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Q84JU4 (IBR5_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein-tyrosine-phosphatase IBR5

EC=3.1.3.48
Alternative name(s):
Protein INDOLE-3-BUTYRIC ACID RESPONSE 5
Short name=Protein IBA RESPONSE 5
SKP1-interacting partner 33
Gene names
Name:IBR5
Synonyms:SKIP33
Ordered Locus Names:At2g04550
ORF Names:T1O3.4
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length257 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for the transduction of auxin and abscisic acid (ABA) signaling pathways. Dephosphorylates and inactivates the MAP kinase MPK12. Ref.1 Ref.6 Ref.7 Ref.8

Catalytic activity

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Subunit structure

Interacts with SKP1A/ASK1 and with MPK12. Ref.5 Ref.8

Subcellular location

Nucleus Ref.8.

Tissue specificity

Expressed in root tips and vasculature, cotyledons, stems, leaves vasculature and hydathodes, flowers, siliques, and seeds. Ref.1

Developmental stage

During flower development, detected in sepals, anther filaments, and carpels. During germination, levels decline slightly two days after imbibition. Ref.1

Disruption phenotype

Impaired responses to phytohormones such as indole-3-butyric acid, indole-3-acetic acid (auxin), synthetic auxins, auxin transport inhibitors, and abscisic acid (ABA). Plants exhibit long roots and short hypocotyls when grown in the light, with aberrant vascular patterning, increased leaf serration, and reduced accumulation of auxin-inducible genes. Ref.1 Ref.6

Sequence similarities

Belongs to the protein-tyrosine phosphatase family.

Contains 1 tyrosine-protein phosphatase domain.

Sequence caution

The sequence AAD25825.1 differs from that shown. Reason: Erroneous gene model prediction.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

MPK12Q8GYQ58EBI-604555,EBI-2128461

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q84JU4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q84JU4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     171-257: EFYQQLQEFE...TGGDIAMDGS → VLPTTAGV
Note: Derived from EST data. No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 257257Protein-tyrosine-phosphatase IBR5
PRO_0000375243

Regions

Domain50 – 234185Tyrosine-protein phosphatase

Sites

Active site1291Phosphocysteine intermediate By similarity

Natural variations

Alternative sequence171 – 25787EFYQQ…AMDGS → VLPTTAGV in isoform 2.
VSP_037361

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 68CFA8A26DC933DF

FASTA25728,690
        10         20         30         40         50         60 
MRKRERENPC SICGHYHKYE EGEVCGVCGH CMPVSSDTVA PQQVHVSAFP SEILPEFLYL 

        70         80         90        100        110        120 
GSYDNASRSE LLKTQGISRV LNTVPMCQNL YRNSFTYHGL DNEKVLQFDD AIKFLDQCEK 

       130        140        150        160        170        180 
DKARVLVHCM SGKSRSPAVV VAYLMKRKGW RLAESHQWVK QRRPSTDISP EFYQQLQEFE 

       190        200        210        220        230        240 
QGIFGSEMMS AMNINDAPTF GFGFPKIDNQ AQAPVFNNAP TSSIFSSPAS SIPPQEFTFG 

       250 
ATPPKPTTGG DIAMDGS 

« Hide

Isoform 2 [UniParc].

Checksum: 23A9C7E6D0E68B12
Show »

FASTA17820,162

References

« Hide 'large scale' references
[1]"IBR5, a dual-specificity phosphatase-like protein modulating auxin and abscisic acid responsiveness in Arabidopsis."
Monroe-Augustus M., Zolman B.K., Bartel B.
Plant Cell 15:2979-2991(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
Strain: cv. Landsberg erecta.
[2]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: cv. Columbia.
[5]"Protein interaction analysis of SCF ubiquitin E3 ligase subunits from Arabidopsis."
Risseeuw E.P., Daskalchuk T.E., Banks T.W., Liu E., Cotelesage J., Hellmann H., Estelle M., Somers D.E., Crosby W.L.
Plant J. 34:753-767(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SKP1A/ASK1.
[6]"The IBR5 phosphatase promotes Arabidopsis auxin responses through a novel mechanism distinct from TIR1-mediated repressor degradation."
Strader L.C., Monroe-Augustus M., Bartel B.
BMC Plant Biol. 8:41-41(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[7]"Arabidopsis iba response5 suppressors separate responses to various hormones."
Strader L.C., Monroe-Augustus M., Rogers K.C., Lin G.L., Bartel B.
Genetics 180:2019-2031(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[8]"Arabidopsis mitogen-activated protein kinase MPK12 interacts with the MAPK phosphatase IBR5 and regulates auxin signaling."
Lee J.S., Wang S., Sritubtim S., Chen J.-G., Ellis B.E.
Plant J. 57:975-985(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH MPK12.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BT004283 mRNA. Translation: AAO42283.1.
AC006951 Genomic DNA. Translation: AAD25825.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC05845.1.
CP002685 Genomic DNA. Translation: AEC05846.1.
BT005609 mRNA. Translation: AAO64029.1.
AY337455 mRNA. Translation: AAR04550.1.
PIRG84458.
RefSeqNP_178534.2. NM_126486.5. [Q84JU4-1]
NP_973418.2. NM_201689.3. [Q84JU4-2]
UniGeneAt.41296.

3D structure databases

ProteinModelPortalQ84JU4.
SMRQ84JU4. Positions 50-185.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid398. 3 interactions.
IntActQ84JU4. 3 interactions.
STRING3702.AT2G04550.1-P.

Proteomic databases

PaxDbQ84JU4.
PRIDEQ84JU4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G04550.1; AT2G04550.1; AT2G04550. [Q84JU4-1]
GeneID814997.
KEGGath:AT2G04550.

Organism-specific databases

TAIRAT2G04550.

Phylogenomic databases

eggNOGNOG251782.
HOGENOMHOG000006127.
InParanoidQ84JU4.
KOK04459.
OMACEKDKAR.
PhylomeDBQ84JU4.

Enzyme and pathway databases

BioCycARA:AT2G04550-MONOMER.
ARA:GQT-2631-MONOMER.

Gene expression databases

GenevestigatorQ84JU4.

Family and domain databases

Gene3D3.90.190.10. 1 hit.
InterProIPR000340. Dual-sp_phosphatase_cat-dom.
IPR020422. Dual-sp_phosphatase_subgr_cat.
IPR024950. DUSP.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
[Graphical view]
PANTHERPTHR10159. PTHR10159. 1 hit.
PfamPF00782. DSPc. 1 hit.
[Graphical view]
SMARTSM00195. DSPc. 1 hit.
[Graphical view]
SUPFAMSSF52799. SSF52799. 1 hit.
PROSITEPS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameIBR5_ARATH
AccessionPrimary (citable) accession number: Q84JU4
Secondary accession number(s): Q3EC68, Q9SJB6
Entry history
Integrated into UniProtKB/Swiss-Prot: May 26, 2009
Last sequence update: June 1, 2003
Last modified: June 11, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names