.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY227730 mRNA. Translation: AAO39818.1.
    AY227731 mRNA. Translation: AAO39819.1.
    AY227732 mRNA. Translation: AAO39820.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY227730 mRNA. Translation: AAO39818.1 .
    AY227731 mRNA. Translation: AAO39819.1 .
    AY227732 mRNA. Translation: AAO39820.1 .

    3D structure databases

    ProteinModelPortali Q84KP0.
    SMRi Q84KP0. Positions 5-326.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    InterProi IPR001509. Epimerase_deHydtase.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    Pfami PF01370. Epimerase. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning, substrate specificity of the functionally expressed dihydroflavonol 4-reductases from Malus domestica and Pyrus communis cultivars and the consequences for flavonoid metabolism."
      Fischer T.C., Halbwirth H., Meisel B., Stich K., Forkmann G.
      Arch. Biochem. Biophys. 412:223-230(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], BIOPHYSICOCHEMICAL PROPERTIES.
      Strain: cv. Abbe Fetel, cv. Conference and cv. Pyrodwarf.

    Entry informationi

    Entry nameiDFRA_PYRCO
    AccessioniPrimary (citable) accession number: Q84KP0
    Secondary accession number(s): Q84K59
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 24, 2009
    Last sequence update: June 1, 2003
    Last modified: October 1, 2014
    This is version 41 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3