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Protein

Protein-tyrosine-phosphatase IBR5

Gene

IBR5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for the transduction of auxin and abscisic acid (ABA) signaling pathways. Dephosphorylates and inactivates the MAP kinase MPK12.4 Publications

Caution

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei129Phosphocysteine intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • abscisic acid-activated signaling pathway Source: UniProtKB-KW
  • auxin-activated signaling pathway Source: UniProtKB-KW
  • intracellular signal transduction Source: TAIR
  • negative regulation of MAP kinase activity Source: TAIR
  • regulation of organ growth Source: TAIR
  • regulation of rate of cell growth Source: TAIR
  • response to abscisic acid Source: TAIR
  • response to auxin Source: TAIR

Keywordsi

Molecular functionHydrolase
Biological processAbscisic acid signaling pathway, Auxin signaling pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Protein-tyrosine-phosphatase IBR5 (EC:3.1.3.48)
Alternative name(s):
Protein INDOLE-3-BUTYRIC ACID RESPONSE 5
Short name:
Protein IBA RESPONSE 5
SKP1-interacting partner 33
Gene namesi
Name:IBR5
Synonyms:SKIP33
Ordered Locus Names:At2g04550
ORF Names:T1O3.4
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G04550
TAIRilocus:2058344 AT2G04550

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Impaired responses to phytohormones such as indole-3-butyric acid, indole-3-acetic acid (auxin), synthetic auxins, auxin transport inhibitors, and abscisic acid (ABA). Plants exhibit long roots and short hypocotyls when grown in the light, with aberrant vascular patterning, increased leaf serration, and reduced accumulation of auxin-inducible genes.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003752431 – 257Protein-tyrosine-phosphatase IBR5Add BLAST257

Proteomic databases

PaxDbiQ84JU4

Expressioni

Tissue specificityi

Expressed in root tips and vasculature, cotyledons, stems, leaves vasculature and hydathodes, flowers, siliques, and seeds.1 Publication

Developmental stagei

During flower development, detected in sepals, anther filaments, and carpels. During germination, levels decline slightly two days after imbibition.1 Publication

Gene expression databases

ExpressionAtlasiQ84JU4 baseline and differential
GenevisibleiQ84JU4 AT

Interactioni

Subunit structurei

Interacts with SKP1A/ASK1 and with MPK12.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
MPK12Q8GYQ58EBI-604555,EBI-2128461

Protein-protein interaction databases

BioGridi3983 interactors.
IntActiQ84JU4 3 interactors.
STRINGi3702.AT2G04550.1

Structurei

3D structure databases

ProteinModelPortaliQ84JU4
SMRiQ84JU4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini50 – 234Tyrosine-protein phosphataseAdd BLAST185

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1716 Eukaryota
COG2453 LUCA
HOGENOMiHOG000006127
InParanoidiQ84JU4
KOiK04459
OMAiMNINDAP
OrthoDBiEOG09360K7C
PhylomeDBiQ84JU4

Family and domain databases

CDDicd00127 DSPc, 1 hit
Gene3Di3.90.190.101 hit
InterProiView protein in InterPro
IPR000340 Dual-sp_phosphatase_cat-dom
IPR024950 DUSP
IPR029021 Prot-tyrosine_phosphatase-like
IPR016130 Tyr_Pase_AS
IPR000387 TYR_PHOSPHATASE_dom
IPR020422 TYR_PHOSPHATASE_DUAL_dom
PANTHERiPTHR10159 PTHR10159, 1 hit
PfamiView protein in Pfam
PF00782 DSPc, 1 hit
SMARTiView protein in SMART
SM00195 DSPc, 1 hit
SUPFAMiSSF52799 SSF52799, 1 hit
PROSITEiView protein in PROSITE
PS00383 TYR_PHOSPHATASE_1, 1 hit
PS50056 TYR_PHOSPHATASE_2, 1 hit
PS50054 TYR_PHOSPHATASE_DUAL, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q84JU4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRKRERENPC SICGHYHKYE EGEVCGVCGH CMPVSSDTVA PQQVHVSAFP
60 70 80 90 100
SEILPEFLYL GSYDNASRSE LLKTQGISRV LNTVPMCQNL YRNSFTYHGL
110 120 130 140 150
DNEKVLQFDD AIKFLDQCEK DKARVLVHCM SGKSRSPAVV VAYLMKRKGW
160 170 180 190 200
RLAESHQWVK QRRPSTDISP EFYQQLQEFE QGIFGSEMMS AMNINDAPTF
210 220 230 240 250
GFGFPKIDNQ AQAPVFNNAP TSSIFSSPAS SIPPQEFTFG ATPPKPTTGG

DIAMDGS
Length:257
Mass (Da):28,690
Last modified:June 1, 2003 - v1
Checksum:i68CFA8A26DC933DF
GO
Isoform 2 (identifier: Q84JU4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     171-257: EFYQQLQEFE...TGGDIAMDGS → VLPTTAGV

Note: Derived from EST data. No experimental confirmation available.
Show »
Length:178
Mass (Da):20,162
Checksum:i23A9C7E6D0E68B12
GO

Sequence cautioni

The sequence AAD25825 differs from that shown. Reason: Erroneous gene model prediction.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_037361171 – 257EFYQQ…AMDGS → VLPTTAGV in isoform 2. CuratedAdd BLAST87

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT004283 mRNA Translation: AAO42283.1
AC006951 Genomic DNA Translation: AAD25825.1 Sequence problems.
CP002685 Genomic DNA Translation: AEC05845.1
CP002685 Genomic DNA Translation: AEC05846.1
BT005609 mRNA Translation: AAO64029.1
AY337455 mRNA Translation: AAR04550.1
PIRiG84458
RefSeqiNP_178534.2, NM_126486.6 [Q84JU4-1]
NP_973418.2, NM_201689.3 [Q84JU4-2]
UniGeneiAt.41296

Genome annotation databases

EnsemblPlantsiAT2G04550.1; AT2G04550.1; AT2G04550 [Q84JU4-1]
AT2G04550.3; AT2G04550.3; AT2G04550 [Q84JU4-2]
GeneIDi814997
GrameneiAT2G04550.1; AT2G04550.1; AT2G04550 [Q84JU4-1]
AT2G04550.3; AT2G04550.3; AT2G04550 [Q84JU4-2]
KEGGiath:AT2G04550

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiIBR5_ARATH
AccessioniPrimary (citable) accession number: Q84JU4
Secondary accession number(s): Q3EC68, Q9SJB6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 26, 2009
Last sequence update: June 1, 2003
Last modified: April 25, 2018
This is version 111 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome