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Protein

Serine/threonine-protein phosphatase 5

Gene

PP5

Organism
Solanum lycopersicum (Tomato) (Lycopersicon esculentum)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Isoform 2 dephosphorylates phosphorylated phytochromes, with a preference toward Pfr forms, and enhances phytochrome-mediated photoresponses (By similarity). Can use para-nitrophenylphosphate (pNPP) and phosphorylated casein as substrate at pH 7.5 and 5.0.By similarity1 Publication

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity

Enzyme regulationi

Activated by linoleic acid, linolenic acid, oleic acid, and arachidonic acid (AA).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi300 – 3001Manganese 1By similarity
Metal bindingi302 – 3021Manganese 1By similarity
Metal bindingi329 – 3291Manganese 1By similarity
Metal bindingi329 – 3291Manganese 2By similarity
Metal bindingi361 – 3611Manganese 2By similarity
Active sitei362 – 3621Proton donorBy similarity
Metal bindingi410 – 4101Manganese 2By similarity
Metal bindingi485 – 4851Manganese 2By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. phosphoprotein phosphatase activity Source: UniProtKB-KW

GO - Biological processi

  1. protein dephosphorylation Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Manganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase 5 (EC:3.1.3.16)
Alternative name(s):
LePP5
Gene namesi
Name:PP5
OrganismiSolanum lycopersicum (Tomato) (Lycopersicon esculentum)
Taxonomic identifieri4081 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeSolaneaeSolanumLycopersicon
ProteomesiUP000004994: Unplaced

Subcellular locationi

Isoform 2 : Cytoplasm. Nucleusnucleoplasm. Nucleus speckle
Note: Cytoplasmic in darkness, but translocated to the nucleus upon illumination, when associated with phytochromes into speckles.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei171 – 19121HelicalSequence AnalysisAdd
BLAST
Transmembranei192 – 21221HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
  3. nuclear membrane Source: UniProtKB-SubCell
  4. nuclear speck Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Membrane, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi411 – 4111G → R: Loss of activity; in isoform 2. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 556556Serine/threonine-protein phosphatase 5PRO_0000308995Add
BLAST

Expressioni

Tissue specificityi

Expressed in roots, stems, leaves, flowers, and fruits.1 Publication

Interactioni

Subunit structurei

Interacts with PHYA and PHYB, mostly when they are phosphorylated and in Pfr forms.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ84K11.
SMRiQ84K11. Positions 9-159, 233-554.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati14 – 4734TPR 1Add
BLAST
Repeati49 – 8133TPR 2Add
BLAST
Repeati82 – 11534TPR 3Add
BLAST

Domaini

TPR repeats are required for the binding with phytochromes.By similarity

Sequence similaritiesi

Contains 3 TPR repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, TPR repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

InParanoidiQ84K11.
KOiK04460.

Family and domain databases

Gene3Di1.25.40.10. 1 hit.
3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR013235. PPP_dom.
IPR006186. Ser/Thr-sp_prot-phosphatase.
IPR011236. Ser/Thr_PPase_5.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR001440. TPR_1.
IPR019734. TPR_repeat.
[Graphical view]
PANTHERiPTHR11668:SF12. PTHR11668:SF12. 1 hit.
PfamiPF00149. Metallophos. 1 hit.
PF08321. PPP5. 2 hits.
PF00515. TPR_1. 1 hit.
[Graphical view]
PIRSFiPIRSF033096. PPPtase_5. 1 hit.
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
SM00028. TPR. 3 hits.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS50005. TPR. 3 hits.
PS50293. TPR_REGION. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q84K11-1) [UniParc]FASTAAdd to Basket

Also known as: 62 kDa isoform

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPGMEAENSN ASRAEELKQL ANEAFKGHKY SQAIDLYTQA IELNGENAVY
60 70 80 90 100
YANRAFAHTK LEEYGSAIQD GTRAIEIDPR YSKGYYRRGA AYLAMGKFKD
110 120 130 140 150
ALKDFQQVKK LCPNDPDATK KLKECEKAVM KLKFEEAISV PESQRRSVAD
160 170 180 190 200
SIDYRSVGSG PGSSYVPTKT TAVSAAAALM GVLVVYMGTK AATMVAAAAS
210 220 230 240 250
AALLVVLITF LWGRCSDGFF TKSRTLELEV EPQYAGARIE GDVVTLDFVK
260 270 280 290 300
KMLDDFKNQK NLHKRYAYQI VLQTREMLRA LPSLVDIVVP EGKHFTVCGD
310 320 330 340 350
VHGQFYDLLN IFELNGLPSE DNPYLFNGDF VDRGSFSLEV ILTLFAFKCM
360 370 380 390 400
CPSAIHLARG NHESKSMNKI YGFEGEVRSK LSEIFVELFA EVFCCLPLAH
410 420 430 440 450
VINEKVFVVH GGLFSVDGVK LSDIRAIDRF CEPPEEGLMC ELLWSDPQPQ
460 470 480 490 500
PGRGPSKRGV GLSFGGDVTK RFLQENNLDL VVRSHEVKDE GYEIEHDGKL
510 520 530 540 550
ITVFSAPNYC DQMGNKGAFI RFEAPDMKPN IVTFSAVPHP DVKPMAYANN

FLRMFS
Length:556
Mass (Da):61,860
Last modified:June 1, 2003 - v1
Checksum:i39262299C9B27866
GO
Isoform 2 (identifier: Q84K11-2) [UniParc]FASTAAdd to Basket

Also known as: 55 kDa isoform

The sequence of this isoform differs from the canonical sequence as follows:
     158-228: Missing.

Note: Partial isoform 2 (148-485) lacking TPR repeats exhibits enhanced activity at pH 7.5 but not at pH 5.0 with phosphocasein as substrate. This partial protein is in addition inhibited by okadaic acid.

Show »
Length:485
Mass (Da):54,720
Checksum:iFCF68708D5520E68
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei158 – 22871Missing in isoform 2. 1 PublicationVSP_029090Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY150041 mRNA. Translation: AAN64317.1.
AY182777 mRNA. Translation: AAO26213.1.
AY182778 Genomic DNA. Translation: AAO26214.1.
AY182778 Genomic DNA. Translation: AAO26215.1.
RefSeqiNP_001234232.1. NM_001247303.1. [Q84K11-2]
UniGeneiLes.3965.

Genome annotation databases

GeneIDi543849.
KEGGisly:543849.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY150041 mRNA. Translation: AAN64317.1.
AY182777 mRNA. Translation: AAO26213.1.
AY182778 Genomic DNA. Translation: AAO26214.1.
AY182778 Genomic DNA. Translation: AAO26215.1.
RefSeqiNP_001234232.1. NM_001247303.1. [Q84K11-2]
UniGeneiLes.3965.

3D structure databases

ProteinModelPortaliQ84K11.
SMRiQ84K11. Positions 9-159, 233-554.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi543849.
KEGGisly:543849.

Phylogenomic databases

InParanoidiQ84K11.
KOiK04460.

Family and domain databases

Gene3Di1.25.40.10. 1 hit.
3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR013235. PPP_dom.
IPR006186. Ser/Thr-sp_prot-phosphatase.
IPR011236. Ser/Thr_PPase_5.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR001440. TPR_1.
IPR019734. TPR_repeat.
[Graphical view]
PANTHERiPTHR11668:SF12. PTHR11668:SF12. 1 hit.
PfamiPF00149. Metallophos. 1 hit.
PF08321. PPP5. 2 hits.
PF00515. TPR_1. 1 hit.
[Graphical view]
PIRSFiPIRSF033096. PPPtase_5. 1 hit.
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
SM00028. TPR. 3 hits.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS50005. TPR. 3 hits.
PS50293. TPR_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The subcellular localization of plant protein phosphatase 5 isoforms is determined by alternative splicing."
    de la Fuente van Bentem S., Vossen J.H., Vermeer J.E.M., de Vroomen M.J., Gadella T.W.J. Jr., Haring M.A., Cornelissen B.J.C.
    Plant Physiol. 133:702-712(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 1 AND 2), MUTAGENESIS OF GLY-411, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, ALTERNATIVE PRODUCTS, ACTIVATION BY FATTY ACIDS.
    Strain: cv. GCR161 and cv. VFN8.

Entry informationi

Entry nameiPPP5_SOLLC
AccessioniPrimary (citable) accession number: Q84K11
Secondary accession number(s): Q8H1H4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: June 1, 2003
Last modified: January 7, 2015
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.