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Q84JU4

- IBR5_ARATH

UniProt

Q84JU4 - IBR5_ARATH

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Protein
Protein-tyrosine-phosphatase IBR5
Gene
IBR5, SKIP33, At2g04550, T1O3.4
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Required for the transduction of auxin and abscisic acid (ABA) signaling pathways. Dephosphorylates and inactivates the MAP kinase MPK12.4 Publications

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei129 – 1291Phosphocysteine intermediate By similarity

GO - Molecular functioni

  1. MAP kinase phosphatase activity Source: TAIR
  2. protein binding Source: UniProtKB
  3. protein tyrosine phosphatase activity Source: UniProtKB-EC
  4. protein tyrosine/serine/threonine phosphatase activity Source: RefGenome
Complete GO annotation...

GO - Biological processi

  1. abscisic acid-activated signaling pathway Source: UniProtKB-KW
  2. auxin-activated signaling pathway Source: UniProtKB-KW
  3. inactivation of MAPK activity Source: GOC
  4. negative regulation of MAP kinase activity Source: TAIR
  5. protein dephosphorylation Source: RefGenome
  6. response to abscisic acid Source: TAIR
  7. response to auxin Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Abscisic acid signaling pathway, Auxin signaling pathway

Enzyme and pathway databases

BioCyciARA:AT2G04550-MONOMER.
ARA:GQT-2631-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein-tyrosine-phosphatase IBR5 (EC:3.1.3.48)
Alternative name(s):
Protein INDOLE-3-BUTYRIC ACID RESPONSE 5
Short name:
Protein IBA RESPONSE 5
SKP1-interacting partner 33
Gene namesi
Name:IBR5
Synonyms:SKIP33
Ordered Locus Names:At2g04550
ORF Names:T1O3.4
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G04550.

Subcellular locationi

Nucleus 1 Publication

GO - Cellular componenti

  1. nucleus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Impaired responses to phytohormones such as indole-3-butyric acid, indole-3-acetic acid (auxin), synthetic auxins, auxin transport inhibitors, and abscisic acid (ABA). Plants exhibit long roots and short hypocotyls when grown in the light, with aberrant vascular patterning, increased leaf serration, and reduced accumulation of auxin-inducible genes.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 257257Protein-tyrosine-phosphatase IBR5
PRO_0000375243Add
BLAST

Proteomic databases

PaxDbiQ84JU4.
PRIDEiQ84JU4.

Expressioni

Tissue specificityi

Expressed in root tips and vasculature, cotyledons, stems, leaves vasculature and hydathodes, flowers, siliques, and seeds.1 Publication

Developmental stagei

During flower development, detected in sepals, anther filaments, and carpels. During germination, levels decline slightly two days after imbibition.1 Publication

Gene expression databases

GenevestigatoriQ84JU4.

Interactioni

Subunit structurei

Interacts with SKP1A/ASK1 and with MPK12.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
MPK12Q8GYQ58EBI-604555,EBI-2128461

Protein-protein interaction databases

BioGridi398. 3 interactions.
IntActiQ84JU4. 3 interactions.
STRINGi3702.AT2G04550.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ84JU4.
SMRiQ84JU4. Positions 50-185.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini50 – 234185Tyrosine-protein phosphatase
Add
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG251782.
HOGENOMiHOG000006127.
InParanoidiQ84JU4.
KOiK04459.
OMAiCEKDKAR.
PhylomeDBiQ84JU4.

Family and domain databases

Gene3Di3.90.190.10. 1 hit.
InterProiIPR000340. Dual-sp_phosphatase_cat-dom.
IPR020422. Dual-sp_phosphatase_subgr_cat.
IPR024950. DUSP.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
[Graphical view]
PANTHERiPTHR10159. PTHR10159. 1 hit.
PfamiPF00782. DSPc. 1 hit.
[Graphical view]
SMARTiSM00195. DSPc. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 1 hit.
PROSITEiPS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q84JU4-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MRKRERENPC SICGHYHKYE EGEVCGVCGH CMPVSSDTVA PQQVHVSAFP    50
SEILPEFLYL GSYDNASRSE LLKTQGISRV LNTVPMCQNL YRNSFTYHGL 100
DNEKVLQFDD AIKFLDQCEK DKARVLVHCM SGKSRSPAVV VAYLMKRKGW 150
RLAESHQWVK QRRPSTDISP EFYQQLQEFE QGIFGSEMMS AMNINDAPTF 200
GFGFPKIDNQ AQAPVFNNAP TSSIFSSPAS SIPPQEFTFG ATPPKPTTGG 250
DIAMDGS 257
Length:257
Mass (Da):28,690
Last modified:June 1, 2003 - v1
Checksum:i68CFA8A26DC933DF
GO
Isoform 2 (identifier: Q84JU4-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     171-257: EFYQQLQEFE...TGGDIAMDGS → VLPTTAGV

Note: Derived from EST data. No experimental confirmation available.

