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Q84K11 (PPP5_SOLLC) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serine/threonine-protein phosphatase 5

EC=3.1.3.16
Alternative name(s):
LePP5
Gene names
Name:PP5
OrganismSolanum lycopersicum (Tomato) (Lycopersicon esculentum) [Reference proteome]
Taxonomic identifier4081 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeSolaneaeSolanumLycopersicon

Protein attributes

Sequence length556 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Isoform 2 dephosphorylates phosphorylated phytochromes, with a preference toward Pfr forms, and enhances phytochrome-mediated photoresponses By similarity. Can use para-nitrophenylphosphate (pNPP) and phosphorylated casein as substrate at pH 7.5 and 5.0. Ref.1

Catalytic activity

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactor

Binds 2 manganese ions per subunit By similarity.

Enzyme regulation

Activated by linoleic acid, linolenic acid, oleic acid, and arachidonic acid (AA).

Subunit structure

Interacts with PHYA and PHYB, mostly when they are phosphorylated and in Pfr forms By similarity.

Subcellular location

Isoform 1: Endoplasmic reticulum membrane; Multi-pass membrane protein. Nucleus membrane; Multi-pass membrane protein Ref.1.

Isoform 2: Cytoplasm. Nucleusnucleoplasm. Nucleus speckle. Note: Cytoplasmic in darkness, but translocated to the nucleus upon illumination, when associated with phytochromes into speckles By similarity. Ref.1

Tissue specificity

Expressed in roots, stems, leaves, flowers, and fruits. Ref.1

Domain

TPR repeats are required for the binding with phytochromes By similarity.

Sequence similarities

Belongs to the PPP phosphatase family. PP-5 (PP-T) subfamily.

Contains 3 TPR repeats.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q84K11-1)

Also known as: 62 kDa isoform;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q84K11-2)

Also known as: 55 kDa isoform;

The sequence of this isoform differs from the canonical sequence as follows:
     158-228: Missing.
Note: Partial isoform 2 (148-485) lacking TPR repeats exhibits enhanced activity at pH 7.5 but not at pH 5.0 with phosphocasein as substrate. This partial protein is in addition inhibited by okadaic acid.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 556556Serine/threonine-protein phosphatase 5
PRO_0000308995

Regions

Transmembrane171 – 19121Helical; Potential
Transmembrane192 – 21221Helical; Potential
Repeat14 – 4734TPR 1
Repeat49 – 8133TPR 2
Repeat82 – 11534TPR 3

Sites

Active site3621Proton donor By similarity
Metal binding3001Manganese 1 By similarity
Metal binding3021Manganese 1 By similarity
Metal binding3291Manganese 1 By similarity
Metal binding3291Manganese 2 By similarity
Metal binding3611Manganese 2 By similarity
Metal binding4101Manganese 2 By similarity
Metal binding4851Manganese 2 By similarity

Natural variations

Alternative sequence158 – 22871Missing in isoform 2.
VSP_029090

Experimental info

Mutagenesis4111G → R: Loss of activity; in isoform 2. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (62 kDa isoform) [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 39262299C9B27866

FASTA55661,860
        10         20         30         40         50         60 
MPGMEAENSN ASRAEELKQL ANEAFKGHKY SQAIDLYTQA IELNGENAVY YANRAFAHTK 

        70         80         90        100        110        120 
LEEYGSAIQD GTRAIEIDPR YSKGYYRRGA AYLAMGKFKD ALKDFQQVKK LCPNDPDATK 

       130        140        150        160        170        180 
KLKECEKAVM KLKFEEAISV PESQRRSVAD SIDYRSVGSG PGSSYVPTKT TAVSAAAALM 

       190        200        210        220        230        240 
GVLVVYMGTK AATMVAAAAS AALLVVLITF LWGRCSDGFF TKSRTLELEV EPQYAGARIE 

       250        260        270        280        290        300 
GDVVTLDFVK KMLDDFKNQK NLHKRYAYQI VLQTREMLRA LPSLVDIVVP EGKHFTVCGD 

       310        320        330        340        350        360 
VHGQFYDLLN IFELNGLPSE DNPYLFNGDF VDRGSFSLEV ILTLFAFKCM CPSAIHLARG 

       370        380        390        400        410        420 
NHESKSMNKI YGFEGEVRSK LSEIFVELFA EVFCCLPLAH VINEKVFVVH GGLFSVDGVK 

       430        440        450        460        470        480 
LSDIRAIDRF CEPPEEGLMC ELLWSDPQPQ PGRGPSKRGV GLSFGGDVTK RFLQENNLDL 

       490        500        510        520        530        540 
VVRSHEVKDE GYEIEHDGKL ITVFSAPNYC DQMGNKGAFI RFEAPDMKPN IVTFSAVPHP 

       550 
DVKPMAYANN FLRMFS 

« Hide

Isoform 2 (55 kDa isoform) [UniParc].

Checksum: FCF68708D5520E68
Show »

FASTA48554,720

References

[1]"The subcellular localization of plant protein phosphatase 5 isoforms is determined by alternative splicing."
de la Fuente van Bentem S., Vossen J.H., Vermeer J.E.M., de Vroomen M.J., Gadella T.W.J. Jr., Haring M.A., Cornelissen B.J.C.
Plant Physiol. 133:702-712(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 1 AND 2), MUTAGENESIS OF GLY-411, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, ALTERNATIVE PRODUCTS, ACTIVATION BY FATTY ACIDS.
Strain: cv. GCR161 and cv. VFN8.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY150041 mRNA. Translation: AAN64317.1.
AY182777 mRNA. Translation: AAO26213.1.
AY182778 Genomic DNA. Translation: AAO26214.1.
AY182778 Genomic DNA. Translation: AAO26215.1.
RefSeqNP_001234232.1. NM_001247303.1. [Q84K11-2]
UniGeneLes.3965.

3D structure databases

ProteinModelPortalQ84K11.
SMRQ84K11. Positions 9-159, 233-554.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID543849.
KEGGsly:543849.

Phylogenomic databases

KOK04460.

Family and domain databases

Gene3D1.25.40.10. 1 hit.
3.60.21.10. 1 hit.
InterProIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR013235. PPP_dom.
IPR006186. Ser/Thr-sp_prot-phosphatase.
IPR011236. Ser/Thr_PPase_5.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical.
IPR001440. TPR_1.
IPR019734. TPR_repeat.
[Graphical view]
PANTHERPTHR11668:SF12. PTHR11668:SF12. 1 hit.
PfamPF00149. Metallophos. 1 hit.
PF08321. PPP5. 2 hits.
PF00515. TPR_1. 1 hit.
[Graphical view]
PIRSFPIRSF033096. PPPtase_5. 1 hit.
PRINTSPR00114. STPHPHTASE.
SMARTSM00156. PP2Ac. 1 hit.
SM00028. TPR. 3 hits.
[Graphical view]
SUPFAMSSF56300. SSF56300. 1 hit.
PROSITEPS50005. TPR. 3 hits.
PS50293. TPR_REGION. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePPP5_SOLLC
AccessionPrimary (citable) accession number: Q84K11
Secondary accession number(s): Q8H1H4
Entry history
Integrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: June 1, 2003
Last modified: June 11, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families