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Q84K11

- PPP5_SOLLC

UniProt

Q84K11 - PPP5_SOLLC

Protein

Serine/threonine-protein phosphatase 5

Gene

PP5

Organism
Solanum lycopersicum (Tomato) (Lycopersicon esculentum)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 75 (01 Oct 2014)
      Sequence version 1 (01 Jun 2003)
      Previous versions | rss
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    Functioni

    Isoform 2 dephosphorylates phosphorylated phytochromes, with a preference toward Pfr forms, and enhances phytochrome-mediated photoresponses By similarity. Can use para-nitrophenylphosphate (pNPP) and phosphorylated casein as substrate at pH 7.5 and 5.0.By similarity1 Publication

    Catalytic activityi

    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

    Cofactori

    Binds 2 manganese ions per subunit.By similarity

    Enzyme regulationi

    Activated by linoleic acid, linolenic acid, oleic acid, and arachidonic acid (AA).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi300 – 3001Manganese 1By similarity
    Metal bindingi302 – 3021Manganese 1By similarity
    Metal bindingi329 – 3291Manganese 1By similarity
    Metal bindingi329 – 3291Manganese 2By similarity
    Metal bindingi361 – 3611Manganese 2By similarity
    Active sitei362 – 3621Proton donorBy similarity
    Metal bindingi410 – 4101Manganese 2By similarity
    Metal bindingi485 – 4851Manganese 2By similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. phosphoprotein phosphatase activity Source: UniProtKB-KW

    GO - Biological processi

    1. protein dephosphorylation Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Ligandi

    Manganese, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein phosphatase 5 (EC:3.1.3.16)
    Alternative name(s):
    LePP5
    Gene namesi
    Name:PP5
    OrganismiSolanum lycopersicum (Tomato) (Lycopersicon esculentum)
    Taxonomic identifieri4081 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeSolaneaeSolanumLycopersicon
    ProteomesiUP000004994: Unplaced

    Subcellular locationi

    Isoform 2 : Cytoplasm. Nucleusnucleoplasm. Nucleus speckle
    Note: Cytoplasmic in darkness, but translocated to the nucleus upon illumination, when associated with phytochromes into speckles.By similarity

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW
    3. nuclear membrane Source: UniProtKB-SubCell
    4. nuclear speck Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Endoplasmic reticulum, Membrane, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi411 – 4111G → R: Loss of activity; in isoform 2. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 556556Serine/threonine-protein phosphatase 5PRO_0000308995Add
    BLAST

    Expressioni

    Tissue specificityi

    Expressed in roots, stems, leaves, flowers, and fruits.1 Publication

    Interactioni

    Subunit structurei

    Interacts with PHYA and PHYB, mostly when they are phosphorylated and in Pfr forms.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ84K11.
    SMRiQ84K11. Positions 9-159, 233-554.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei171 – 19121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei192 – 21221HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati14 – 4734TPR 1Add
    BLAST
    Repeati49 – 8133TPR 2Add
    BLAST
    Repeati82 – 11534TPR 3Add
    BLAST

    Domaini

    TPR repeats are required for the binding with phytochromes.By similarity

    Sequence similaritiesi

    Contains 3 TPR repeats.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, TPR repeat, Transmembrane, Transmembrane helix

    Phylogenomic databases

    KOiK04460.

    Family and domain databases

    Gene3Di1.25.40.10. 1 hit.
    3.60.21.10. 1 hit.
    InterProiIPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR000909. PLipase_C_PInositol-sp_X_dom.
    IPR013235. PPP_dom.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    IPR011236. Ser/Thr_PPase_5.
    IPR013026. TPR-contain_dom.
    IPR011990. TPR-like_helical.
    IPR001440. TPR_1.
    IPR019734. TPR_repeat.
    [Graphical view]
    PANTHERiPTHR11668:SF12. PTHR11668:SF12. 1 hit.
    PfamiPF00149. Metallophos. 1 hit.
    PF08321. PPP5. 2 hits.
    PF00515. TPR_1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF033096. PPPtase_5. 1 hit.
    PRINTSiPR00114. STPHPHTASE.
    SMARTiSM00156. PP2Ac. 1 hit.
    SM00028. TPR. 3 hits.
    [Graphical view]
    SUPFAMiSSF56300. SSF56300. 1 hit.
    PROSITEiPS50005. TPR. 3 hits.
    PS50293. TPR_REGION. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q84K11-1) [UniParc]FASTAAdd to Basket

    Also known as: 62 kDa isoform

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MPGMEAENSN ASRAEELKQL ANEAFKGHKY SQAIDLYTQA IELNGENAVY    50
    YANRAFAHTK LEEYGSAIQD GTRAIEIDPR YSKGYYRRGA AYLAMGKFKD 100
    ALKDFQQVKK LCPNDPDATK KLKECEKAVM KLKFEEAISV PESQRRSVAD 150
    SIDYRSVGSG PGSSYVPTKT TAVSAAAALM GVLVVYMGTK AATMVAAAAS 200
    AALLVVLITF LWGRCSDGFF TKSRTLELEV EPQYAGARIE GDVVTLDFVK 250
    KMLDDFKNQK NLHKRYAYQI VLQTREMLRA LPSLVDIVVP EGKHFTVCGD 300
    VHGQFYDLLN IFELNGLPSE DNPYLFNGDF VDRGSFSLEV ILTLFAFKCM 350
    CPSAIHLARG NHESKSMNKI YGFEGEVRSK LSEIFVELFA EVFCCLPLAH 400
    VINEKVFVVH GGLFSVDGVK LSDIRAIDRF CEPPEEGLMC ELLWSDPQPQ 450
    PGRGPSKRGV GLSFGGDVTK RFLQENNLDL VVRSHEVKDE GYEIEHDGKL 500
    ITVFSAPNYC DQMGNKGAFI RFEAPDMKPN IVTFSAVPHP DVKPMAYANN 550
    FLRMFS 556
    Length:556
    Mass (Da):61,860
    Last modified:June 1, 2003 - v1
    Checksum:i39262299C9B27866
    GO
    Isoform 2 (identifier: Q84K11-2) [UniParc]FASTAAdd to Basket

    Also known as: 55 kDa isoform

    The sequence of this isoform differs from the canonical sequence as follows:
         158-228: Missing.

    Note: Partial isoform 2 (148-485) lacking TPR repeats exhibits enhanced activity at pH 7.5 but not at pH 5.0 with phosphocasein as substrate. This partial protein is in addition inhibited by okadaic acid.

    Show »
    Length:485
    Mass (Da):54,720
    Checksum:iFCF68708D5520E68
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei158 – 22871Missing in isoform 2. 1 PublicationVSP_029090Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY150041 mRNA. Translation: AAN64317.1.
    AY182777 mRNA. Translation: AAO26213.1.
    AY182778 Genomic DNA. Translation: AAO26214.1.
    AY182778 Genomic DNA. Translation: AAO26215.1.
    RefSeqiNP_001234232.1. NM_001247303.1. [Q84K11-2]
    UniGeneiLes.3965.

    Genome annotation databases

    GeneIDi543849.
    KEGGisly:543849.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY150041 mRNA. Translation: AAN64317.1 .
    AY182777 mRNA. Translation: AAO26213.1 .
    AY182778 Genomic DNA. Translation: AAO26214.1 .
    AY182778 Genomic DNA. Translation: AAO26215.1 .
    RefSeqi NP_001234232.1. NM_001247303.1. [Q84K11-2 ]
    UniGenei Les.3965.

    3D structure databases

    ProteinModelPortali Q84K11.
    SMRi Q84K11. Positions 9-159, 233-554.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 543849.
    KEGGi sly:543849.

    Phylogenomic databases

    KOi K04460.

    Family and domain databases

    Gene3Di 1.25.40.10. 1 hit.
    3.60.21.10. 1 hit.
    InterProi IPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR000909. PLipase_C_PInositol-sp_X_dom.
    IPR013235. PPP_dom.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    IPR011236. Ser/Thr_PPase_5.
    IPR013026. TPR-contain_dom.
    IPR011990. TPR-like_helical.
    IPR001440. TPR_1.
    IPR019734. TPR_repeat.
    [Graphical view ]
    PANTHERi PTHR11668:SF12. PTHR11668:SF12. 1 hit.
    Pfami PF00149. Metallophos. 1 hit.
    PF08321. PPP5. 2 hits.
    PF00515. TPR_1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF033096. PPPtase_5. 1 hit.
    PRINTSi PR00114. STPHPHTASE.
    SMARTi SM00156. PP2Ac. 1 hit.
    SM00028. TPR. 3 hits.
    [Graphical view ]
    SUPFAMi SSF56300. SSF56300. 1 hit.
    PROSITEi PS50005. TPR. 3 hits.
    PS50293. TPR_REGION. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The subcellular localization of plant protein phosphatase 5 isoforms is determined by alternative splicing."
      de la Fuente van Bentem S., Vossen J.H., Vermeer J.E.M., de Vroomen M.J., Gadella T.W.J. Jr., Haring M.A., Cornelissen B.J.C.
      Plant Physiol. 133:702-712(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 1 AND 2), MUTAGENESIS OF GLY-411, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, ALTERNATIVE PRODUCTS, ACTIVATION BY FATTY ACIDS.
      Strain: cv. GCR161 and cv. VFN8.

    Entry informationi

    Entry nameiPPP5_SOLLC
    AccessioniPrimary (citable) accession number: Q84K11
    Secondary accession number(s): Q8H1H4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 13, 2007
    Last sequence update: June 1, 2003
    Last modified: October 1, 2014
    This is version 75 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3