Show »
Length:178
Mass (Da):20,162
Checksum:i23A9C7E6D0E68B12
GO

Sequence cautioni

The sequence AAD25825.1 differs from that shown. Reason: Erroneous gene model prediction.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei171 – 25787EFYQQ…AMDGS → VLPTTAGV in isoform 2.
VSP_037361Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BT004283 mRNA. Translation: AAO42283.1.
AC006951 Genomic DNA. Translation: AAD25825.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC05845.1.
CP002685 Genomic DNA. Translation: AEC05846.1.
BT005609 mRNA. Translation: AAO64029.1.
AY337455 mRNA. Translation: AAR04550.1.
PIRiG84458.
RefSeqiNP_178534.2. NM_126486.5. [Q84JU4-1]
NP_973418.2. NM_201689.3. [Q84JU4-2]
UniGeneiAt.41296.

Genome annotation databases

EnsemblPlantsiAT2G04550.1; AT2G04550.1; AT2G04550. [Q84JU4-1]
GeneIDi814997.
KEGGiath:AT2G04550.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BT004283 mRNA. Translation: AAO42283.1 .
AC006951 Genomic DNA. Translation: AAD25825.1 . Sequence problems.
CP002685 Genomic DNA. Translation: AEC05845.1 .
CP002685 Genomic DNA. Translation: AEC05846.1 .
BT005609 mRNA. Translation: AAO64029.1 .
AY337455 mRNA. Translation: AAR04550.1 .
PIRi G84458.
RefSeqi NP_178534.2. NM_126486.5. [Q84JU4-1 ]
NP_973418.2. NM_201689.3. [Q84JU4-2 ]
UniGenei At.41296.

3D structure databases

ProteinModelPortali Q84JU4.
SMRi Q84JU4. Positions 50-185.
ModBasei Search...

Protein-protein interaction databases

BioGridi 398. 3 interactions.
IntActi Q84JU4. 3 interactions.
STRINGi 3702.AT2G04550.1-P.

Proteomic databases

PaxDbi Q84JU4.
PRIDEi Q84JU4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G04550.1 ; AT2G04550.1 ; AT2G04550 . [Q84JU4-1 ]
GeneIDi 814997.
KEGGi ath:AT2G04550.

Organism-specific databases

TAIRi AT2G04550.

Phylogenomic databases

eggNOGi NOG251782.
HOGENOMi HOG000006127.
InParanoidi Q84JU4.
KOi K04459.
OMAi CEKDKAR.
PhylomeDBi Q84JU4.

Enzyme and pathway databases

BioCyci ARA:AT2G04550-MONOMER.
ARA:GQT-2631-MONOMER.

Gene expression databases

Genevestigatori Q84JU4.

Family and domain databases

Gene3Di 3.90.190.10. 1 hit.
InterProi IPR000340. Dual-sp_phosphatase_cat-dom.
IPR020422. Dual-sp_phosphatase_subgr_cat.
IPR024950. DUSP.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
[Graphical view ]
PANTHERi PTHR10159. PTHR10159. 1 hit.
Pfami PF00782. DSPc. 1 hit.
[Graphical view ]
SMARTi SM00195. DSPc. 1 hit.
[Graphical view ]
SUPFAMi SSF52799. SSF52799. 1 hit.
PROSITEi PS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "IBR5, a dual-specificity phosphatase-like protein modulating auxin and abscisic acid responsiveness in Arabidopsis."
    Monroe-Augustus M., Zolman B.K., Bartel B.
    Plant Cell 15:2979-2991(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Strain: cv. Landsberg erecta.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  5. "Protein interaction analysis of SCF ubiquitin E3 ligase subunits from Arabidopsis."
    Risseeuw E.P., Daskalchuk T.E., Banks T.W., Liu E., Cotelesage J., Hellmann H., Estelle M., Somers D.E., Crosby W.L.
    Plant J. 34:753-767(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SKP1A/ASK1.
  6. "The IBR5 phosphatase promotes Arabidopsis auxin responses through a novel mechanism distinct from TIR1-mediated repressor degradation."
    Strader L.C., Monroe-Augustus M., Bartel B.
    BMC Plant Biol. 8:41-41(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  7. "Arabidopsis iba response5 suppressors separate responses to various hormones."
    Strader L.C., Monroe-Augustus M., Rogers K.C., Lin G.L., Bartel B.
    Genetics 180:2019-2031(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "Arabidopsis mitogen-activated protein kinase MPK12 interacts with the MAPK phosphatase IBR5 and regulates auxin signaling."
    Lee J.S., Wang S., Sritubtim S., Chen J.-G., Ellis B.E.
    Plant J. 57:975-985(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH MPK12.

Entry informationi

Entry nameiIBR5_ARATH
AccessioniPrimary (citable) accession number: Q84JU4
Secondary accession number(s): Q3EC68, Q9SJB6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 26, 2009
Last sequence update: June 1, 2003
Last modified: June 11, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi