Q84K11 (PPP5_SOLLC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein phosphatase 5
EC=3.1.3.16

Alternative name(s):
LePP5

Gene names

Name:

PP5

Organism

Solanum lycopersicum (Tomato) (Lycopersicon esculentum) [Reference proteome]

Taxonomic identifier

4081 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Solaneae › Solanum › Lycopersicon

Protein attributes

Sequence length	556 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Isoform 2 dephosphorylates phosphorylated phytochromes, with a preference toward Pfr forms, and enhances phytochrome-mediated photoresponses By similarity. Can use para-nitrophenylphosphate (pNPP) and phosphorylated casein as substrate at pH 7.5 and 5.0. Ref.1
Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Binds 1 iron ion per subunit By similarity. Binds 1 manganese ion per subunit By similarity.
Enzyme regulation	Activated by linoleic acid, linolenic acid, oleic acid, and arachidonic acid (AA).
Subunit structure	Interacts with PHYA and PHYB, mostly when they are phosphorylated and in Pfr forms By similarity.
Subcellular location	Isoform 1: Endoplasmic reticulum membrane; Multi-pass membrane protein. Nucleus membrane; Multi-pass membrane protein Ref.1. Isoform 2: Cytoplasm. Nucleus › nucleoplasm. Nucleus speckle. Note: Cytoplasmic in darkness, but translocated to the nucleus upon illumination, when associated with phytochromes into speckles By similarity. Ref.1
Tissue specificity	Expressed in roots, stems, leaves, flowers, and fruits. Ref.1
Domain	TPR repeats are required for the binding with phytochromes By similarity.
Sequence similarities	Belongs to the PPP phosphatase family. PP-5 (PP-T) subfamily. Contains 3 TPR repeats.

Ontologies

Keywords
Cellular component	Cytoplasm Endoplasmic reticulum Membrane Nucleus
Coding sequence diversity	Alternative splicing
Domain	Repeat TPR repeat Transmembrane Transmembrane helix
Ligand	Iron Manganese Metal-binding
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein dephosphorylation Inferred from electronic annotation. Source: InterPro
Cellular_component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW nuclear membrane Inferred from electronic annotation. Source: UniProtKB-SubCell nuclear speck Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q84K11-1)

Also known as: 62 kDa isoform;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q84K11-2)

Also known as: 55 kDa isoform;

The sequence of this isoform differs from the canonical sequence as follows:
     158-228: Missing.

Note: Partial isoform 2 (148-485) lacking TPR repeats exhibits enhanced activity at pH 7.5 but not at pH 5.0 with phosphocasein as substrate. This partial protein is in addition inhibited by okadaic acid.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 556	556	Serine/threonine-protein phosphatase 5		PRO_0000308995
Regions
	Transmembrane	171 – 191	21	Helical; Potential
	Transmembrane	192 – 212	21	Helical; Potential
	Repeat	14 – 47	34	TPR 1
	Repeat	49 – 81	33	TPR 2
	Repeat	82 – 115	34	TPR 3
Sites
	Active site	362	1	Proton donor By similarity
	Metal binding	300	1	Iron By similarity
	Metal binding	302	1	Iron By similarity
	Metal binding	329	1	Iron By similarity
	Metal binding	329	1	Manganese By similarity
	Metal binding	361	1	Manganese By similarity
	Metal binding	410	1	Manganese By similarity
	Metal binding	485	1	Manganese By similarity
Natural variations
	Alternative sequence	158 – 228	71	Missing in isoform 2.		VSP_029090
Experimental info
	Mutagenesis	411	1	G → R: Loss of activity; in isoform 2. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (62 kDa isoform) [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 39262299C9B27866	FASTA	556	61,860
	10 20 30 40 50 60 MPGMEAENSN ASRAEELKQL ANEAFKGHKY SQAIDLYTQA IELNGENAVY YANRAFAHTK 70 80 90 100 110 120 LEEYGSAIQD GTRAIEIDPR YSKGYYRRGA AYLAMGKFKD ALKDFQQVKK LCPNDPDATK 130 140 150 160 170 180 KLKECEKAVM KLKFEEAISV PESQRRSVAD SIDYRSVGSG PGSSYVPTKT TAVSAAAALM 190 200 210 220 230 240 GVLVVYMGTK AATMVAAAAS AALLVVLITF LWGRCSDGFF TKSRTLELEV EPQYAGARIE 250 260 270 280 290 300 GDVVTLDFVK KMLDDFKNQK NLHKRYAYQI VLQTREMLRA LPSLVDIVVP EGKHFTVCGD 310 320 330 340 350 360 VHGQFYDLLN IFELNGLPSE DNPYLFNGDF VDRGSFSLEV ILTLFAFKCM CPSAIHLARG 370 380 390 400 410 420 NHESKSMNKI YGFEGEVRSK LSEIFVELFA EVFCCLPLAH VINEKVFVVH GGLFSVDGVK 430 440 450 460 470 480 LSDIRAIDRF CEPPEEGLMC ELLWSDPQPQ PGRGPSKRGV GLSFGGDVTK RFLQENNLDL 490 500 510 520 530 540 VVRSHEVKDE GYEIEHDGKL ITVFSAPNYC DQMGNKGAFI RFEAPDMKPN IVTFSAVPHP 550 DVKPMAYANN FLRMFS
	Isoform 2 (55 kDa isoform) [UniParc]. Checksum: FCF68708D5520E68	FASTA	485	54,720
	10 20 30 40 50 60 MPGMEAENSN ASRAEELKQL ANEAFKGHKY SQAIDLYTQA IELNGENAVY YANRAFAHTK 70 80 90 100 110 120 LEEYGSAIQD GTRAIEIDPR YSKGYYRRGA AYLAMGKFKD ALKDFQQVKK LCPNDPDATK 130 140 150 160 170 180 KLKECEKAVM KLKFEEAISV PESQRRSVAD SIDYRSVEVE PQYAGARIEG DVVTLDFVKK 190 200 210 220 230 240 MLDDFKNQKN LHKRYAYQIV LQTREMLRAL PSLVDIVVPE GKHFTVCGDV HGQFYDLLNI 250 260 270 280 290 300 FELNGLPSED NPYLFNGDFV DRGSFSLEVI LTLFAFKCMC PSAIHLARGN HESKSMNKIY 310 320 330 340 350 360 GFEGEVRSKL SEIFVELFAE VFCCLPLAHV INEKVFVVHG GLFSVDGVKL SDIRAIDRFC 370 380 390 400 410 420 EPPEEGLMCE LLWSDPQPQP GRGPSKRGVG LSFGGDVTKR FLQENNLDLV VRSHEVKDEG 430 440 450 460 470 480 YEIEHDGKLI TVFSAPNYCD QMGNKGAFIR FEAPDMKPNI VTFSAVPHPD VKPMAYANNF LRMFS

References

[1]

"The subcellular localization of plant protein phosphatase 5 isoforms is determined by alternative splicing." de la Fuente van Bentem S., Vossen J.H., Vermeer J.E.M., de Vroomen M.J., Gadella T.W.J. Jr., Haring M.A., Cornelissen B.J.C. Plant Physiol. 133:702-712(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 1 AND 2), MUTAGENESIS OF GLY-411, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, ALTERNATIVE PRODUCTS, ACTIVATION BY FATTY ACIDS. Strain: cv. GCR161 and cv. VFN8.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY150041 mRNA. Translation: AAN64317.1. AY182777 mRNA. Translation: AAO26213.1. AY182778 Genomic DNA. Translation: AAO26214.1. AY182778 Genomic DNA. Translation: AAO26215.1.
RefSeq	NP_001234232.1. NM_001247303.1.
UniGene	Les.3965.
3D structure databases
HSSP	HSSP built from PDB template 1S95 based on UniProtKB P53041.
ProteinModelPortal	Q84K11.
SMR	Q84K11. Positions 9-159, 233-554.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	543849.
Family and domain databases
Gene3D	1.25.40.10. 1 hit.
InterPro	IPR004843. Metallo_PEstase_dom. IPR000909. PLipase_C_PInositol-sp_X_dom. IPR013235. PPP_dom. IPR006186. Ser/Thr-sp_prot-phosphatase. IPR011236. Ser/Thr_PPase_5. IPR001440. TPR-1. IPR013026. TPR-contain_dom. IPR011990. TPR-like_helical. IPR019734. TPR_repeat. [Graphical view]
PANTHER	PTHR11668:SF12. PTHR11668:SF12. 1 hit.
Pfam	PF00149. Metallophos. 1 hit. PF08321. PPP5. 2 hits. PF00515. TPR_1. 1 hit. [Graphical view]
PIRSF	PIRSF033096. PPPtase_5. 1 hit.
PRINTS	PR00114. STPHPHTASE.
SMART	SM00156. PP2Ac. 1 hit. SM00028. TPR. 3 hits. [Graphical view]
PROSITE	PS00125. SER_THR_PHOSPHATASE. False negative. PS50005. TPR. 3 hits. PS50293. TPR_REGION. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PPP5_SOLLC

Accession

Primary (citable) accession number: Q84K11
Secondary accession number(s): Q8H1H4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 13, 2007
Last sequence update:	June 1, 2003
Last modified:	April 3, 2013
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program