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Q84KP0

- DFRA_PYRCO

UniProt

Q84KP0 - DFRA_PYRCO

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Protein

Bifunctional dihydroflavonol 4-reductase/flavanone 4-reductase

Gene

DFR

Organism
Pyrus communis (Pear) (Pyrus domestica)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Bifunctional enzyme involved in the flavonoid metabolism. May use dihydroquercetin, eriodictyol, garbanzol (5-deoxydihydrokaempferol), dihydrofisetin (5-deoxydihydroquercetin), dihydrokaempferol to a low extent (5%), but not naringenin, 5-deoxynaringenin or butin (5-deoxyeriodictyol) as substrate.

Catalytic activityi

(2S)-flavan-4-ol + NADP+ = (2S)-flavanone + NADPH.
Cis-3,4-leucopelargonidin + NADP+ = (+)-dihydrokaempferol + NADPH.

Kineticsi

  1. KM=3.8 µM for eriodictyol1 Publication
  2. KM=3.0 µM for dihydroquercetin1 Publication

Vmax=1.6 nmol/sec/g enzyme toward eriodictyol1 Publication

Vmax=4.7 nmol/sec/g enzyme toward dihydroquercetin1 Publication

pH dependencei

Optimum pH is 5.75 with dihydroquercetin as substrate.1 Publication

GO - Molecular functioni

  1. coenzyme binding Source: InterPro
  2. dihydrokaempferol 4-reductase activity Source: UniProtKB-EC
  3. flavanone 4-reductase activity Source: UniProtKB-EC

GO - Biological processi

  1. flavonoid biosynthetic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Flavonoid biosynthesis

Keywords - Ligandi

NADP

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional dihydroflavonol 4-reductase/flavanone 4-reductase
Alternative name(s):
Dihydroflavonol 4-reductase (EC:1.1.1.219)
Short name:
DFR
Flavanone 4-reductase (EC:1.1.1.234)
Short name:
FNR
Gene namesi
Name:DFR
OrganismiPyrus communis (Pear) (Pyrus domestica)
Taxonomic identifieri23211 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsRosalesRosaceaeMaloideaeMaleaePyrus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 347347Bifunctional dihydroflavonol 4-reductase/flavanone 4-reductasePRO_0000367057Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ84KP0.
SMRiQ84KP0. Positions 5-326.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR001509. Epimerase_deHydtase_N.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF01370. Epimerase. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q84KP0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGSESESVCV TGASGFIGSW LVMRLLEHGY TVRATVRDPT NQKKVKHLLD
60 70 80 90 100
LPKAETHLTL WKADLADEGS FDEAIQGCSG VFHVATPMDF ESRDPENEVI
110 120 130 140 150
KPTINGLLDI LKACQKAKTV RKLVFTSSAG TVNVEEHQKP VYDESNWSDV
160 170 180 190 200
EFCRSVKMTG WMYFVSKTLA EQAAWKYAKE NNIDFITIIP TLVIGPFLMP
210 220 230 240 250
SMPPSLITGL SPILRNESHY GIIKQGQYVH LDDLCLSHIY LYKHPKAEGR
260 270 280 290 300
YICSSHDATI HELVKMLREK YPEYNIPTKF KGIDDNLEPV HFSSKKLREI
310 320 330 340
GFEFKYSLED MFVGAVDACR AKGLIPIPAE KTEAAEESNL VDVKVGS
Length:347
Mass (Da):38,934
Last modified:June 1, 2003 - v1
Checksum:i048D1FE898418E9E
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti93 – 931R → K in strain: cv. Conference and cv. Pyrodwarf.
Natural varianti347 – 3471S → G in strain: cv. Conference and cv. Pyrodwarf.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY227730 mRNA. Translation: AAO39818.1.
AY227731 mRNA. Translation: AAO39819.1.
AY227732 mRNA. Translation: AAO39820.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY227730 mRNA. Translation: AAO39818.1 .
AY227731 mRNA. Translation: AAO39819.1 .
AY227732 mRNA. Translation: AAO39820.1 .

3D structure databases

ProteinModelPortali Q84KP0.
SMRi Q84KP0. Positions 5-326.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
InterProi IPR001509. Epimerase_deHydtase_N.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
Pfami PF01370. Epimerase. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning, substrate specificity of the functionally expressed dihydroflavonol 4-reductases from Malus domestica and Pyrus communis cultivars and the consequences for flavonoid metabolism."
    Fischer T.C., Halbwirth H., Meisel B., Stich K., Forkmann G.
    Arch. Biochem. Biophys. 412:223-230(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], BIOPHYSICOCHEMICAL PROPERTIES.
    Strain: cv. Abbe Fetel, cv. Conference and cv. Pyrodwarf.

Entry informationi

Entry nameiDFRA_PYRCO
AccessioniPrimary (citable) accession number: Q84KP0
Secondary accession number(s): Q84K59
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: June 1, 2003
Last modified: October 29, 2014
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3