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Protein

Taxoid 14-beta-hydroxylase

Gene
N/A
Organism
Taxus cuspidata (Japanese yew)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of 5-alpha-acetoxy-10beta-ol to 5-alpha-acetoxy-10beta,14beta-dihydroxy taxadiene. Also acts on taxa-4(20),11-dien-5-alpha-yl acetate.1 Publication

Catalytic activityi

10-beta-hydroxytaxa-4(20),11-dien-5-alpha-yl acetate + O2 + NADPH = 10-beta,14-beta-dihydroxytaxa-4(20),11-dien-5?-yl acetate + NADP+ + H2O.1 Publication

Kineticsi

  1. KM=33 µM for 5-alpha-acetoxy taxadiene1 Publication
  2. KM=55 µM for 5-alpha-acetoxy-10-beta-hydroxy taxadiene1 Publication

    pH dependencei

    Optimum pH is 7.5.1 Publication

    Pathwayi: taxol biosynthesis

    This protein is involved in the pathway taxol biosynthesis, which is part of Alkaloid biosynthesis.
    View all proteins of this organism that are known to be involved in the pathway taxol biosynthesis and in Alkaloid biosynthesis.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi443 – 4431Iron (heme axial ligand)By similarity

    GO - Molecular functioni

    • heme binding Source: InterPro
    • iron ion binding Source: InterPro
    • taxoid 14-beta-hydroxylase activity Source: UniProtKB

    GO - Biological processi

    • oxidation-reduction process Source: UniProtKB
    • paclitaxel biosynthetic process Source: UniProtKB-UniPathway
    • paclitaxel metabolic process Source: UniProtKB
    Complete GO annotation...

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Biological processi

    Taxol biosynthesis

    Keywords - Ligandi

    Heme, Iron, Metal-binding, NADP

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-13409.
    BRENDAi1.14.13.146. 6225.
    UniPathwayiUPA00842.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Taxoid 14-beta-hydroxylase (EC:1.14.13.146)
    Alternative name(s):
    Taxane 14b-hydroxylase
    OrganismiTaxus cuspidata (Japanese yew)
    Taxonomic identifieri99806 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaPinidaeCupressalesTaxaceaeTaxus

    Subcellular locationi

    Topology

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei20 – 4021HelicalSequence analysisAdd
    BLAST
    Transmembranei186 – 20621HelicalSequence analysisAdd
    BLAST
    Transmembranei218 – 23821HelicalSequence analysisAdd
    BLAST

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Microsome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 509509Taxoid 14-beta-hydroxylasePRO_0000418753Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliQ84KI1.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PRINTSiPR00463. EP450I.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q84KI1-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MDVFYPLKST VAKFNECFPA ILFIVLSAVA GIVLPLLLFL RSKRRSSVGL
    60 70 80 90 100
    PPGKLGYPFI GESLLFLKAL RSNTVEQFLD ERVKNFGNVF KTSLIGHPTV
    110 120 130 140 150
    VLCGPAGNRL ILANEEKLVQ MSWPKSSMKL MGEKSITAKR GEGHMIIRSA
    160 170 180 190 200
    LQGFFSPGAL QKYIGQMSKT IENHINEKWK GNDQVSVVAL VGDLVFDISA
    210 220 230 240 250
    CLFFNINEKH ERERLFELLE IIAVGVLAVP VDLPGFAYHR ALQARSKLNA
    260 270 280 290 300
    ILSGLIEKRK MDLSSGLATS NQDLLSVFLT FKDDRGNPCS DEEILDNFSG
    310 320 330 340 350
    LLHGSYDTTV SAMACVFKLL SSNPECYEKV VQEQLGILSN KLEGDEITWK
    360 370 380 390 400
    DVKSMKYTWQ VVQETLRLYP SIFGSFRQAI TDIHYNGYII PKGWKLLWTP
    410 420 430 440 450
    YTTHPKEMYF SEPEKFLPSR FDQEGKLVAP YTFLPFGGGQ RSCPGWEFSK
    460 470 480 490 500
    MEILLSVHHF VKTFSTFTPV DPAEIIARDS LCPLPSNGFS VKLFPRSYSL

    HTGNQVKKI
    Length:509
    Mass (Da):57,147
    Last modified:June 1, 2003 - v1
    Checksum:iDE7B3D2980004F15
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AY188177 mRNA. Translation: AAO66199.1.

    Genome annotation databases

    KEGGiag:AAO66199.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AY188177 mRNA. Translation: AAO66199.1.

    3D structure databases

    ProteinModelPortaliQ84KI1.
    ModBaseiSearch...
    MobiDBiSearch...

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    KEGGiag:AAO66199.

    Enzyme and pathway databases

    UniPathwayiUPA00842.
    BioCyciMetaCyc:MONOMER-13409.
    BRENDAi1.14.13.146. 6225.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PRINTSiPR00463. EP450I.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Publicationsi

    1. "Taxoid metabolism: Taxoid 14beta-hydroxylase is a cytochrome P450-dependent monooxygenase."
      Jennewein S., Rithner C.D., Williams R.M., Croteau R.
      Arch. Biochem. Biophys. 413:262-270(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiT14H_TAXCU
    AccessioniPrimary (citable) accession number: Q84KI1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 5, 2012
    Last sequence update: June 1, 2003
    Last modified: May 11, 2016
    This is version 63 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.