SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q84K11

- PPP5_SOLLC

UniProt

Q84K11 - PPP5_SOLLC

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Serine/threonine-protein phosphatase 5

Gene
PP5
Organism
Solanum lycopersicum (Tomato) (Lycopersicon esculentum)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Isoform 2 dephosphorylates phosphorylated phytochromes, with a preference toward Pfr forms, and enhances phytochrome-mediated photoresponses By similarity. Can use para-nitrophenylphosphate (pNPP) and phosphorylated casein as substrate at pH 7.5 and 5.0.1 Publication

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Binds 2 manganese ions per subunit By similarity.

Enzyme regulationi

Activated by linoleic acid, linolenic acid, oleic acid, and arachidonic acid (AA).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi300 – 3001Manganese 1 By similarity
Metal bindingi302 – 3021Manganese 1 By similarity
Metal bindingi329 – 3291Manganese 1 By similarity
Metal bindingi329 – 3291Manganese 2 By similarity
Metal bindingi361 – 3611Manganese 2 By similarity
Active sitei362 – 3621Proton donor By similarity
Metal bindingi410 – 4101Manganese 2 By similarity
Metal bindingi485 – 4851Manganese 2 By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. phosphoprotein phosphatase activity Source: UniProtKB-KW

GO - Biological processi

  1. protein dephosphorylation Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Manganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase 5 (EC:3.1.3.16)
Alternative name(s):
LePP5
Gene namesi
Name:PP5
OrganismiSolanum lycopersicum (Tomato) (Lycopersicon esculentum)
Taxonomic identifieri4081 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeSolaneaeSolanumLycopersicon
ProteomesiUP000004994: Unplaced

Subcellular locationi

Isoform 1 : Endoplasmic reticulum membrane; Multi-pass membrane protein. Nucleus membrane; Multi-pass membrane protein 1 Publication
Isoform 2 : Cytoplasm. Nucleusnucleoplasm. Nucleus speckle
Note: Cytoplasmic in darkness, but translocated to the nucleus upon illumination, when associated with phytochromes into speckles By similarity.1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei171 – 19121Helical; Reviewed predictionAdd
BLAST
Transmembranei192 – 21221Helical; Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
  3. nuclear membrane Source: UniProtKB-SubCell
  4. nuclear speck Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Membrane, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi411 – 4111G → R: Loss of activity; in isoform 2. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 556556Serine/threonine-protein phosphatase 5PRO_0000308995Add
BLAST

Expressioni

Tissue specificityi

Expressed in roots, stems, leaves, flowers, and fruits.1 Publication

Interactioni

Subunit structurei

Interacts with PHYA and PHYB, mostly when they are phosphorylated and in Pfr forms By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ84K11.
SMRiQ84K11. Positions 9-159, 233-554.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati14 – 4734TPR 1Add
BLAST
Repeati49 – 8133TPR 2Add
BLAST
Repeati82 – 11534TPR 3Add
BLAST

Domaini

TPR repeats are required for the binding with phytochromes By similarity.

Sequence similaritiesi

Contains 3 TPR repeats.

Keywords - Domaini

Repeat, TPR repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

KOiK04460.

Family and domain databases

Gene3Di1.25.40.10. 1 hit.
3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR013235. PPP_dom.
IPR006186. Ser/Thr-sp_prot-phosphatase.
IPR011236. Ser/Thr_PPase_5.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical.
IPR001440. TPR_1.
IPR019734. TPR_repeat.
[Graphical view]
PANTHERiPTHR11668:SF12. PTHR11668:SF12. 1 hit.
PfamiPF00149. Metallophos. 1 hit.
PF08321. PPP5. 2 hits.
PF00515. TPR_1. 1 hit.
[Graphical view]
PIRSFiPIRSF033096. PPPtase_5. 1 hit.
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
SM00028. TPR. 3 hits.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS50005. TPR. 3 hits.
PS50293. TPR_REGION. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q84K11-1) [UniParc]FASTAAdd to Basket

Also known as: 62 kDa isoform

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MPGMEAENSN ASRAEELKQL ANEAFKGHKY SQAIDLYTQA IELNGENAVY    50
YANRAFAHTK LEEYGSAIQD GTRAIEIDPR YSKGYYRRGA AYLAMGKFKD 100
ALKDFQQVKK LCPNDPDATK KLKECEKAVM KLKFEEAISV PESQRRSVAD 150
SIDYRSVGSG PGSSYVPTKT TAVSAAAALM GVLVVYMGTK AATMVAAAAS 200
AALLVVLITF LWGRCSDGFF TKSRTLELEV EPQYAGARIE GDVVTLDFVK 250
KMLDDFKNQK NLHKRYAYQI VLQTREMLRA LPSLVDIVVP EGKHFTVCGD 300
VHGQFYDLLN IFELNGLPSE DNPYLFNGDF VDRGSFSLEV ILTLFAFKCM 350
CPSAIHLARG NHESKSMNKI YGFEGEVRSK LSEIFVELFA EVFCCLPLAH 400
VINEKVFVVH GGLFSVDGVK LSDIRAIDRF CEPPEEGLMC ELLWSDPQPQ 450
PGRGPSKRGV GLSFGGDVTK RFLQENNLDL VVRSHEVKDE GYEIEHDGKL 500
ITVFSAPNYC DQMGNKGAFI RFEAPDMKPN IVTFSAVPHP DVKPMAYANN 550
FLRMFS 556
Length:556
Mass (Da):61,860
Last modified:June 1, 2003 - v1
Checksum:i39262299C9B27866
GO
Isoform 2 (identifier: Q84K11-2) [UniParc]FASTAAdd to Basket

Also known as: 55 kDa isoform

The sequence of this isoform differs from the canonical sequence as follows:
     158-228: Missing.

Note: Partial isoform 2 (148-485) lacking TPR repeats exhibits enhanced activity at pH 7.5 but not at pH 5.0 with phosphocasein as substrate. This partial protein is in addition inhibited by okadaic acid.

Show »
Length:485
Mass (Da):54,720
Checksum:iFCF68708D5520E68
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei158 – 22871Missing in isoform 2. VSP_029090Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY150041 mRNA. Translation: AAN64317.1.
AY182777 mRNA. Translation: AAO26213.1.
AY182778 Genomic DNA. Translation: AAO26214.1.
AY182778 Genomic DNA. Translation: AAO26215.1.
RefSeqiNP_001234232.1. NM_001247303.1. [Q84K11-2]
UniGeneiLes.3965.

Genome annotation databases

GeneIDi543849.
KEGGisly:543849.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY150041 mRNA. Translation: AAN64317.1 .
AY182777 mRNA. Translation: AAO26213.1 .
AY182778 Genomic DNA. Translation: AAO26214.1 .
AY182778 Genomic DNA. Translation: AAO26215.1 .
RefSeqi NP_001234232.1. NM_001247303.1. [Q84K11-2 ]
UniGenei Les.3965.

3D structure databases

ProteinModelPortali Q84K11.
SMRi Q84K11. Positions 9-159, 233-554.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 543849.
KEGGi sly:543849.

Phylogenomic databases

KOi K04460.

Family and domain databases

Gene3Di 1.25.40.10. 1 hit.
3.60.21.10. 1 hit.
InterProi IPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR013235. PPP_dom.
IPR006186. Ser/Thr-sp_prot-phosphatase.
IPR011236. Ser/Thr_PPase_5.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical.
IPR001440. TPR_1.
IPR019734. TPR_repeat.
[Graphical view ]
PANTHERi PTHR11668:SF12. PTHR11668:SF12. 1 hit.
Pfami PF00149. Metallophos. 1 hit.
PF08321. PPP5. 2 hits.
PF00515. TPR_1. 1 hit.
[Graphical view ]
PIRSFi PIRSF033096. PPPtase_5. 1 hit.
PRINTSi PR00114. STPHPHTASE.
SMARTi SM00156. PP2Ac. 1 hit.
SM00028. TPR. 3 hits.
[Graphical view ]
SUPFAMi SSF56300. SSF56300. 1 hit.
PROSITEi PS50005. TPR. 3 hits.
PS50293. TPR_REGION. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The subcellular localization of plant protein phosphatase 5 isoforms is determined by alternative splicing."
    de la Fuente van Bentem S., Vossen J.H., Vermeer J.E.M., de Vroomen M.J., Gadella T.W.J. Jr., Haring M.A., Cornelissen B.J.C.
    Plant Physiol. 133:702-712(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 1 AND 2), MUTAGENESIS OF GLY-411, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, ALTERNATIVE PRODUCTS, ACTIVATION BY FATTY ACIDS.
    Strain: cv. GCR161 and cv. VFN8.

Entry informationi

Entry nameiPPP5_SOLLC
AccessioniPrimary (citable) accession number: Q84K11
Secondary accession number(s): Q8H1H4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: June 1, 2003
Last modified: June 11, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi