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Q84JU4

- IBR5_ARATH

UniProt

Q84JU4 - IBR5_ARATH

Protein

Protein-tyrosine-phosphatase IBR5

Gene

IBR5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 87 (01 Oct 2014)
      Sequence version 1 (01 Jun 2003)
      Previous versions | rss
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    Functioni

    Required for the transduction of auxin and abscisic acid (ABA) signaling pathways. Dephosphorylates and inactivates the MAP kinase MPK12.4 Publications

    Catalytic activityi

    Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei129 – 1291Phosphocysteine intermediatePROSITE-ProRule annotation

    GO - Molecular functioni

    1. MAP kinase phosphatase activity Source: TAIR
    2. protein binding Source: UniProtKB
    3. protein tyrosine/serine/threonine phosphatase activity Source: RefGenome
    4. protein tyrosine phosphatase activity Source: UniProtKB-EC

    GO - Biological processi

    1. abscisic acid-activated signaling pathway Source: UniProtKB-KW
    2. auxin-activated signaling pathway Source: UniProtKB-KW
    3. inactivation of MAPK activity Source: GOC
    4. negative regulation of MAP kinase activity Source: TAIR
    5. protein dephosphorylation Source: RefGenome
    6. response to abscisic acid Source: TAIR
    7. response to auxin Source: TAIR

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Abscisic acid signaling pathway, Auxin signaling pathway

    Enzyme and pathway databases

    BioCyciARA:AT2G04550-MONOMER.
    ARA:GQT-2631-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein-tyrosine-phosphatase IBR5 (EC:3.1.3.48)
    Alternative name(s):
    Protein INDOLE-3-BUTYRIC ACID RESPONSE 5
    Short name:
    Protein IBA RESPONSE 5
    SKP1-interacting partner 33
    Gene namesi
    Name:IBR5
    Synonyms:SKIP33
    Ordered Locus Names:At2g04550
    ORF Names:T1O3.4
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G04550.

    Subcellular locationi

    Nucleus 1 Publication

    GO - Cellular componenti

    1. nucleus Source: TAIR

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    Impaired responses to phytohormones such as indole-3-butyric acid, indole-3-acetic acid (auxin), synthetic auxins, auxin transport inhibitors, and abscisic acid (ABA). Plants exhibit long roots and short hypocotyls when grown in the light, with aberrant vascular patterning, increased leaf serration, and reduced accumulation of auxin-inducible genes.2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 257257Protein-tyrosine-phosphatase IBR5PRO_0000375243Add
    BLAST

    Proteomic databases

    PaxDbiQ84JU4.
    PRIDEiQ84JU4.

    Expressioni

    Tissue specificityi

    Expressed in root tips and vasculature, cotyledons, stems, leaves vasculature and hydathodes, flowers, siliques, and seeds.1 Publication

    Developmental stagei

    During flower development, detected in sepals, anther filaments, and carpels. During germination, levels decline slightly two days after imbibition.1 Publication

    Gene expression databases

    GenevestigatoriQ84JU4.

    Interactioni

    Subunit structurei

    Interacts with SKP1A/ASK1 and with MPK12.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    MPK12Q8GYQ58EBI-604555,EBI-2128461

    Protein-protein interaction databases

    BioGridi398. 3 interactions.
    IntActiQ84JU4. 3 interactions.
    STRINGi3702.AT2G04550.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ84JU4.
    SMRiQ84JU4. Positions 50-185.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini50 – 234185Tyrosine-protein phosphataseAdd
    BLAST

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiNOG251782.
    HOGENOMiHOG000006127.
    InParanoidiQ84JU4.
    KOiK04459.
    OMAiCEKDKAR.
    PhylomeDBiQ84JU4.

    Family and domain databases

    Gene3Di3.90.190.10. 1 hit.
    InterProiIPR000340. Dual-sp_phosphatase_cat-dom.
    IPR020422. Dual-sp_phosphatase_subgr_cat.
    IPR024950. DUSP.
    IPR029021. Prot-tyrosine_phosphatase-like.
    IPR000387. Tyr/Dual-sp_Pase.
    IPR016130. Tyr_Pase_AS.
    [Graphical view]
    PANTHERiPTHR10159. PTHR10159. 1 hit.
    PfamiPF00782. DSPc. 1 hit.
    [Graphical view]
    SMARTiSM00195. DSPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52799. SSF52799. 1 hit.
    PROSITEiPS00383. TYR_PHOSPHATASE_1. 1 hit.
    PS50056. TYR_PHOSPHATASE_2. 1 hit.
    PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q84JU4-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MRKRERENPC SICGHYHKYE EGEVCGVCGH CMPVSSDTVA PQQVHVSAFP    50
    SEILPEFLYL GSYDNASRSE LLKTQGISRV LNTVPMCQNL YRNSFTYHGL 100
    DNEKVLQFDD AIKFLDQCEK DKARVLVHCM SGKSRSPAVV VAYLMKRKGW 150
    RLAESHQWVK QRRPSTDISP EFYQQLQEFE QGIFGSEMMS AMNINDAPTF 200
    GFGFPKIDNQ AQAPVFNNAP TSSIFSSPAS SIPPQEFTFG ATPPKPTTGG 250
    DIAMDGS 257
    Length:257
    Mass (Da):28,690
    Last modified:June 1, 2003 - v1
    Checksum:i68CFA8A26DC933DF
    GO
    Isoform 2 (identifier: Q84JU4-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         171-257: EFYQQLQEFE...TGGDIAMDGS → VLPTTAGV

    Note: Derived from EST data. No experimental confirmation available.

    Show »
    Length:178
    Mass (Da):20,162
    Checksum:i23A9C7E6D0E68B12
    GO

    Sequence cautioni

    The sequence AAD25825.1 differs from that shown. Reason: Erroneous gene model prediction.

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei171 – 25787EFYQQ…AMDGS → VLPTTAGV in isoform 2. CuratedVSP_037361Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BT004283 mRNA. Translation: AAO42283.1.
    AC006951 Genomic DNA. Translation: AAD25825.1. Sequence problems.
    CP002685 Genomic DNA. Translation: AEC05845.1.
    CP002685 Genomic DNA. Translation: AEC05846.1.
    BT005609 mRNA. Translation: AAO64029.1.
    AY337455 mRNA. Translation: AAR04550.1.
    PIRiG84458.
    RefSeqiNP_178534.2. NM_126486.5. [Q84JU4-1]
    NP_973418.2. NM_201689.3. [Q84JU4-2]
    UniGeneiAt.41296.

    Genome annotation databases

    EnsemblPlantsiAT2G04550.1; AT2G04550.1; AT2G04550. [Q84JU4-1]
    GeneIDi814997.
    KEGGiath:AT2G04550.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BT004283 mRNA. Translation: AAO42283.1 .
    AC006951 Genomic DNA. Translation: AAD25825.1 . Sequence problems.
    CP002685 Genomic DNA. Translation: AEC05845.1 .
    CP002685 Genomic DNA. Translation: AEC05846.1 .
    BT005609 mRNA. Translation: AAO64029.1 .
    AY337455 mRNA. Translation: AAR04550.1 .
    PIRi G84458.
    RefSeqi NP_178534.2. NM_126486.5. [Q84JU4-1 ]
    NP_973418.2. NM_201689.3. [Q84JU4-2 ]
    UniGenei At.41296.

    3D structure databases

    ProteinModelPortali Q84JU4.
    SMRi Q84JU4. Positions 50-185.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 398. 3 interactions.
    IntActi Q84JU4. 3 interactions.
    STRINGi 3702.AT2G04550.1-P.

    Proteomic databases

    PaxDbi Q84JU4.
    PRIDEi Q84JU4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G04550.1 ; AT2G04550.1 ; AT2G04550 . [Q84JU4-1 ]
    GeneIDi 814997.
    KEGGi ath:AT2G04550.

    Organism-specific databases

    TAIRi AT2G04550.

    Phylogenomic databases

    eggNOGi NOG251782.
    HOGENOMi HOG000006127.
    InParanoidi Q84JU4.
    KOi K04459.
    OMAi CEKDKAR.
    PhylomeDBi Q84JU4.

    Enzyme and pathway databases

    BioCyci ARA:AT2G04550-MONOMER.
    ARA:GQT-2631-MONOMER.

    Gene expression databases

    Genevestigatori Q84JU4.

    Family and domain databases

    Gene3Di 3.90.190.10. 1 hit.
    InterProi IPR000340. Dual-sp_phosphatase_cat-dom.
    IPR020422. Dual-sp_phosphatase_subgr_cat.
    IPR024950. DUSP.
    IPR029021. Prot-tyrosine_phosphatase-like.
    IPR000387. Tyr/Dual-sp_Pase.
    IPR016130. Tyr_Pase_AS.
    [Graphical view ]
    PANTHERi PTHR10159. PTHR10159. 1 hit.
    Pfami PF00782. DSPc. 1 hit.
    [Graphical view ]
    SMARTi SM00195. DSPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52799. SSF52799. 1 hit.
    PROSITEi PS00383. TYR_PHOSPHATASE_1. 1 hit.
    PS50056. TYR_PHOSPHATASE_2. 1 hit.
    PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "IBR5, a dual-specificity phosphatase-like protein modulating auxin and abscisic acid responsiveness in Arabidopsis."
      Monroe-Augustus M., Zolman B.K., Bartel B.
      Plant Cell 15:2979-2991(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
      Strain: cv. Landsberg erecta.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: cv. Columbia.
    5. "Protein interaction analysis of SCF ubiquitin E3 ligase subunits from Arabidopsis."
      Risseeuw E.P., Daskalchuk T.E., Banks T.W., Liu E., Cotelesage J., Hellmann H., Estelle M., Somers D.E., Crosby W.L.
      Plant J. 34:753-767(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SKP1A/ASK1.
    6. "The IBR5 phosphatase promotes Arabidopsis auxin responses through a novel mechanism distinct from TIR1-mediated repressor degradation."
      Strader L.C., Monroe-Augustus M., Bartel B.
      BMC Plant Biol. 8:41-41(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE.
    7. "Arabidopsis iba response5 suppressors separate responses to various hormones."
      Strader L.C., Monroe-Augustus M., Rogers K.C., Lin G.L., Bartel B.
      Genetics 180:2019-2031(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    8. "Arabidopsis mitogen-activated protein kinase MPK12 interacts with the MAPK phosphatase IBR5 and regulates auxin signaling."
      Lee J.S., Wang S., Sritubtim S., Chen J.-G., Ellis B.E.
      Plant J. 57:975-985(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH MPK12.

    Entry informationi

    Entry nameiIBR5_ARATH
    AccessioniPrimary (citable) accession number: Q84JU4
    Secondary accession number(s): Q3EC68, Q9SJB6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 26, 2009
    Last sequence update: June 1, 2003
    Last modified: October 1, 2014
    This is version 87 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3