Q84JU4 (IBR5_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein-tyrosine-phosphatase IBR5
EC=3.1.3.48

Alternative name(s):
Protein INDOLE-3-BUTYRIC ACID RESPONSE 5
Short name=Protein IBA RESPONSE 5
SKP1-interacting partner 33

Gene names

Name:	IBR5
Synonyms:	SKIP33
Ordered Locus Names:	At2g04550
ORF Names:	T1O3.4

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	257 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of SCF(ASK-cullin-F-box) E3 ubiquitin ligase complexes, which may mediate the ubiquitination and subsequent proteasomal degradation of target proteins By similarity. Required for the transduction of auxin and abscisic acid (ABA) signaling pathways. Dephosphorylates and inactivates the MAP kinase MPK12. Ref.1 Ref.6 Ref.7 Ref.8
Catalytic activity	Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Part of a SCF (ASK-cullin-F-box) protein ligase complex By similarity. Interacts with SKP1A/ASK1 and with MPK12. Ref.5 Ref.8
Subcellular location	Nucleus Ref.8.
Tissue specificity	Expressed in root tips and vasculature, cotyledons, stems, leaves vasculature and hydathodes, flowers, siliques, and seeds. Ref.1
Developmental stage	During flower development, detected in sepals, anther filaments, and carpels. During germination, levels decline slightly two days after imbibition. Ref.1
Domain	The F-box is necessary for the interaction with ASK proteins By similarity.
Disruption phenotype	Impaired responses to phytohormones such as indole-3-butyric acid, indole-3-acetic acid (auxin), synthetic auxins, auxin transport inhibitors, and abscisic acid (ABA). Plants exhibit long roots and short hypocotyls when grown in the light, with aberrant vascular patterning, increased leaf serration, and reduced accumulation of auxin-inducible genes. Ref.1 Ref.6
Sequence similarities	Belongs to the protein-tyrosine phosphatase family. Contains 1 F-box domain. Contains 1 tyrosine-protein phosphatase domain.
Sequence caution	The sequence AAD25825.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Abscisic acid signaling pathway Auxin signaling pathway
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	abscisic acid mediated signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW auxin mediated signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW intracellular protein kinase cascade Inferred by curator Ref.8. Source: TAIR peptidyl-tyrosine dephosphorylation Inferred from electronic annotation. Source: GOC protein ubiquitination Inferred from electronic annotation. Source: UniProtKB-UniPathway response to abscisic acid stimulus Inferred from mutant phenotype Ref.1. Source: TAIR response to auxin stimulus Inferred from mutant phenotype Ref.1. Source: TAIR
Cellular_component	nucleus Inferred from direct assay Ref.8. Source: TAIR
Molecular_function	MAP kinase phosphatase activity Inferred from direct assay Ref.8. Source: TAIR protein tyrosine phosphatase activity Inferred from electronic annotation. Source: EC protein tyrosine/serine/threonine phosphatase activity Inferred from Biological aspect of Ancestor. Source: RefGenome
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
MPK12	Q8GYQ5	8	EBI-604555,EBI-2128461

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q84JU4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q84JU4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     171-257: EFYQQLQEFE...TGGDIAMDGS → VLPTTAGV

Note: Derived from EST data. No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 257	257	Protein-tyrosine-phosphatase IBR5		PRO_0000375243
Regions
	Domain	1 – 49	49	F-box; degenerate
	Domain	50 – 234	185	Tyrosine-protein phosphatase
Sites
	Active site	129	1	Phosphocysteine intermediate By similarity
Natural variations
	Alternative sequence	171 – 257	87	EFYQQ…AMDGS → VLPTTAGV in isoform 2.		VSP_037361

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 68CFA8A26DC933DF	FASTA	257	28,690
	10 20 30 40 50 60 MRKRERENPC SICGHYHKYE EGEVCGVCGH CMPVSSDTVA PQQVHVSAFP SEILPEFLYL 70 80 90 100 110 120 GSYDNASRSE LLKTQGISRV LNTVPMCQNL YRNSFTYHGL DNEKVLQFDD AIKFLDQCEK 130 140 150 160 170 180 DKARVLVHCM SGKSRSPAVV VAYLMKRKGW RLAESHQWVK QRRPSTDISP EFYQQLQEFE 190 200 210 220 230 240 QGIFGSEMMS AMNINDAPTF GFGFPKIDNQ AQAPVFNNAP TSSIFSSPAS SIPPQEFTFG 250 ATPPKPTTGG DIAMDGS
	Isoform 2 [UniParc]. Checksum: 23A9C7E6D0E68B12	FASTA	178	20,162
	10 20 30 40 50 60 MRKRERENPC SICGHYHKYE EGEVCGVCGH CMPVSSDTVA PQQVHVSAFP SEILPEFLYL 70 80 90 100 110 120 GSYDNASRSE LLKTQGISRV LNTVPMCQNL YRNSFTYHGL DNEKVLQFDD AIKFLDQCEK 130 140 150 160 170 DKARVLVHCM SGKSRSPAVV VAYLMKRKGW RLAESHQWVK QRRPSTDISP VLPTTAGV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"IBR5, a dual-specificity phosphatase-like protein modulating auxin and abscisic acid responsiveness in Arabidopsis." Monroe-Augustus M., Zolman B.K., Bartel B. Plant Cell 15:2979-2991(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE. Strain: cv. Landsberg erecta.
[2]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: cv. Columbia.
[5]	"Protein interaction analysis of SCF ubiquitin E3 ligase subunits from Arabidopsis." Risseeuw E.P., Daskalchuk T.E., Banks T.W., Liu E., Cotelesage J., Hellmann H., Estelle M., Somers D.E., Crosby W.L. Plant J. 34:753-767(2003) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH SKP1A/ASK1.
[6]	"The IBR5 phosphatase promotes Arabidopsis auxin responses through a novel mechanism distinct from TIR1-mediated repressor degradation." Strader L.C., Monroe-Augustus M., Bartel B. BMC Plant Biol. 8:41-41(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[7]	"Arabidopsis iba response5 suppressors separate responses to various hormones." Strader L.C., Monroe-Augustus M., Rogers K.C., Lin G.L., Bartel B. Genetics 180:2019-2031(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[8]	"Arabidopsis mitogen-activated protein kinase MPK12 interacts with the MAPK phosphatase IBR5 and regulates auxin signaling." Lee J.S., Wang S., Sritubtim S., Chen J.-G., Ellis B.E. Plant J. 57:975-985(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH MPK12.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BT004283 mRNA. Translation: AAO42283.1. AC006951 Genomic DNA. Translation: AAD25825.1. Sequence problems. CP002685 Genomic DNA. Translation: AEC05845.1. CP002685 Genomic DNA. Translation: AEC05846.1. BT005609 mRNA. Translation: AAO64029.1. AY337455 mRNA. Translation: AAR04550.1.
IPI	IPI00528260. IPI00532503.
PIR	G84458.
RefSeq	NP_178534.2. NM_126486.5. NP_973418.2. NM_201689.3.
UniGene	At.41296.
3D structure databases
HSSP	HSSP built from PDB template 1M3G based on UniProtKB Q05923.
ProteinModelPortal	Q84JU4.
SMR	Q84JU4. Positions 50-185.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q84JU4. 3 interactions.
STRING	3702.AT2G04550.1-P.
Proteomic databases
PaxDb	Q84JU4.
PRIDE	Q84JU4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G04550.1; AT2G04550.1; AT2G04550.
GeneID	814997.
KEGG	ath:AT2G04550.
Organism-specific databases
TAIR	At2g04550.
Phylogenomic databases
eggNOG	NOG251782.
HOGENOM	HOG000006127.
InParanoid	Q84JU4.
KO	K04459.
OMA	CMSGKSR.
PhylomeDB	Q84JU4.
ProtClustDB	CLSN2681142.
Enzyme and pathway databases
UniPathway	UPA00143.
Gene expression databases
Genevestigator	Q84JU4.
Family and domain databases
InterPro	IPR000340. Dual-sp_phosphatase_cat-dom. IPR020422. Dual-sp_phosphatase_subgr_cat. IPR024950. DUSP. IPR000387. Tyr/Dual-sp_Pase. IPR016130. Tyr_Pase_AS. [Graphical view]
PANTHER	PTHR10159. PTHR10159. 1 hit.
Pfam	PF00782. DSPc. 1 hit. [Graphical view]
SMART	SM00195. DSPc. 1 hit. [Graphical view]
PROSITE	PS50181. FBOX. False negative. PS00383. TYR_PHOSPHATASE_1. 1 hit. PS50056. TYR_PHOSPHATASE_2. 1 hit. PS50054. TYR_PHOSPHATASE_DUAL. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IBR5_ARATH

Accession

Primary (citable) accession number: Q84JU4
Secondary accession number(s): Q3EC68, Q9SJB6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 26, 2009
Last sequence update:	June 1, 2003
Last modified:	May 1, 2013
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program