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Protein

Taxoid 14-beta-hydroxylase

Gene
N/A
Organism
Taxus cuspidata (Japanese yew)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of 5-alpha-acetoxy-10beta-ol to 5-alpha-acetoxy-10beta,14beta-dihydroxy taxadiene. Also acts on taxa-4(20),11-dien-5-alpha-yl acetate.1 Publication

Catalytic activityi

10-beta-hydroxytaxa-4(20),11-dien-5-alpha-yl acetate + O2 + NADPH = 10-beta,14-beta-dihydroxytaxa-4(20),11-dien-5?-yl acetate + NADP+ + H2O.1 Publication

Kineticsi

  1. KM=33 µM for 5-alpha-acetoxy taxadiene1 Publication
  2. KM=55 µM for 5-alpha-acetoxy-10-beta-hydroxy taxadiene1 Publication

    pH dependencei

    Optimum pH is 7.5.1 Publication

    Pathwayi: taxol biosynthesis

    This protein is involved in the pathway taxol biosynthesis, which is part of Alkaloid biosynthesis.
    View all proteins of this organism that are known to be involved in the pathway taxol biosynthesis and in Alkaloid biosynthesis.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Metal bindingi443Iron (heme axial ligand)By similarity1

    GO - Molecular functioni

    • heme binding Source: InterPro
    • iron ion binding Source: InterPro
    • taxoid 14-beta-hydroxylase activity Source: UniProtKB

    GO - Biological processi

    • oxidation-reduction process Source: UniProtKB
    • paclitaxel biosynthetic process Source: UniProtKB-UniPathway
    • paclitaxel metabolic process Source: UniProtKB

    Keywordsi

    Molecular functionMonooxygenase, Oxidoreductase
    Biological processTaxol biosynthesis
    LigandHeme, Iron, Metal-binding, NADP

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-13409
    BRENDAi1.14.13.146 6225
    UniPathwayiUPA00842

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Taxoid 14-beta-hydroxylase (EC:1.14.13.146)
    Alternative name(s):
    Taxane 14b-hydroxylase
    OrganismiTaxus cuspidata (Japanese yew)
    Taxonomic identifieri99806 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaPinidaeCupressalesTaxaceaeTaxus

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Transmembranei20 – 40HelicalSequence analysisAdd BLAST21
    Transmembranei186 – 206HelicalSequence analysisAdd BLAST21
    Transmembranei218 – 238HelicalSequence analysisAdd BLAST21

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Microsome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00004187531 – 509Taxoid 14-beta-hydroxylaseAdd BLAST509

    Proteomic databases

    PRIDEiQ84KI1

    Structurei

    3D structure databases

    ProteinModelPortaliQ84KI1
    SMRiQ84KI1
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    KOiK20512

    Family and domain databases

    Gene3Di1.10.630.10, 1 hit
    InterProiView protein in InterPro
    IPR001128 Cyt_P450
    IPR017972 Cyt_P450_CS
    IPR002401 Cyt_P450_E_grp-I
    IPR036396 Cyt_P450_sf
    PfamiView protein in Pfam
    PF00067 p450, 1 hit
    PRINTSiPR00463 EP450I
    PR00385 P450
    SUPFAMiSSF48264 SSF48264, 1 hit
    PROSITEiView protein in PROSITE
    PS00086 CYTOCHROME_P450, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Q84KI1-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MDVFYPLKST VAKFNECFPA ILFIVLSAVA GIVLPLLLFL RSKRRSSVGL
    60 70 80 90 100
    PPGKLGYPFI GESLLFLKAL RSNTVEQFLD ERVKNFGNVF KTSLIGHPTV
    110 120 130 140 150
    VLCGPAGNRL ILANEEKLVQ MSWPKSSMKL MGEKSITAKR GEGHMIIRSA
    160 170 180 190 200
    LQGFFSPGAL QKYIGQMSKT IENHINEKWK GNDQVSVVAL VGDLVFDISA
    210 220 230 240 250
    CLFFNINEKH ERERLFELLE IIAVGVLAVP VDLPGFAYHR ALQARSKLNA
    260 270 280 290 300
    ILSGLIEKRK MDLSSGLATS NQDLLSVFLT FKDDRGNPCS DEEILDNFSG
    310 320 330 340 350
    LLHGSYDTTV SAMACVFKLL SSNPECYEKV VQEQLGILSN KLEGDEITWK
    360 370 380 390 400
    DVKSMKYTWQ VVQETLRLYP SIFGSFRQAI TDIHYNGYII PKGWKLLWTP
    410 420 430 440 450
    YTTHPKEMYF SEPEKFLPSR FDQEGKLVAP YTFLPFGGGQ RSCPGWEFSK
    460 470 480 490 500
    MEILLSVHHF VKTFSTFTPV DPAEIIARDS LCPLPSNGFS VKLFPRSYSL

    HTGNQVKKI
    Length:509
    Mass (Da):57,147
    Last modified:June 1, 2003 - v1
    Checksum:iDE7B3D2980004F15
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AY188177 mRNA Translation: AAO66199.1

    Genome annotation databases

    KEGGiag:AAO66199

    Similar proteinsi

    Entry informationi

    Entry nameiT14H_TAXCU
    AccessioniPrimary (citable) accession number: Q84KI1
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 5, 2012
    Last sequence update: June 1, 2003
    Last modified: May 23, 2018
    This is version 69 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
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